TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Perović, Tatjana
AU  - Manojlović, Dragan
AU  - Pavlović, Slađan
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6880
AB  - (1) Background: Multiple sclerosis (MS) is a chronic, complex, and demyelinating disease
closely associated with altered levels of trace elements. Although the first studies into the role of
trace elements in MS were published in the 1970s, for five decades it has remained unknown whether
trace elements can be part of this heterogeneous neurological disease. (2) Materials and methods:
To drive toward at a potential solution, we conducted a systematic review and meta-analysis to
elucidate whether there were differences in circulating levels of neurologically important essential
trace elements (Zn, Fe, Co, Cu, Mn, and Se) between MS cases and controls. (3) Results: This study
revealed significantly lower serum/plasma Zn and Fe levels and higher Cu levels in MS-affected
individuals compared to controls. At the same time, no significant differences were found between
the MS cases and controls regarding their serum/plasma levels of Co, Mn, or Se. Thus, the loss of Fe
and Zn should be considered in supplementation/nutrition strategies for MS patients. On the other
hand, since high serum Cu levels indicate a burden on the bloodstreams of MS patients, Cu should
be excluded from mineral supplement strategies. Furthermore, all three trace elements (Fe, Zn, and
Cu) should be considered from an etiological point of view, and, most importantly, their levels in the
bloodstreams of MS patients should be monitored. (4) Conclusions: This study highlights the way for
personalized and targeted strategies in the management of MS.
PB  - Basel: MDPI
T2  - Biomedicines
T1  - Changes of Target Essential Trace Elements in Multiple Sclerosis: A Systematic Review and Meta-Analysis
IS  - 1589
VL  - 12
DO  - 10.3390/biomedicines12071589
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Perović, Tatjana and Manojlović, Dragan and Pavlović, Slađan",
year = "2024",
abstract = "(1) Background: Multiple sclerosis (MS) is a chronic, complex, and demyelinating disease
closely associated with altered levels of trace elements. Although the first studies into the role of
trace elements in MS were published in the 1970s, for five decades it has remained unknown whether
trace elements can be part of this heterogeneous neurological disease. (2) Materials and methods:
To drive toward at a potential solution, we conducted a systematic review and meta-analysis to
elucidate whether there were differences in circulating levels of neurologically important essential
trace elements (Zn, Fe, Co, Cu, Mn, and Se) between MS cases and controls. (3) Results: This study
revealed significantly lower serum/plasma Zn and Fe levels and higher Cu levels in MS-affected
individuals compared to controls. At the same time, no significant differences were found between
the MS cases and controls regarding their serum/plasma levels of Co, Mn, or Se. Thus, the loss of Fe
and Zn should be considered in supplementation/nutrition strategies for MS patients. On the other
hand, since high serum Cu levels indicate a burden on the bloodstreams of MS patients, Cu should
be excluded from mineral supplement strategies. Furthermore, all three trace elements (Fe, Zn, and
Cu) should be considered from an etiological point of view, and, most importantly, their levels in the
bloodstreams of MS patients should be monitored. (4) Conclusions: This study highlights the way for
personalized and targeted strategies in the management of MS.",
publisher = "Basel: MDPI",
journal = "Biomedicines",
title = "Changes of Target Essential Trace Elements in Multiple Sclerosis: A Systematic Review and Meta-Analysis",
number = "1589",
volume = "12",
doi = "10.3390/biomedicines12071589"
}

Stojsavljević, A., Jagodić, J., Perović, T., Manojlović, D.,& Pavlović, S.. (2024). Changes of Target Essential Trace Elements in Multiple Sclerosis: A Systematic Review and Meta-Analysis. in Biomedicines
Basel: MDPI., 12(1589).
https://doi.org/10.3390/biomedicines12071589

Stojsavljević A, Jagodić J, Perović T, Manojlović D, Pavlović S. Changes of Target Essential Trace Elements in Multiple Sclerosis: A Systematic Review and Meta-Analysis. in Biomedicines. 2024;12(1589).
doi:10.3390/biomedicines12071589 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Perović, Tatjana, Manojlović, Dragan, Pavlović, Slađan, "Changes of Target Essential Trace Elements in Multiple Sclerosis: A Systematic Review and Meta-Analysis" in Biomedicines, 12, no. 1589 (2024),
https://doi.org/10.3390/biomedicines12071589 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Dinčić, Evica
AU  - Kuveljić, Jovana
AU  - Manojlović, Dragan
AU  - Živković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6584
AB  - Background: Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in
trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort
remains elusive.
Methods: This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS
patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our
main objective was to identify potential variations in elemental profiles based on demographic and clinical
parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace
elements.
Results: Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS
patients compared to controls. These trace elements not only discriminated between MS patients and controls but
also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata
(20–40 years and 41–60 years) revealed discernible variations in elemental profiles between MS patients and
their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild
relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly
differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from
PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation
with EDSS.
Conclusion: These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting
targeted supplementation with these trace elements. This study provides a comprehensive understanding of the
intricate relationship between essential trace elements and MS, paving the way for further research into
personalized nutritional interventions for this complex neurological disorder.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation
VL  - 83
DO  - 10.1016/j.jtemb.2024.127421
SP  - 127421
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Pavlović, Slađan and Dinčić, Evica and Kuveljić, Jovana and Manojlović, Dragan and Živković, Maja",
year = "2024",
abstract = "Background: Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in
trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort
remains elusive.
Methods: This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS
patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our
main objective was to identify potential variations in elemental profiles based on demographic and clinical
parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace
elements.
Results: Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS
patients compared to controls. These trace elements not only discriminated between MS patients and controls but
also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata
(20–40 years and 41–60 years) revealed discernible variations in elemental profiles between MS patients and
their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild
relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly
differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from
PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation
with EDSS.
Conclusion: These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting
targeted supplementation with these trace elements. This study provides a comprehensive understanding of the
intricate relationship between essential trace elements and MS, paving the way for further research into
personalized nutritional interventions for this complex neurological disorder.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation",
volume = "83",
doi = "10.1016/j.jtemb.2024.127421",
pages = "127421"
}

Stojsavljević, A., Jagodić, J., Pavlović, S., Dinčić, E., Kuveljić, J., Manojlović, D.,& Živković, M.. (2024). Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation. in Journal of Trace Elements in Medicine and Biology
Elsevier., 83, 127421.
https://doi.org/10.1016/j.jtemb.2024.127421

Stojsavljević A, Jagodić J, Pavlović S, Dinčić E, Kuveljić J, Manojlović D, Živković M. Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation. in Journal of Trace Elements in Medicine and Biology. 2024;83:127421.
doi:10.1016/j.jtemb.2024.127421 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Pavlović, Slađan, Dinčić, Evica, Kuveljić, Jovana, Manojlović, Dragan, Živković, Maja, "Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation" in Journal of Trace Elements in Medicine and Biology, 83 (2024):127421,
https://doi.org/10.1016/j.jtemb.2024.127421 . .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Stefanović, Radomir
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6840
AB  - To compare the effects of organic selenium nanoparticles (SeNPs, Se0) and inorganic
sodium selenite (NaSe, Na2SeO3, Se4+) on the antioxidant response in maternal and fetal rat liver,
pregnant females were treated with two forms of selenium (Se) at equivalent doses during gestation
(0.5 mg SeNPs or 0.5 mg NaSe/kg body weight/day). Structural parameters of the liver of gravid
females and their fetuses were examined in a sex-specific manner. The oxidative stress parameters
superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase
(GR), glutathione S-transferase (GST), total glutathione (GSH) and sulfhydryl groups (SH) were
established. In addition, the Se concentration was determined in the blood, liver, urine and feces
of the gravid females and in the liver of the fetuses. The structure of the liver of gravid females
remained histologically the same after supplementation with both forms of Se, while the oxidative
stress in the liver was significantly lower after the use of SeNPs compared to NaSe. Immaturity of
fetal antioxidant defenses and sex specificity were demonstrated. This study provides a detailed
insight into the differences in the bioavailability of the nano form of Se compared to sodium selenite
in the livers of pregnant females and fetuses.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Antioxidant Response of Maternal and Fetal Rat Liver to Selenium Nanoparticle Supplementation Compared to Sodium Selenite: Sex Differences between Fetuses
IS  - 7
VL  - 13
DO  - 10.3390/antiox13070756
SP  - 756
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Stefanović, Radomir and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2024",
abstract = "To compare the effects of organic selenium nanoparticles (SeNPs, Se0) and inorganic
sodium selenite (NaSe, Na2SeO3, Se4+) on the antioxidant response in maternal and fetal rat liver,
pregnant females were treated with two forms of selenium (Se) at equivalent doses during gestation
(0.5 mg SeNPs or 0.5 mg NaSe/kg body weight/day). Structural parameters of the liver of gravid
females and their fetuses were examined in a sex-specific manner. The oxidative stress parameters
superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase
(GR), glutathione S-transferase (GST), total glutathione (GSH) and sulfhydryl groups (SH) were
established. In addition, the Se concentration was determined in the blood, liver, urine and feces
of the gravid females and in the liver of the fetuses. The structure of the liver of gravid females
remained histologically the same after supplementation with both forms of Se, while the oxidative
stress in the liver was significantly lower after the use of SeNPs compared to NaSe. Immaturity of
fetal antioxidant defenses and sex specificity were demonstrated. This study provides a detailed
insight into the differences in the bioavailability of the nano form of Se compared to sodium selenite
in the livers of pregnant females and fetuses.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Antioxidant Response of Maternal and Fetal Rat Liver to Selenium Nanoparticle Supplementation Compared to Sodium Selenite: Sex Differences between Fetuses",
number = "7",
volume = "13",
doi = "10.3390/antiox13070756",
pages = "756"
}

Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Stefanović, R., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2024). Antioxidant Response of Maternal and Fetal Rat Liver to Selenium Nanoparticle Supplementation Compared to Sodium Selenite: Sex Differences between Fetuses. in Antioxidants
Basel: MDPI., 13(7), 756.
https://doi.org/10.3390/antiox13070756

Manojlović-Stojanoski M, Borković-Mitić S, Nestorović N, Ristić N, Stefanović R, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. Antioxidant Response of Maternal and Fetal Rat Liver to Selenium Nanoparticle Supplementation Compared to Sodium Selenite: Sex Differences between Fetuses. in Antioxidants. 2024;13(7):756.
doi:10.3390/antiox13070756 .

Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Stefanović, Radomir, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "Antioxidant Response of Maternal and Fetal Rat Liver to Selenium Nanoparticle Supplementation Compared to Sodium Selenite: Sex Differences between Fetuses" in Antioxidants, 13, no. 7 (2024):756,
https://doi.org/10.3390/antiox13070756 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Vlahović, Dragana
AU  - Miler, Marko
AU  - Cesar, Thais
AU  - Ajdžanović, Vladimir
AU  - Milenković, Dragan
AU  - Stellaard, Frans
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Dieter, Lutjohann
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6702
AB  - This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats
IS  - 10
VL  - 25
DO  - 10.3390/ijms25105221
SP  - 5221
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Borković-Mitić, Slavica and Pavlović, Slađan and Vlahović, Dragana and Miler, Marko and Cesar, Thais and Ajdžanović, Vladimir and Milenković, Dragan and Stellaard, Frans and Trifunović, Svetlana and Filipović, Branko and Dieter, Lutjohann",
year = "2024",
abstract = "This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats",
number = "10",
volume = "25",
doi = "10.3390/ijms25105221",
pages = "5221"
}

Šošić-Jurjević, B., Borković-Mitić, S., Pavlović, S., Vlahović, D., Miler, M., Cesar, T., Ajdžanović, V., Milenković, D., Stellaard, F., Trifunović, S., Filipović, B.,& Dieter, L.. (2024). Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences
Basel: MDPI., 25(10), 5221.
https://doi.org/10.3390/ijms25105221

Šošić-Jurjević B, Borković-Mitić S, Pavlović S, Vlahović D, Miler M, Cesar T, Ajdžanović V, Milenković D, Stellaard F, Trifunović S, Filipović B, Dieter L. Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences. 2024;25(10):5221.
doi:10.3390/ijms25105221 .

