TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6555
AB  - Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.
PB  - Elsevier Ltd.
T2  - Medical Hypotheses
T1  - Can food affect the perception of time?
VL  - 184
DO  - 10.1016/j.mehy.2024.111283
SP  - 111283
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2024",
abstract = "Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.",
publisher = "Elsevier Ltd.",
journal = "Medical Hypotheses",
title = "Can food affect the perception of time?",
volume = "184",
doi = "10.1016/j.mehy.2024.111283",
pages = "111283"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2024). Can food affect the perception of time?. in Medical Hypotheses
Elsevier Ltd.., 184, 111283.
https://doi.org/10.1016/j.mehy.2024.111283

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. Can food affect the perception of time?. in Medical Hypotheses. 2024;184:111283.
doi:10.1016/j.mehy.2024.111283 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "Can food affect the perception of time?" in Medical Hypotheses, 184 (2024):111283,
https://doi.org/10.1016/j.mehy.2024.111283 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6554
AB  - Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.
PB  - Elsevier Ltd.
T2  - Medical Hypotheses
T1  - Can food affect the perception of time?
VL  - 184
DO  - 10.1016/j.mehy.2024.111283
SP  - 111283
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2024",
abstract = "Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.",
publisher = "Elsevier Ltd.",
journal = "Medical Hypotheses",
title = "Can food affect the perception of time?",
volume = "184",
doi = "10.1016/j.mehy.2024.111283",
pages = "111283"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2024). Can food affect the perception of time?. in Medical Hypotheses
Elsevier Ltd.., 184, 111283.
https://doi.org/10.1016/j.mehy.2024.111283

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. Can food affect the perception of time?. in Medical Hypotheses. 2024;184:111283.
doi:10.1016/j.mehy.2024.111283 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "Can food affect the perception of time?" in Medical Hypotheses, 184 (2024):111283,
https://doi.org/10.1016/j.mehy.2024.111283 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Milenkovic, Dragan
AU  - Cesar, Thais
AU  - Filipović R., Miloš
AU  - Milošević, Verica
PY  - 2024
UR  - https://iubmb.onlinelibrary.wiley.com/journal/18728081
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6462
AB  - Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.
PB  - John Wiley and Sons
T2  - Biofactors
T1  - Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats
DO  - 10.1002/biof.2038
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Milenkovic, Dragan and Cesar, Thais and Filipović R., Miloš and Milošević, Verica",
year = "2024",
abstract = "Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.",
publisher = "John Wiley and Sons",
journal = "Biofactors",
title = "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats",
doi = "10.1002/biof.2038"
}

Miler, M., Živanović, J., Ajdžanović, V., Milenkovic, D., Cesar, T., Filipović R., M.,& Milošević, V.. (2024). Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors
John Wiley and Sons..
https://doi.org/10.1002/biof.2038

Miler M, Živanović J, Ajdžanović V, Milenkovic D, Cesar T, Filipović R. M, Milošević V. Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors. 2024;.
doi:10.1002/biof.2038 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Milenkovic, Dragan, Cesar, Thais, Filipović R., Miloš, Milošević, Verica, "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats" in Biofactors (2024),
https://doi.org/10.1002/biof.2038 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Trifunović, Svetlana
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Jovanović, Ljubiša
AU  - Ristić, Nataša
AU  - Ljiljana, Marina
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6120
AB  - Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age
IS  - 16
VL  - 24
DO  - 10.3390/ijms241612624
SP  - 12624
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Trifunović, Svetlana and Pavlović, Slađan and Borković-Mitić, Slavica and Jovanović, Ljubiša and Ristić, Nataša and Ljiljana, Marina and Ajdžanović, Vladimir and Filipović, Branko",
year = "2023",
abstract = "Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age",
number = "16",
volume = "24",
doi = "10.3390/ijms241612624",
pages = "12624"
}

Šošić-Jurjević, B., Lütjohann, D., Trifunović, S., Pavlović, S., Borković-Mitić, S., Jovanović, L., Ristić, N., Ljiljana, M., Ajdžanović, V.,& Filipović, B.. (2023). Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences
Basel: MDPI., 24(16), 12624.
https://doi.org/10.3390/ijms241612624

Šošić-Jurjević B, Lütjohann D, Trifunović S, Pavlović S, Borković-Mitić S, Jovanović L, Ristić N, Ljiljana M, Ajdžanović V, Filipović B. Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences. 2023;24(16):12624.
doi:10.3390/ijms241612624 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Trifunović, Svetlana, Pavlović, Slađan, Borković-Mitić, Slavica, Jovanović, Ljubiša, Ristić, Nataša, Ljiljana, Marina, Ajdžanović, Vladimir, Filipović, Branko, "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age" in International Journal of Molecular Sciences, 24, no. 16 (2023):12624,
https://doi.org/10.3390/ijms241612624 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Čukuranović-Kokoris, Jovana
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6405
AB  - Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca.
AB  - Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze
T1  - Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause
SP  - 166
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6405
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Čukuranović-Kokoris, Jovana and Miler, Marko and Šošić-Jurjević, Branka and Živanović, Jasmina and Marković Filipović, Jelena and Milošević, Verica and Filipović, Branko",
year = "2023",
abstract = "Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca., Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze, Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause",
pages = "166-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6405"
}

Ajdžanović, V., Čukuranović-Kokoris, J., Miler, M., Šošić-Jurjević, B., Živanović, J., Marković Filipović, J., Milošević, V.,& Filipović, B.. (2023). Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405

Ajdžanović V, Čukuranović-Kokoris J, Miler M, Šošić-Jurjević B, Živanović J, Marković Filipović J, Milošević V, Filipović B. Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

Ajdžanović, Vladimir, Čukuranović-Kokoris, Jovana, Miler, Marko, Šošić-Jurjević, Branka, Živanović, Jasmina, Marković Filipović, Jelena, Milošević, Verica, Filipović, Branko, "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):166-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6279
AB  - Prostate health is related to the frequency of ejaculation and prostate drainage. Frequent, repetitive ejaculations theoretically may pose a prostate cancer risk due to ensuing high testosterone levels and loss of zinc. In line with this, the proximity of the human rectum and prostate is of diagnostic and therapeutic importance. We provide evidence to support the view that the proximity of the rectum and prostate in men enables lower bowel distention to compress the prostate's lateral side to facilitate drainage, which might lower cancer risk. This drainage mechanism is likely on-going and gradual, compared to rapid drainage following ejaculation. The physiological context suggests that these mechanisms of prostate drainage are complementary. Dietary content containing polyphenol- and fiber-rich meals, along with voluminous stool formation and increased gastrointestinal transit may all help improve prostate drainage. This may be important in voluntary abstinent men or other categories of sexually limited men. Recognizing that the anatomic relationship between the rectal area and prostate may facilitate prostate drainage, because of diet and gastro-intestinal activity, could be important to prostate health maintenance.
PB  - MRE Press
T2  - Journal of Men's Health
T1  - The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health
IS  - 10
VL  - 19
DO  - 10.22514/jomh.2023.094
SP  - 1
EP  - 6
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2023",
abstract = "Prostate health is related to the frequency of ejaculation and prostate drainage. Frequent, repetitive ejaculations theoretically may pose a prostate cancer risk due to ensuing high testosterone levels and loss of zinc. In line with this, the proximity of the human rectum and prostate is of diagnostic and therapeutic importance. We provide evidence to support the view that the proximity of the rectum and prostate in men enables lower bowel distention to compress the prostate's lateral side to facilitate drainage, which might lower cancer risk. This drainage mechanism is likely on-going and gradual, compared to rapid drainage following ejaculation. The physiological context suggests that these mechanisms of prostate drainage are complementary. Dietary content containing polyphenol- and fiber-rich meals, along with voluminous stool formation and increased gastrointestinal transit may all help improve prostate drainage. This may be important in voluntary abstinent men or other categories of sexually limited men. Recognizing that the anatomic relationship between the rectal area and prostate may facilitate prostate drainage, because of diet and gastro-intestinal activity, could be important to prostate health maintenance.",
publisher = "MRE Press",
journal = "Journal of Men's Health",
title = "The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health",
number = "10",
volume = "19",
doi = "10.22514/jomh.2023.094",
pages = "1-6"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2023). The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health. in Journal of Men's Health
MRE Press., 19(10), 1-6.
https://doi.org/10.22514/jomh.2023.094

