Đorđević, Ana

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Authority KeyName Variants
orcid::0000-0002-7042-1631
  • Đorđević, Ana (84)
Projects
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200053 (University of Belgrade, Institute for Multidisciplinary Research) Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production
Swiss National Science Foundation, [Grant SCOPES JRP IZ73Z0_152331] Swiss National Science Foundation, Grant SCOPES JRP IZ73Z0_152331
Austrian Science Fund (FWF) project P32464 Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials
Analysis of the structural genome changes as a diagnostic and prognostic parameter of human diseases Effects of metabolic and nonmetabolic stressors on the expression and action of neuroendocrine regulators of energy homeostasis
Genetic basis of human vascular and inflammatory diseases Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200116 (University of Belgrade, Faculty of Agriculture) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200143 (University of Belgrade, Faculty of Veterinary Medicine)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200178 (University of Belgrade, Faculty of Biology) International Commission for the Protection of the Danube River (ICPDR)
SCOPES JRP (Grant no. IZ73ZO_152331) SCOPES JRP (Grant No IZ73ZO_152331)
SCOPES JRP. Grant Number: IZ73ZO_152331 SCOPES project No IZ73Z0_152331
Swiss National Science Foundation under Grant [SCOPES JRP IZ73Z0_152331] the bilateral project of Republic of Serbia and Republic of Austria 2019-2021(WTZ-SRB12-2018)
The bilateral project of the Republic of Serbia and the Republic of Austria 2019-2021 (WTZ-SRB12-2018), and the Austrian Science Fund (FWF) Project P32464. This study was founded by Austrian Science Fund (FWF) project P32464
This study was founded by the bilateral project of Republic of Serbia and Republic of Austria 2019-2021 (WTZ-SRB12-2018)

Author's Bibliography

Impact of untreated wastewaters on the microbiological water quality of the Danube River and its tributaries in Serbia

Kolarević, Stoimir; Micsinai, Adrienn; Szanto-Egesz, Reka; Lukacs, Alena; Kračun-Kolarević, Margareta; Jovanović Marić, Jovana; Đorđević, Ana; Vojnović-Milutinović, Danijela; Kirschner, Alexander; Farnleitner, Andreas; Linke, Rita; Đukić, Aleksandar; Kostić, Jovana; Sunjog, Karolina; Paunović, Momir

(Belgrade: Serbian Society for Microbiology, 2024)

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6693
AB  - Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.
PB  - Belgrade: Serbian Society for Microbiology
C3  - Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
T1  - Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia
SP  - 65
ER  - 
@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2024",
abstract = "Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia",
title = "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia",
pages = "65"
}
Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2024). Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
Belgrade: Serbian Society for Microbiology., 65.
Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia. 2024;:65..
Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia" in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia (2024):65.

Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet

Bursać, Biljana; Bellachioma, Luisa; Gligorovska, Ljupka; Jovanović, Mirna; Teofilović, Ana; Vratarić, Miloš; Vojnović Milutinović, Danijela; Albacete, Alfonso; Martínez-Melgarejo, Purificación A; Morresi, Camilla; Damiani, Elisabetta; Bacchetti, Tiziana; Đorđević, Ana

(Hoboken: John Wiley and Sons, 2024)

TY  - JOUR
AU  - Bursać, Biljana
AU  - Bellachioma, Luisa
AU  - Gligorovska, Ljupka
AU  - Jovanović, Mirna
AU  - Teofilović, Ana
AU  - Vratarić, Miloš
AU  - Vojnović Milutinović, Danijela
AU  - Albacete, Alfonso
AU  - Martínez-Melgarejo, Purificación A
AU  - Morresi, Camilla
AU  - Damiani, Elisabetta
AU  - Bacchetti, Tiziana
AU  - Đorđević, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6533
AB  - Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Crocus sativus (Cr) tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral (VAT) and subcutaneous (SAT) adipose tissue hypertrophy, and lipid metabolism in an animal model of diet-induced obesity. To this end, mouse 3T3-F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications.
PB  - Hoboken: John Wiley and Sons
T2  - BioFactors
T1  - Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet
DO  - 10.1002/biof.2043
ER  - 
@article{
author = "Bursać, Biljana and Bellachioma, Luisa and Gligorovska, Ljupka and Jovanović, Mirna and Teofilović, Ana and Vratarić, Miloš and Vojnović Milutinović, Danijela and Albacete, Alfonso and Martínez-Melgarejo, Purificación A and Morresi, Camilla and Damiani, Elisabetta and Bacchetti, Tiziana and Đorđević, Ana",
year = "2024",
abstract = "Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Crocus sativus (Cr) tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral (VAT) and subcutaneous (SAT) adipose tissue hypertrophy, and lipid metabolism in an animal model of diet-induced obesity. To this end, mouse 3T3-F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications.",
publisher = "Hoboken: John Wiley and Sons",
journal = "BioFactors",
title = "Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet",
doi = "10.1002/biof.2043"
}
Bursać, B., Bellachioma, L., Gligorovska, L., Jovanović, M., Teofilović, A., Vratarić, M., Vojnović Milutinović, D., Albacete, A., Martínez-Melgarejo, P. A., Morresi, C., Damiani, E., Bacchetti, T.,& Đorđević, A.. (2024). Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet. in BioFactors
Hoboken: John Wiley and Sons..
https://doi.org/10.1002/biof.2043
Bursać B, Bellachioma L, Gligorovska L, Jovanović M, Teofilović A, Vratarić M, Vojnović Milutinović D, Albacete A, Martínez-Melgarejo PA, Morresi C, Damiani E, Bacchetti T, Đorđević A. Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet. in BioFactors. 2024;.
doi:10.1002/biof.2043 .
Bursać, Biljana, Bellachioma, Luisa, Gligorovska, Ljupka, Jovanović, Mirna, Teofilović, Ana, Vratarić, Miloš, Vojnović Milutinović, Danijela, Albacete, Alfonso, Martínez-Melgarejo, Purificación A, Morresi, Camilla, Damiani, Elisabetta, Bacchetti, Tiziana, Đorđević, Ana, "Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet" in BioFactors (2024),
https://doi.org/10.1002/biof.2043 . .

Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet

Bursać, Biljana; Bellachioma, Luisa; Gligorovska, Ljupka; Jovanović, Mirna; Teofilović, Ana; Vratarić, Miloš; Vojnović Milutinović, Danijela; Albacete, Alfonso; Martínez-Melgarejo, Purificación A; Morresi, Camilla; Damiani, Elisabetta; Bacchetti, Tiziana; Đorđević, Ana

(Hoboken: John Wiley and Sons, 2024)

TY  - JOUR
AU  - Bursać, Biljana
AU  - Bellachioma, Luisa
AU  - Gligorovska, Ljupka
AU  - Jovanović, Mirna
AU  - Teofilović, Ana
AU  - Vratarić, Miloš
AU  - Vojnović Milutinović, Danijela
AU  - Albacete, Alfonso
AU  - Martínez-Melgarejo, Purificación A
AU  - Morresi, Camilla
AU  - Damiani, Elisabetta
AU  - Bacchetti, Tiziana
AU  - Đorđević, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6532
AB  - Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Crocus sativus (Cr) tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral (VAT) and subcutaneous (SAT) adipose tissue hypertrophy, and lipid metabolism in an animal model of diet-induced obesity. To this end, mouse 3T3-F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications.
PB  - Hoboken: John Wiley and Sons
T2  - BioFactors
T1  - Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet
DO  - 10.1002/biof.2043
ER  - 
@article{
author = "Bursać, Biljana and Bellachioma, Luisa and Gligorovska, Ljupka and Jovanović, Mirna and Teofilović, Ana and Vratarić, Miloš and Vojnović Milutinović, Danijela and Albacete, Alfonso and Martínez-Melgarejo, Purificación A and Morresi, Camilla and Damiani, Elisabetta and Bacchetti, Tiziana and Đorđević, Ana",
year = "2024",
abstract = "Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Crocus sativus (Cr) tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral (VAT) and subcutaneous (SAT) adipose tissue hypertrophy, and lipid metabolism in an animal model of diet-induced obesity. To this end, mouse 3T3-F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications.",
publisher = "Hoboken: John Wiley and Sons",
journal = "BioFactors",
title = "Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet",
doi = "10.1002/biof.2043"
}
Bursać, B., Bellachioma, L., Gligorovska, L., Jovanović, M., Teofilović, A., Vratarić, M., Vojnović Milutinović, D., Albacete, A., Martínez-Melgarejo, P. A., Morresi, C., Damiani, E., Bacchetti, T.,& Đorđević, A.. (2024). Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet. in BioFactors
Hoboken: John Wiley and Sons..
https://doi.org/10.1002/biof.2043
Bursać B, Bellachioma L, Gligorovska L, Jovanović M, Teofilović A, Vratarić M, Vojnović Milutinović D, Albacete A, Martínez-Melgarejo PA, Morresi C, Damiani E, Bacchetti T, Đorđević A. Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet. in BioFactors. 2024;.
doi:10.1002/biof.2043 .
Bursać, Biljana, Bellachioma, Luisa, Gligorovska, Ljupka, Jovanović, Mirna, Teofilović, Ana, Vratarić, Miloš, Vojnović Milutinović, Danijela, Albacete, Alfonso, Martínez-Melgarejo, Purificación A, Morresi, Camilla, Damiani, Elisabetta, Bacchetti, Tiziana, Đorđević, Ana, "Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet" in BioFactors (2024),
https://doi.org/10.1002/biof.2043 . .

Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia

Kolarević, Stoimir; Adrienn, Micsinai; Szanto-Egesz, Reka; Lukacs, Alena; Kračun-Kolarević, Margareta; Jovanović Marić, Jovana; Đorđević, Ana; Vojnović-Milutinović, Danijela; Kirschner, Alexander; Farnleitner, Andreas; Linke, Rita; Đukić, Aleksandar; Kostić, Jovana; Sunjog, Karolina; Paunović, Momir

(Austrian Committee - International Association for Danube Research, 2023)

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Adrienn, Micsinai
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6591
AB  - Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6591
ER  - 
@conference{
author = "Kolarević, Stoimir and Adrienn, Micsinai and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2023",
abstract = "Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia",
pages = "11",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6591"
}
Kolarević, S., Adrienn, M., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2023). Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591
Kolarević S, Adrienn M, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .
Kolarević, Stoimir, Adrienn, Micsinai, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):11,
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

Uticaj delecije gena Mif na razvoj gojaznosti i steatoze jetre kod miševa na režimu ishrane obogaćene fruktozom

Gligorovska, Ljupka; Đorđević, Ana

(Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 2023)