Šošić-Jurjević, Branka, Borković-Mitić, Slavica, Pavlović, Slađan, Vlahović, Dragana, Miler, Marko, Cesar, Thais, Ajdžanović, Vladimir, Milenković, Dragan, Stellaard, Frans, Trifunović, Svetlana, Filipović, Branko, Dieter, Lutjohann, "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats" in International Journal of Molecular Sciences, 25, no. 10 (2024):5221,
https://doi.org/10.3390/ijms25105221 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Trifunović, Svetlana
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Jovanović, Ljubiša
AU  - Ristić, Nataša
AU  - Ljiljana, Marina
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6120
AB  - Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age
IS  - 16
VL  - 24
DO  - 10.3390/ijms241612624
SP  - 12624
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Trifunović, Svetlana and Pavlović, Slađan and Borković-Mitić, Slavica and Jovanović, Ljubiša and Ristić, Nataša and Ljiljana, Marina and Ajdžanović, Vladimir and Filipović, Branko",
year = "2023",
abstract = "Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age",
number = "16",
volume = "24",
doi = "10.3390/ijms241612624",
pages = "12624"
}

Šošić-Jurjević, B., Lütjohann, D., Trifunović, S., Pavlović, S., Borković-Mitić, S., Jovanović, L., Ristić, N., Ljiljana, M., Ajdžanović, V.,& Filipović, B.. (2023). Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences
Basel: MDPI., 24(16), 12624.
https://doi.org/10.3390/ijms241612624

Šošić-Jurjević B, Lütjohann D, Trifunović S, Pavlović S, Borković-Mitić S, Jovanović L, Ristić N, Ljiljana M, Ajdžanović V, Filipović B. Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences. 2023;24(16):12624.
doi:10.3390/ijms241612624 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Trifunović, Svetlana, Pavlović, Slađan, Borković-Mitić, Slavica, Jovanović, Ljubiša, Ristić, Nataša, Ljiljana, Marina, Ajdžanović, Vladimir, Filipović, Branko, "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age" in International Journal of Molecular Sciences, 24, no. 16 (2023):12624,
https://doi.org/10.3390/ijms241612624 . .

TY  - JOUR
AU  - Mitić, Bojan
AU  - Borković-Mitić, Slavica
AU  - Vranković, Jelena
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5658
AB  - The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR),
and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl (SH) groups and glutathione (GSH) were
analyzed in five age classes of the Mediterranean centipede Scolopendra cingulata as follows: embryo, adolescens, maturus
junior, maturus, and maturus senior. The data obtained showed the presence of SOD, CAT, GSH-Px, GR, GST, and SH
groups in embryos. The transition from embryo to adolescens was accompanied by an increase in the activities of all studied
enzymes, in response to the increased production of ROS due to the increased metabolic activity of the centipede associated with growth and development. Our results show that trends in antioxidant enzyme (AOE) activities were not uniform
among adult age classes, suggesting that maturus junior, maturus, and maturus senior differentially respond and/or have
different susceptibility to ROS. On the other hand, GSH concentration in embryos was undetectable, highest in adolescens
and decreased in the latter part of life. Pearson correlation analysis in embryos showed that the activities of the AOEs were
strongly and positively correlated with each other but negatively correlated with GSH and SH groups. At later age classes,
SOD, CAT, GSH-Px, GR, GSH, and SH groups were no longer significantly correlated with GST. In the discriminant analysis,
the variables that separated the age classes were GR, GST, SH groups, and body length. Body length was directly related to
the age of individuals, clearly indicating that development/aging affects the regulation of antioxidant defense in this species.
PB  - Heidelberg: Springer
T2  - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
T1  - Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda)
DO  - 10.1007/s00360-023-01481-w
ER  - 

@article{
author = "Mitić, Bojan and Borković-Mitić, Slavica and Vranković, Jelena and Stojanović, Dalibor and Pavlović, Slađan",
year = "2023",
abstract = "The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR),
and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl (SH) groups and glutathione (GSH) were
analyzed in five age classes of the Mediterranean centipede Scolopendra cingulata as follows: embryo, adolescens, maturus
junior, maturus, and maturus senior. The data obtained showed the presence of SOD, CAT, GSH-Px, GR, GST, and SH
groups in embryos. The transition from embryo to adolescens was accompanied by an increase in the activities of all studied
enzymes, in response to the increased production of ROS due to the increased metabolic activity of the centipede associated with growth and development. Our results show that trends in antioxidant enzyme (AOE) activities were not uniform
among adult age classes, suggesting that maturus junior, maturus, and maturus senior differentially respond and/or have
different susceptibility to ROS. On the other hand, GSH concentration in embryos was undetectable, highest in adolescens
and decreased in the latter part of life. Pearson correlation analysis in embryos showed that the activities of the AOEs were
strongly and positively correlated with each other but negatively correlated with GSH and SH groups. At later age classes,
SOD, CAT, GSH-Px, GR, GSH, and SH groups were no longer significantly correlated with GST. In the discriminant analysis,
the variables that separated the age classes were GR, GST, SH groups, and body length. Body length was directly related to
the age of individuals, clearly indicating that development/aging affects the regulation of antioxidant defense in this species.",
publisher = "Heidelberg: Springer",
journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",
title = "Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda)",
doi = "10.1007/s00360-023-01481-w"
}

Mitić, B., Borković-Mitić, S., Vranković, J., Stojanović, D.,& Pavlović, S.. (2023). Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda). in Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Heidelberg: Springer..
https://doi.org/10.1007/s00360-023-01481-w

Mitić B, Borković-Mitić S, Vranković J, Stojanović D, Pavlović S. Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda). in Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology. 2023;.
doi:10.1007/s00360-023-01481-w .

Mitić, Bojan, Borković-Mitić, Slavica, Vranković, Jelena, Stojanović, Dalibor, Pavlović, Slađan, "Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda)" in Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology (2023),
https://doi.org/10.1007/s00360-023-01481-w . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Lakićević, Novak
AU  - Pavlović, Slađan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6122
AB  - Environmental pollutants, particularly toxic trace metals with neurotoxic potential, have
been related to the genesis of autism. One of these metals that stands out, in particular, is lead (Pb).
We conducted an in-depth systematic review and meta-analysis of peer-reviewed studies on Pb levels
in biological materials retrieved from autistic children (cases) and neurotypical children (controls)
in this work. A systematic review was conducted after the careful selection of published studies
according to established criteria to gain a broad insight into the higher or lower levels of Pb in the
biological materials of cases and controls, and the findings were then strengthened by a meta-analysis.
The meta-analysis included 17 studies (hair), 13 studies (whole blood), and 8 studies (urine). The
overall number of controls/cases was 869/915 (hair), 670/755 (whole blood), and 344/373 (urine).
This meta-analysis showed significantly higher Pb levels in all three types of biological material in
cases than in controls, suggesting a higher body Pb burden in autistic children. Thus, environmental
Pb exposure could be related to the genesis of autism. Since no level of Pb can be considered safe,
the data from this study undoubtedly point to the importance of regularly monitoring Pb levels in
autistic children.
PB  - Basel: MDPI
T2  - Toxics
T1  - Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis
IS  - 9
VL  - 11
DO  - 10.3390/toxics11090753
SP  - 753
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Lakićević, Novak and Pavlović, Slađan",
year = "2023",
abstract = "Environmental pollutants, particularly toxic trace metals with neurotoxic potential, have
been related to the genesis of autism. One of these metals that stands out, in particular, is lead (Pb).
We conducted an in-depth systematic review and meta-analysis of peer-reviewed studies on Pb levels
in biological materials retrieved from autistic children (cases) and neurotypical children (controls)
in this work. A systematic review was conducted after the careful selection of published studies
according to established criteria to gain a broad insight into the higher or lower levels of Pb in the
biological materials of cases and controls, and the findings were then strengthened by a meta-analysis.
The meta-analysis included 17 studies (hair), 13 studies (whole blood), and 8 studies (urine). The
overall number of controls/cases was 869/915 (hair), 670/755 (whole blood), and 344/373 (urine).
This meta-analysis showed significantly higher Pb levels in all three types of biological material in
cases than in controls, suggesting a higher body Pb burden in autistic children. Thus, environmental
Pb exposure could be related to the genesis of autism. Since no level of Pb can be considered safe,
the data from this study undoubtedly point to the importance of regularly monitoring Pb levels in
autistic children.",
publisher = "Basel: MDPI",
journal = "Toxics",
title = "Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis",
number = "9",
volume = "11",
doi = "10.3390/toxics11090753",
pages = "753"
}

Stojsavljević, A., Lakićević, N.,& Pavlović, S.. (2023). Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis. in Toxics
Basel: MDPI., 11(9), 753.
https://doi.org/10.3390/toxics11090753

Stojsavljević A, Lakićević N, Pavlović S. Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis. in Toxics. 2023;11(9):753.
doi:10.3390/toxics11090753 .

Stojsavljević, Aleksandar, Lakićević, Novak, Pavlović, Slađan, "Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis" in Toxics, 11, no. 9 (2023):753,
https://doi.org/10.3390/toxics11090753 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Lakićević, Novak
AU  - Pavlović, Slađan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6438
AB  - Mercury (Hg) is a non-essential trace metal with unique neurochemical properties and
harmful effects on the central nervous system. In this study, we present a comprehensive review
and meta-analysis of peer-reviewed research encompassing five crucial clinical matrices: hair, whole
blood, plasma, red blood cells (RBCs), and urine. We assess the disparities in Hg levels between
gender- and age-matched neurotypical children (controls) and children diagnosed with autism spectrum disorder (ASD) (cases). After applying rigorous selection criteria, we incorporated a total of
60 case-control studies into our meta-analysis. These studies comprised 25 investigations of Hg levels
in hair (controls/cases: 1134/1361), 15 in whole blood (controls/cases: 1019/1345), 6 in plasma (controls/cases: 224/263), 5 in RBCs (controls/cases: 215/293), and 9 in urine (controls/cases: 399/623).
This meta-analysis did not include the data of ASD children who received chelation therapy. Our
meta-analysis revealed no statistically significant differences in Hg levels in hair and urine between
ASD cases and controls. In whole blood, plasma, and RBCs, Hg levels were significantly higher
in ASD cases compared to their neurotypical counterparts. This indicates that ASD children could
exhibit reduced detoxification capacity for Hg and impaired mechanisms for Hg excretion from their
bodies. This underscores the detrimental role of Hg in ASD and underscores the critical importance of
monitoring Hg levels in ASD children, particularly in early childhood. These findings emphasize the
pressing need for global initiatives aimed at minimizing Hg exposure, thus highlighting the critical
intersection of human–environment interaction and neurodevelopment health.
PB  - Basel: MDPI
T2  - Biomedicines
T1  - Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis
IS  - 3344
IS  - 12
VL  - 11
DO  - 10.3390/biomedicines11123344
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Lakićević, Novak and Pavlović, Slađan",
year = "2023",
abstract = "Mercury (Hg) is a non-essential trace metal with unique neurochemical properties and
harmful effects on the central nervous system. In this study, we present a comprehensive review
and meta-analysis of peer-reviewed research encompassing five crucial clinical matrices: hair, whole
blood, plasma, red blood cells (RBCs), and urine. We assess the disparities in Hg levels between
gender- and age-matched neurotypical children (controls) and children diagnosed with autism spectrum disorder (ASD) (cases). After applying rigorous selection criteria, we incorporated a total of
60 case-control studies into our meta-analysis. These studies comprised 25 investigations of Hg levels
in hair (controls/cases: 1134/1361), 15 in whole blood (controls/cases: 1019/1345), 6 in plasma (controls/cases: 224/263), 5 in RBCs (controls/cases: 215/293), and 9 in urine (controls/cases: 399/623).
This meta-analysis did not include the data of ASD children who received chelation therapy. Our
meta-analysis revealed no statistically significant differences in Hg levels in hair and urine between
ASD cases and controls. In whole blood, plasma, and RBCs, Hg levels were significantly higher
in ASD cases compared to their neurotypical counterparts. This indicates that ASD children could
exhibit reduced detoxification capacity for Hg and impaired mechanisms for Hg excretion from their
bodies. This underscores the detrimental role of Hg in ASD and underscores the critical importance of
monitoring Hg levels in ASD children, particularly in early childhood. These findings emphasize the
pressing need for global initiatives aimed at minimizing Hg exposure, thus highlighting the critical
intersection of human–environment interaction and neurodevelopment health.",
publisher = "Basel: MDPI",
journal = "Biomedicines",
title = "Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis",
number = "3344, 12",
volume = "11",
doi = "10.3390/biomedicines11123344"
}