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health. in Journal of Men's Health. 2023;19(10):1-6.
doi:10.22514/jomh.2023.094 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health" in Journal of Men's Health, 19, no. 10 (2023):1-6,
https://doi.org/10.22514/jomh.2023.094 . .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Dovenska, Monika
AU  - Ajdžanović, Vladimir
AU  - Marković Filipović, Jelena
AU  - Ugrenović, Slađana
AU  - Kundalić, Braca
AU  - Milošević, Verica
AU  - Popovska-Perčinić, Florina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6278
AB  - Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6278
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Dovenska, Monika and Ajdžanović, Vladimir and Marković Filipović, Jelena and Ugrenović, Slađana and Kundalić, Braca and Milošević, Verica and Popovska-Perčinić, Florina",
year = "2023",
abstract = "Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6278"
}

Čukuranović-Kokoris, J., Dovenska, M., Ajdžanović, V., Marković Filipović, J., Ugrenović, S., Kundalić, B., Milošević, V.,& Popovska-Perčinić, F.. (2023). Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278

Čukuranović-Kokoris J, Dovenska M, Ajdžanović V, Marković Filipović J, Ugrenović S, Kundalić B, Milošević V, Popovska-Perčinić F. Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

Čukuranović-Kokoris, Jovana, Dovenska, Monika, Ajdžanović, Vladimir, Marković Filipović, Jelena, Ugrenović, Slađana, Kundalić, Braca, Milošević, Verica, Popovska-Perčinić, Florina, "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):160,
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Stanković Đorević, Dobrila
AU  - Čukuranović, Rade
AU  - Milošević, Verica
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6229
AB  - Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.
PB  - bioscientifica
C3  - 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
T1  - Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause
DO  - 10.1530/endoabs.90.EP63
SP  - 394
EP  - 395
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Miler, Marko and Živanović, Jasmina and Marković Filipović, Jelena and Stanković Đorević, Dobrila and Čukuranović, Rade and Milošević, Verica and Filipović, Branko and Ajdžanović, Vladimir",
year = "2023",
abstract = "Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.",
publisher = "bioscientifica",
journal = "25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey",
title = "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause",
doi = "10.1530/endoabs.90.EP63",
pages = "394-395"
}

Čukuranović-Kokoris, J., Đorđević, M., Miler, M., Živanović, J., Marković Filipović, J., Stanković Đorević, D., Čukuranović, R., Milošević, V., Filipović, B.,& Ajdžanović, V.. (2023). Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
bioscientifica., 394-395.
https://doi.org/10.1530/endoabs.90.EP63

Čukuranović-Kokoris J, Đorđević M, Miler M, Živanović J, Marković Filipović J, Stanković Đorević D, Čukuranović R, Milošević V, Filipović B, Ajdžanović V. Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey. 2023;:394-395.
doi:10.1530/endoabs.90.EP63 .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Miler, Marko, Živanović, Jasmina, Marković Filipović, Jelena, Stanković Đorević, Dobrila, Čukuranović, Rade, Milošević, Verica, Filipović, Branko, Ajdžanović, Vladimir, "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause" in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey (2023):394-395,
https://doi.org/10.1530/endoabs.90.EP63 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Ranin, Jovan
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6078
AB  - In his Theory of relativity, Einstein determined that the time is relative to the reference frame of the observer. Under specific conditions, there is a difference in the elapsed time between two clocks, known as time dilation. A similar relativistic effect could be attributed to the brain operating at different frequencies, e.g. while it is slow and during the thought process. Time flow and the aging process are causally linked. Herein, we introduce physical relativity into the mind/thought context and elaborate changed perception of the time flow (the impression of the time acceleration) with aging. The phenomenology of time is observed in the context of physical and biological clock, as well as by introducing the category of ‘mind time’. Mental processing impairment is crucial for the “aging-caused relativity of time”, while adjusting of its’ perception seems to be a matter of body/mind rest, mental hygiene and physical activity of the aging subject. We also provide a brief overview of the perception of time flow in some disease states that coincide with aging. Our main idea has a perspective for future development in the interdisciplinary synergy of philosophy, physical-mathematical elaboration, experimental biology and clinical investigations.
PB  - Heidelberg: Springer Nature
T2  - Biologia Futura
T1  - Biologia Futura: does the aging process contribute to the relativity of time?
IS  - 74
IS  - 1-2
DO  - 10.1007/s42977-023-00167-2
SP  - 137
EP  - 143
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Ranin, Jovan and Filipović, Branko",
year = "2023",
abstract = "In his Theory of relativity, Einstein determined that the time is relative to the reference frame of the observer. Under specific conditions, there is a difference in the elapsed time between two clocks, known as time dilation. A similar relativistic effect could be attributed to the brain operating at different frequencies, e.g. while it is slow and during the thought process. Time flow and the aging process are causally linked. Herein, we introduce physical relativity into the mind/thought context and elaborate changed perception of the time flow (the impression of the time acceleration) with aging. The phenomenology of time is observed in the context of physical and biological clock, as well as by introducing the category of ‘mind time’. Mental processing impairment is crucial for the “aging-caused relativity of time”, while adjusting of its’ perception seems to be a matter of body/mind rest, mental hygiene and physical activity of the aging subject. We also provide a brief overview of the perception of time flow in some disease states that coincide with aging. Our main idea has a perspective for future development in the interdisciplinary synergy of philosophy, physical-mathematical elaboration, experimental biology and clinical investigations.",
publisher = "Heidelberg: Springer Nature",
journal = "Biologia Futura",
title = "Biologia Futura: does the aging process contribute to the relativity of time?",
number = "74, 1-2",
doi = "10.1007/s42977-023-00167-2",
pages = "137-143"
}

Ajdžanović, V., Šošić-Jurjević, B., Ranin, J.,& Filipović, B.. (2023). Biologia Futura: does the aging process contribute to the relativity of time?. in Biologia Futura
Heidelberg: Springer Nature.(74), 137-143.
https://doi.org/10.1007/s42977-023-00167-2

Ajdžanović V, Šošić-Jurjević B, Ranin J, Filipović B. Biologia Futura: does the aging process contribute to the relativity of time?. in Biologia Futura. 2023;(74):137-143.
doi:10.1007/s42977-023-00167-2 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Ranin, Jovan, Filipović, Branko, "Biologia Futura: does the aging process contribute to the relativity of time?" in Biologia Futura, no. 74 (2023):137-143,
https://doi.org/10.1007/s42977-023-00167-2 . .