TY  - CHAP
AU  - Gligorovska, Ljupka
AU  - Đorđević, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6422
AB  - Hronična inflamacija niskog intenziteta ima važnu ulogu u patogenezi metaboličkih poremećaja, kao što su gojaznost i dislipidemija, koji su često izazvani ishranom obogaćenom fruktozom i predstavljaju faktore rizika za razvoj steatoze jetre i dijabetesa tipa 2. Faktor migracije makrofaga (MIF) je plejotropni citokin koji pored uloge u regulaciji imunskog odgovora može da reguliše metaboličke procese i oslobađanje insulina iz pankreasa, međutim njegova uloga u metaboličkoj inflamaciji nije još uvek dovoljno razjašnjena. Dodatno, pokazano je da se miševi sa deletiranim genom Mif odlikuju izmenjenom osetljivošću na insulin i glukozu. Stoga, fokus ovog rada je bio usmeren ka boljem razumevanju molekularnih mehanizama metaboličkih efekata MIF-a, kao i ka rasvetljavanju kontroverzne uloge ovog citokina u razvoju poremećaja lipidnog metabolizma u jetri i visceralnom masnom tkivu miševa na režimu ishrane obogaćene fruktozom.
AB  - Chronic low-grade inflammation plays an important role in the development of metabolic disorders such as obesity and dyslipidemia, which are often induced by a fructose-enriched diet and are risk factors for the development of hepatic steatosis and type 2 diabetes. Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that, in addition to its role in regulating the immune response, can regulate metabolic processes and the release of insulin from the pancreas; however its role in metabolic inflammation is not well understood. In addition, mice with a deleted Mif gene were found to have altered sensitivity to insulin and glucose. Therefore, the focus of this work was to better understand the molecular mechanisms of the metabolic effects of MIF and to elucidate the controversial role of this cytokine in the development ofdyslipidemia in the liver and visceral adipose tissue of mice on fructose-enriched diet.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in molecular biology
T1  - Uticaj delecije gena Mif na razvoj gojaznosti i steatoze jetre kod miševa na režimu ishrane obogaćene fruktozom
T1  - The effects of deletion of the Mif gene on the development of obesity and hepatic steatosis on mice on fructose enriched diet
IS  - 3
SP  - 151
EP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6422
ER  - 
@inbook{
author = "Gligorovska, Ljupka and Đorđević, Ana",
year = "2023",
abstract = "Hronična inflamacija niskog intenziteta ima važnu ulogu u patogenezi metaboličkih poremećaja, kao što su gojaznost i dislipidemija, koji su često izazvani ishranom obogaćenom fruktozom i predstavljaju faktore rizika za razvoj steatoze jetre i dijabetesa tipa 2. Faktor migracije makrofaga (MIF) je plejotropni citokin koji pored uloge u regulaciji imunskog odgovora može da reguliše metaboličke procese i oslobađanje insulina iz pankreasa, međutim njegova uloga u metaboličkoj inflamaciji nije još uvek dovoljno razjašnjena. Dodatno, pokazano je da se miševi sa deletiranim genom Mif odlikuju izmenjenom osetljivošću na insulin i glukozu. Stoga, fokus ovog rada je bio usmeren ka boljem razumevanju molekularnih mehanizama metaboličkih efekata MIF-a, kao i ka rasvetljavanju kontroverzne uloge ovog citokina u razvoju poremećaja lipidnog metabolizma u jetri i visceralnom masnom tkivu miševa na režimu ishrane obogaćene fruktozom., Chronic low-grade inflammation plays an important role in the development of metabolic disorders such as obesity and dyslipidemia, which are often induced by a fructose-enriched diet and are risk factors for the development of hepatic steatosis and type 2 diabetes. Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that, in addition to its role in regulating the immune response, can regulate metabolic processes and the release of insulin from the pancreas; however its role in metabolic inflammation is not well understood. In addition, mice with a deleted Mif gene were found to have altered sensitivity to insulin and glucose. Therefore, the focus of this work was to better understand the molecular mechanisms of the metabolic effects of MIF and to elucidate the controversial role of this cytokine in the development ofdyslipidemia in the liver and visceral adipose tissue of mice on fructose-enriched diet.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in molecular biology",
booktitle = "Uticaj delecije gena Mif na razvoj gojaznosti i steatoze jetre kod miševa na režimu ishrane obogaćene fruktozom, The effects of deletion of the Mif gene on the development of obesity and hepatic steatosis on mice on fructose enriched diet",
number = "3",
pages = "151-166",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6422"
}
Gligorovska, L.,& Đorđević, A.. (2023). Uticaj delecije gena Mif na razvoj gojaznosti i steatoze jetre kod miševa na režimu ishrane obogaćene fruktozom. in Trends in molecular biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(3), 151-166.
https://hdl.handle.net/21.15107/rcub_ibiss_6422
Gligorovska L, Đorđević A. Uticaj delecije gena Mif na razvoj gojaznosti i steatoze jetre kod miševa na režimu ishrane obogaćene fruktozom. in Trends in molecular biology. 2023;(3):151-166.
https://hdl.handle.net/21.15107/rcub_ibiss_6422 .
Gligorovska, Ljupka, Đorđević, Ana, "Uticaj delecije gena Mif na razvoj gojaznosti i steatoze jetre kod miševa na režimu ishrane obogaćene fruktozom" in Trends in molecular biology, no. 3 (2023):151-166,
https://hdl.handle.net/21.15107/rcub_ibiss_6422 .

Modulation of hepatic lipid metabolism in obesity-resistant mice on a high-fat diet

Vratarić, Miloš; Teofilović, Ana; Vojnović Milutinović, Danijela; Veličković, Nataša; Bursać, Biljana; Gligorovska, Ljupka; Mićić, Bojana; Jovanović, Mirna; Đorđević, Ana

(Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 2023)

TY  - CONF
AU  - Vratarić, Miloš
AU  - Teofilović, Ana
AU  - Vojnović Milutinović, Danijela
AU  - Veličković, Nataša
AU  - Bursać, Biljana
AU  - Gligorovska, Ljupka
AU  - Mićić, Bojana
AU  - Jovanović, Mirna
AU  - Đorđević, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6419
AB  - Introduction: High-fat diet primarily leads to obesity but it can also lead to obesity resistant (OR) phenotype
with various metabolic complications. Liver plays central role in modulating lipid metabolism in
response to dyslipidemia induced by adipose tissue hypertrophy. The aim of this study was to define key
regulatory points that adjust lipid metabolism in the liver of OR mice on high-fat diet (HFD).
Methods:Male C57BL/6J mice were divided into two groups: control group on normal diet (10 kcal% fat,
D12450J, Research Diets, USA) and HFD group (60 kcal% fat, D12492, Research Diets, USA). After 14 weeks,
mice on HFD were classified as obese or OR based on 30% difference in body weight gain compared
with controls. Liver sections were analyzed histologically, while alterations in hepatic lipid metabolism
were assessed by qPCR and Western blot.
Results: Although HFD restricted hepatic de novo lipogenesis, increased influx of free fatty acids (FFA)
led to accumulation of lipid droplets in the liver of obese mice. In OR mice, liver morphology was restored,
as was expression of insulin sensitive sterol regulatory element-binding protein 1c (SREBP-1c).
Level of FFA transporter CD36 was reduced, whereas higher expression of diacylglycerol acyltransferase
2 limited lipotoxicity in OR compared with obese mice. FFA β-oxidation remained unchanged in both
HFD groups.
Conclusion: Lower FFA input and reduced lipid storage and lipotoxicity in the liver of OR mice suggest
that dyslipidemic complications associated with obesity could be ameliorated by targeted modulation
of expression of FFA transporters and regulators of lipid droplet formation.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Modulation of hepatic lipid metabolism in obesity-resistant mice on a high-fat diet
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6419
ER  - 
@conference{
author = "Vratarić, Miloš and Teofilović, Ana and Vojnović Milutinović, Danijela and Veličković, Nataša and Bursać, Biljana and Gligorovska, Ljupka and Mićić, Bojana and Jovanović, Mirna and Đorđević, Ana",
year = "2023",
abstract = "Introduction: High-fat diet primarily leads to obesity but it can also lead to obesity resistant (OR) phenotype
with various metabolic complications. Liver plays central role in modulating lipid metabolism in
response to dyslipidemia induced by adipose tissue hypertrophy. The aim of this study was to define key
regulatory points that adjust lipid metabolism in the liver of OR mice on high-fat diet (HFD).
Methods:Male C57BL/6J mice were divided into two groups: control group on normal diet (10 kcal% fat,
D12450J, Research Diets, USA) and HFD group (60 kcal% fat, D12492, Research Diets, USA). After 14 weeks,
mice on HFD were classified as obese or OR based on 30% difference in body weight gain compared
with controls. Liver sections were analyzed histologically, while alterations in hepatic lipid metabolism
were assessed by qPCR and Western blot.
Results: Although HFD restricted hepatic de novo lipogenesis, increased influx of free fatty acids (FFA)
led to accumulation of lipid droplets in the liver of obese mice. In OR mice, liver morphology was restored,
as was expression of insulin sensitive sterol regulatory element-binding protein 1c (SREBP-1c).
Level of FFA transporter CD36 was reduced, whereas higher expression of diacylglycerol acyltransferase
2 limited lipotoxicity in OR compared with obese mice. FFA β-oxidation remained unchanged in both
HFD groups.
Conclusion: Lower FFA input and reduced lipid storage and lipotoxicity in the liver of OR mice suggest
that dyslipidemic complications associated with obesity could be ameliorated by targeted modulation
of expression of FFA transporters and regulators of lipid droplet formation.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Modulation of hepatic lipid metabolism in obesity-resistant mice on a high-fat diet",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6419"
}
Vratarić, M., Teofilović, A., Vojnović Milutinović, D., Veličković, N., Bursać, B., Gligorovska, L., Mićić, B., Jovanović, M.,& Đorđević, A.. (2023). Modulation of hepatic lipid metabolism in obesity-resistant mice on a high-fat diet. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 147.
https://hdl.handle.net/21.15107/rcub_ibiss_6419
Vratarić M, Teofilović A, Vojnović Milutinović D, Veličković N, Bursać B, Gligorovska L, Mićić B, Jovanović M, Đorđević A. Modulation of hepatic lipid metabolism in obesity-resistant mice on a high-fat diet. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:147.
https://hdl.handle.net/21.15107/rcub_ibiss_6419 .
Vratarić, Miloš, Teofilović, Ana, Vojnović Milutinović, Danijela, Veličković, Nataša, Bursać, Biljana, Gligorovska, Ljupka, Mićić, Bojana, Jovanović, Mirna, Đorđević, Ana, "Modulation of hepatic lipid metabolism in obesity-resistant mice on a high-fat diet" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):147,
https://hdl.handle.net/21.15107/rcub_ibiss_6419 .