Stojsavljević, A., Lakićević, N.,& Pavlović, S.. (2023). Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis. in Biomedicines
Basel: MDPI., 11(3344).
https://doi.org/10.3390/biomedicines11123344

Stojsavljević A, Lakićević N, Pavlović S. Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis. in Biomedicines. 2023;11(3344).
doi:10.3390/biomedicines11123344 .

Stojsavljević, Aleksandar, Lakićević, Novak, Pavlović, Slađan, "Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis" in Biomedicines, 11, no. 3344 (2023),
https://doi.org/10.3390/biomedicines11123344 . .

TY  - CONF
AU  - Mitić, Bojan
AU  - Vranković, Jelena
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6121
AB  - Sex-specific physiology includes a set of physiological traits that differ markedly
between males and females of the same species. In addition to the most obvious
differences between the sexes, such as reproductive roles controlled by sex-specific
endocrine systems, it has been demonstrated that the sexes may also differ in their
oxidative physiology. Centipedes, like other animals, possess a suite of antioxidant
enzymes (AOEs) and non-enzymatic antioxidants that delay, prevent, or remove
oxidative damage to target molecules. The aim of the present study was to
investigate sex-specific differences in the antioxidant defence system in three
centipede species from the Balkan Peninsula.
Here, we measured the activity of the following AOEs: superoxide dismutase
(SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase
(GR), and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl
(SH) groups and glutathione (GSH) in males and females of Eupolybothrus
transsylvanicus, Cryptops anomalans, and Clinopodes flavidus. In addition, we
measured lipid peroxidation (LPO) and protein carbonyl (PCO) concentrations in the
species studied to investigate whether sex-specific trends in the activity of AOEs may
lead to the occurrence of oxidative damage.
E. transsylvanicus, C. anomalans and C. flavidus were collected by hand from
under stones and bark on Ada Ciganlija Island (Belgrade, Serbia) in October 2022.
The sexes were distinguished by external characteristics or by dissection of genital
appendages. The biochemical and statistical analyses were performed following our
previous work in the field.
Overall, the most significant differences were found between E. transsylvanicus
and C. anomalans in the following parameters: SOD, CAT, GR, SH groups, LPO, and
PCO; then between C. anomalans and C. flavidus (GSH-Px, GR, SH groups, LPO, and
PCO), whereas the least significant differences were between E. transsylvanicus and
C. flavidus (GSH-Px only). Besides, SOD, GSH-Px and GSH showed significant
differences between males and females of E. transsylvanicus. In C. anomalans, GSHPx and GSH were significantly divided by sex, while in C. flavidus this was true only
for GST. Moreover, all measured AOEs in E. transsylvanicus had higher values in
males, while in the other two species the situation was reversed – the studied
parameters were higher in females.
Thus, the level of antioxidant defences depends on the species and sex of the
centipedes studied. Our results also suggest that females of centipedes that exhibit
parental care, such as C. anomalans and C. flavidus, have better antioxidant status
than males. This provides a starting point for future research on the evolutionary
causes of sex specificity in the oxidative physiology of centipedes.
PB  - Bogotá: Universidad Nacional De Colombia
C3  - Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia
T1  - Antioxidant defences in centipedes (Chilopoda): a question of sex
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6121
ER  - 

@conference{
author = "Mitić, Bojan and Vranković, Jelena and Stojanović, Dalibor and Pavlović, Slađan and Borković-Mitić, Slavica",
year = "2023",
abstract = "Sex-specific physiology includes a set of physiological traits that differ markedly
between males and females of the same species. In addition to the most obvious
differences between the sexes, such as reproductive roles controlled by sex-specific
endocrine systems, it has been demonstrated that the sexes may also differ in their
oxidative physiology. Centipedes, like other animals, possess a suite of antioxidant
enzymes (AOEs) and non-enzymatic antioxidants that delay, prevent, or remove
oxidative damage to target molecules. The aim of the present study was to
investigate sex-specific differences in the antioxidant defence system in three
centipede species from the Balkan Peninsula.
Here, we measured the activity of the following AOEs: superoxide dismutase
(SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase
(GR), and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl
(SH) groups and glutathione (GSH) in males and females of Eupolybothrus
transsylvanicus, Cryptops anomalans, and Clinopodes flavidus. In addition, we
measured lipid peroxidation (LPO) and protein carbonyl (PCO) concentrations in the
species studied to investigate whether sex-specific trends in the activity of AOEs may
lead to the occurrence of oxidative damage.
E. transsylvanicus, C. anomalans and C. flavidus were collected by hand from
under stones and bark on Ada Ciganlija Island (Belgrade, Serbia) in October 2022.
The sexes were distinguished by external characteristics or by dissection of genital
appendages. The biochemical and statistical analyses were performed following our
previous work in the field.
Overall, the most significant differences were found between E. transsylvanicus
and C. anomalans in the following parameters: SOD, CAT, GR, SH groups, LPO, and
PCO; then between C. anomalans and C. flavidus (GSH-Px, GR, SH groups, LPO, and
PCO), whereas the least significant differences were between E. transsylvanicus and
C. flavidus (GSH-Px only). Besides, SOD, GSH-Px and GSH showed significant
differences between males and females of E. transsylvanicus. In C. anomalans, GSHPx and GSH were significantly divided by sex, while in C. flavidus this was true only
for GST. Moreover, all measured AOEs in E. transsylvanicus had higher values in
males, while in the other two species the situation was reversed – the studied
parameters were higher in females.
Thus, the level of antioxidant defences depends on the species and sex of the
centipedes studied. Our results also suggest that females of centipedes that exhibit
parental care, such as C. anomalans and C. flavidus, have better antioxidant status
than males. This provides a starting point for future research on the evolutionary
causes of sex specificity in the oxidative physiology of centipedes.",
publisher = "Bogotá: Universidad Nacional De Colombia",
journal = "Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia",
title = "Antioxidant defences in centipedes (Chilopoda): a question of sex",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6121"
}

Mitić, B., Vranković, J., Stojanović, D., Pavlović, S.,& Borković-Mitić, S.. (2023). Antioxidant defences in centipedes (Chilopoda): a question of sex. in Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia
Bogotá: Universidad Nacional De Colombia..
https://hdl.handle.net/21.15107/rcub_ibiss_6121

Mitić B, Vranković J, Stojanović D, Pavlović S, Borković-Mitić S. Antioxidant defences in centipedes (Chilopoda): a question of sex. in Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6121 .

Mitić, Bojan, Vranković, Jelena, Stojanović, Dalibor, Pavlović, Slađan, Borković-Mitić, Slavica, "Antioxidant defences in centipedes (Chilopoda): a question of sex" in Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6121 .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5198
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta
IS  - 21
VL  - 23
DO  - 10.3390/ijms232113068
SP  - 13068
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Borković Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta",
number = "21",
volume = "23",
doi = "10.3390/ijms232113068",
pages = "13068"
}

Manojlović-Stojanoski, M., Borković Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences
Basel: MDPI., 23(21), 13068.
https://doi.org/10.3390/ijms232113068

Manojlović-Stojanoski M, Borković Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences. 2022;23(21):13068.
doi:10.3390/ijms232113068 .

Manojlović-Stojanoski, Milica, Borković Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://doi.org/10.3390/ijms232113068 . .

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Perović, Milan
AU  - Miković, Željko
AU  - Đurđić, Slađana
AU  - Manojlović, Dragan
AU  - Stojsavljević, Aleksandar
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5020
AB  - With the ever-growing concern for human health and wellbeing, the prenatal period of
development requires special attention since fetuses can be exposed to various metals through the
mother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)
and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, and
placental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on the
potential transplacental transfer of these trace metals. Based on the obtained levels of investigated
elements in clinical samples, it was observed that all of the trace metals cross the placental barrier
and reach the fetus. Furthermore, statistical analysis revealed significant differences in levels of
toxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinical
samples. Correlation analysis highlighted As to be an element with levels that differed significantly
between all tested samples. Principal component analysis (PCA) was used to enhance these findings.
PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals in
distinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible for
the clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera was
responsible for the clustering of these samples. Overall, the findings of this study could contribute to
a better understanding of transplacental transfer of these trace metals, and shed a light on overall
levels of metal exposure in the population of healthy pregnant women and their fetuses.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy
IS  - 15
VL  - 23
DO  - 10.3390/ijms23158078
SP  - 8078
ER  - 

@article{
author = "Jagodić, Jovana and Pavlović, Slađan and Borković Mitić, Slavica and Perović, Milan and Miković, Željko and Đurđić, Slađana and Manojlović, Dragan and Stojsavljević, Aleksandar",
year = "2022",
abstract = "With the ever-growing concern for human health and wellbeing, the prenatal period of
development requires special attention since fetuses can be exposed to various metals through the
mother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)
and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, and
placental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on the
potential transplacental transfer of these trace metals. Based on the obtained levels of investigated
elements in clinical samples, it was observed that all of the trace metals cross the placental barrier
and reach the fetus. Furthermore, statistical analysis revealed significant differences in levels of
toxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinical
samples. Correlation analysis highlighted As to be an element with levels that differed significantly
between all tested samples. Principal component analysis (PCA) was used to enhance these findings.
PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals in
distinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible for
the clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera was
responsible for the clustering of these samples. Overall, the findings of this study could contribute to
a better understanding of transplacental transfer of these trace metals, and shed a light on overall
levels of metal exposure in the population of healthy pregnant women and their fetuses.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy",
number = "15",
volume = "23",
doi = "10.3390/ijms23158078",
pages = "8078"
}

Jagodić, J., Pavlović, S., Borković Mitić, S., Perović, M., Miković, Ž., Đurđić, S., Manojlović, D.,& Stojsavljević, A.. (2022). Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences
Basel: MDPI., 23(15), 8078.
https://doi.org/10.3390/ijms23158078

Jagodić J, Pavlović S, Borković Mitić S, Perović M, Miković Ž, Đurđić S, Manojlović D, Stojsavljević A. Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences. 2022;23(15):8078.
doi:10.3390/ijms23158078 .