TY  - CONF
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Manojlović-Stojanoski, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6695
AB  - Развој организма и хомеостатски механизми се прилагођавају променљивим условима спољашње средине још током пренаталног периода. Женски репродуктивни систем активно реагује адаптирајући развој и диференцирање гонадотропних ћелија хипофизе и оваријума у складу са условима околине.1,2 Различити типови стреса, укључујући хормонске, нутритивне и психолошке изазове могу проузроковати бројне промене које обликују будући репродуктивни капацитет женке и заправо су део пренаталног програмирања физиологије организма. Третман гравидних женки дексаметазоном (Дк) значајно утиче на развој фетуса и опонаша антенаталну терапију гравидних жена глукокортикоидима као најчешће присутни третман у обстетричкој пракси. Стога су гравидне женке пацова Дк третиране (3 x 0,5 мг/кг/тм Дк) од 16. до 18. дана гестације. Пренатално Дк излагање изазвало је смањење масе фетуса непосредно пред рођење. Забележено је значајно смањење апсолутног броја ФСХ (фоликулостимулирајући хормон) и ЛХ (лутеинизирајући хормон) гонадотропних ћелија хипофизе (p < 0,05) од феталног, преко постнаталног, до пуберталног периода у односу на контролне вредности. Паралелно, Дк излагање током феталног развоја проузроковало је смањење волумена оваријума код потомака старих 16 и 38 дана (p < 0,05). Број фоликула је смањен код 16 дана старих потомака, док су поремећај процеса фоликулогенезе и одложен почетак пубертета забележени код потомака старих 38 дана. Можемо закључити да пренатални период развоја снажно утиче на фертилитет женки током репродуктивног периода.
AB  - Razvoj organizma i homeostatski mehanizmi se prilagođavaju promenljivim uslovima spoljašnje sredine još tokom prenatalnog perioda. Ženski reproduktivni sistem aktivno reaguje adaptirajući razvoj i diferenciranje gonadotropnih ćelija hipofize i ovarijuma u skladu sa uslovima okoline.1,2 Različiti tipovi stresa, uključujući hormonske, nutritivne i psihološke izazove mogu prouzrokovati brojne promene koje oblikuju budući reproduktivni kapacitet ženke i zapravo su deo prenatalnog programiranja fiziologije organizma. Tretman gravidnih ženki deksametazonom (Dk) značajno utiče na razvoj fetusa i oponaša antenatalnu terapiju gravidnih žena glukokortikoidima kao najčešće prisutni tretman u obstetričkoj praksi. Stoga su gravidne ženke pacova Dk tretirane (3 x 0,5 mg/kg/tm Dk) od 16. do 18. dana gestacije. Prenatalno Dk izlaganje izazvalo je smanjenje mase fetusa neposredno pred rođenje. Zabeleženo je značajno smanjenje apsolutnog broja FSH (folikulostimulirajući hormon) i LH (luteinizirajući hormon) gonadotropnih ćelija hipofize (p < 0,05) od fetalnog, preko postnatalnog, do pubertalnog perioda u odnosu na kontrolne vrednosti. Paralelno, Dk izlaganje tokom fetalnog razvoja prouzrokovalo je smanjenje volumena ovarijuma kod potomaka starih 16 i 38 dana (p < 0,05). Broj folikula je smanjen kod 16 dana starih potomaka, dok su poremećaj procesa folikulogeneze i odložen početak puberteta zabeleženi kod potomaka starih 38 dana. Možemo zaključiti da prenatalni period razvoja snažno utiče na fertilitet ženki tokom reproduktivnog perioda.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Развој женског репродуктивног система и последице пренаталног излагања дексаметазону
T1  - Razvoj ženskog reproduktivnog sistema i posledice prenatalnog izlaganja deksametazonu
SP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6695
ER  - 

@conference{
author = "Ristić, Nataša and Nestorović, Nataša and Trifunović, Svetlana and Šošić-Jurjević, Branka and Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Miler, Marko and Manojlović-Stojanoski, Milica",
year = "2022",
abstract = "Развој организма и хомеостатски механизми се прилагођавају променљивим условима спољашње средине још током пренаталног периода. Женски репродуктивни систем активно реагује адаптирајући развој и диференцирање гонадотропних ћелија хипофизе и оваријума у складу са условима околине.1,2 Различити типови стреса, укључујући хормонске, нутритивне и психолошке изазове могу проузроковати бројне промене које обликују будући репродуктивни капацитет женке и заправо су део пренаталног програмирања физиологије организма. Третман гравидних женки дексаметазоном (Дк) значајно утиче на развој фетуса и опонаша антенаталну терапију гравидних жена глукокортикоидима као најчешће присутни третман у обстетричкој пракси. Стога су гравидне женке пацова Дк третиране (3 x 0,5 мг/кг/тм Дк) од 16. до 18. дана гестације. Пренатално Дк излагање изазвало је смањење масе фетуса непосредно пред рођење. Забележено је значајно смањење апсолутног броја ФСХ (фоликулостимулирајући хормон) и ЛХ (лутеинизирајући хормон) гонадотропних ћелија хипофизе (p < 0,05) од феталног, преко постнаталног, до пуберталног периода у односу на контролне вредности. Паралелно, Дк излагање током феталног развоја проузроковало је смањење волумена оваријума код потомака старих 16 и 38 дана (p < 0,05). Број фоликула је смањен код 16 дана старих потомака, док су поремећај процеса фоликулогенезе и одложен почетак пубертета забележени код потомака старих 38 дана. Можемо закључити да пренатални период развоја снажно утиче на фертилитет женки током репродуктивног периода., Razvoj organizma i homeostatski mehanizmi se prilagođavaju promenljivim uslovima spoljašnje sredine još tokom prenatalnog perioda. Ženski reproduktivni sistem aktivno reaguje adaptirajući razvoj i diferenciranje gonadotropnih ćelija hipofize i ovarijuma u skladu sa uslovima okoline.1,2 Različiti tipovi stresa, uključujući hormonske, nutritivne i psihološke izazove mogu prouzrokovati brojne promene koje oblikuju budući reproduktivni kapacitet ženke i zapravo su deo prenatalnog programiranja fiziologije organizma. Tretman gravidnih ženki deksametazonom (Dk) značajno utiče na razvoj fetusa i oponaša antenatalnu terapiju gravidnih žena glukokortikoidima kao najčešće prisutni tretman u obstetričkoj praksi. Stoga su gravidne ženke pacova Dk tretirane (3 x 0,5 mg/kg/tm Dk) od 16. do 18. dana gestacije. Prenatalno Dk izlaganje izazvalo je smanjenje mase fetusa neposredno pred rođenje. Zabeleženo je značajno smanjenje apsolutnog broja FSH (folikulostimulirajući hormon) i LH (luteinizirajući hormon) gonadotropnih ćelija hipofize (p < 0,05) od fetalnog, preko postnatalnog, do pubertalnog perioda u odnosu na kontrolne vrednosti. Paralelno, Dk izlaganje tokom fetalnog razvoja prouzrokovalo je smanjenje volumena ovarijuma kod potomaka starih 16 i 38 dana (p < 0,05). Broj folikula je smanjen kod 16 dana starih potomaka, dok su poremećaj procesa folikulogeneze i odložen početak puberteta zabeleženi kod potomaka starih 38 dana. Možemo zaključiti da prenatalni period razvoja snažno utiče na fertilitet ženki tokom reproduktivnog perioda.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Развој женског репродуктивног система и последице пренаталног излагања дексаметазону, Razvoj ženskog reproduktivnog sistema i posledice prenatalnog izlaganja deksametazonu",
pages = "379",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6695"
}

Ristić, N., Nestorović, N., Trifunović, S., Šošić-Jurjević, B., Filipović, B., Ajdžanović, V., Živanović, J., Miler, M.,& Manojlović-Stojanoski, M.. (2022). Развој женског репродуктивног система и последице пренаталног излагања дексаметазону. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 379.
https://hdl.handle.net/21.15107/rcub_ibiss_6695

Ristić N, Nestorović N, Trifunović S, Šošić-Jurjević B, Filipović B, Ajdžanović V, Živanović J, Miler M, Manojlović-Stojanoski M. Развој женског репродуктивног система и последице пренаталног излагања дексаметазону. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:379.
https://hdl.handle.net/21.15107/rcub_ibiss_6695 .

Ristić, Nataša, Nestorović, Nataša, Trifunović, Svetlana, Šošić-Jurjević, Branka, Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Miler, Marko, Manojlović-Stojanoski, Milica, "Развој женског репродуктивног система и последице пренаталног излагања дексаметазону" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):379,
https://hdl.handle.net/21.15107/rcub_ibiss_6695 .