Metabolic disturbances in animal model of polycystic ovary syndrome: impact of early postnatal overfeeding

Mićić, Bojana; Veličković, Nataša; Đorđević, Ana; Teofilović, Ana; Kovačević, Sanja; Radovanović, Marina; Brkljačić, Jelena; Macut Djuro; Vojnović Milutinović, Danijela

(Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 2023)

TY  - CONF
AU  - Mićić, Bojana
AU  - Veličković, Nataša
AU  - Đorđević, Ana
AU  - Teofilović, Ana
AU  - Kovačević, Sanja
AU  - Radovanović, Marina
AU  - Brkljačić, Jelena
AU  - Macut Djuro
AU  - Vojnović Milutinović, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6418
AB  - Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects women’s
fertility and metabolic health throughout their life time. Insulin resistance and obesity, in conjunction
with excess androgens, are undeniably involved in its development. We aimed to elucidate how hyperandrogenemia
and prepubertal adiposity contribute to the development of metabolic disturbances in
rat model of PCOS.
Methods: The animal model of PCOS induced by 5a-dihydrotestosterone (DHT) was additionally challenged
by litter size reduction (LSR) during suckling period, to ensure overfeeding and development of
prepubertal adiposity. Systemic parameters of insulin sensitivity, along with markers of energy sensing,
insulin signaling, and lipid metabolism were analyzed in visceral adipose tissue (VAT) and skeletal muscle.
Results: The combination of treatments led to hyperinsulinemia and impaired systemic insulin sensitivity.
This was not accompanied with altered insulin signaling in the VAT, in spite of observed adipocytes
hypertrophy probably due to activation of AMPK and restrained lipogenesis in this tissue. On the other
hand, insulin signaling in skeletal muscle was impaired, which resulted in increased muscle fatty acid
uptake and oxidation after combined treatment. The switch to fatty acids oxidation subsequently led to
oxidative stress and inflammation, which was followed by adaptive activation of AMPK and increased
expression of its targets involved in antioxidant protection and mitochondrial biogenesis.
Conclusion: Our results suggest that prepubertal weight gain predisposes to insulin resistance development
in androgen-excess PCOS. The protective activation of AMPK in VAT and muscle makes it a potential
therapeutic target for insulin-resistant PCOS patients.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Metabolic disturbances in animal model of polycystic ovary syndrome: impact of early postnatal overfeeding
SP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6418
ER  - 
@conference{
author = "Mićić, Bojana and Veličković, Nataša and Đorđević, Ana and Teofilović, Ana and Kovačević, Sanja and Radovanović, Marina and Brkljačić, Jelena and Macut Djuro and Vojnović Milutinović, Danijela",
year = "2023",
abstract = "Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects women’s
fertility and metabolic health throughout their life time. Insulin resistance and obesity, in conjunction
with excess androgens, are undeniably involved in its development. We aimed to elucidate how hyperandrogenemia
and prepubertal adiposity contribute to the development of metabolic disturbances in
rat model of PCOS.
Methods: The animal model of PCOS induced by 5a-dihydrotestosterone (DHT) was additionally challenged
by litter size reduction (LSR) during suckling period, to ensure overfeeding and development of
prepubertal adiposity. Systemic parameters of insulin sensitivity, along with markers of energy sensing,
insulin signaling, and lipid metabolism were analyzed in visceral adipose tissue (VAT) and skeletal muscle.
Results: The combination of treatments led to hyperinsulinemia and impaired systemic insulin sensitivity.
This was not accompanied with altered insulin signaling in the VAT, in spite of observed adipocytes
hypertrophy probably due to activation of AMPK and restrained lipogenesis in this tissue. On the other
hand, insulin signaling in skeletal muscle was impaired, which resulted in increased muscle fatty acid
uptake and oxidation after combined treatment. The switch to fatty acids oxidation subsequently led to
oxidative stress and inflammation, which was followed by adaptive activation of AMPK and increased
expression of its targets involved in antioxidant protection and mitochondrial biogenesis.
Conclusion: Our results suggest that prepubertal weight gain predisposes to insulin resistance development
in androgen-excess PCOS. The protective activation of AMPK in VAT and muscle makes it a potential
therapeutic target for insulin-resistant PCOS patients.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Metabolic disturbances in animal model of polycystic ovary syndrome: impact of early postnatal overfeeding",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6418"
}
Mićić, B., Veličković, N., Đorđević, A., Teofilović, A., Kovačević, S., Radovanović, M., Brkljačić, J., Macut Djuro,& Vojnović Milutinović, D.. (2023). Metabolic disturbances in animal model of polycystic ovary syndrome: impact of early postnatal overfeeding. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 144.
https://hdl.handle.net/21.15107/rcub_ibiss_6418
Mićić B, Veličković N, Đorđević A, Teofilović A, Kovačević S, Radovanović M, Brkljačić J, Macut Djuro, Vojnović Milutinović D. Metabolic disturbances in animal model of polycystic ovary syndrome: impact of early postnatal overfeeding. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:144.
https://hdl.handle.net/21.15107/rcub_ibiss_6418 .
Mićić, Bojana, Veličković, Nataša, Đorđević, Ana, Teofilović, Ana, Kovačević, Sanja, Radovanović, Marina, Brkljačić, Jelena, Macut Djuro, Vojnović Milutinović, Danijela, "Metabolic disturbances in animal model of polycystic ovary syndrome: impact of early postnatal overfeeding" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):144,
https://hdl.handle.net/21.15107/rcub_ibiss_6418 .

Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes

Jovanović, Mirna; Milosavljević, Dragica; Dragišić Maksimović, Jelena; Maksimović, Vuk; Milivojević, Jasminka; Đorđević, Ana; Brkljačić, Jelena

(Belgrade: Faculty of Chemistry, 2023)

TY  - CONF
AU  - Jovanović, Mirna
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6238
AB  - Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.
PB  - Belgrade: Faculty of Chemistry
C3  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes
SP  - 131
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6238
ER  - 
@conference{
author = "Jovanović, Mirna and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka and Đorđević, Ana and Brkljačić, Jelena",
year = "2023",
abstract = "Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes",
pages = "131-132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6238"
}
Jovanović, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J., Đorđević, A.,& Brkljačić, J.. (2023). Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238
Jovanović M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević J, Đorđević A, Brkljačić J. Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .
Jovanović, Mirna, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka, Đorđević, Ana, Brkljačić, Jelena, "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023):131-132,
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .

AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding

Mićić, Bojana; Đorđević, Ana; Veličković, Nataša; Kovačević, Sanja; Martić, Teodora; Macut, Đuro; Vojnović-Milutinović, Danijela

(Basel: MDPI, 2023)

TY  - JOUR
AU  - Mićić, Bojana
AU  - Đorđević, Ana
AU  - Veličković, Nataša
AU  - Kovačević, Sanja
AU  - Martić, Teodora
AU  - Macut, Đuro
AU  - Vojnović-Milutinović, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5785
AB  - Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women of reproductive
age, often associated with obesity and insulin resistance. Childhood obesity is an important
predisposing factor for the development of PCOS later in life. Being particularly interested in the
interplay between prepubertal obesity and hyperandrogenemia, we investigated the effects of early
postnatal overfeeding, accomplished by reducing litter size during the period of suckling, on energy
sensing and insulin signaling pathways in the gastrocnemius muscle of a rat model of PCOS-induced
by 5 -dihydrotestosterone (DHT). The combination of overfeeding and DHT treatment caused hyperinsulinemia
and decreased systemic insulin sensitivity. Early postnatal overfeeding induced defects
at critical nodes of the insulin signaling pathway in skeletal muscle, which was associated with
reduced glucose uptake in the presence of hyperandrogenemia. In this setting, under a combination
of overfeeding and DHT treatment, skeletal muscle switched to mitochondrial  -oxidation of fatty
acids, resulting in oxidative stress and inflammation that stimulated AMP-activated protein kinase
(AMPK) activity and its downstream targets involved in mitochondrial biogenesis and antioxidant
protection. Overall, a combination of overfeeding and hyperandrogenemia resulted in a prooxidative
and insulin-resistant state in skeletal muscle. This was accompanied by the activation of AMPK,
which could represent a potential therapeutic target in insulin-resistant PCOS patients.
PB  - Basel: MDPI
T2  - Biomedicines
T1  - AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding
IS  - 6
VL  - 11
DO  - 10.3390/biomedicines11061586
SP  - 1586
ER  - 
@article{
author = "Mićić, Bojana and Đorđević, Ana and Veličković, Nataša and Kovačević, Sanja and Martić, Teodora and Macut, Đuro and Vojnović-Milutinović, Danijela",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women of reproductive
age, often associated with obesity and insulin resistance. Childhood obesity is an important
predisposing factor for the development of PCOS later in life. Being particularly interested in the
interplay between prepubertal obesity and hyperandrogenemia, we investigated the effects of early
postnatal overfeeding, accomplished by reducing litter size during the period of suckling, on energy
sensing and insulin signaling pathways in the gastrocnemius muscle of a rat model of PCOS-induced
by 5 -dihydrotestosterone (DHT). The combination of overfeeding and DHT treatment caused hyperinsulinemia
and decreased systemic insulin sensitivity. Early postnatal overfeeding induced defects
at critical nodes of the insulin signaling pathway in skeletal muscle, which was associated with
reduced glucose uptake in the presence of hyperandrogenemia. In this setting, under a combination
of overfeeding and DHT treatment, skeletal muscle switched to mitochondrial  -oxidation of fatty
acids, resulting in oxidative stress and inflammation that stimulated AMP-activated protein kinase
(AMPK) activity and its downstream targets involved in mitochondrial biogenesis and antioxidant
protection. Overall, a combination of overfeeding and hyperandrogenemia resulted in a prooxidative
and insulin-resistant state in skeletal muscle. This was accompanied by the activation of AMPK,
which could represent a potential therapeutic target in insulin-resistant PCOS patients.",
publisher = "Basel: MDPI",
journal = "Biomedicines",
title = "AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding",
number = "6",
volume = "11",
doi = "10.3390/biomedicines11061586",
pages = "1586"
}
Mićić, B., Đorđević, A., Veličković, N., Kovačević, S., Martić, T., Macut, Đ.,& Vojnović-Milutinović, D.. (2023). AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding. in Biomedicines
Basel: MDPI., 11(6), 1586.
https://doi.org/10.3390/biomedicines11061586
Mićić B, Đorđević A, Veličković N, Kovačević S, Martić T, Macut Đ, Vojnović-Milutinović D. AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding. in Biomedicines. 2023;11(6):1586.
doi:10.3390/biomedicines11061586 .
Mićić, Bojana, Đorđević, Ana, Veličković, Nataša, Kovačević, Sanja, Martić, Teodora, Macut, Đuro, Vojnović-Milutinović, Danijela, "AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding" in Biomedicines, 11, no. 6 (2023):1586,
https://doi.org/10.3390/biomedicines11061586 . .
2

Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome

Ignjatović, Đurđica; Tovilović-Kovačević, Gordana; Mićić, Bojana; Tomić, Mirko; Đorđević, Ana; Macut, Đuro; Vojnović-Milutinović, Danijela

(Amsterdam: Elsevier, 2023)

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Mićić, Bojana
AU  - Tomić, Mirko
AU  - Đorđević, Ana
AU  - Macut, Đuro
AU  - Vojnović-Milutinović, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5821
AB  - Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increasein phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.
PB  - Amsterdam: Elsevier
T2  - Hormones and Behavior
T1  - Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome
VL  - 153
DO  - 10.1016/j.yhbeh.2023.105392
SP  - 105392
ER  - 
@article{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Mićić, Bojana and Tomić, Mirko and Đorđević, Ana and Macut, Đuro and Vojnović-Milutinović, Danijela",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increasein phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.",
publisher = "Amsterdam: Elsevier",
journal = "Hormones and Behavior",
title = "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome",
volume = "153",
doi = "10.1016/j.yhbeh.2023.105392",
pages = "105392"
}
Ignjatović, Đ., Tovilović-Kovačević, G., Mićić, B., Tomić, M., Đorđević, A., Macut, Đ.,& Vojnović-Milutinović, D.. (2023). Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior
Amsterdam: Elsevier., 153, 105392.
https://doi.org/10.1016/j.yhbeh.2023.105392
Ignjatović Đ, Tovilović-Kovačević G, Mićić B, Tomić M, Đorđević A, Macut Đ, Vojnović-Milutinović D. Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior. 2023;153:105392.
doi:10.1016/j.yhbeh.2023.105392 .
Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Mićić, Bojana, Tomić, Mirko, Đorđević, Ana, Macut, Đuro, Vojnović-Milutinović, Danijela, "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome" in Hormones and Behavior, 153 (2023):105392,
https://doi.org/10.1016/j.yhbeh.2023.105392 . .
6
1

Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome

Ignjatović, Đurđica; Tovilović-Kovačević, Gordana; Mićić, Bojana; Tomić, Mirko; Đorđević, Ana; Macut, Đuro; Vojnović-Milutinović, Danijela

(Amsterdam: Elsevier, 2023)

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Mićić, Bojana
AU  - Tomić, Mirko
AU  - Đorđević, Ana
AU  - Macut, Đuro
AU  - Vojnović-Milutinović, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5820
AB  - Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increase
in phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.
PB  - Amsterdam: Elsevier
T2  - Hormones and Behavior
T1  - Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome
VL  - 153
DO  - 10.1016/j.yhbeh.2023.105392
SP  - 105392
ER  - 
@article{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Mićić, Bojana and Tomić, Mirko and Đorđević, Ana and Macut, Đuro and Vojnović-Milutinović, Danijela",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increase
in phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.",
publisher = "Amsterdam: Elsevier",
journal = "Hormones and Behavior",
title = "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome",
volume = "153",
doi = "10.1016/j.yhbeh.2023.105392",
pages = "105392"
}
Ignjatović, Đ., Tovilović-Kovačević, G., Mićić, B., Tomić, M., Đorđević, A., Macut, Đ.,& Vojnović-Milutinović, D.. (2023). Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior
Amsterdam: Elsevier., 153, 105392.
https://doi.org/10.1016/j.yhbeh.2023.105392
Ignjatović Đ, Tovilović-Kovačević G, Mićić B, Tomić M, Đorđević A, Macut Đ, Vojnović-Milutinović D. Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior. 2023;153:105392.
doi:10.1016/j.yhbeh.2023.105392 .
Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Mićić, Bojana, Tomić, Mirko, Đorđević, Ana, Macut, Đuro, Vojnović-Milutinović, Danijela, "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome" in Hormones and Behavior, 153 (2023):105392,
https://doi.org/10.1016/j.yhbeh.2023.105392 . .
6
1
1

Oxidative Stress Linking Obesity and Cancer: Is Obesity a ‘Radical Trigger’ to Cancer?

Jovanović, Mirna; Kovačević, Sanja; Brkljačić, Jelena; Đorđević, Ana

(Basel: MDPI, 2023)

TY  - JOUR
AU  - Jovanović, Mirna
AU  - Kovačević, Sanja
AU  - Brkljačić, Jelena
AU  - Đorđević, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5756
AB  - Obesity is on the rise worldwide, and consequently, obesity-related non-communicable diseases are as well. Nutritional overload induces metabolic adaptations in an attempt to restore the disturbed balance, and the byproducts of the mechanisms at hand include an increased generation of reactive species. Obesity-related oxidative stress causes damage to vulnerable systems and ultimately contributes to neoplastic transformation. Dysfunctional obese adipose tissue releases cytokines and induces changes in the cell microenvironment, promoting cell survival and progression of the transformed cancer cells. Other than the increased risk of cancer development, obese cancer patients experience higher mortality rates and reduced therapy efficiency as well. The fact that obesity is considered the second leading preventable cause of cancer prioritizes the research on the mechanisms connecting obesity to cancerogenesis and finding the solutions to break the link. Oxidative stress is integral at different stages of cancer development and advancement in obese patients. Hypocaloric, balanced nutrition, and structured physical activity are some tools for relieving this burden. However, the sensitivity of simultaneously treating cancer and obesity poses a challenge. Further research on the obesity–cancer liaison would offer new perspectives on prevention programs and treatment development.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Oxidative Stress Linking Obesity and Cancer: Is Obesity a ‘Radical Trigger’ to Cancer?
IS  - 9
VL  - 24
DO  - 10.3390/ijms24098452
SP  - 8452
ER  - 
@article{
author = "Jovanović, Mirna and Kovačević, Sanja and Brkljačić, Jelena and Đorđević, Ana",
year = "2023",
abstract = "Obesity is on the rise worldwide, and consequently, obesity-related non-communicable diseases are as well. Nutritional overload induces metabolic adaptations in an attempt to restore the disturbed balance, and the byproducts of the mechanisms at hand include an increased generation of reactive species. Obesity-related oxidative stress causes damage to vulnerable systems and ultimately contributes to neoplastic transformation. Dysfunctional obese adipose tissue releases cytokines and induces changes in the cell microenvironment, promoting cell survival and progression of the transformed cancer cells. Other than the increased risk of cancer development, obese cancer patients experience higher mortality rates and reduced therapy efficiency as well. The fact that obesity is considered the second leading preventable cause of cancer prioritizes the research on the mechanisms connecting obesity to cancerogenesis and finding the solutions to break the link. Oxidative stress is integral at different stages of cancer development and advancement in obese patients. Hypocaloric, balanced nutrition, and structured physical activity are some tools for relieving this burden. However, the sensitivity of simultaneously treating cancer and obesity poses a challenge. Further research on the obesity–cancer liaison would offer new perspectives on prevention programs and treatment development.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Oxidative Stress Linking Obesity and Cancer: Is Obesity a ‘Radical Trigger’ to Cancer?",
number = "9",
volume = "24",
doi = "10.3390/ijms24098452",
pages = "8452"
}
Jovanović, M., Kovačević, S., Brkljačić, J.,& Đorđević, A.. (2023). Oxidative Stress Linking Obesity and Cancer: Is Obesity a ‘Radical Trigger’ to Cancer?. in International Journal of Molecular Sciences
Basel: MDPI., 24(9), 8452.
https://doi.org/10.3390/ijms24098452
Jovanović M, Kovačević S, Brkljačić J, Đorđević A. Oxidative Stress Linking Obesity and Cancer: Is Obesity a ‘Radical Trigger’ to Cancer?. in International Journal of Molecular Sciences. 2023;24(9):8452.
doi:10.3390/ijms24098452 .
Jovanović, Mirna, Kovačević, Sanja, Brkljačić, Jelena, Đorđević, Ana, "Oxidative Stress Linking Obesity and Cancer: Is Obesity a ‘Radical Trigger’ to Cancer?" in International Journal of Molecular Sciences, 24, no. 9 (2023):8452,
https://doi.org/10.3390/ijms24098452 . .
1
3
4

Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters

Kolarević, Stoimir; Micsinai, Adrienn; Szántó-Egész, Réka; Lukács, Alena; Kračun-Kolarević, Margareta; Đorđević, Ana; Jovanović Marić, Jovana; Vojnović-Milutinović, Danijela; Kirschner, Alexander K.T.; Farnleitner, Andreas H.; Linke, Rita; Đukić, Aleksandar; Kostić, Jovana; Sunjog, Karolina; Paunović, Momir

(Belgrade: Serbian Society of Microbiology, 2022)

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander K.T.
AU  - Farnleitner, Andreas H.
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6235
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
EP  - 615
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6235
ER  - 
@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Jovanović Marić, Jovana and Vojnović-Milutinović, Danijela and Kirschner, Alexander K.T. and Farnleitner, Andreas H. and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614-615",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6235"
}
Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Jovanović Marić, J., Vojnović-Milutinović, D., Kirschner, A. K.T., Farnleitner, A. H., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235
Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Jovanović Marić J, Vojnović-Milutinović D, Kirschner AK, Farnleitner AH, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .
Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Jovanović Marić, Jovana, Vojnović-Milutinović, Danijela, Kirschner, Alexander K.T., Farnleitner, Andreas H., Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):614-615,
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate

Dobrijević, Zorana; Matijašević-Joković, Suzana; Branković, Ana; Đorđević, Ana; Popović, Milica; Brajušković, Goran

(Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 2022)