Jagodić, Jovana, Pavlović, Slađan, Borković Mitić, Slavica, Perović, Milan, Miković, Željko, Đurđić, Slađana, Manojlović, Dragan, Stojsavljević, Aleksandar, "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy" in International Journal of Molecular Sciences, 23, no. 15 (2022):8078,
https://doi.org/10.3390/ijms23158078 . .

TY  - CONF
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5319
AB  - Селен (Se) је металоид неопходан за правилно функционисање организма. Улази у
састав више од 25 селенопротеина. Неопходан је за антиоксидативни систем
(АОС), метаболизам тиреоидних хормона, синтезу ДНК, плодност, имунитет…
Концентрације Se у организму варирају у малом опсегу од чега зависи његов
дефицит, оптималан биолошки учинак или токсичност.
1 Селен продире у
организам у неорганском (селенит или селенат) и органском (селенометионин и
селеноцистеин) облику. Органски облици селена показују најбољу
биорасположивост.2
Биомедицински ефекти честица нано селена (SeNPs) су
интензивно истраживани током протекле деценије.3 Циљ ове студије био је да се
упореде ефекти SeNPs и Na-селенита на АОС у плаценти гравидних женки пацова.
Плацента је посредник између мајке и фетуса и учествује у транспорту Se током
целог интраутериног развоја. Гравидне женке Wistar albino пацова су гаважом
свакога дана добијале SeNPs или Na-селенит. Испитивана је активност супероксид
дисмутазе (SOD), каталазе (CAT), глутатион пероксидазе (GSH-Px), глутатион
редуктазе (GR) и глутатион С-трансферазе (GST), као и концентрације глутатиона
(GSH) и сулфхидрилних група (SH). SeNPs је довео до значајног повећања
активности SOD, CAT и GSH-Px и смањења активности GST и концентрација GSH
и SH у плаценти, док је третман Na-селенитом довео до повећања концентрације
GSH у односу на контроле. Добијени резултати указују да SeNPs и Na-селенит
изазивају различит физиолошки ефекат у плаценти гравидних женки пацова, а
даља истраживања треба да утврде да ли је у питању повећано оксидативно
оптерећење или побољшана антиоксидативна заштита.
AB  - Selen (Se) je metaloid neophodan za pravilno funkcionisanje organizma. Ulazi u sastav više od 25 selenoproteina. Neophodan je za antioksidativni sistem (AOS), metabolizam tireoidnih hormona, sintezu DNK, plodnost, imunitet… Koncentracije Se u organizmu variraju u malom opsegu od čega zavisi njegov deficit, optimalan biološki učinak ili toksičnost. 1 Selen prodire u organizam u neorganskom (selenit ili selenat) i organskom (selenometionin i selenocistein) obliku. Organski oblici selena pokazuju najbolju bioraspoloživost.2 Biomedicinski efekti čestica nano selena (SeNPs) su intenzivno istraživani tokom protekle decenije.3 Cilj ove studije bio je da se uporede efekti SeNPs i Na-selenita na AOS u placenti gravidnih ženki pacova. Placenta je posrednik između majke i fetusa i učestvuje u transportu Se tokom celog intrauterinog razvoja. Gravidne ženke Wistar albino pacova su gavažom svakoga dana dobijale SeNPs ili Na-selenit. Ispitivana je aktivnost superoksid dismutaze (SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation S-transferaze (GST), kao i koncentracije glutationa (GSH) i sulfhidrilnih grupa (SH). SeNPs je doveo do značajnog povećanja aktivnosti SOD, CAT i GSH-Px i smanjenja aktivnosti GST i koncentracija GSH i SH u placenti, dok je tretman Na-selenitom doveo do povećanja koncentracije GSH u odnosu na kontrole. Dobijeni rezultati ukazuju da SeNPs i Na-selenit izazivaju različit fiziološki efekat u placenti gravidnih ženki pacova, a dalja istraživanja treba da utvrde da li je u pitanju povećano oksidativno opterećenje ili poboljšana antioksidativna zaštita.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti različitih formi selena na na antioksidativni status placente pacova
T1  - Ефекти различитих форми селена на антиоксидативни статус плаценте пацова
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5319
ER  - 

@conference{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Ristić, Nataša and Nestorović, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Pavlović, Slađan",
year = "2022",
abstract = "Селен (Se) је металоид неопходан за правилно функционисање организма. Улази у
састав више од 25 селенопротеина. Неопходан је за антиоксидативни систем
(АОС), метаболизам тиреоидних хормона, синтезу ДНК, плодност, имунитет…
Концентрације Se у организму варирају у малом опсегу од чега зависи његов
дефицит, оптималан биолошки учинак или токсичност.
1 Селен продире у
организам у неорганском (селенит или селенат) и органском (селенометионин и
селеноцистеин) облику. Органски облици селена показују најбољу
биорасположивост.2
Биомедицински ефекти честица нано селена (SeNPs) су
интензивно истраживани током протекле деценије.3 Циљ ове студије био је да се
упореде ефекти SeNPs и Na-селенита на АОС у плаценти гравидних женки пацова.
Плацента је посредник између мајке и фетуса и учествује у транспорту Se током
целог интраутериног развоја. Гравидне женке Wistar albino пацова су гаважом
свакога дана добијале SeNPs или Na-селенит. Испитивана је активност супероксид
дисмутазе (SOD), каталазе (CAT), глутатион пероксидазе (GSH-Px), глутатион
редуктазе (GR) и глутатион С-трансферазе (GST), као и концентрације глутатиона
(GSH) и сулфхидрилних група (SH). SeNPs је довео до значајног повећања
активности SOD, CAT и GSH-Px и смањења активности GST и концентрација GSH
и SH у плаценти, док је третман Na-селенитом довео до повећања концентрације
GSH у односу на контроле. Добијени резултати указују да SeNPs и Na-селенит
изазивају различит физиолошки ефекат у плаценти гравидних женки пацова, а
даља истраживања треба да утврде да ли је у питању повећано оксидативно
оптерећење или побољшана антиоксидативна заштита., Selen (Se) je metaloid neophodan za pravilno funkcionisanje organizma. Ulazi u sastav više od 25 selenoproteina. Neophodan je za antioksidativni sistem (AOS), metabolizam tireoidnih hormona, sintezu DNK, plodnost, imunitet… Koncentracije Se u organizmu variraju u malom opsegu od čega zavisi njegov deficit, optimalan biološki učinak ili toksičnost. 1 Selen prodire u organizam u neorganskom (selenit ili selenat) i organskom (selenometionin i selenocistein) obliku. Organski oblici selena pokazuju najbolju bioraspoloživost.2 Biomedicinski efekti čestica nano selena (SeNPs) su intenzivno istraživani tokom protekle decenije.3 Cilj ove studije bio je da se uporede efekti SeNPs i Na-selenita na AOS u placenti gravidnih ženki pacova. Placenta je posrednik između majke i fetusa i učestvuje u transportu Se tokom celog intrauterinog razvoja. Gravidne ženke Wistar albino pacova su gavažom svakoga dana dobijale SeNPs ili Na-selenit. Ispitivana je aktivnost superoksid dismutaze (SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation S-transferaze (GST), kao i koncentracije glutationa (GSH) i sulfhidrilnih grupa (SH). SeNPs je doveo do značajnog povećanja aktivnosti SOD, CAT i GSH-Px i smanjenja aktivnosti GST i koncentracija GSH i SH u placenti, dok je tretman Na-selenitom doveo do povećanja koncentracije GSH u odnosu na kontrole. Dobijeni rezultati ukazuju da SeNPs i Na-selenit izazivaju različit fiziološki efekat u placenti gravidnih ženki pacova, a dalja istraživanja treba da utvrde da li je u pitanju povećano oksidativno opterećenje ili poboljšana antioksidativna zaštita.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti različitih formi selena na na antioksidativni status placente pacova, Ефекти различитих форми селена на антиоксидативни статус плаценте пацова",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5319"
}

Manojlović-Stojanoski, M., Borković-Mitić, S., Ristić, N., Nestorović, N., Trifunović, S., Stevanović, M.,& Pavlović, S.. (2022). Efekti različitih formi selena na na antioksidativni status placente pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5319

Manojlović-Stojanoski M, Borković-Mitić S, Ristić N, Nestorović N, Trifunović S, Stevanović M, Pavlović S. Efekti različitih formi selena na na antioksidativni status placente pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5319 .

Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Ristić, Nataša, Nestorović, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Pavlović, Slađan, "Efekti različitih formi selena na na antioksidativni status placente pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5319 .

TY  - CONF
AU  - Lakić, Iva
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Stanković, Sanja
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5933
AB  - Сепса је стање опасно по живот узроковано нерегулисаним и прекомерним
одговором на инфекцију, које праћено инфламацијом и поремећеним
метаболизмом липида доводи до постепеног отказивања органа.1,2 Мелдонијум је
антиинфламаторни лек који се користи за лечење исхемије миокарда.3 Он делује
тако што преусмерава производњу енергије са оксидације масних киселина на
гликолизу, као пут који троши мање кисеоника. На овај начин мелдонијум
негативно утиче на метаболизам липида. Истраживали смо ефекте
четворонедељног претретмана мелдонијумом на ток фекалне сепсе (ФИП) и сепсе
изазиване липополисахаридом (ЛПС), код мужјака пацова. Изненађујуће, у
условима фекалне сепсе, мелдонијум је повећао стопу морталитета животиња у
поређењу са групом која није третирана мелдонијумом. Анализе оксидативног и
инфламаторног статуса ткива (срце, јетра, бубрези) потврдиле су
антиинфламаторне, антиапоптотске и антинекротичке ефекте мелдонијума, код оба
модела сепсе. Када је реч о производњи енергије, упркос неким сличностима,
утврђене су разлике у ова два модела. Једна од очигледних разлика је у одговору
симпатоадреналног система, који је изостао код ФИП модела, док је код ЛПС
модела довео до двоструког повећања концентрације катехоламина у серуму. Ова
студија показује важност ненарушене производње енергије скрећући пажњу на
потребу ревизије постојећих смерница у клиничком лечењу сепсе, али и отвара пут
за откривање нових терапијских приступа.
AB  - Sepsa je stanje opasno po život uzrokovano neregulisanim i prekomernim odgovorom na infekciju, koje praćeno inflamacijom i poremećenim metabolizmom lipida dovodi do postepenog otkazivanja organa.1,2 Meldonijum je antiinflamatorni lek koji se koristi za lečenje ishemije miokarda.3 On deluje tako što preusmerava proizvodnju energije sa oksidacije masnih kiselina na glikolizu, kao put koji troši manje kiseonika. Na ovaj način meldonijum negativno utiče na metabolizam lipida. Istraživali smo efekte četvoronedeljnog pretretmana meldonijumom na tok fekalne sepse (FIP) i sepse izazivane lipopolisaharidom (LPS), kod mužjaka pacova. Iznenađujuće, u uslovima fekalne sepse, meldonijum je povećao stopu mortaliteta životinja u poređenju sa grupom koja nije tretirana meldonijumom. Analize oksidativnog i inflamatornog statusa tkiva (srce, jetra, bubrezi) potvrdile su antiinflamatorne, antiapoptotske i antinekrotičke efekte meldonijuma, kod oba modela sepse. Kada je reč o proizvodnji energije, uprkos nekim sličnostima, utvrđene su razlike u ova dva modela. Jedna od očiglednih razlika je u odgovoru simpatoadrenalnog sistema, koji je izostao kod FIP modela, dok je kod LPS modela doveo do dvostrukog povećanja koncentracije kateholamina u serumu. Ova studija pokazuje važnost nenarušene proizvodnje energije skrećući pažnju na potrebu revizije postojećih smernica u kliničkom lečenju sepse, ali i otvara put za otkrivanje novih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova
T1  - Ефекти претретмана мелдонијумом на токове различитих модела сепси код пацова
SP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5933
ER  - 