TY  - JOUR
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Jovanović, Ivan
AU  - Kundalić, Braca
AU  - Pavlović, Miljana
AU  - Graovac, Ivana
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Marković-Filipović, Jelena
AU  - Milošević, Verica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4978
AB  - Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction.
AB  - Увод/Циљ У овој студији смо указали на имунохистоморфометријске карактеристике соматотропних (ГХ) и фоликулостелатних (ФС) ћелија хипофизе човека током старења. Методе На хистолошким пресецима хипофизе 14 мушких кадавера различите старости, ГХ и ФС ћелије су имунохистохемијски обележене одговарајућим антителима (моноклонско анти-ГХ антитело и поликлонско анти-С100 антитело). Имунопозитивне ГХ- и ФС-ћелије су морфометријски анализиране коришћењем ImageJ софтвера. Резултати Добијени резултати морфометријске анализе су показали да се површина коју заузимају ГХ ћелије значајно повећава са старењем. У овим ћелијама нуклеарно-цитоплазматски однос се постепено смањивао и након 70. године старости постао значајно већи. Волуменска густина ГХ ћелија се није мењала током старења, док је код ФС ћелија овај параметар значајно повећан у случајевима старијим од 70 година. Нуклеарно-цитоплазматски однос ГХ ћелија је у негативној корелацији са волуменском густином ФС ћелија. Закључак На основу добијених резултата, закључили смо да се хипертрофија ГХ и ФС ћелија јавља код мушкараца са старењем и да корелација између морфометријских параметара ова два типа ћелија указује на њихову међусобну интеракцију.
PB  - Belgrade: Serbian Medical Society
T2  - Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine
T1  - Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing
T1  - Морфометријска анализа соматотропних и фоликулостелатних ћелија
аденохипофизе човека током старења
IS  - 5-6
VL  - 150
DO  - 10.2298/SARH211214044C
SP  - 274
EP  - 280
ER  - 

@article{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Jovanović, Ivan and Kundalić, Braca and Pavlović, Miljana and Graovac, Ivana and Ajdžanović, Vladimir and Ristić, Nataša and Marković-Filipović, Jelena and Milošević, Verica",
year = "2022",
abstract = "Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction., Увод/Циљ У овој студији смо указали на имунохистоморфометријске карактеристике соматотропних (ГХ) и фоликулостелатних (ФС) ћелија хипофизе човека током старења. Методе На хистолошким пресецима хипофизе 14 мушких кадавера различите старости, ГХ и ФС ћелије су имунохистохемијски обележене одговарајућим антителима (моноклонско анти-ГХ антитело и поликлонско анти-С100 антитело). Имунопозитивне ГХ- и ФС-ћелије су морфометријски анализиране коришћењем ImageJ софтвера. Резултати Добијени резултати морфометријске анализе су показали да се површина коју заузимају ГХ ћелије значајно повећава са старењем. У овим ћелијама нуклеарно-цитоплазматски однос се постепено смањивао и након 70. године старости постао значајно већи. Волуменска густина ГХ ћелија се није мењала током старења, док је код ФС ћелија овај параметар значајно повећан у случајевима старијим од 70 година. Нуклеарно-цитоплазматски однос ГХ ћелија је у негативној корелацији са волуменском густином ФС ћелија. Закључак На основу добијених резултата, закључили смо да се хипертрофија ГХ и ФС ћелија јавља код мушкараца са старењем и да корелација између морфометријских параметара ова два типа ћелија указује на њихову међусобну интеракцију.",
publisher = "Belgrade: Serbian Medical Society",
journal = "Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine",
title = "Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing, Морфометријска анализа соматотропних и фоликулостелатних ћелија
аденохипофизе човека током старења",
number = "5-6",
volume = "150",
doi = "10.2298/SARH211214044C",
pages = "274-280"
}

Čukuranović-Kokoris, J., Đorđević, M., Jovanović, I., Kundalić, B., Pavlović, M., Graovac, I., Ajdžanović, V., Ristić, N., Marković-Filipović, J.,& Milošević, V.. (2022). Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing. in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine
Belgrade: Serbian Medical Society., 150(5-6), 274-280.
https://doi.org/10.2298/SARH211214044C

Čukuranović-Kokoris J, Đorđević M, Jovanović I, Kundalić B, Pavlović M, Graovac I, Ajdžanović V, Ristić N, Marković-Filipović J, Milošević V. Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing. in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine. 2022;150(5-6):274-280.
doi:10.2298/SARH211214044C .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Jovanović, Ivan, Kundalić, Braca, Pavlović, Miljana, Graovac, Ivana, Ajdžanović, Vladimir, Ristić, Nataša, Marković-Filipović, Jelena, Milošević, Verica, "Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing" in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine, 150, no. 5-6 (2022):274-280,
https://doi.org/10.2298/SARH211214044C . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Trifunović, Svetlana
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4971
AB  - Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.
PB  - New York: Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats
DO  - 10.1017/S1431927622000721
ER  - 

@article{
author = "Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Trifunović, Svetlana and Ristić, Nataša and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.",
publisher = "New York: Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats",
doi = "10.1017/S1431927622000721"
}

Filipović, B., Ajdžanović, V., Živanović, J., Trifunović, S., Ristić, N., Milošević, V.,& Šošić-Jurjević, B.. (2022). Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats. in Microscopy and Microanalysis
New York: Cambridge University Press..
https://doi.org/10.1017/S1431927622000721

Filipović B, Ajdžanović V, Živanović J, Trifunović S, Ristić N, Milošević V, Šošić-Jurjević B. Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats. in Microscopy and Microanalysis. 2022;.
doi:10.1017/S1431927622000721 .

Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Trifunović, Svetlana, Ristić, Nataša, Milošević, Verica, Šošić-Jurjević, Branka , "Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats" in Microscopy and Microanalysis (2022),
https://doi.org/10.1017/S1431927622000721 . .

TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Dovenska, Monika
AU  - Popovska-Perčinić, Florina
PY  - 2022
UR  - https://www.sciendo.com/article/10.2478/acve-2022-0001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4965
AB  - Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.
T2  - Acta Veterinaria
T1  - The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study
IS  - 1
VL  - 72
DO  - 10.2478/acve-2022-0001
SP  - 1
EP  - 15
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Ajdžanović, Vladimir and Pendovski, Lazo and Ristić, Nataša and Milošević, Verica and Dovenska, Monika and Popovska-Perčinić, Florina",
year = "2022",
abstract = "Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.",
journal = "Acta Veterinaria",
title = "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0001",
pages = "1-15"
}

Čukuranović Kokoris, J., Ajdžanović, V., Pendovski, L., Ristić, N., Milošević, V., Dovenska, M.,& Popovska-Perčinić, F.. (2022). The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria, 72(1), 1-15.
https://doi.org/10.2478/acve-2022-0001

Čukuranović Kokoris J, Ajdžanović V, Pendovski L, Ristić N, Milošević V, Dovenska M, Popovska-Perčinić F. The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria. 2022;72(1):1-15.
doi:10.2478/acve-2022-0001 .

Čukuranović Kokoris, Jovana, Ajdžanović, Vladimir, Pendovski, Lazo, Ristić, Nataša, Milošević, Verica, Dovenska, Monika, Popovska-Perčinić, Florina, "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study" in Acta Veterinaria, 72, no. 1 (2022):1-15,
https://doi.org/10.2478/acve-2022-0001 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Trifunović, Svetlana
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Ristić, Nataša
AU  - Filipović, Branko
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/2/791
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35054977
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4769
AB  - Vitamin D plays an essential role in prevention and treatment of osteoporosis. Thyroid hormones, in addition to vitamin D, significantly contribute to regulation of bone remodeling cycle and health. There is currently no data about a possible connection between vitamin D treatment and the thyroid in the context of osteoporosis. Middle-aged Wistar rats were divided into: sham operated (SO), orchidectomized (Orx), and cholecalciferol-treated orchidectomized (Orx + Vit. D3; 5 µg/kg b.m./day during three weeks) groups (n = 6/group). Concentration of 25(OH)D in serum of the Orx + Vit. D3 group increased 4 and 3.2 times (p < 0.0001) respectively, compared to Orx and SO group. T4, TSH, and calcitonin in serum remained unaltered. Vit. D3 treatment induced changes in thyroid functional morphology that indicate increased utilization of stored colloid and release of thyroid hormones in comparison with hormone synthesis, to maintain hormonal balance. Increased expression of nuclear VDR (p < 0.05) points to direct, TSH independent action of Vit. D on thyrocytes. Strong CYP24A1 immunostaining in C cells suggests its prominent expression in response to Vit. D in this cell subpopulation in orchidectomized rat model of osteoporosis. The indirect effect of Vit. D on bone, through fine regulation of thyroid function, is small.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.
IS  - 2
VL  - 23
DO  - 10.3390/ijms23020791
SP  - 791
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Trifunović, Svetlana and Živanović, Jasmina and Ajdžanović, Vladimir and Miler, Marko and Ristić, Nataša and Filipović, Branko",
year = "2022",
abstract = "Vitamin D plays an essential role in prevention and treatment of osteoporosis. Thyroid hormones, in addition to vitamin D, significantly contribute to regulation of bone remodeling cycle and health. There is currently no data about a possible connection between vitamin D treatment and the thyroid in the context of osteoporosis. Middle-aged Wistar rats were divided into: sham operated (SO), orchidectomized (Orx), and cholecalciferol-treated orchidectomized (Orx + Vit. D3; 5 µg/kg b.m./day during three weeks) groups (n = 6/group). Concentration of 25(OH)D in serum of the Orx + Vit. D3 group increased 4 and 3.2 times (p < 0.0001) respectively, compared to Orx and SO group. T4, TSH, and calcitonin in serum remained unaltered. Vit. D3 treatment induced changes in thyroid functional morphology that indicate increased utilization of stored colloid and release of thyroid hormones in comparison with hormone synthesis, to maintain hormonal balance. Increased expression of nuclear VDR (p < 0.05) points to direct, TSH independent action of Vit. D on thyrocytes. Strong CYP24A1 immunostaining in C cells suggests its prominent expression in response to Vit. D in this cell subpopulation in orchidectomized rat model of osteoporosis. The indirect effect of Vit. D on bone, through fine regulation of thyroid function, is small.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.",
number = "2",
volume = "23",
doi = "10.3390/ijms23020791",
pages = "791"
}