TY  - CHAP
AU  - Dobrijević, Zorana
AU  - Matijašević-Joković, Suzana
AU  - Branković, Ana
AU  - Đorđević, Ana
AU  - Popović, Milica
AU  - Brajušković, Goran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6565
AB  - U ovom radu preglednog tipa prikazano je desetogodišnje iskustvo istraživačkog tima PRO-
STATSERBIA koji za temu svog istraživanja ima molekularnu osnovu karcinoma prostate (KP). Centar
za humanu molekularnu genetiku Biološkog fakulteta Univerziteta u Beogradu poseduje kolekciju
uzoraka i banku podataka za gotovo 1000 muškaraca sa bolestima prostate (KP i benigna hiperpla-
zija prostate) i preko 350 muškaraca bez znakova bolesti prostate iz populacije Republike Srbije. Naj-
veći broj studija bio je dizajniran kao studije asocijacije odabranih genetičkih varijanti sa rizikom za
razvoj i progresiju KP. U ovim studijama slučajeva i kontola ispitivane su genetičke varijente kako u ge-
nima za proteine i nekodirajuće molekule RNK, tako i u nekodirajućim regionima genoma (tzv. „gen-
skim pustinjama“). Pored studija slučajeva i kontrola, sprovođene su i meta-analize kao i analize
statističkih epistatičkih interakcija analiziranih genetičkih varijanti. Krajnji cilj ovih studija je kreiranje
algoritma za procenu rizika za progresiju bolesti koji bi se koristio u fazi aktivnog praćenja bolesnika
sa ranodijagnostikovanim KP. U poslednje vreme, istraživanja su usmerena na egzozome i njihov ma-
kromolekulski sadržaj (proteine i nekodirajuće molekule RNK) kao potencijalne biološke markere
tečne biopsije KP. Pored toga, biološki makromolekuli na površini egzozoma predstavljaju i ciljane
molekule u novim strategijama lečenja KP.
AB  - This review-type paper will present the ten-year experience of the PROSTATSERBIA research team,
which has the molecular basis of prostate cancer (PCa) as its research topic. The Center for Human Mo-
lecular Genetics of the Faculty of Biology, University of Belgrade has a collection of samples and a
data bank for almost 1000 men with prostate diseases (PCa and benign prostatic hyperplasia) and for
over 350 men without signs of prostate diseases, all from Serbian population. Most of the studies
were designed as studies of association of selected genetic variants with the risk for development
and progression of PCa. In these case-control studies, genetic variants were examined both in genes
for proteins and non-coding RNAs, as well as in non-coding regions of the genome (so-called gene
deserts). In addition to case control studies, both meta-analyses and the analysis of statistical epista-
tic interactions of the analyzed genetic variants were conducted. The main goal of all these studies was
to create an algorithm for risk assessment for disease progression that would be used in the phase of
active monitoring of patients with early diagnosed PCa. Recently, we shifted our research focus on
exosomes and their macromolecular content (proteins and microRNAs) as potential biological mar-
kers of liquid prostate cancer biopsy. In addition, macromolecules at the exosome surface represent
target molecules for new PCa treatment strategies.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in molecular biology
T1  - Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate
T1  - Modern approaches in research of the molecular basis of prostate cancer
IS  - 2
SP  - 63
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6565
ER  - 
@inbook{
author = "Dobrijević, Zorana and Matijašević-Joković, Suzana and Branković, Ana and Đorđević, Ana and Popović, Milica and Brajušković, Goran",
year = "2022",
abstract = "U ovom radu preglednog tipa prikazano je desetogodišnje iskustvo istraživačkog tima PRO-
STATSERBIA koji za temu svog istraživanja ima molekularnu osnovu karcinoma prostate (KP). Centar
za humanu molekularnu genetiku Biološkog fakulteta Univerziteta u Beogradu poseduje kolekciju
uzoraka i banku podataka za gotovo 1000 muškaraca sa bolestima prostate (KP i benigna hiperpla-
zija prostate) i preko 350 muškaraca bez znakova bolesti prostate iz populacije Republike Srbije. Naj-
veći broj studija bio je dizajniran kao studije asocijacije odabranih genetičkih varijanti sa rizikom za
razvoj i progresiju KP. U ovim studijama slučajeva i kontola ispitivane su genetičke varijente kako u ge-
nima za proteine i nekodirajuće molekule RNK, tako i u nekodirajućim regionima genoma (tzv. „gen-
skim pustinjama“). Pored studija slučajeva i kontrola, sprovođene su i meta-analize kao i analize
statističkih epistatičkih interakcija analiziranih genetičkih varijanti. Krajnji cilj ovih studija je kreiranje
algoritma za procenu rizika za progresiju bolesti koji bi se koristio u fazi aktivnog praćenja bolesnika
sa ranodijagnostikovanim KP. U poslednje vreme, istraživanja su usmerena na egzozome i njihov ma-
kromolekulski sadržaj (proteine i nekodirajuće molekule RNK) kao potencijalne biološke markere
tečne biopsije KP. Pored toga, biološki makromolekuli na površini egzozoma predstavljaju i ciljane
molekule u novim strategijama lečenja KP., This review-type paper will present the ten-year experience of the PROSTATSERBIA research team,
which has the molecular basis of prostate cancer (PCa) as its research topic. The Center for Human Mo-
lecular Genetics of the Faculty of Biology, University of Belgrade has a collection of samples and a
data bank for almost 1000 men with prostate diseases (PCa and benign prostatic hyperplasia) and for
over 350 men without signs of prostate diseases, all from Serbian population. Most of the studies
were designed as studies of association of selected genetic variants with the risk for development
and progression of PCa. In these case-control studies, genetic variants were examined both in genes
for proteins and non-coding RNAs, as well as in non-coding regions of the genome (so-called gene
deserts). In addition to case control studies, both meta-analyses and the analysis of statistical epista-
tic interactions of the analyzed genetic variants were conducted. The main goal of all these studies was
to create an algorithm for risk assessment for disease progression that would be used in the phase of
active monitoring of patients with early diagnosed PCa. Recently, we shifted our research focus on
exosomes and their macromolecular content (proteins and microRNAs) as potential biological mar-
kers of liquid prostate cancer biopsy. In addition, macromolecules at the exosome surface represent
target molecules for new PCa treatment strategies.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in molecular biology",
booktitle = "Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate, Modern approaches in research of the molecular basis of prostate cancer",
number = "2",
pages = "63-74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6565"
}
Dobrijević, Z., Matijašević-Joković, S., Branković, A., Đorđević, A., Popović, M.,& Brajušković, G.. (2022). Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate. in Trends in molecular biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(2), 63-74.
https://hdl.handle.net/21.15107/rcub_ibiss_6565
Dobrijević Z, Matijašević-Joković S, Branković A, Đorđević A, Popović M, Brajušković G. Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate. in Trends in molecular biology. 2022;(2):63-74.
https://hdl.handle.net/21.15107/rcub_ibiss_6565 .
Dobrijević, Zorana, Matijašević-Joković, Suzana, Branković, Ana, Đorđević, Ana, Popović, Milica, Brajušković, Goran, "Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate" in Trends in molecular biology, no. 2 (2022):63-74,
https://hdl.handle.net/21.15107/rcub_ibiss_6565 .

Fruktoza u ishrani: Ima li razloga za zabrinutost?

Brkljačić, Jelena; Veličković, Nataša; Vojnović-Milutinović, Danijela; Kovačević, Sanja; Teofilović, Ana; Bursać, Biljana; Pešić, Vesna; Đorđević, Ana

(Belgrade: Serbian Biological Society, 2022)

TY  - CONF
AU  - Brkljačić, Jelena
AU  - Veličković, Nataša
AU  - Vojnović-Milutinović, Danijela
AU  - Kovačević, Sanja
AU  - Teofilović, Ana
AU  - Bursać, Biljana
AU  - Pešić, Vesna
AU  - Đorđević, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5710
AB  - Фруктоза је прост шећер одувек присутан у људској исхрани. До 20. века људи су
путем воћа и поврћа уносили релативно ниске количине фруктозе, да би током 20.
века, након увођења високофруктозног кукурузног сирупа у прехрамбену
индустрију, дневни унос фруктозе био учетворостручен што је коинцидирало са
растућом преваленцијом метаболичких поремећаја. Ипак, новије студије показују
да преваленција метаболичких поремећаја и даље расте иако је дневни унос шећера
стабилан или се чак смањује, што указује на допринос других фактора, као што су
смањена физичка активност и свакодневна изложеност стресу. Наша истраживања
на животињском моделу пацова који је храњен фруктозом и хронично излаган
комбинацији стресора пружају одговор на питање да ли, и у којим ситуацијама,
фруктоза може да смањује штетне ефекте стреса, а у којим условима стрес
потенцира штетне ефекте фруктозе? Резултати показују да фруктоза, стрес и
њихова комбинација, на ткивно и полно специфичан начин утичу на метаболизам
глукозе и липида, као и на редокс и инфламаторни статус у јетри, скелетним
мишићима и висцералном масном ткиву, а да поред метаболичких ефеката
фруктоза и стрес утичу и на понашање животиња. Будући да ефекти фруктозе
зависе од дозе и патофизиолошког стања организма, енергија која од ње потиче се
може ефикасно складиштити и по потреби користити кроз физичку активност, док
сталан повећан унос фруктозе, уз седентарни начин живота и стрес може
допринети развоју метаболичких и кардиоваскуларних обољења.
AB  - Fruktoza je prost šećer oduvek prisutan u ljudskoj ishrani. Do 20. veka ljudi su putem voća i povrća unosili relativno niske količine fruktoze, da bi tokom 20. veka, nakon uvođenja visokofruktoznog kukuruznog sirupa u prehrambenu industriju, dnevni unos fruktoze bio učetvorostručen što je koincidiralo sa rastućom prevalencijom metaboličkih poremećaja. Ipak, novije studije pokazuju da prevalencija metaboličkih poremećaja i dalje raste iako je dnevni unos šećera stabilan ili se čak smanjuje, što ukazuje na doprinos drugih faktora, kao što su smanjena fizička aktivnost i svakodnevna izloženost stresu. Naša istraživanja na životinjskom modelu pacova koji je hranjen fruktozom i hronično izlagan kombinaciji stresora pružaju odgovor na pitanje da li, i u kojim situacijama, fruktoza može da smanjuje štetne efekte stresa, a u kojim uslovima stres potencira štetne efekte fruktoze? Rezultati pokazuju da fruktoza, stres i njihova kombinacija, na tkivno i polno specifičan način utiču na metabolizam glukoze i lipida, kao i na redoks i inflamatorni status u jetri, skeletnim mišićima i visceralnom masnom tkivu, a da pored metaboličkih efekata fruktoza i stres utiču i na ponašanje životinja. Budući da efekti fruktoze zavise od doze i patofiziološkog stanja organizma, energija koja od nje potiče se može efikasno skladištiti i po potrebi koristiti kroz fizičku aktivnost, dok stalan povećan unos fruktoze, uz sedentarni način života i stres može doprineti razvoju metaboličkih i kardiovaskularnih oboljenja.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Fruktoza u ishrani: Ima li razloga za zabrinutost?
T1  - Фруктоза у исхрани: Има ли разлога за забринутост?
SP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5710
ER  - 
@conference{
author = "Brkljačić, Jelena and Veličković, Nataša and Vojnović-Milutinović, Danijela and Kovačević, Sanja and Teofilović, Ana and Bursać, Biljana and Pešić, Vesna and Đorđević, Ana",
year = "2022",
abstract = "Фруктоза је прост шећер одувек присутан у људској исхрани. До 20. века људи су
путем воћа и поврћа уносили релативно ниске количине фруктозе, да би током 20.
века, након увођења високофруктозног кукурузног сирупа у прехрамбену
индустрију, дневни унос фруктозе био учетворостручен што је коинцидирало са
растућом преваленцијом метаболичких поремећаја. Ипак, новије студије показују
да преваленција метаболичких поремећаја и даље расте иако је дневни унос шећера
стабилан или се чак смањује, што указује на допринос других фактора, као што су
смањена физичка активност и свакодневна изложеност стресу. Наша истраживања
на животињском моделу пацова који је храњен фруктозом и хронично излаган
комбинацији стресора пружају одговор на питање да ли, и у којим ситуацијама,
фруктоза може да смањује штетне ефекте стреса, а у којим условима стрес
потенцира штетне ефекте фруктозе? Резултати показују да фруктоза, стрес и
њихова комбинација, на ткивно и полно специфичан начин утичу на метаболизам
глукозе и липида, као и на редокс и инфламаторни статус у јетри, скелетним
мишићима и висцералном масном ткиву, а да поред метаболичких ефеката
фруктоза и стрес утичу и на понашање животиња. Будући да ефекти фруктозе
зависе од дозе и патофизиолошког стања организма, енергија која од ње потиче се
може ефикасно складиштити и по потреби користити кроз физичку активност, док
сталан повећан унос фруктозе, уз седентарни начин живота и стрес може
допринети развоју метаболичких и кардиоваскуларних обољења., Fruktoza je prost šećer oduvek prisutan u ljudskoj ishrani. Do 20. veka ljudi su putem voća i povrća unosili relativno niske količine fruktoze, da bi tokom 20. veka, nakon uvođenja visokofruktoznog kukuruznog sirupa u prehrambenu industriju, dnevni unos fruktoze bio učetvorostručen što je koincidiralo sa rastućom prevalencijom metaboličkih poremećaja. Ipak, novije studije pokazuju da prevalencija metaboličkih poremećaja i dalje raste iako je dnevni unos šećera stabilan ili se čak smanjuje, što ukazuje na doprinos drugih faktora, kao što su smanjena fizička aktivnost i svakodnevna izloženost stresu. Naša istraživanja na životinjskom modelu pacova koji je hranjen fruktozom i hronično izlagan kombinaciji stresora pružaju odgovor na pitanje da li, i u kojim situacijama, fruktoza može da smanjuje štetne efekte stresa, a u kojim uslovima stres potencira štetne efekte fruktoze? Rezultati pokazuju da fruktoza, stres i njihova kombinacija, na tkivno i polno specifičan način utiču na metabolizam glukoze i lipida, kao i na redoks i inflamatorni status u jetri, skeletnim mišićima i visceralnom masnom tkivu, a da pored metaboličkih efekata fruktoza i stres utiču i na ponašanje životinja. Budući da efekti fruktoze zavise od doze i patofiziološkog stanja organizma, energija koja od nje potiče se može efikasno skladištiti i po potrebi koristiti kroz fizičku aktivnost, dok stalan povećan unos fruktoze, uz sedentarni način života i stres može doprineti razvoju metaboličkih i kardiovaskularnih oboljenja.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Fruktoza u ishrani: Ima li razloga za zabrinutost?, Фруктоза у исхрани: Има ли разлога за забринутост?",
pages = "284",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5710"
}
Brkljačić, J., Veličković, N., Vojnović-Milutinović, D., Kovačević, S., Teofilović, A., Bursać, B., Pešić, V.,& Đorđević, A.. (2022). Fruktoza u ishrani: Ima li razloga za zabrinutost?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 284.
https://hdl.handle.net/21.15107/rcub_ibiss_5710
Brkljačić J, Veličković N, Vojnović-Milutinović D, Kovačević S, Teofilović A, Bursać B, Pešić V, Đorđević A. Fruktoza u ishrani: Ima li razloga za zabrinutost?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:284.
https://hdl.handle.net/21.15107/rcub_ibiss_5710 .
Brkljačić, Jelena, Veličković, Nataša, Vojnović-Milutinović, Danijela, Kovačević, Sanja, Teofilović, Ana, Bursać, Biljana, Pešić, Vesna, Đorđević, Ana, "Fruktoza u ishrani: Ima li razloga za zabrinutost?" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):284,
https://hdl.handle.net/21.15107/rcub_ibiss_5710 .

Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.

Kolarević, Stoimir; Micsinai, Adrienn; Szántó-Egész, Réka; Lukács, Alena; Kračun-Kolarević, Margareta; Đorđević, Ana; Vojnović-Milutinović, Danijela; Jovanović Marić, Jovana; Kirschner, Alexander K T; Farnleitner, Andreas A H; Linke, Rita; Đukić, Aleksandar; Kostić-Vuković, Jovana; Paunović, Momir

(Amsterdam: Elsevier, 2022)

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Jovanović Marić, Jovana
AU  - Kirschner, Alexander K T
AU  - Farnleitner, Andreas A H
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S004896972204061X
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9232394
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5072
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Amsterdam: Elsevier
T2  - Science of The Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
SP  - 156964
ER  - 
@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Vojnović-Milutinović, Danijela and Jovanović Marić, Jovana and Kirschner, Alexander K T and Farnleitner, Andreas A H and Linke, Rita and Đukić, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Amsterdam: Elsevier",
journal = "Science of The Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964",
pages = "156964"
}
Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Vojnović-Milutinović, D., Jovanović Marić, J., Kirschner, A. K. T., Farnleitner, A. A. H., Linke, R., Đukić, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.. in Science of The Total Environment
Amsterdam: Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964
Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Vojnović-Milutinović D, Jovanović Marić J, Kirschner AKT, Farnleitner AAH, Linke R, Đukić A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.. in Science of The Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .
Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Vojnović-Milutinović, Danijela, Jovanović Marić, Jovana, Kirschner, Alexander K T, Farnleitner, Andreas A H, Linke, Rita, Đukić, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters." in Science of The Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .
5
4
3

Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function

Tasić, Danica; Opačić, Miloš; Kovačević, Sanja; Nikolić-Kokić, Aleksandra; Dimitrijević, Milena; Nikolić, Dušan; Vojnović-Milutinović, Danijela; Blagojević, Duško; Đorđević, Ana; Brkljačić, Jelena

(Basel: MDPI, 2022)

TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović-Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5025
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys
are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression
of metabolic disturbances in the kidney. We determined protein level of copper transporters,
chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes
function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation
and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of
superoxide dismutase and decreased metallothionein level, while rendering the level of copper
importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi
network unaffected. Stress had no effect on renal copper metabolism. The activity and expression
of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose,
independently of stress, decreased renal copper level, and modulated renal copper metabolism
as to preserve vital cellular function including mitochondrial energy production and antioxidative
defense, at the expense of intracellular copper storage.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
VL  - 23
DO  - 10.3390/ijms23169023
SP  - 9023
ER  - 
@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović-Milutinović, Danijela and Blagojević, Duško and Đorđević, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys
are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression
of metabolic disturbances in the kidney. We determined protein level of copper transporters,
chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes
function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation
and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of
superoxide dismutase and decreased metallothionein level, while rendering the level of copper
importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi
network unaffected. Stress had no effect on renal copper metabolism. The activity and expression
of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose,
independently of stress, decreased renal copper level, and modulated renal copper metabolism
as to preserve vital cellular function including mitochondrial energy production and antioxidative
defense, at the expense of intracellular copper storage.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
volume = "23",
doi = "10.3390/ijms23169023",
pages = "9023"
}
Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović-Milutinović, D., Blagojević, D., Đorđević, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
Basel: MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023
Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović-Milutinović D, Blagojević D, Đorđević A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .
Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović-Milutinović, Danijela, Blagojević, Duško, Đorđević, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .
3
3

Combination of chronic stress with fructose diet increases AMPactivated protein kinase phosphorylation and affects agoutirelated protein and proopiomelanocortin expression in the hypothalamus of male Wistar rats

Sirif, Zidane Abdulbaset; Kovačević, Sanja; Bursać, Biljana; Lakić, Iva; Veličković, Nataša; Jevđović, Tanja; Đorđević, Ana

(Warsaw: Polish Biochemical Society, 2022)

TY  - JOUR
AU  - Sirif, Zidane Abdulbaset
AU  - Kovačević, Sanja
AU  - Bursać, Biljana
AU  - Lakić, Iva
AU  - Veličković, Nataša
AU  - Jevđović, Tanja
AU  - Đorđević, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5024
AB  - Appetite regulation in the hypothalamus is dependent on
hormonal signals from the periphery, such as insulin and
leptin, and can be modulated by both sugar-rich diet and
stress. Our aim was to explore the effects of 9-week feeding
with 20% fructose solution combined with 4-week
chronic unpredictable stress, on appetite-regulating neuropeptides
and modulatory role of leptin and insulin signalling
in the hypothalamus of male Wistar rats. Energy
intake, body mass and adiposity, as well as circulatory
leptin and insulin concentrations were assessed. Hypothalamic
insulin signalling was analysed at the level of glucose
transporters, as well as at the protein level and phosphorylation
of insulin receptor, insulin receptor supstrate-1, Akt
and ERK. Phosphorylation of AMP-activated protein kinase
(AMPK), level of protein tyrosine phosphatase 1B (PTP1B)
and expression of leptin receptor (ObRb) and suppressor of
cytokine signalling 3 (SOCS3) were also analysed, together
with the expression of orexigenic agouti-related protein
(AgRP) and anorexigenic proopiomelanocortin (POMC) neuropeptides.
The results revealed that stress decreased body
mass and adiposity, blood leptin level and expression of
ObRb, SOCS3 and POMC, while combination with fructose
diet led to marked increase of AgRP, associated with AMPK
phosphorylation despite increased plasma insulin. Reduced
Akt, enhanced ERK activity and elevated PTP1B were also
observed in the hypothalamus of these animals. In conclusion,
our results showed that joint effects of fructose diet
and stress are more deleterious than the separate ones,
since inappropriate appetite control in the hypothalamus
may provide a setting for the disturbed energy homeostasis
in the long run.
PB  - Warsaw: Polish Biochemical Society
T2  - Acta Biochimica Polonica
T1  - Combination of chronic stress with fructose diet increases AMPactivated protein kinase phosphorylation and affects agoutirelated protein and proopiomelanocortin expression in the hypothalamus of male Wistar rats
IS  - 6075
DO  - 10.18388/abp.2020_6075
ER  - 
@article{
author = "Sirif, Zidane Abdulbaset and Kovačević, Sanja and Bursać, Biljana and Lakić, Iva and Veličković, Nataša and Jevđović, Tanja and Đorđević, Ana",
year = "2022",
abstract = "Appetite regulation in the hypothalamus is dependent on
hormonal signals from the periphery, such as insulin and
leptin, and can be modulated by both sugar-rich diet and
stress. Our aim was to explore the effects of 9-week feeding
with 20% fructose solution combined with 4-week
chronic unpredictable stress, on appetite-regulating neuropeptides
and modulatory role of leptin and insulin signalling
in the hypothalamus of male Wistar rats. Energy
intake, body mass and adiposity, as well as circulatory
leptin and insulin concentrations were assessed. Hypothalamic
insulin signalling was analysed at the level of glucose
transporters, as well as at the protein level and phosphorylation
of insulin receptor, insulin receptor supstrate-1, Akt
and ERK. Phosphorylation of AMP-activated protein kinase
(AMPK), level of protein tyrosine phosphatase 1B (PTP1B)
and expression of leptin receptor (ObRb) and suppressor of
cytokine signalling 3 (SOCS3) were also analysed, together
with the expression of orexigenic agouti-related protein
(AgRP) and anorexigenic proopiomelanocortin (POMC) neuropeptides.
The results revealed that stress decreased body
mass and adiposity, blood leptin level and expression of
ObRb, SOCS3 and POMC, while combination with fructose
diet led to marked increase of AgRP, associated with AMPK
phosphorylation despite increased plasma insulin. Reduced
Akt, enhanced ERK activity and elevated PTP1B were also
observed in the hypothalamus of these animals. In conclusion,
our results showed that joint effects of fructose diet
and stress are more deleterious than the separate ones,
since inappropriate appetite control in the hypothalamus
may provide a setting for the disturbed energy homeostasis
in the long run.",
publisher = "Warsaw: Polish Biochemical Society",
journal = "Acta Biochimica Polonica",
title = "Combination of chronic stress with fructose diet increases AMPactivated protein kinase phosphorylation and affects agoutirelated protein and proopiomelanocortin expression in the hypothalamus of male Wistar rats",
number = "6075",
doi = "10.18388/abp.2020_6075"
}
Sirif, Z. A., Kovačević, S., Bursać, B., Lakić, I., Veličković, N., Jevđović, T.,& Đorđević, A.. (2022). Combination of chronic stress with fructose diet increases AMPactivated protein kinase phosphorylation and affects agoutirelated protein and proopiomelanocortin expression in the hypothalamus of male Wistar rats. in Acta Biochimica Polonica
Warsaw: Polish Biochemical Society.(6075).
https://doi.org/10.18388/abp.2020_6075
Sirif ZA, Kovačević S, Bursać B, Lakić I, Veličković N, Jevđović T, Đorđević A. Combination of chronic stress with fructose diet increases AMPactivated protein kinase phosphorylation and affects agoutirelated protein and proopiomelanocortin expression in the hypothalamus of male Wistar rats. in Acta Biochimica Polonica. 2022;(6075).
doi:10.18388/abp.2020_6075 .
Sirif, Zidane Abdulbaset, Kovačević, Sanja, Bursać, Biljana, Lakić, Iva, Veličković, Nataša, Jevđović, Tanja, Đorđević, Ana, "Combination of chronic stress with fructose diet increases AMPactivated protein kinase phosphorylation and affects agoutirelated protein and proopiomelanocortin expression in the hypothalamus of male Wistar rats" in Acta Biochimica Polonica, no. 6075 (2022),
https://doi.org/10.18388/abp.2020_6075 . .
1
1

Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle

Prodanović, Radiša; Kirovski, Danijela; Vujanac, Ivan; Đorđević, Ana; Romić, Snježana; Pantelić, Marija; Korićanac, Goran

(Amsterdam: Elsevier Ltd., 2022)

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Romić, Snježana
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5023
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.
PB  - Amsterdam: Elsevier Ltd.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
DO  - 10.1016/j.rvsc.2022.04.012
SP  - 189
EP  - 194
ER  - 
@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana and Romić, Snježana and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.",
publisher = "Amsterdam: Elsevier Ltd.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
doi = "10.1016/j.rvsc.2022.04.012",
pages = "189-194"
}
Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A., Romić, S., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science
Amsterdam: Elsevier Ltd.., 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012
Prodanović R, Kirovski D, Vujanac I, Đorđević A, Romić S, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .
Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana, Romić, Snježana, Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .
1
1

AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome

Mićić, Bojana; Teofilović, Ana; Đorđević, Ana; Veličković, Nataša; Macut, Djuro; Vojnović-Milutinović, Danijela

(Basel: MDPI, 2022)

TY  - JOUR
AU  - Mićić, Bojana
AU  - Teofilović, Ana
AU  - Đorđević, Ana
AU  - Veličković, Nataša
AU  - Macut, Djuro
AU  - Vojnović-Milutinović, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5022
AB  - Polycystic ovary syndrome (PCOS) is a well-known reproductive syndrome usually associated
with obesity, insulin resistance, and hyperinsulinemia. Although the first signs of PCOS
begin early in adolescence, it is underexplored whether peripubertal obesity predisposes women to
PCOS metabolic disturbances. To highlight that, we examined the impact of postnatal overfeedinginduced
obesity, achieved by litter size reduction during the suckling period, on metabolic disturbances
associated with visceral and subcutaneous adipose tissue (VAT and SAT) function in the
5 -dihydrotestosterone (5 -DHT)-induced animal model of PCOS. We analyzed markers of insulin
signaling, lipid metabolism, and energy sensing in the VAT and SAT. Our results showed that postnatally
overfed DHT-treated Wistar rats had increased VAT mass with hypertrophic adipocytes,
together with hyperinsulinemia and increased HOMA index. In the VAT of these animals, insulin
signaling remained unchanged while lipogenic markers decreased, which was accompanied by
increased AMPK activation. In the SAT of the same animals, markers of lipogenesis and lipolysis
increased, while the activity of AMPK decreased. Taken together, obtained results showed that
postnatal overfeeding predisposes development of PCOS systemic insulin resistance, most likely as
a result of worsened metabolic function of SAT, while VAT preserved its tissue insulin sensitivity
through increased activity of AMPK.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome
IS  - 16
VL  - 23
DO  - 10.3390/ijms23168942
SP  - 8942
ER  - 
@article{
author = "Mićić, Bojana and Teofilović, Ana and Đorđević, Ana and Veličković, Nataša and Macut, Djuro and Vojnović-Milutinović, Danijela",
year = "2022",
abstract = "Polycystic ovary syndrome (PCOS) is a well-known reproductive syndrome usually associated
with obesity, insulin resistance, and hyperinsulinemia. Although the first signs of PCOS
begin early in adolescence, it is underexplored whether peripubertal obesity predisposes women to
PCOS metabolic disturbances. To highlight that, we examined the impact of postnatal overfeedinginduced
obesity, achieved by litter size reduction during the suckling period, on metabolic disturbances
associated with visceral and subcutaneous adipose tissue (VAT and SAT) function in the
5 -dihydrotestosterone (5 -DHT)-induced animal model of PCOS. We analyzed markers of insulin
signaling, lipid metabolism, and energy sensing in the VAT and SAT. Our results showed that postnatally
overfed DHT-treated Wistar rats had increased VAT mass with hypertrophic adipocytes,
together with hyperinsulinemia and increased HOMA index. In the VAT of these animals, insulin
signaling remained unchanged while lipogenic markers decreased, which was accompanied by
increased AMPK activation. In the SAT of the same animals, markers of lipogenesis and lipolysis
increased, while the activity of AMPK decreased. Taken together, obtained results showed that
postnatal overfeeding predisposes development of PCOS systemic insulin resistance, most likely as
a result of worsened metabolic function of SAT, while VAT preserved its tissue insulin sensitivity
through increased activity of AMPK.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome",
number = "16",
volume = "23",
doi = "10.3390/ijms23168942",
pages = "8942"
}
Mićić, B., Teofilović, A., Đorđević, A., Veličković, N., Macut, D.,& Vojnović-Milutinović, D.. (2022). AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome. in International Journal of Molecular Sciences
Basel: MDPI., 23(16), 8942.
https://doi.org/10.3390/ijms23168942
Mićić B, Teofilović A, Đorđević A, Veličković N, Macut D, Vojnović-Milutinović D. AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome. in International Journal of Molecular Sciences. 2022;23(16):8942.
doi:10.3390/ijms23168942 .
Mićić, Bojana, Teofilović, Ana, Đorđević, Ana, Veličković, Nataša, Macut, Djuro, Vojnović-Milutinović, Danijela, "AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome" in International Journal of Molecular Sciences, 23, no. 16 (2022):8942,
https://doi.org/10.3390/ijms23168942 . .
3
3

Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats.

Teofilović, Ana; Vratarić, Miloš; Veličković, Nataša; Vojnović-Milutinović, Danijela; Mladenović, Aleksandra; Prvulović, Milica; Đorđević, Ana

(2022)

TY  - JOUR
AU  - Teofilović, Ana
AU  - Vratarić, Miloš
AU  - Veličković, Nataša
AU  - Vojnović-Milutinović, Danijela
AU  - Mladenović, Aleksandra
AU  - Prvulović, Milica
AU  - Đorđević, Ana
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.899255/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9168317
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5004
AB  - Aging is a progressive process that could disturb metabolic homeostasis in the liver via ectopic lipid accumulation, oxidative stress, and deterioration of inflammatory response. Although calorie restriction (CR) is recognized as beneficial for life span and health span prolongation, it is still unclear how late-onset CR, characterized by late beginning and short duration, affects age-related processes. The aim of this study was to examine how late-onset CR-induced metabolic adjustments impact lipid status and inflammation in the liver of old rats. The experiments were conducted on aging male Wistar rats fed ad libitum (AL) or exposed to late-onset CR (60% of AL daily intake) from 21st to 24th month. The results showed that late-onset CR reduces body weight, visceral adipose tissue and liver mass, and triglyceride levels when compared to old animals on AL diet. The ameliorating effects of CR on lipid metabolism include increased activity of AMP-activated protein kinase, suppressed de novo fatty acid synthesis, stimulated β-oxidation, decreased lipotoxicity, and limited triglyceride synthesis and packaging in the liver. Restricted diet regime, however, does not improve expression of antioxidant enzymes, although it leads to progression of age-related inflammation in the liver, partially through lower corticosterone concentration and decreased activation of glucocorticoid receptor. In conclusion, late-onset CR is able to restore age-related imbalance of lipid metabolism in the liver, but has a negative impact on hepatic inflammatory status, implying that the type of diet for older individuals must be balanced and chosen carefully with appropriate duration and start point.
T2  - Frontiers in Nutrition
T1  - Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats.
VL  - 9
DO  - 10.3389/fnut.2022.899255
SP  - 899255
ER  - 
@article{
author = "Teofilović, Ana and Vratarić, Miloš and Veličković, Nataša and Vojnović-Milutinović, Danijela and Mladenović, Aleksandra and Prvulović, Milica and Đorđević, Ana",
year = "2022",
abstract = "Aging is a progressive process that could disturb metabolic homeostasis in the liver via ectopic lipid accumulation, oxidative stress, and deterioration of inflammatory response. Although calorie restriction (CR) is recognized as beneficial for life span and health span prolongation, it is still unclear how late-onset CR, characterized by late beginning and short duration, affects age-related processes. The aim of this study was to examine how late-onset CR-induced metabolic adjustments impact lipid status and inflammation in the liver of old rats. The experiments were conducted on aging male Wistar rats fed ad libitum (AL) or exposed to late-onset CR (60% of AL daily intake) from 21st to 24th month. The results showed that late-onset CR reduces body weight, visceral adipose tissue and liver mass, and triglyceride levels when compared to old animals on AL diet. The ameliorating effects of CR on lipid metabolism include increased activity of AMP-activated protein kinase, suppressed de novo fatty acid synthesis, stimulated β-oxidation, decreased lipotoxicity, and limited triglyceride synthesis and packaging in the liver. Restricted diet regime, however, does not improve expression of antioxidant enzymes, although it leads to progression of age-related inflammation in the liver, partially through lower corticosterone concentration and decreased activation of glucocorticoid receptor. In conclusion, late-onset CR is able to restore age-related imbalance of lipid metabolism in the liver, but has a negative impact on hepatic inflammatory status, implying that the type of diet for older individuals must be balanced and chosen carefully with appropriate duration and start point.",
journal = "Frontiers in Nutrition",
title = "Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats.",
volume = "9",
doi = "10.3389/fnut.2022.899255",
pages = "899255"
}
Teofilović, A., Vratarić, M., Veličković, N., Vojnović-Milutinović, D., Mladenović, A., Prvulović, M.,& Đorđević, A.. (2022). Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats.. in Frontiers in Nutrition, 9, 899255.
https://doi.org/10.3389/fnut.2022.899255
Teofilović A, Vratarić M, Veličković N, Vojnović-Milutinović D, Mladenović A, Prvulović M, Đorđević A. Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats.. in Frontiers in Nutrition. 2022;9:899255.
doi:10.3389/fnut.2022.899255 .
Teofilović, Ana, Vratarić, Miloš, Veličković, Nataša, Vojnović-Milutinović, Danijela, Mladenović, Aleksandra, Prvulović, Milica, Đorđević, Ana, "Late-Onset Calorie Restriction Improves Lipid Metabolism and Aggravates Inflammation in the Liver of Old Wistar Rats." in Frontiers in Nutrition, 9 (2022):899255,
https://doi.org/10.3389/fnut.2022.899255 . .
2
8

Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells

Mićić, Bojana; Radovanović, Marina; Tovilović-Kovačević, Gordana; Teofilović, Ana; Gligorovska, Ljupka; Vojnović-Milutinović, Danijela; Đorđević, Ana; Ignjatović, Đurđica

(Elsevier Inc., 2021)