@conference{
author = "Lakić, Iva and Đurašević, Siniša and Ružičić, Aleksandra and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pavlović, Slađan and Borković-Mitić, Slavica and Grigorov, Ilijana and Stanković, Sanja and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2022",
abstract = "Сепса је стање опасно по живот узроковано нерегулисаним и прекомерним
одговором на инфекцију, које праћено инфламацијом и поремећеним
метаболизмом липида доводи до постепеног отказивања органа.1,2 Мелдонијум је
антиинфламаторни лек који се користи за лечење исхемије миокарда.3 Он делује
тако што преусмерава производњу енергије са оксидације масних киселина на
гликолизу, као пут који троши мање кисеоника. На овај начин мелдонијум
негативно утиче на метаболизам липида. Истраживали смо ефекте
четворонедељног претретмана мелдонијумом на ток фекалне сепсе (ФИП) и сепсе
изазиване липополисахаридом (ЛПС), код мужјака пацова. Изненађујуће, у
условима фекалне сепсе, мелдонијум је повећао стопу морталитета животиња у
поређењу са групом која није третирана мелдонијумом. Анализе оксидативног и
инфламаторног статуса ткива (срце, јетра, бубрези) потврдиле су
антиинфламаторне, антиапоптотске и антинекротичке ефекте мелдонијума, код оба
модела сепсе. Када је реч о производњи енергије, упркос неким сличностима,
утврђене су разлике у ова два модела. Једна од очигледних разлика је у одговору
симпатоадреналног система, који је изостао код ФИП модела, док је код ЛПС
модела довео до двоструког повећања концентрације катехоламина у серуму. Ова
студија показује важност ненарушене производње енергије скрећући пажњу на
потребу ревизије постојећих смерница у клиничком лечењу сепсе, али и отвара пут
за откривање нових терапијских приступа., Sepsa je stanje opasno po život uzrokovano neregulisanim i prekomernim odgovorom na infekciju, koje praćeno inflamacijom i poremećenim metabolizmom lipida dovodi do postepenog otkazivanja organa.1,2 Meldonijum je antiinflamatorni lek koji se koristi za lečenje ishemije miokarda.3 On deluje tako što preusmerava proizvodnju energije sa oksidacije masnih kiselina na glikolizu, kao put koji troši manje kiseonika. Na ovaj način meldonijum negativno utiče na metabolizam lipida. Istraživali smo efekte četvoronedeljnog pretretmana meldonijumom na tok fekalne sepse (FIP) i sepse izazivane lipopolisaharidom (LPS), kod mužjaka pacova. Iznenađujuće, u uslovima fekalne sepse, meldonijum je povećao stopu mortaliteta životinja u poređenju sa grupom koja nije tretirana meldonijumom. Analize oksidativnog i inflamatornog statusa tkiva (srce, jetra, bubrezi) potvrdile su antiinflamatorne, antiapoptotske i antinekrotičke efekte meldonijuma, kod oba modela sepse. Kada je reč o proizvodnji energije, uprkos nekim sličnostima, utvrđene su razlike u ova dva modela. Jedna od očiglednih razlika je u odgovoru simpatoadrenalnog sistema, koji je izostao kod FIP modela, dok je kod LPS modela doveo do dvostrukog povećanja koncentracije kateholamina u serumu. Ova studija pokazuje važnost nenarušene proizvodnje energije skrećući pažnju na potrebu revizije postojećih smernica u kliničkom lečenju sepse, ali i otvara put za otkrivanje novih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova, Ефекти претретмана мелдонијумом на токове различитих модела сепси код пацова",
pages = "340",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5933"
}

Lakić, I., Đurašević, S., Ružičić, A., Tosti, T., Đurović, S., Glumac, S., Pavlović, S., Borković-Mitić, S., Grigorov, I., Stanković, S., Pejić, S., Todorović, A., Drakulić, D., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2022). Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 340.
https://hdl.handle.net/21.15107/rcub_ibiss_5933

Lakić I, Đurašević S, Ružičić A, Tosti T, Đurović S, Glumac S, Pavlović S, Borković-Mitić S, Grigorov I, Stanković S, Pejić S, Todorović A, Drakulić D, Jasnić N, Đorđević J, Todorović Z. Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:340.
https://hdl.handle.net/21.15107/rcub_ibiss_5933 .

Lakić, Iva, Đurašević, Siniša, Ružičić, Aleksandra, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pavlović, Slađan, Borković-Mitić, Slavica, Grigorov, Ilijana, Stanković, Sanja, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):340,
https://hdl.handle.net/21.15107/rcub_ibiss_5933 .

TY  - CONF
AU  - Borković-Mitić, Slavica
AU  - Mitić, Bojan
AU  - Vranković, Jelena
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5932
AB  - Антиоксидативни ензими су важан механизам одбране од реактивних врста
кисеоника (ROS) и, као и многи други биохемијски системи, њихова ефикасност
може да варира у зависности од фазе развића и других физиолошких аспеката
организма. У овој студији испитивали смо промене у антиоксидативној одбрани
током развића медитеранске врсте стоноге Scolopendra cingulata (Chilopoda). Попут
других бескичмењака који живе у аеробним условима, и ова стонога поседује
кључне антиоксидативне ензиме – супероксид дисмутазу (SOD), каталазу (CAT),
глутатион пероксидазу (GSH-Px), глутатион редуктазу (GR) и глутатион Sтрансферазу (GST). Активност ових ензима је била најнижа у ембрионалној фази, а
њено значајно повећање током ране постембриогенезе учинило је слободноживеће
јединке (стадијум адолесценс) спремнијим за суочавање са условима у којима
настаје већа количина ROS. Наши резултати су показали да промена активности
антиоксидативних ензима, приликом преласка из јувенилне у адултну фазу
(матурус јуниор, матурус и матурус сениор), је вероватно последица бројних
развојних процеса интезивираних у том стадијуму. Важно је да је поред
физиолошких и фактора средине, онтогенетски развој такође одлучујући фактор
нивоа антиоксидативних ензима код ових животиња.
AB  - Antioksidativni enzimi su važan mehanizam odbrane od reaktivnih vrsta kiseonika (ROS) i, kao i mnogi drugi biohemijski sistemi, njihova efikasnost može da varira u zavisnosti od faze razvića i drugih fizioloških aspekata organizma. U ovoj studiji ispitivali smo promene u antioksidativnoj odbrani tokom razvića mediteranske vrste stonoge Scolopendra cingulata (Chilopoda). Poput drugih beskičmenjaka koji žive u aerobnim uslovima, i ova stonoga poseduje ključne antioksidativne enzime – superoksid dismutazu (SOD), katalazu (CAT), glutation peroksidazu (GSH-Px), glutation reduktazu (GR) i glutation Stransferazu (GST). Aktivnost ovih enzima je bila najniža u embrionalnoj fazi, a njeno značajno povećanje tokom rane postembriogeneze učinilo je slobodnoživeće jedinke (stadijum adolescens) spremnijim za suočavanje sa uslovima u kojima nastaje veća količina ROS. Naši rezultati su pokazali da promena aktivnosti antioksidativnih enzima, prilikom prelaska iz juvenilne u adultnu fazu (maturus junior, maturus i maturus senior), je verovatno posledica brojnih razvojnih procesa inteziviranih u tom stadijumu. Važno je da je pored fizioloških i faktora sredine, ontogenetski razvoj takođe odlučujući faktor nivoa antioksidativnih enzima kod ovih životinja.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda)
T1  - Promene aktivnosti antioksidativnog sistema tokom razvića stonoge Scolopendra cingulata (Chilopoda)
SP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5932
ER  - 

@conference{
author = "Borković-Mitić, Slavica and Mitić, Bojan and Vranković, Jelena and Stojanović, Dalibor and Pavlović, Slađan",
year = "2022",
abstract = "Антиоксидативни ензими су важан механизам одбране од реактивних врста
кисеоника (ROS) и, као и многи други биохемијски системи, њихова ефикасност
може да варира у зависности од фазе развића и других физиолошких аспеката
организма. У овој студији испитивали смо промене у антиоксидативној одбрани
током развића медитеранске врсте стоноге Scolopendra cingulata (Chilopoda). Попут
других бескичмењака који живе у аеробним условима, и ова стонога поседује
кључне антиоксидативне ензиме – супероксид дисмутазу (SOD), каталазу (CAT),
глутатион пероксидазу (GSH-Px), глутатион редуктазу (GR) и глутатион Sтрансферазу (GST). Активност ових ензима је била најнижа у ембрионалној фази, а
њено значајно повећање током ране постембриогенезе учинило је слободноживеће
јединке (стадијум адолесценс) спремнијим за суочавање са условима у којима
настаје већа количина ROS. Наши резултати су показали да промена активности
антиоксидативних ензима, приликом преласка из јувенилне у адултну фазу
(матурус јуниор, матурус и матурус сениор), је вероватно последица бројних
развојних процеса интезивираних у том стадијуму. Важно је да је поред
физиолошких и фактора средине, онтогенетски развој такође одлучујући фактор
нивоа антиоксидативних ензима код ових животиња., Antioksidativni enzimi su važan mehanizam odbrane od reaktivnih vrsta kiseonika (ROS) i, kao i mnogi drugi biohemijski sistemi, njihova efikasnost može da varira u zavisnosti od faze razvića i drugih fizioloških aspekata organizma. U ovoj studiji ispitivali smo promene u antioksidativnoj odbrani tokom razvića mediteranske vrste stonoge Scolopendra cingulata (Chilopoda). Poput drugih beskičmenjaka koji žive u aerobnim uslovima, i ova stonoga poseduje ključne antioksidativne enzime – superoksid dismutazu (SOD), katalazu (CAT), glutation peroksidazu (GSH-Px), glutation reduktazu (GR) i glutation Stransferazu (GST). Aktivnost ovih enzima je bila najniža u embrionalnoj fazi, a njeno značajno povećanje tokom rane postembriogeneze učinilo je slobodnoživeće jedinke (stadijum adolescens) spremnijim za suočavanje sa uslovima u kojima nastaje veća količina ROS. Naši rezultati su pokazali da promena aktivnosti antioksidativnih enzima, prilikom prelaska iz juvenilne u adultnu fazu (maturus junior, maturus i maturus senior), je verovatno posledica brojnih razvojnih procesa inteziviranih u tom stadijumu. Važno je da je pored fizioloških i faktora sredine, ontogenetski razvoj takođe odlučujući faktor nivoa antioksidativnih enzima kod ovih životinja.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda), Promene aktivnosti antioksidativnog sistema tokom razvića stonoge Scolopendra cingulata (Chilopoda)",
pages = "176",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5932"
}

Borković-Mitić, S., Mitić, B., Vranković, J., Stojanović, D.,& Pavlović, S.. (2022). Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 176.
https://hdl.handle.net/21.15107/rcub_ibiss_5932

Borković-Mitić S, Mitić B, Vranković J, Stojanović D, Pavlović S. Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:176.
https://hdl.handle.net/21.15107/rcub_ibiss_5932 .