Šošić-Jurjević, B., Trifunović, S., Živanović, J., Ajdžanović, V., Miler, M., Ristić, N.,& Filipović, B.. (2022). Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.. in International Journal of Molecular Sciences
Basel: MDPI., 23(2), 791.
https://doi.org/10.3390/ijms23020791

Šošić-Jurjević B, Trifunović S, Živanović J, Ajdžanović V, Miler M, Ristić N, Filipović B. Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.. in International Journal of Molecular Sciences. 2022;23(2):791.
doi:10.3390/ijms23020791 .

Šošić-Jurjević, Branka , Trifunović, Svetlana, Živanović, Jasmina, Ajdžanović, Vladimir, Miler, Marko, Ristić, Nataša, Filipović, Branko, "Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis." in International Journal of Molecular Sciences, 23, no. 2 (2022):791,
https://doi.org/10.3390/ijms23020791 . .

TY  - JOUR
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Stanković, Sanja
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S094096022100162X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4499
AB  - Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.
PB  - Elsevier GmbH
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause
VL  - 239
DO  - 10.1016/j.aanat.2021.151836
SP  - 151836
ER  - 

@article{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Miler, Marko and Stanković, Sanja and Milošević, Verica and Filipović, Branko",
year = "2022",
abstract = "Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.",
publisher = "Elsevier GmbH",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause",
volume = "239",
doi = "10.1016/j.aanat.2021.151836",
pages = "151836"
}

Živanović, J., Jarić, I., Ajdžanović, V., Miler, M., Stanković, S., Milošević, V.,& Filipović, B.. (2022). Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH., 239, 151836.
https://doi.org/10.1016/j.aanat.2021.151836

Živanović J, Jarić I, Ajdžanović V, Miler M, Stanković S, Milošević V, Filipović B. Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger. 2022;239:151836.
doi:10.1016/j.aanat.2021.151836 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Miler, Marko, Stanković, Sanja, Milošević, Verica, Filipović, Branko, "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause" in Annals of Anatomy - Anatomischer Anzeiger, 239 (2022):151836,
https://doi.org/10.1016/j.aanat.2021.151836 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Vodnik, Vesna
AU  - Filipović, Branko
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5076
PB  - Dortmund: Leibniz Research Centre for Working Environment and Human Factors, EXCLI Journal
T2  - EXCLI Journal
T1  - Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment
VL  - 21
DO  - 10.17179/excli2022-5130
SP  - 941
EP  - 947
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Vodnik, Vesna and Filipović, Branko",
year = "2022",
publisher = "Dortmund: Leibniz Research Centre for Working Environment and Human Factors, EXCLI Journal",
journal = "EXCLI Journal",
title = "Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment",
volume = "21",
doi = "10.17179/excli2022-5130",
pages = "941-947"
}

Ajdžanović, V., Šošić-Jurjević, B., Vodnik, V.,& Filipović, B.. (2022). Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment. in EXCLI Journal
Dortmund: Leibniz Research Centre for Working Environment and Human Factors, EXCLI Journal., 21, 941-947.
https://doi.org/10.17179/excli2022-5130

Ajdžanović V, Šošić-Jurjević B, Vodnik V, Filipović B. Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment. in EXCLI Journal. 2022;21:941-947.
doi:10.17179/excli2022-5130 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Vodnik, Vesna, Filipović, Branko, "Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment" in EXCLI Journal, 21 (2022):941-947,
https://doi.org/10.17179/excli2022-5130 . .

TY  - CONF
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka 
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6034
AB  - Старење доводи до акумулације и смањене елиминације реактивних врста,
стварајући оксидациону средину у јетри. Једна од могућих опција за одлагање овог
догађаја је употреба антиоксиданата из цитруса у склопу процеса здравог старења.
Стога, ова студија je имала за циљ да истражи ефекте ериоцитрина (ЕРИ),
полифенола из лимуна, на редокс средину у јетри код 24-месечних мужјака Wistar
пацова. ЕРИ (30 мг/кг т.м.) је даван орално, једном дневно током четири недеље.
Контролне групе су примиле или носач третмана (сунцокретово уље) или су остале
интактне. Примењена методологија подразумевала је имуноблот и qPCR анализу,
детекцију нивоа персулфидације у гелу и биохемијске анализе. Третман ЕРИ
повећао је експресију гена и протеина за нуклеарни фактор 2 (Nrf2), тиоредоксин
(Trx) 1, глутатион пероксидазу и редуктазу. Експресија гена супероксид дисмутазе
2 (SOD2) и Trx2 је смањена, док је примена ЕРИ повећала експресију протеина
SOD2. Штавише, ЕРИ је смањио концентрацију малондиалдехида, маркера
оксидативног оштећења у ћелији. Показали смо смањење персулфидације протеина
у јетри након третмана ЕРИ, док су генска и протеинска експресија ензима који
производе H2S остале непромењене. У закључку, ЕРИ појачава регулацију редокс
регулатора јетре Nrf2, који активира антиоксидативни одбрамбени систем и Trx1,
што доводи до смањења нивоа персулфидације протеина у јетри. Наши налази
указују на потенцијал ЕРИ у унапређењу редокс средине у јетри старих пацова.
AB  - Starenje dovodi do akumulacije i smanjene eliminacije reaktivnih vrsta,
stvarajući oksidacionu sredinu u jetri. Jedna od mogućih opcija za odlaganje ovog
događaja je upotreba antioksidanata iz citrusa u sklopu procesa zdravog starenja.
Stoga, ova studija je imala za cilj da istraži efekte eriocitrina (ERI),
polifenola iz limuna, na redoks sredinu u jetri kod 24-mesečnih mužjaka Wistar
pacova. ERI (30 mg/kg t.m.) je davan oralno, jednom dnevno tokom četiri nedelje.
Kontrolne grupe su primile ili nosač tretmana (suncokretovo ulje) ili su ostale
intaktne. Primenjena metodologija podrazumevala je imunoblot i qPCR analizu,
detekciju nivoa persulfidacije u gelu i biohemijske analize. Tretman ERI
povećao je ekspresiju gena i proteina za nuklearni faktor 2 (Nrf2), tioredoksin
(Trx) 1, glutation peroksidazu i reduktazu. Ekspresija gena superoksid dismutaze
2 (SOD2) i Trx2 je smanjena, dok je primena ERI povećala ekspresiju proteina
SOD2. Štaviše, ERI je smanjio koncentraciju malondialdehida, markera
oksidativnog oštećenja u ćeliji. Pokazali smo smanjenje persulfidacije proteina
u jetri nakon tretmana ERI, dok su genska i proteinska ekspresija enzima koji
proizvode H2S ostale nepromenjene. U zaključku, ERI pojačava regulaciju redoks
regulatora jetre Nrf2, koji aktivira antioksidativni odbrambeni sistem i Trx1,
što dovodi do smanjenja nivoa persulfidacije proteina u jetri. Naši nalazi
ukazuju na potencijal ERI u unapređenju redoks sredine u jetri starih pacova.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова
T1  - Eriocitrin, polifenol iz limuna, unapređuje redoks sredinu u jetri starih pacova
SP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6034
ER  - 

@conference{
author = "Miler, Marko and Živanović, Jasmina and Ristić, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Šošić-Jurjević, Branka  and Nestorović, Nataša and Filipović, Branko and Ajdžanović, Vladimir",
year = "2022",
abstract = "Старење доводи до акумулације и смањене елиминације реактивних врста,
стварајући оксидациону средину у јетри. Једна од могућих опција за одлагање овог
догађаја је употреба антиоксиданата из цитруса у склопу процеса здравог старења.
Стога, ова студија je имала за циљ да истражи ефекте ериоцитрина (ЕРИ),
полифенола из лимуна, на редокс средину у јетри код 24-месечних мужјака Wistar
пацова. ЕРИ (30 мг/кг т.м.) је даван орално, једном дневно током четири недеље.
Контролне групе су примиле или носач третмана (сунцокретово уље) или су остале
интактне. Примењена методологија подразумевала је имуноблот и qPCR анализу,
детекцију нивоа персулфидације у гелу и биохемијске анализе. Третман ЕРИ
повећао је експресију гена и протеина за нуклеарни фактор 2 (Nrf2), тиоредоксин
(Trx) 1, глутатион пероксидазу и редуктазу. Експресија гена супероксид дисмутазе
2 (SOD2) и Trx2 је смањена, док је примена ЕРИ повећала експресију протеина
SOD2. Штавише, ЕРИ је смањио концентрацију малондиалдехида, маркера
оксидативног оштећења у ћелији. Показали смо смањење персулфидације протеина
у јетри након третмана ЕРИ, док су генска и протеинска експресија ензима који
производе H2S остале непромењене. У закључку, ЕРИ појачава регулацију редокс
регулатора јетре Nrf2, који активира антиоксидативни одбрамбени систем и Trx1,
што доводи до смањења нивоа персулфидације протеина у јетри. Наши налази
указују на потенцијал ЕРИ у унапређењу редокс средине у јетри старих пацова., Starenje dovodi do akumulacije i smanjene eliminacije reaktivnih vrsta,
stvarajući oksidacionu sredinu u jetri. Jedna od mogućih opcija za odlaganje ovog
događaja je upotreba antioksidanata iz citrusa u sklopu procesa zdravog starenja.
Stoga, ova studija je imala za cilj da istraži efekte eriocitrina (ERI),
polifenola iz limuna, na redoks sredinu u jetri kod 24-mesečnih mužjaka Wistar
pacova. ERI (30 mg/kg t.m.) je davan oralno, jednom dnevno tokom četiri nedelje.
Kontrolne grupe su primile ili nosač tretmana (suncokretovo ulje) ili su ostale
intaktne. Primenjena metodologija podrazumevala je imunoblot i qPCR analizu,
detekciju nivoa persulfidacije u gelu i biohemijske analize. Tretman ERI
povećao je ekspresiju gena i proteina za nuklearni faktor 2 (Nrf2), tioredoksin
(Trx) 1, glutation peroksidazu i reduktazu. Ekspresija gena superoksid dismutaze
2 (SOD2) i Trx2 je smanjena, dok je primena ERI povećala ekspresiju proteina
SOD2. Štaviše, ERI je smanjio koncentraciju malondialdehida, markera
oksidativnog oštećenja u ćeliji. Pokazali smo smanjenje persulfidacije proteina
u jetri nakon tretmana ERI, dok su genska i proteinska ekspresija enzima koji
proizvode H2S ostale nepromenjene. U zaključku, ERI pojačava regulaciju redoks
regulatora jetre Nrf2, koji aktivira antioksidativni odbrambeni sistem i Trx1,
što dovodi do smanjenja nivoa persulfidacije proteina u jetri. Naši nalazi
ukazuju na potencijal ERI u unapređenju redoks sredine u jetri starih pacova.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова, Eriocitrin, polifenol iz limuna, unapređuje redoks sredinu u jetri starih pacova",
pages = "374",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6034"
}

Miler, M., Živanović, J., Ristić, N., Manojlović-Stojanoski, M., Trifunović, S., Šošić-Jurjević, B., Nestorović, N., Filipović, B.,& Ajdžanović, V.. (2022). Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 374.
https://hdl.handle.net/21.15107/rcub_ibiss_6034

Miler M, Živanović J, Ristić N, Manojlović-Stojanoski M, Trifunović S, Šošić-Jurjević B, Nestorović N, Filipović B, Ajdžanović V. Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:374.
https://hdl.handle.net/21.15107/rcub_ibiss_6034 .

Miler, Marko, Živanović, Jasmina, Ristić, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Šošić-Jurjević, Branka , Nestorović, Nataša, Filipović, Branko, Ajdžanović, Vladimir, "Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):374,
https://hdl.handle.net/21.15107/rcub_ibiss_6034 .

TY  - CONF
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Nestorović, Nataša
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4976
AB  - Thyroid C-cells, as a second type of endocrine cell population within thyroid, produce and secrete peptide hormone calcitonin (CT). This hypocalcemic hormone acts as an inhibitor of bone resorption. The aging is a complex process that alters various cellular functions. Therefore, the aim of this study was to examine the aging-related changes in the structure and function of CT-producing thyroid C-cells in male Wistar rats, using histomorphometric, ultrastructural and biochemical analysis. The investigation was performed on three groups of male rats: adult (3-months old), middle-aged (16-months old) and old (24-months old). The peroxidase-antiperoxidase method was applied for localization of CT in the C-cells. Stereological analysis was performed using Olimpus microscope (BX-51), equipped with a microcator, a motorised stage and a CCD video camera, and controlled by the newCAST stereological software package. Blood serum samples were analyzed for determination of CT, testosterone (T), calcium (Ca2+) and phosphorus (P) concentrations. We found a significant increase in the volume density (Vv) of thyroid C-cells in both middle-aged and old rats, all compared to adult animals. The percentage of smaller volume range C-cells (<500 μm3) increases, while the proportion of greater volume rang C-cells (both 500-1000 μm3 and >1000 μm3) markedly decreases with aging. By ultrastructural analysis we found that the average number of secretory granules per C-cell was significantly increased in both middle-aged and aged rats, all compared to adults. Unlike the C-cells of adult rats, these granules in older, especially in old animals, had a content of fairly low density. By the biochemical analysis, we detected a significant increase in serum CT levels, while serum T was markedly reduced in both middle aged and old rats, all in comparison with adults. Serum Ca2+ concentration significantly decreased in middle-aged rats compared to adults, while concentration of serum Pwas lower in both middle-aged and old rats, all related to adult group. Our findings show that aging process increases the Vv of thyroid C-cells, with a simultaneous change in percentage of cells with larger and smaller volume rang, and an increase in the number of both cell types. These changes, accompanied by modulation of the cellular ultrastructure and an increase in serum CT levels, reflect the structure and function of CT-producing thyroid C-cells in our aging model.
PB  - bioscientifica
C3  - European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy
T1  - The effects of aging on thyroid C-cells in male rats
DO  - 10.1530/endoabs.81.P477
SP  - P477
ER  - 