Borković-Mitić, Slavica, Mitić, Bojan, Vranković, Jelena, Stojanović, Dalibor, Pavlović, Slađan, "Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):176,
https://hdl.handle.net/21.15107/rcub_ibiss_5932 .

TY  - JOUR
AU  - Mitić, Bojan
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Sojanović, Dalibor
AU  - Pavlović, Slađan
AU  - Vasiljević, Ljubica
AU  - Ristić, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5044
AB  - Three centipede species (Clinopodes flavidus,Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb,Sr,Cd, Tl, Pb and U and metalloids As,Se) in whole animals and soil were measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed species were observed, both in response to the sites and between species. 1n most cases, the trace element concentrations were higher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E. transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus was a good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient in accumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E. transsylvanicus, which accumulated Cd according to the high bioaccwnulation factor (BAF) values. We conclude that the centipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure. Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the route of exposure.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)
IS  - 3
VL  - 74
DO  - 10.2298/ABS220514019M
SP  - 207
EP  - 215
ER  - 

@article{
author = "Mitić, Bojan and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Sojanović, Dalibor and Pavlović, Slađan and Vasiljević, Ljubica and Ristić, Nataša",
year = "2022",
abstract = "Three centipede species (Clinopodes flavidus,Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb,Sr,Cd, Tl, Pb and U and metalloids As,Se) in whole animals and soil were measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed species were observed, both in response to the sites and between species. 1n most cases, the trace element concentrations were higher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E. transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus was a good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient in accumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E. transsylvanicus, which accumulated Cd according to the high bioaccwnulation factor (BAF) values. We conclude that the centipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure. Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the route of exposure.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)",
number = "3",
volume = "74",
doi = "10.2298/ABS220514019M",
pages = "207-215"
}

Mitić, B., Borković Mitić, S., Stojsavljević, A., Sojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and Metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 74(3), 207-215.
https://doi.org/10.2298/ABS220514019M

Mitić B, Borković Mitić S, Stojsavljević A, Sojanović D, Pavlović S, Vasiljević L, Ristić N. Metal and Metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences. 2022;74(3):207-215.
doi:10.2298/ABS220514019M .

Mitić, Bojan, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Sojanović, Dalibor, Pavlović, Slađan, Vasiljević, Ljubica, Ristić, Nataša, "Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)" in Archives of Biological Sciences, 74, no. 3 (2022):207-215,
https://doi.org/10.2298/ABS220514019M . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Sopta, Jelena
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Ivanović, Anđelija
AU  - Jasnić, Nebojša
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4090
AB  - Acute ischemia/reperfusion (I/R) liver injury is a clinical condition challenging to treat. Meldonium
is an anti‑ischemic agent that shifts energy production from fatty acid oxidation to less oxygen‑
consuming glycolysis. Thus, we investigated the effects of a 4‑week meldonium pre‑treatment
(300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats. Our results showed that
meldonium ameliorates I/R‑induced liver inflammation and injury, as confirmed by liver histology,
and by attenuation of serum alanine‑ and aspartate aminotransferase activity, serum and liver
high mobility group box 1 protein expression, and liver expression of Bax/Bcl2, haptoglobin, and
the phosphorylated nuclear factor kappa‑light‑chain‑enhancer of activated B cells. Through the
increased hepatic activation of the nuclear factor erythroid 2‑related factor 2, meldonium improves
the antioxidative defence in the liver of animals subjected to I/R, as proved by an increase in serum
and liver ascorbic/dehydroascorbic acid ratio, hepatic haem oxygenase 1 expression, glutathione
and free thiol groups content, and hepatic copper‑zinc superoxide dismutase, manganese superoxide
dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Based on our results,
it can be concluded that meldonium represent a protective agent against I/R‑induced liver injury, with
a clinical significance in surgical procedures.
PB  - Nature Publishing Group
T2  - Scientific Reports
T1  - The effects of meldonium on the acute ischemia/reperfusion liver injury in rats
IS  - 1
VL  - 11
DO  - 10.1038/s41598-020-80011-y
SP  - 1305
ER  - 

@article{
author = "Đurašević, Siniša and Stojković, Maja and Sopta, Jelena and Pavlović, Slađan and Borković Mitić, Slavica and Ivanović, Anđelija and Jasnić, Nebojša and Tosti, Tomislav and Đurović, Saša and Đorđević, Jelena and Todorović, Zoran",
year = "2021",
abstract = "Acute ischemia/reperfusion (I/R) liver injury is a clinical condition challenging to treat. Meldonium
is an anti‑ischemic agent that shifts energy production from fatty acid oxidation to less oxygen‑
consuming glycolysis. Thus, we investigated the effects of a 4‑week meldonium pre‑treatment
(300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats. Our results showed that
meldonium ameliorates I/R‑induced liver inflammation and injury, as confirmed by liver histology,
and by attenuation of serum alanine‑ and aspartate aminotransferase activity, serum and liver
high mobility group box 1 protein expression, and liver expression of Bax/Bcl2, haptoglobin, and
the phosphorylated nuclear factor kappa‑light‑chain‑enhancer of activated B cells. Through the
increased hepatic activation of the nuclear factor erythroid 2‑related factor 2, meldonium improves
the antioxidative defence in the liver of animals subjected to I/R, as proved by an increase in serum
and liver ascorbic/dehydroascorbic acid ratio, hepatic haem oxygenase 1 expression, glutathione
and free thiol groups content, and hepatic copper‑zinc superoxide dismutase, manganese superoxide
dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Based on our results,
it can be concluded that meldonium represent a protective agent against I/R‑induced liver injury, with
a clinical significance in surgical procedures.",
publisher = "Nature Publishing Group",
journal = "Scientific Reports",
title = "The effects of meldonium on the acute ischemia/reperfusion liver injury in rats",
number = "1",
volume = "11",
doi = "10.1038/s41598-020-80011-y",
pages = "1305"
}

Đurašević, S., Stojković, M., Sopta, J., Pavlović, S., Borković Mitić, S., Ivanović, A., Jasnić, N., Tosti, T., Đurović, S., Đorđević, J.,& Todorović, Z.. (2021). The effects of meldonium on the acute ischemia/reperfusion liver injury in rats. in Scientific Reports
Nature Publishing Group., 11(1), 1305.
https://doi.org/10.1038/s41598-020-80011-y

Đurašević S, Stojković M, Sopta J, Pavlović S, Borković Mitić S, Ivanović A, Jasnić N, Tosti T, Đurović S, Đorđević J, Todorović Z. The effects of meldonium on the acute ischemia/reperfusion liver injury in rats. in Scientific Reports. 2021;11(1):1305.
doi:10.1038/s41598-020-80011-y .

Đurašević, Siniša, Stojković, Maja, Sopta, Jelena, Pavlović, Slađan, Borković Mitić, Slavica, Ivanović, Anđelija, Jasnić, Nebojša, Tosti, Tomislav, Đurović, Saša, Đorđević, Jelena, Todorović, Zoran, "The effects of meldonium on the acute ischemia/reperfusion liver injury in rats" in Scientific Reports, 11, no. 1 (2021):1305,
https://doi.org/10.1038/s41598-020-80011-y . .

TY  - JOUR
AU  - Todorović, Zoran
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Grigorov, Ilijana
AU  - Pavlović, Slađan
AU  - Jasnić, Nebojša
AU  - Tosti, Tomislav
AU  - Bjekić Macut, Jelica
AU  - Thiemermann, Christoph
AU  - Đorđević, Jelena
PY  - 2021
UR  - https://www.mdpi.com/1422-0067/22/6/2798
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4162
AB  - Lipids play an essential role in both tissue protection and damage. Tissue ischemia creates anaerobic conditions in which enzyme inactivation occurs, and reperfusion can initiate oxidative stress that leads to harmful changes in membrane lipids, the formation of aldehydes, and chain damage until cell death. The critical event in such a series of harmful events in the cell is the unwanted accumulation of fatty acids that leads to lipotoxicity. Lipid analysis provides additional insight into the pathogenesis of ischemia/reperfusion (I/R) disorders and reveals new targets for drug action. The profile of changes in the composition of fatty acids in the cell, as well as the time course of these changes, indicate both the mechanism of damage and new therapeutic possibilities. A therapeutic approach to reperfusion lipotoxicity involves attenuation of fatty acids overload, i.e., their transport to adipose tissue and/or inhibition of the adverse effects of fatty acids on cell damage and death. The latter option involves using PPAR agonists and drugs that modulate the transport of fatty acids via carnitine into the interior of the mitochondria or the redirection of long-chain fatty acids to peroxisomes.
PB  - Multidisciplinary Digital Publishing Institute
T2  - International Journal of Molecular Sciences
T1  - Lipidomics Provides New Insight into Pathogenesis and Therapeutic Targets of the Ischemia—Reperfusion Injury
IS  - 6
VL  - 22
DO  - 10.3390/ijms22062798
SP  - 2798
ER  - 

@article{
author = "Todorović, Zoran and Đurašević, Siniša and Stojković, Maja and Grigorov, Ilijana and Pavlović, Slađan and Jasnić, Nebojša and Tosti, Tomislav and Bjekić Macut, Jelica and Thiemermann, Christoph and Đorđević, Jelena",
year = "2021",
abstract = "Lipids play an essential role in both tissue protection and damage. Tissue ischemia creates anaerobic conditions in which enzyme inactivation occurs, and reperfusion can initiate oxidative stress that leads to harmful changes in membrane lipids, the formation of aldehydes, and chain damage until cell death. The critical event in such a series of harmful events in the cell is the unwanted accumulation of fatty acids that leads to lipotoxicity. Lipid analysis provides additional insight into the pathogenesis of ischemia/reperfusion (I/R) disorders and reveals new targets for drug action. The profile of changes in the composition of fatty acids in the cell, as well as the time course of these changes, indicate both the mechanism of damage and new therapeutic possibilities. A therapeutic approach to reperfusion lipotoxicity involves attenuation of fatty acids overload, i.e., their transport to adipose tissue and/or inhibition of the adverse effects of fatty acids on cell damage and death. The latter option involves using PPAR agonists and drugs that modulate the transport of fatty acids via carnitine into the interior of the mitochondria or the redirection of long-chain fatty acids to peroxisomes.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "International Journal of Molecular Sciences",
title = "Lipidomics Provides New Insight into Pathogenesis and Therapeutic Targets of the Ischemia—Reperfusion Injury",
number = "6",
volume = "22",
doi = "10.3390/ijms22062798",
pages = "2798"
}

Todorović, Z., Đurašević, S., Stojković, M., Grigorov, I., Pavlović, S., Jasnić, N., Tosti, T., Bjekić Macut, J., Thiemermann, C.,& Đorđević, J.. (2021). Lipidomics Provides New Insight into Pathogenesis and Therapeutic Targets of the Ischemia—Reperfusion Injury. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute., 22(6), 2798.
https://doi.org/10.3390/ijms22062798

Todorović Z, Đurašević S, Stojković M, Grigorov I, Pavlović S, Jasnić N, Tosti T, Bjekić Macut J, Thiemermann C, Đorđević J. Lipidomics Provides New Insight into Pathogenesis and Therapeutic Targets of the Ischemia—Reperfusion Injury. in International Journal of Molecular Sciences. 2021;22(6):2798.
doi:10.3390/ijms22062798 .