@conference{
author = "Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Nestorović, Nataša and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Thyroid C-cells, as a second type of endocrine cell population within thyroid, produce and secrete peptide hormone calcitonin (CT). This hypocalcemic hormone acts as an inhibitor of bone resorption. The aging is a complex process that alters various cellular functions. Therefore, the aim of this study was to examine the aging-related changes in the structure and function of CT-producing thyroid C-cells in male Wistar rats, using histomorphometric, ultrastructural and biochemical analysis. The investigation was performed on three groups of male rats: adult (3-months old), middle-aged (16-months old) and old (24-months old). The peroxidase-antiperoxidase method was applied for localization of CT in the C-cells. Stereological analysis was performed using Olimpus microscope (BX-51), equipped with a microcator, a motorised stage and a CCD video camera, and controlled by the newCAST stereological software package. Blood serum samples were analyzed for determination of CT, testosterone (T), calcium (Ca2+) and phosphorus (P) concentrations. We found a significant increase in the volume density (Vv) of thyroid C-cells in both middle-aged and old rats, all compared to adult animals. The percentage of smaller volume range C-cells (<500 μm3) increases, while the proportion of greater volume rang C-cells (both 500-1000 μm3 and >1000 μm3) markedly decreases with aging. By ultrastructural analysis we found that the average number of secretory granules per C-cell was significantly increased in both middle-aged and aged rats, all compared to adults. Unlike the C-cells of adult rats, these granules in older, especially in old animals, had a content of fairly low density. By the biochemical analysis, we detected a significant increase in serum CT levels, while serum T was markedly reduced in both middle aged and old rats, all in comparison with adults. Serum Ca2+ concentration significantly decreased in middle-aged rats compared to adults, while concentration of serum Pwas lower in both middle-aged and old rats, all related to adult group. Our findings show that aging process increases the Vv of thyroid C-cells, with a simultaneous change in percentage of cells with larger and smaller volume rang, and an increase in the number of both cell types. These changes, accompanied by modulation of the cellular ultrastructure and an increase in serum CT levels, reflect the structure and function of CT-producing thyroid C-cells in our aging model.",
publisher = "bioscientifica",
journal = "European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy",
title = "The effects of aging on thyroid C-cells in male rats",
doi = "10.1530/endoabs.81.P477",
pages = "P477"
}

Filipović, B., Ajdžanović, V., Živanović, J., Manojlović-Stojanoski, M., Trifunović, S., Nestorović, N.,& Šošić-Jurjević, B.. (2022). The effects of aging on thyroid C-cells in male rats. in European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy
bioscientifica., P477.
https://doi.org/10.1530/endoabs.81.P477

Filipović B, Ajdžanović V, Živanović J, Manojlović-Stojanoski M, Trifunović S, Nestorović N, Šošić-Jurjević B. The effects of aging on thyroid C-cells in male rats. in European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy. 2022;:P477.
doi:10.1530/endoabs.81.P477 .

Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Nestorović, Nataša, Šošić-Jurjević, Branka , "The effects of aging on thyroid C-cells in male rats" in European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy (2022):P477,
https://doi.org/10.1530/endoabs.81.P477 . .

TY  - CONF
AU  - Šošić-Jurjević, Branka 
AU  - Jovanović, Ljubiša
AU  - Marina, Ljiljana
AU  - Ristić, Nataša
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Luetjohann, Dieter
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4975
AB  - The cholesterol oxidation product 27-hydroxycholesterol (27OHC) is enzymatically
produced from cholesterol by CYP27A1 in an alternative pathway of
cholesterol degradation to bile acids. This oxysterol also acts as an endogenous
selective estrogen receptor modulator (SERM). In healthy humans its
concentration in circulation increases in hypercholesterolemia and with age,
and is associated with increased risk of atherosclerosis, cardiovascular diseases
and breast cancer. Several drugs with SERM activity used for treatments of breast
cancer or osteoporosis have been reported to have sporadic hepatotoxic effects.
Women suffer from some liver diseases more commonly (acute liver failure,
autoimmune hepatitis, benign liver lesions, or primary biliary cirrhosis). For all of
these the incidence increases with advancing age. To the best of our knowledge,
there is no information in the literature, clinical or experimental, relating changes
in hepatic 27OHC with incidence of liver disease in the context of aging and sex.
To address this problem, we examined the effect of age and sex on liver and serum
concentrations of 27OHC, as well as the immunostaining pattern of CYP27A1 in
the liver of four-month and 24-month-old Wistar rats (experiments were repeated
twice with similar results, nZ5-6 animals/group) using LC MS/MS and
immunohistochemistry, respectively. Furthermore, we examined changes in
total cholesterol and concentration in liver and serum, liver histopathology, as
well as serum concentration of hepatic enzymes, alanine (ALT) and aspartate
aminotransferase (AST). The effect of age (P!0.05) on increase of serum and
hepatic 27OHC was obtained both in males and females (P!0.05) and followed
the same pattern of age-related total cholesterol increase (P!0.05). However, the
intrahepatic increase of 27OHC was dramatically more pronounced only in oldaged
females (P!0.0001). CYP27A1 immunostaining intensity was similar in all
experimental groups, being the strongest in the cytoplasm of centrilobular
hepatocytes, but the immunopositivity was diffusely spread throughout the liver
lobule. Histopathological analysis revealed age-related hepatocellular degeneration
(swelling and hydropic degeneration, increased fraction of binuclear
hepatocytes and focal fatty changes) only in females. Moreover, age-related
elevation of alanine transaminase (ALT) was observed only in female rats (P!
0.01). In conclusion, the obtained results confirmed age-related female-specific
increase of hepatic 27OHC as well as hepatocyte degeneration obsereved only in
the liver of rat females. These age-related adaptive changes in cholesterol
metabolism may atenuate hepatoprotective estrogen-like effects in the liver.
PB  - bioscientifica
C3  - 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy
T1  - Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?
DO  - 10.1530/endoabs.81.P615
SP  - P615
ER  - 

@conference{
author = "Šošić-Jurjević, Branka  and Jovanović, Ljubiša and Marina, Ljiljana and Ristić, Nataša and Miler, Marko and Ajdžanović, Vladimir and Filipović, Branko and Luetjohann, Dieter",
year = "2022",
abstract = "The cholesterol oxidation product 27-hydroxycholesterol (27OHC) is enzymatically
produced from cholesterol by CYP27A1 in an alternative pathway of
cholesterol degradation to bile acids. This oxysterol also acts as an endogenous
selective estrogen receptor modulator (SERM). In healthy humans its
concentration in circulation increases in hypercholesterolemia and with age,
and is associated with increased risk of atherosclerosis, cardiovascular diseases
and breast cancer. Several drugs with SERM activity used for treatments of breast
cancer or osteoporosis have been reported to have sporadic hepatotoxic effects.
Women suffer from some liver diseases more commonly (acute liver failure,
autoimmune hepatitis, benign liver lesions, or primary biliary cirrhosis). For all of
these the incidence increases with advancing age. To the best of our knowledge,
there is no information in the literature, clinical or experimental, relating changes
in hepatic 27OHC with incidence of liver disease in the context of aging and sex.
To address this problem, we examined the effect of age and sex on liver and serum
concentrations of 27OHC, as well as the immunostaining pattern of CYP27A1 in
the liver of four-month and 24-month-old Wistar rats (experiments were repeated
twice with similar results, nZ5-6 animals/group) using LC MS/MS and
immunohistochemistry, respectively. Furthermore, we examined changes in
total cholesterol and concentration in liver and serum, liver histopathology, as
well as serum concentration of hepatic enzymes, alanine (ALT) and aspartate
aminotransferase (AST). The effect of age (P!0.05) on increase of serum and
hepatic 27OHC was obtained both in males and females (P!0.05) and followed
the same pattern of age-related total cholesterol increase (P!0.05). However, the
intrahepatic increase of 27OHC was dramatically more pronounced only in oldaged
females (P!0.0001). CYP27A1 immunostaining intensity was similar in all
experimental groups, being the strongest in the cytoplasm of centrilobular
hepatocytes, but the immunopositivity was diffusely spread throughout the liver
lobule. Histopathological analysis revealed age-related hepatocellular degeneration
(swelling and hydropic degeneration, increased fraction of binuclear
hepatocytes and focal fatty changes) only in females. Moreover, age-related
elevation of alanine transaminase (ALT) was observed only in female rats (P!
0.01). In conclusion, the obtained results confirmed age-related female-specific
increase of hepatic 27OHC as well as hepatocyte degeneration obsereved only in
the liver of rat females. These age-related adaptive changes in cholesterol
metabolism may atenuate hepatoprotective estrogen-like effects in the liver.",
publisher = "bioscientifica",
journal = "24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy",
title = "Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?",
doi = "10.1530/endoabs.81.P615",
pages = "P615"
}

Šošić-Jurjević, B., Jovanović, L., Marina, L., Ristić, N., Miler, M., Ajdžanović, V., Filipović, B.,& Luetjohann, D.. (2022). Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?. in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy
bioscientifica., P615.
https://doi.org/10.1530/endoabs.81.P615

Šošić-Jurjević B, Jovanović L, Marina L, Ristić N, Miler M, Ajdžanović V, Filipović B, Luetjohann D. Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?. in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy. 2022;:P615.
doi:10.1530/endoabs.81.P615 .

Šošić-Jurjević, Branka , Jovanović, Ljubiša, Marina, Ljiljana, Ristić, Nataša, Miler, Marko, Ajdžanović, Vladimir, Filipović, Branko, Luetjohann, Dieter, "Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?" in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy (2022):P615,
https://doi.org/10.1530/endoabs.81.P615 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia-Eirini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Harnafi, Hicham
AU  - Milošević, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenkovic, Dragan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4977
AB  - Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.
PB  - Elsevier B.V.
T2  - Ageing Research Reviews
T1  - Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies
VL  - 79:101649
DO  - 10.1016/j.arr.2022.101649
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia-Eirini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Harnafi, Hicham and Milošević, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenkovic, Dragan",
year = "2022",
abstract = "Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.",
publisher = "Elsevier B.V.",
journal = "Ageing Research Reviews",
title = "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies",
volume = "79:101649",
doi = "10.1016/j.arr.2022.101649"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Harnafi, H., Milošević, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenkovic, D.. (2022). Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews
Elsevier B.V.., 79:101649.
https://doi.org/10.1016/j.arr.2022.101649

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou G, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Harnafi H, Milošević V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenkovic D. Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews. 2022;79:101649.
doi:10.1016/j.arr.2022.101649 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia-Eirini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Harnafi, Hicham, Milošević, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenkovic, Dragan, "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies" in Ageing Research Reviews, 79:101649 (2022),
https://doi.org/10.1016/j.arr.2022.101649 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milenković, Dragan
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4144
AB  - In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.
PB  - Elsevir
T2  - Medical Hypotheses
T1  - Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design
VL  - 148
DO  - 10.1016/j.mehy.2021.110516
SP  - 110516
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka  and Filipović, Branko and Milenković, Dragan and Jakovljević, Vladimir and Milošević, Verica",
year = "2021",
abstract = "In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.",
publisher = "Elsevir",
journal = "Medical Hypotheses",
title = "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design",
volume = "148",
doi = "10.1016/j.mehy.2021.110516",
pages = "110516"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B., Filipović, B., Milenković, D., Jakovljević, V.,& Milošević, V.. (2021). Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses
Elsevir., 148, 110516.
https://doi.org/10.1016/j.mehy.2021.110516

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B, Milenković D, Jakovljević V, Milošević V. Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses. 2021;148:110516.
doi:10.1016/j.mehy.2021.110516 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka , Filipović, Branko, Milenković, Dragan, Jakovljević, Vladimir, Milošević, Verica, "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design" in Medical Hypotheses, 148 (2021):110516,
https://doi.org/10.1016/j.mehy.2021.110516 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Miler, Marko
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4223
PB  - Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors
T2  - EXCLI Journal
T1  - Margins of beneficial daily dosage of supplements in prevention of COVID-19
VL  - 20
DO  - 10.17179/excli2021-3790
SP  - 828
EP  - 834
ER  - 

@article{
author = "Ajdžanović, Vladimir and Filipović, Branko and Šošić-Jurjević, Branka  and Miler, Marko and Milošević, Verica",
year = "2021",
publisher = "Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors",
journal = "EXCLI Journal",
title = "Margins of beneficial daily dosage of supplements in prevention of COVID-19",
volume = "20",
doi = "10.17179/excli2021-3790",
pages = "828-834"
}

Ajdžanović, V., Filipović, B., Šošić-Jurjević, B., Miler, M.,& Milošević, V.. (2021). Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal
Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors., 20, 828-834.
https://doi.org/10.17179/excli2021-3790

Ajdžanović V, Filipović B, Šošić-Jurjević B, Miler M, Milošević V. Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal. 2021;20:828-834.
doi:10.17179/excli2021-3790 .

Ajdžanović, Vladimir, Filipović, Branko, Šošić-Jurjević, Branka , Miler, Marko, Milošević, Verica, "Margins of beneficial daily dosage of supplements in prevention of COVID-19" in EXCLI Journal, 20 (2021):828-834,
https://doi.org/10.17179/excli2021-3790 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2021
UR  - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/thyroid-gland-alterations-in-oldaged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study/4C35B9A6AFED404C65272C08C8C2DC27
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4143
AB  - The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.
PB  - Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
DO  - 10.1017/S1431927621000064
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Marković Filipović, Jelena and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2021",
abstract = "The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.",
publisher = "Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study",
doi = "10.1017/S1431927621000064"
}

Miler, M., Ajdžanović, V., Živanović, J., Marković Filipović, J., Šošić-Jurjević, B.,& Milošević, V.. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis
Cambridge University Press..
https://doi.org/10.1017/S1431927621000064

Miler M, Ajdžanović V, Živanović J, Marković Filipović J, Šošić-Jurjević B, Milošević V. Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis. 2021;.
doi:10.1017/S1431927621000064 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Marković Filipović, Jelena, Šošić-Jurjević, Branka , Milošević, Verica, "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study" in Microscopy and Microanalysis (2021),
https://doi.org/10.1017/S1431927621000064 . .

TY  - JOUR
AU  - Vodnik, Vesna V.
AU  - Mojić, Marija
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Ajdžanović, Vladimir
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Marković, Mirjana M.
AU  - Barudžija, Tanja
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4238
AB  - Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines
VL  - 124
DO  - 10.1016/j.msec.2021.112078
SP  - 112078
ER  - 

@article{
author = "Vodnik, Vesna V. and Mojić, Marija and Stamenović, Una and Otoničar, Mojca and Ajdžanović, Vladimir and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Marković, Mirjana M. and Barudžija, Tanja and Filipović, Branko and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2021",
abstract = "Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines",
volume = "124",
doi = "10.1016/j.msec.2021.112078",
pages = "112078"
}

Vodnik, V. V., Mojić, M., Stamenović, U., Otoničar, M., Ajdžanović, V., Maksimović-Ivanić, D., Mijatović, S., Marković, M. M., Barudžija, T., Filipović, B., Milošević, V.,& Šošić-Jurjević, B.. (2021). Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C
Elsevier Ltd., 124, 112078.
https://doi.org/10.1016/j.msec.2021.112078

Vodnik VV, Mojić M, Stamenović U, Otoničar M, Ajdžanović V, Maksimović-Ivanić D, Mijatović S, Marković MM, Barudžija T, Filipović B, Milošević V, Šošić-Jurjević B. Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C. 2021;124:112078.
doi:10.1016/j.msec.2021.112078 .

Vodnik, Vesna V., Mojić, Marija, Stamenović, Una, Otoničar, Mojca, Ajdžanović, Vladimir, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Marković, Mirjana M., Barudžija, Tanja, Filipović, Branko, Milošević, Verica, Šošić-Jurjević, Branka , "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines" in Materials Science and Engineering C, 124 (2021):112078,
https://doi.org/10.1016/j.msec.2021.112078 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2021
UR  - http://www.publish.csiro.au/?paper=RD20164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4153
AB  - Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.
PB  - CSIRO
T2  - Reproduction, Fertility and Development
T1  - Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat
IS  - 3
VL  - 33
DO  - 10.1071/RD20164
SP  - 245
EP  - 255
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2021",
abstract = "Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.",
publisher = "CSIRO",
journal = "Reproduction, Fertility and Development",
title = "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat",
number = "3",
volume = "33",
doi = "10.1071/RD20164",
pages = "245-255"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2021). Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development
CSIRO., 33(3), 245-255.
https://doi.org/10.1071/RD20164

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development. 2021;33(3):245-255.
doi:10.1071/RD20164 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat" in Reproduction, Fertility and Development, 33, no. 3 (2021):245-255,
https://doi.org/10.1071/RD20164 . .