Todorović, Zoran, Đurašević, Siniša, Stojković, Maja, Grigorov, Ilijana, Pavlović, Slađan, Jasnić, Nebojša, Tosti, Tomislav, Bjekić Macut, Jelica, Thiemermann, Christoph, Đorđević, Jelena, "Lipidomics Provides New Insight into Pathogenesis and Therapeutic Targets of the Ischemia—Reperfusion Injury" in International Journal of Molecular Sciences, 22, no. 6 (2021):2798,
https://doi.org/10.3390/ijms22062798 . .

TY  - JOUR
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Mitić, Bojan
AU  - Manojlović, Dragan
AU  - Pavlović, Slađan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4344
AB  - There are only a few reports examining the impact of oxidative stress in patients with benign and
malignant brain tumors. In this study we investigated whether there are changes in antioxidant
system (AOS) parameters and key trace elements between control, benign and malignant brain
tissues. The study also aimed to examine correlations between the analyzed parameters. The study
enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results
were compared with control tissue (CT) without tumor infiltration collected from patients with BT.
The following antioxidant parameters were determined: activities of total, manganese‑containing,
and copper/zinc‑containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of
catalase, glutathione peroxidase, glutathione S‑transferase, glutathione reductase and acetylcholine
esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn),
copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS
parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes
in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high
GST activities were significant for distinguishing between MT and CT. Malignant tissue was also
characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis
clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT
and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the
MT group. The results of this study reveal differences between the AOS parameters and the essential
trace elements between the analyzed groups. The observed dysregulations show that oxidative stress
could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of
benign and malignant brain tumors.
PB  - Springer Nature
T2  - Scientifc Reports
T1  - Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues
VL  - 11
DO  - 10.1038/s41598-021-94302-5
SP  - 14776
ER  - 

@article{
author = "Borković Mitić, Slavica and Stojsavljević, Aleksandar and Vujotić, Ljiljana and Matić, Siniša and Mitić, Bojan and Manojlović, Dragan and Pavlović, Slađan",
year = "2021",
abstract = "There are only a few reports examining the impact of oxidative stress in patients with benign and
malignant brain tumors. In this study we investigated whether there are changes in antioxidant
system (AOS) parameters and key trace elements between control, benign and malignant brain
tissues. The study also aimed to examine correlations between the analyzed parameters. The study
enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results
were compared with control tissue (CT) without tumor infiltration collected from patients with BT.
The following antioxidant parameters were determined: activities of total, manganese‑containing,
and copper/zinc‑containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of
catalase, glutathione peroxidase, glutathione S‑transferase, glutathione reductase and acetylcholine
esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn),
copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS
parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes
in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high
GST activities were significant for distinguishing between MT and CT. Malignant tissue was also
characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis
clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT
and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the
MT group. The results of this study reveal differences between the AOS parameters and the essential
trace elements between the analyzed groups. The observed dysregulations show that oxidative stress
could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of
benign and malignant brain tumors.",
publisher = "Springer Nature",
journal = "Scientifc Reports",
title = "Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues",
volume = "11",
doi = "10.1038/s41598-021-94302-5",
pages = "14776"
}

Borković Mitić, S., Stojsavljević, A., Vujotić, L., Matić, S., Mitić, B., Manojlović, D.,& Pavlović, S.. (2021). Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues. in Scientifc Reports
Springer Nature., 11, 14776.
https://doi.org/10.1038/s41598-021-94302-5

Borković Mitić S, Stojsavljević A, Vujotić L, Matić S, Mitić B, Manojlović D, Pavlović S. Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues. in Scientifc Reports. 2021;11:14776.
doi:10.1038/s41598-021-94302-5 .

Borković Mitić, Slavica, Stojsavljević, Aleksandar, Vujotić, Ljiljana, Matić, Siniša, Mitić, Bojan, Manojlović, Dragan, Pavlović, Slađan, "Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues" in Scientifc Reports, 11 (2021):14776,
https://doi.org/10.1038/s41598-021-94302-5 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Lakić, Iva
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4413
AB  - Sepsis is a life-threatening condition caused by the dysregulated and overwhelming
response to infection, accompanied by an exaggerated pro-inflammatory state and lipid metabolism
disturbance leading to sequential organ failure. Meldonium is an anti-ischemic and anti-inflammatory
agent which negatively interferes with lipid metabolism by shifting energy production from fatty
acid oxidation to glycolysis, as a less oxygen-demanding pathway. Thus, we investigated the effects
of a four-week meldonium pre-treatment on faecal-induced sepsis in Sprague-Dawley male rats.
Surprisingly, under septic conditions, meldonium increased animal mortality rate compared with
the meldonium non-treated group. However, analysis of the tissue oxidative status did not provide
support for the detrimental effects of meldonium, nor did the analysis of the tissue inflammatory
status showing anti-inflammatory, anti-apoptotic, and anti-necrotic effects of meldonium. After
performing tissue lipidomic analysis, we concluded that the potential cause of the meldonium
harmful effect is to be found in the overall decreased lipid metabolism. The present study underlines
the importance of uninterrupted energy production in sepsis, closely drawing attention to the possible
harmful effects of lipid-mobilization impairment caused by certain therapeutics. This could lead to
the much-needed revision of the existing guidelines in the clinical treatment of sepsis while paving
the way for discovering new therapeutic approaches.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats
IS  - 18
VL  - 22
DO  - 10.3390/ijms22189698
SP  - 9698
ER  - 

@article{
author = "Đurašević, Siniša and Ružičić, Aleksandra and Lakić, Iva and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Stanković, Sanja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2021",
abstract = "Sepsis is a life-threatening condition caused by the dysregulated and overwhelming
response to infection, accompanied by an exaggerated pro-inflammatory state and lipid metabolism
disturbance leading to sequential organ failure. Meldonium is an anti-ischemic and anti-inflammatory
agent which negatively interferes with lipid metabolism by shifting energy production from fatty
acid oxidation to glycolysis, as a less oxygen-demanding pathway. Thus, we investigated the effects
of a four-week meldonium pre-treatment on faecal-induced sepsis in Sprague-Dawley male rats.
Surprisingly, under septic conditions, meldonium increased animal mortality rate compared with
the meldonium non-treated group. However, analysis of the tissue oxidative status did not provide
support for the detrimental effects of meldonium, nor did the analysis of the tissue inflammatory
status showing anti-inflammatory, anti-apoptotic, and anti-necrotic effects of meldonium. After
performing tissue lipidomic analysis, we concluded that the potential cause of the meldonium
harmful effect is to be found in the overall decreased lipid metabolism. The present study underlines
the importance of uninterrupted energy production in sepsis, closely drawing attention to the possible
harmful effects of lipid-mobilization impairment caused by certain therapeutics. This could lead to
the much-needed revision of the existing guidelines in the clinical treatment of sepsis while paving
the way for discovering new therapeutic approaches.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats",
number = "18",
volume = "22",
doi = "10.3390/ijms22189698",
pages = "9698"
}

Đurašević, S., Ružičić, A., Lakić, I., Tosti, T., Đurović, S., Glumac, S., Pavlović, S., Borković Mitić, S., Grigorov, I., Stanković, S., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2021). The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats. in International Journal of Molecular Sciences
Basel: MDPI., 22(18), 9698.
https://doi.org/10.3390/ijms22189698

Đurašević S, Ružičić A, Lakić I, Tosti T, Đurović S, Glumac S, Pavlović S, Borković Mitić S, Grigorov I, Stanković S, Jasnić N, Đorđević J, Todorović Z. The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats. in International Journal of Molecular Sciences. 2021;22(18):9698.
doi:10.3390/ijms22189698 .

Đurašević, Siniša, Ružičić, Aleksandra, Lakić, Iva, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Stanković, Sanja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats" in International Journal of Molecular Sciences, 22, no. 18 (2021):9698,
https://doi.org/10.3390/ijms22189698 . .

TY  - CONF
AU  - Lakić, Iva
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Todorović, Zoran
AU  - Đorđević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4896
AB  - Background: Sepsis is a life-threatening condition caused by the dysregulated and overwhelming response to infection, accompanied by exaggerated pro-inflammatory state and lipid metabolism disturbance leading to sequential organ failure.1, 2 Meldonium is an anti-ischemic and anti-inflammatory agent, clinically used to treat myocardial ischemia.3 By shifting energy production from fatty acid oxidation to glycolysis, as an oxygen less consuming pathway, meldonium interferes negatively with lipid metabolism. 
Methods: Thus, we investigated the effects of a 4-week meldonium pre-treatment in 300 mg/kg b.m./day dosage on the course of the sepsis induced by a single intraperitoneal injection of faeces (0.5 g faeces/1 mL saline/100 g b.m.)in Sprague-Dawley male rats. The degree of the heart injury was evaluated by measuring tissue pro-apoptotic Bax and anti-apoptotic Bcl-2 ratio, tissue level of the necrotic marker - high mobility group box 1 protein level (HMGB1), together with the heart histology analysis. Sepsis-associated heart inflammation
was assessed by measuring level of an activated form of NF-kB p65 (phospho-NF-κB p65).
Results: In the heart whole homogenates of the septic group of animals (S) HMGB1 level increased 1.7-fold, in comparison to control rats, while meldonium reduced sepsis-induced increase by 18 % (M+S). The underlying mechanism of the proinflammatory action of HMGB1 includes activation of NF-κB signalling
pathways tissue, so the increased HMGB1 level was followed by a 1.4-fold increase of p-NF-κB p65 in the heart of the S group of rats and a 19 % decreased in the heart of M+S group. The apoptotic marker Bax/Bcl-2 ratio changed in the same manner: 1.4-fold increase in the heart of animals from the S group and a 32 % decrease in the heart of the M+S group. On the other hand, heart histology analysis shows that meldonium worsened the heart histological score, causing the severe and diffuse interstitial mononuclear infiltration along with a greater loss of myocytes and myofibrillar contraction band necrosis. The heart lipidomic analysis suggests that meldonium exhibits potentially harmful effects under septic condition due to the lipid-mobilization impairment.
Conclusion: Meldonium exerted anti-inflammatory, anti-apoptotic, and anti- necrotic effects, while it worsened the septic rat heart histology.
PB  - Banja Luka: Association of Medical Doctors
PB  - Banja Luka: Faculty of Medicine, University of Banja Luka
C3  - 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina
T1  - The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4896
ER  - 

@conference{
author = "Lakić, Iva and Đurašević, Siniša and Ružičić, Aleksandra and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Stanković, Sanja and Jasnić, Nebojša and Todorović, Zoran and Đorđević, Jelena",
year = "2021",
abstract = "Background: Sepsis is a life-threatening condition caused by the dysregulated and overwhelming response to infection, accompanied by exaggerated pro-inflammatory state and lipid metabolism disturbance leading to sequential organ failure.1, 2 Meldonium is an anti-ischemic and anti-inflammatory agent, clinically used to treat myocardial ischemia.3 By shifting energy production from fatty acid oxidation to glycolysis, as an oxygen less consuming pathway, meldonium interferes negatively with lipid metabolism. 
Methods: Thus, we investigated the effects of a 4-week meldonium pre-treatment in 300 mg/kg b.m./day dosage on the course of the sepsis induced by a single intraperitoneal injection of faeces (0.5 g faeces/1 mL saline/100 g b.m.)in Sprague-Dawley male rats. The degree of the heart injury was evaluated by measuring tissue pro-apoptotic Bax and anti-apoptotic Bcl-2 ratio, tissue level of the necrotic marker - high mobility group box 1 protein level (HMGB1), together with the heart histology analysis. Sepsis-associated heart inflammation
was assessed by measuring level of an activated form of NF-kB p65 (phospho-NF-κB p65).
Results: In the heart whole homogenates of the septic group of animals (S) HMGB1 level increased 1.7-fold, in comparison to control rats, while meldonium reduced sepsis-induced increase by 18 % (M+S). The underlying mechanism of the proinflammatory action of HMGB1 includes activation of NF-κB signalling
pathways tissue, so the increased HMGB1 level was followed by a 1.4-fold increase of p-NF-κB p65 in the heart of the S group of rats and a 19 % decreased in the heart of M+S group. The apoptotic marker Bax/Bcl-2 ratio changed in the same manner: 1.4-fold increase in the heart of animals from the S group and a 32 % decrease in the heart of the M+S group. On the other hand, heart histology analysis shows that meldonium worsened the heart histological score, causing the severe and diffuse interstitial mononuclear infiltration along with a greater loss of myocytes and myofibrillar contraction band necrosis. The heart lipidomic analysis suggests that meldonium exhibits potentially harmful effects under septic condition due to the lipid-mobilization impairment.
Conclusion: Meldonium exerted anti-inflammatory, anti-apoptotic, and anti- necrotic effects, while it worsened the septic rat heart histology.",
publisher = "Banja Luka: Association of Medical Doctors, Banja Luka: Faculty of Medicine, University of Banja Luka",
journal = "7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina",
title = "The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4896"
}

Lakić, I., Đurašević, S., Ružičić, A., Tosti, T., Đurović, S., Glumac, S., Pavlović, S., Borković Mitić, S., Grigorov, I., Stanković, S., Jasnić, N., Todorović, Z.,& Đorđević, J.. (2021). The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury. in 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina
Banja Luka: Association of Medical Doctors., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_4896

Lakić I, Đurašević S, Ružičić A, Tosti T, Đurović S, Glumac S, Pavlović S, Borković Mitić S, Grigorov I, Stanković S, Jasnić N, Todorović Z, Đorđević J. The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury. in 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina. 2021;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_4896 .

Lakić, Iva, Đurašević, Siniša, Ružičić, Aleksandra, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Stanković, Sanja, Jasnić, Nebojša, Todorović, Zoran, Đorđević, Jelena, "The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury" in 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina (2021):57,
https://hdl.handle.net/21.15107/rcub_ibiss_4896 .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Krizmanić, Imre
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mitić, Bojan
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4054
AB  - We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione
S-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from two
localities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We also
determined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations of
manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium
(U). The obtained results show that animals from VSB had significantly lower weights and lengths than
animals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while the
SH content was significantly lower in animals from VSB compared to those from AVS and ABP. No
correlations between trace-element concentrations in water and animal tissue were observed. We concluded
that the obtained results were more likely a consequence of the combination of developmental differences
and the effects of different habitat conditions, environmental and anthropogenic influences than of
concentrations of trace elements in the water alone.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)
IS  - 4
VL  - 72
DO  - 10.2298/ABS200825043P
SP  - 491
EP  - 501
ER  - 

@article{
author = "Pavlović, Slađan and Krizmanić, Imre and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mitić, Bojan",
year = "2020",
abstract = "We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione
S-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from two
localities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We also
determined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations of
manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium
(U). The obtained results show that animals from VSB had significantly lower weights and lengths than
animals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while the
SH content was significantly lower in animals from VSB compared to those from AVS and ABP. No
correlations between trace-element concentrations in water and animal tissue were observed. We concluded
that the obtained results were more likely a consequence of the combination of developmental differences
and the effects of different habitat conditions, environmental and anthropogenic influences than of
concentrations of trace elements in the water alone.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)",
number = "4",
volume = "72",
doi = "10.2298/ABS200825043P",
pages = "491-501"
}

Pavlović, S., Krizmanić, I., Borković Mitić, S., Stojsavljević, A.,& Mitić, B.. (2020). A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 72(4), 491-501.
https://doi.org/10.2298/ABS200825043P

Pavlović S, Krizmanić I, Borković Mitić S, Stojsavljević A, Mitić B. A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences. 2020;72(4):491-501.
doi:10.2298/ABS200825043P .

Pavlović, Slađan, Krizmanić, Imre, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mitić, Bojan, "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)" in Archives of Biological Sciences, 72, no. 4 (2020):491-501,
https://doi.org/10.2298/ABS200825043P . .

TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2020
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3713
AB  - IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
IS  - 4
VL  - 16
DO  - 10.5114/aoms.2019.87409
SP  - 811
EP  - 819
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2020",
abstract = "IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
number = "4",
volume = "16",
doi = "10.5114/aoms.2019.87409",
pages = "811-819"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2020). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach., 16(4), 811-819.
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2020;16(4):811-819.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science, 16, no. 4 (2020):811-819,
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Bogdanović, Ljiljana
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Jasnić, Nebojša
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3519
AB  - Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to
treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation
to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week
meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our
results showed that meldonium decreased animal body mass gain, food and water intake, and
carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium
increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and
increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1,
causing manganese superoxide dismutase expression and activity to increase, as well as lipid
peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase
activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum
high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in
I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and
serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex
changes in renal lipidomics. Taken together, our results have confirmed that meldonium pretreatment protects against I/R-induced oxidative stress and apoptosis/necrosis.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of meldonium on the renal acute ischemia/reperfusion injury in rats
IS  - 22
VL  - 20
DO  - 10.3390/ijms20225747
SP  - 5747
ER  - 

@article{
author = "Đurašević, Siniša and Stojković, Maja and Bogdanović, Ljiljana and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Bogojević, Desanka and Jasnić, Nebojša and Tosti, Tomislav and Đurović, Saša and Đorđević, Jelena and Todorović, Zoran",
year = "2019",
abstract = "Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to
treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation
to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week
meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our
results showed that meldonium decreased animal body mass gain, food and water intake, and
carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium
increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and
increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1,
causing manganese superoxide dismutase expression and activity to increase, as well as lipid
peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase
activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum
high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in
I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and
serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex
changes in renal lipidomics. Taken together, our results have confirmed that meldonium pretreatment protects against I/R-induced oxidative stress and apoptosis/necrosis.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of meldonium on the renal acute ischemia/reperfusion injury in rats",
number = "22",
volume = "20",
doi = "10.3390/ijms20225747",
pages = "5747"
}

Đurašević, S., Stojković, M., Bogdanović, L., Pavlović, S., Borković Mitić, S., Grigorov, I., Bogojević, D., Jasnić, N., Tosti, T., Đurović, S., Đorđević, J.,& Todorović, Z.. (2019). The effects of meldonium on the renal acute ischemia/reperfusion injury in rats. in International Journal of Molecular Sciences
MDPI., 20(22), 5747.
https://doi.org/10.3390/ijms20225747

Đurašević S, Stojković M, Bogdanović L, Pavlović S, Borković Mitić S, Grigorov I, Bogojević D, Jasnić N, Tosti T, Đurović S, Đorđević J, Todorović Z. The effects of meldonium on the renal acute ischemia/reperfusion injury in rats. in International Journal of Molecular Sciences. 2019;20(22):5747.
doi:10.3390/ijms20225747 .

Đurašević, Siniša, Stojković, Maja, Bogdanović, Ljiljana, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Bogojević, Desanka, Jasnić, Nebojša, Tosti, Tomislav, Đurović, Saša, Đorđević, Jelena, Todorović, Zoran, "The effects of meldonium on the renal acute ischemia/reperfusion injury in rats" in International Journal of Molecular Sciences, 20, no. 22 (2019):5747,
https://doi.org/10.3390/ijms20225747 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3365
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
VL  - 223
DO  - 10.1016/J.CBPC.2019.05.010
SP  - 26
EP  - 34
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
volume = "223",
doi = "10.1016/J.CBPC.2019.05.010",
pages = "26-34"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223, 26-34.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;223:26-34.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223 (2019):26-34,
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - CHAP
AU  - Đurašević, Siniša
AU  - Todorović, Zoran
AU  - Pavlović, Slađan
AU  - Pejić, Snežana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/B9780128144664000276?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3415
AB  - Oxidative stress (OS) represents an imbalance of free radicals production and antioxidant defense in which the extensive pro-oxidant production may cause damage to cell structures. OS is the major event occurring in pathogenesis of liver disorders, ranging from metabolic to proliferative disorders. Cadmium is a highly toxic and widespread toxicant. Cadmium itself is unable to generate free radicals directly, as cadmium does not undergo redox cycling. However, indirect generation of various radicals involving the superoxide radical, hydroxyl radical, and nitric oxide has been reported. One of the biologically most relevant features of C60 fullerene is the ability to quench various free radicals, behaving as a “free radical sponge.” Conversely, photosensitization of C60 yields highly reactive singlet oxygen or superoxide anion. Therefore, the dual property of fullerene to either quench or generate cell-damaging reactive oxygen species could be potentially exploited for their development as cytoprotective or cytotoxic agents.
PB  - Academic Press
T2  - Dietary Interventions in Liver Disease
T1  - Cadmium and Fullerenes in Liver Diseases
DO  - 10.1016/B978-0-12-814466-4.00027-6
SP  - 333
EP  - 344
ER  - 

@inbook{
editor = "Watson, Ronald Ross, Preedy, Victor R.",
author = "Đurašević, Siniša and Todorović, Zoran and Pavlović, Slađan and Pejić, Snežana",
year = "2019",
abstract = "Oxidative stress (OS) represents an imbalance of free radicals production and antioxidant defense in which the extensive pro-oxidant production may cause damage to cell structures. OS is the major event occurring in pathogenesis of liver disorders, ranging from metabolic to proliferative disorders. Cadmium is a highly toxic and widespread toxicant. Cadmium itself is unable to generate free radicals directly, as cadmium does not undergo redox cycling. However, indirect generation of various radicals involving the superoxide radical, hydroxyl radical, and nitric oxide has been reported. One of the biologically most relevant features of C60 fullerene is the ability to quench various free radicals, behaving as a “free radical sponge.” Conversely, photosensitization of C60 yields highly reactive singlet oxygen or superoxide anion. Therefore, the dual property of fullerene to either quench or generate cell-damaging reactive oxygen species could be potentially exploited for their development as cytoprotective or cytotoxic agents.",
publisher = "Academic Press",
journal = "Dietary Interventions in Liver Disease",
booktitle = "Cadmium and Fullerenes in Liver Diseases",
doi = "10.1016/B978-0-12-814466-4.00027-6",
pages = "333-344"
}

Watson, R. R., Preedy, V. R., Đurašević, S., Todorović, Z., Pavlović, S.,& Pejić, S.. (2019). Cadmium and Fullerenes in Liver Diseases. in Dietary Interventions in Liver Disease
Academic Press., 333-344.
https://doi.org/10.1016/B978-0-12-814466-4.00027-6

Watson RR, Preedy VR, Đurašević S, Todorović Z, Pavlović S, Pejić S. Cadmium and Fullerenes in Liver Diseases. in Dietary Interventions in Liver Disease. 2019;:333-344.
doi:10.1016/B978-0-12-814466-4.00027-6 .

Watson, Ronald Ross, Preedy, Victor R., Đurašević, Siniša, Todorović, Zoran, Pavlović, Slađan, Pejić, Snežana, "Cadmium and Fullerenes in Liver Diseases" in Dietary Interventions in Liver Disease (2019):333-344,
https://doi.org/10.1016/B978-0-12-814466-4.00027-6 . .