TY  - CONF
AU  - Mićić, Bojana
AU  - Radovanović, Marina
AU  - Tovilović-Kovačević, Gordana
AU  - Teofilović, Ana
AU  - Gligorovska, Ljupka
AU  - Vojnović-Milutinović, Danijela
AU  - Đorđević, Ana
AU  - Ignjatović, Đurđica
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4718
AB  - Fructose intake is associated with low-grade inflammation and increased oxidative
stress. Among long chain polyunsaturated fatty acids (LC-PUFAs), Ω-6 are recognized as a
contributing factor to inflammation, while Ω-3 LC-PUFAs are considered as functional foods with
beneficial effects, including inhibition of pro-inflammatory pathways. The aim of this study was to
analyze combined effects of physiologically relevant LC-PUFAs and fructose on inflammation and
antioxidant enzymes in the in vitro model of vascular endothelial cells. We examined the effects
of 0.5 mM fructose, alone and in combination with Ω-6 (arachidonic (AA) and linoleic (LA)) and
Ω-3 (eicosapentaenoic (EPA) and docosahexaenoic acid (DHA)) LC-PUFAs on expression of pro-
inflammatory cytokines (tumor necrosis factor α (TNFα) and interleukin 6 (IL6)) in EA.hy926 cells.
The protein levels of nuclear factor-κB (NF-κB) and IB, as well as its phosphorylation, together
with superoxide dismutase (SOD) 1 and 2, catalase and glutathione reductase (GR) were also
analyzed. Total ROS amounts were determined using flow cytometric analysis of cells stained
with redox sensitive dihydrorhodamine 123 dye. The results showed that treatment of cells with
fructose increased TNFα and decreased IL6 mRNA levels. Additional treatment with LA, DHA
and EPA reduced TNFα and led to further decrease of IL6 expression. The observed changes
were not associated with NFB activation. All examined enzymes were unchanged after fructose
treatment, while GR was increased by LC-PUFA addition. SOD2 was reduced in cells treated
with AA, LA and EPA, while increased ROS amounts were observed with AA, DHA and EPA. This
was also evident in combined treatment with fructose. These preliminary results suggest that
LC-PUFAs, besides effect on pro-inflammatory cytokines, reduce SOD2 levels and increase ROS.
The increased levels of ROS could stimulate production of PUFA-derived peroxides, which in
GSH-enriched environment might be converted into anti-inflammatory derivatives, additionally
suppressing inflammation in fructose treated endothelial cells
PB  - Elsevier Inc.
C3  - Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
T1  - Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells
DO  - 10.1016/j.freeradbiomed.2021.08.092
SP  - S79
ER  - 
@conference{
author = "Mićić, Bojana and Radovanović, Marina and Tovilović-Kovačević, Gordana and Teofilović, Ana and Gligorovska, Ljupka and Vojnović-Milutinović, Danijela and Đorđević, Ana and Ignjatović, Đurđica",
year = "2021",
abstract = "Fructose intake is associated with low-grade inflammation and increased oxidative
stress. Among long chain polyunsaturated fatty acids (LC-PUFAs), Ω-6 are recognized as a
contributing factor to inflammation, while Ω-3 LC-PUFAs are considered as functional foods with
beneficial effects, including inhibition of pro-inflammatory pathways. The aim of this study was to
analyze combined effects of physiologically relevant LC-PUFAs and fructose on inflammation and
antioxidant enzymes in the in vitro model of vascular endothelial cells. We examined the effects
of 0.5 mM fructose, alone and in combination with Ω-6 (arachidonic (AA) and linoleic (LA)) and
Ω-3 (eicosapentaenoic (EPA) and docosahexaenoic acid (DHA)) LC-PUFAs on expression of pro-
inflammatory cytokines (tumor necrosis factor α (TNFα) and interleukin 6 (IL6)) in EA.hy926 cells.
The protein levels of nuclear factor-κB (NF-κB) and IB, as well as its phosphorylation, together
with superoxide dismutase (SOD) 1 and 2, catalase and glutathione reductase (GR) were also
analyzed. Total ROS amounts were determined using flow cytometric analysis of cells stained
with redox sensitive dihydrorhodamine 123 dye. The results showed that treatment of cells with
fructose increased TNFα and decreased IL6 mRNA levels. Additional treatment with LA, DHA
and EPA reduced TNFα and led to further decrease of IL6 expression. The observed changes
were not associated with NFB activation. All examined enzymes were unchanged after fructose
treatment, while GR was increased by LC-PUFA addition. SOD2 was reduced in cells treated
with AA, LA and EPA, while increased ROS amounts were observed with AA, DHA and EPA. This
was also evident in combined treatment with fructose. These preliminary results suggest that
LC-PUFAs, besides effect on pro-inflammatory cytokines, reduce SOD2 levels and increase ROS.
The increased levels of ROS could stimulate production of PUFA-derived peroxides, which in
GSH-enriched environment might be converted into anti-inflammatory derivatives, additionally
suppressing inflammation in fructose treated endothelial cells",
publisher = "Elsevier Inc.",
journal = "Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia",
title = "Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells",
doi = "10.1016/j.freeradbiomed.2021.08.092",
pages = "S79"
}
Mićić, B., Radovanović, M., Tovilović-Kovačević, G., Teofilović, A., Gligorovska, L., Vojnović-Milutinović, D., Đorđević, A.,& Ignjatović, Đ.. (2021). Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
Elsevier Inc.., S79.
https://doi.org/10.1016/j.freeradbiomed.2021.08.092
Mićić B, Radovanović M, Tovilović-Kovačević G, Teofilović A, Gligorovska L, Vojnović-Milutinović D, Đorđević A, Ignjatović Đ. Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia. 2021;:S79.
doi:10.1016/j.freeradbiomed.2021.08.092 .
Mićić, Bojana, Radovanović, Marina, Tovilović-Kovačević, Gordana, Teofilović, Ana, Gligorovska, Ljupka, Vojnović-Milutinović, Danijela, Đorđević, Ana, Ignjatović, Đurđica, "Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells" in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia (2021):S79,
https://doi.org/10.1016/j.freeradbiomed.2021.08.092 . .

Glucocorticoid signaling and lipid metabolism disturbances in the liver of rats treated with 5α-dihydrotestosterone in an animal model of polycystic ovary syndrome.

Vojnović-Milutinović, Danijela; Teofilović, Ana; Veličković, Nataša; Brkljačić, Jelena; Jelača, Sanja; Đorđević, Ana; Macut, Đuro

(Springer, 2021)

TY  - JOUR
AU  - Vojnović-Milutinović, Danijela
AU  - Teofilović, Ana
AU  - Veličković, Nataša
AU  - Brkljačić, Jelena
AU  - Jelača, Sanja
AU  - Đorđević, Ana
AU  - Macut, Đuro
PY  - 2021
UR  - https://doi.org/10.1007/s12020-020-02600-1
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33449293
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4146
AB  - PURPOSE Polycystic ovary syndrome (PCOS) is a complex reproductive disorder often associated with obesity, insulin resistance, and dyslipidemia. Hormonal changes in PCOS may also include altered glucocorticoid signaling. Our aim was to examine whether alterations in hepatic glucocorticoid signaling are associated with disturbances of glucose and lipid metabolism in animal model of PCOS. METHODS Female rats, 3 weeks old, were subcutaneously implanted with 5α-dihydrotestosterone (DHT) or placebo pellets for 90 days to induce PCOS. Expression of 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) and A-ring reductases (5α and 5β), as well as intracellular distribution of glucocorticoid receptor (GR) and expression of its regulated genes were examined in the liver. Proteins of hepatic lipid and carbohydrate metabolism and markers of inflammation were also assessed. RESULTS DHT treatment induced increase in body and liver mass, as well as in triglycerides and free fatty acids levels in plasma. Elevation of 11βHSD1 and reduction of 5α-reductase expression was observed together with increased hepatic corticosterone concentration and nuclear GR activation. Induced expression of Krüppel-like factor 15 and decreased expression of genes for proinflammatory cytokines and de novo lipogenesis (DNL) were detected in the liver of DHT-treated rats, while DNL regulators and proinflammatory markers were not changed. However, increased mRNA levels of stearoyl-CoA desaturase and apolipoprotein B were observed in DHT animals. CONCLUSIONS DHT treatment stimulated hepatic glucocorticoid prereceptor metabolism through increased corticosterone availability which is associated with enhanced GR activation. This does not affect gluconeogenesis and DNL, but could be linked to stimulated triglyceride synthesis and hypertriglyceridemia.
PB  - Springer
T2  - Endocrine
T1  - Glucocorticoid signaling and lipid metabolism disturbances in the liver of rats treated with 5α-dihydrotestosterone in an animal model of polycystic ovary syndrome.
DO  - 10.1007/s12020-020-02600-1
ER  - 
@article{
author = "Vojnović-Milutinović, Danijela and Teofilović, Ana and Veličković, Nataša and Brkljačić, Jelena and Jelača, Sanja and Đorđević, Ana and Macut, Đuro",
year = "2021",
abstract = "PURPOSE Polycystic ovary syndrome (PCOS) is a complex reproductive disorder often associated with obesity, insulin resistance, and dyslipidemia. Hormonal changes in PCOS may also include altered glucocorticoid signaling. Our aim was to examine whether alterations in hepatic glucocorticoid signaling are associated with disturbances of glucose and lipid metabolism in animal model of PCOS. METHODS Female rats, 3 weeks old, were subcutaneously implanted with 5α-dihydrotestosterone (DHT) or placebo pellets for 90 days to induce PCOS. Expression of 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) and A-ring reductases (5α and 5β), as well as intracellular distribution of glucocorticoid receptor (GR) and expression of its regulated genes were examined in the liver. Proteins of hepatic lipid and carbohydrate metabolism and markers of inflammation were also assessed. RESULTS DHT treatment induced increase in body and liver mass, as well as in triglycerides and free fatty acids levels in plasma. Elevation of 11βHSD1 and reduction of 5α-reductase expression was observed together with increased hepatic corticosterone concentration and nuclear GR activation. Induced expression of Krüppel-like factor 15 and decreased expression of genes for proinflammatory cytokines and de novo lipogenesis (DNL) were detected in the liver of DHT-treated rats, while DNL regulators and proinflammatory markers were not changed. However, increased mRNA levels of stearoyl-CoA desaturase and apolipoprotein B were observed in DHT animals. CONCLUSIONS DHT treatment stimulated hepatic glucocorticoid prereceptor metabolism through increased corticosterone availability which is associated with enhanced GR activation. This does not affect gluconeogenesis and DNL, but could be linked to stimulated triglyceride synthesis and hypertriglyceridemia.",
publisher = "Springer",
journal = "Endocrine",
title = "Glucocorticoid signaling and lipid metabolism disturbances in the liver of rats treated with 5α-dihydrotestosterone in an animal model of polycystic ovary syndrome.",
doi = "10.1007/s12020-020-02600-1"
}
Vojnović-Milutinović, D., Teofilović, A., Veličković, N., Brkljačić, J., Jelača, S., Đorđević, A.,& Macut, Đ.. (2021). Glucocorticoid signaling and lipid metabolism disturbances in the liver of rats treated with 5α-dihydrotestosterone in an animal model of polycystic ovary syndrome.. in Endocrine
Springer..
https://doi.org/10.1007/s12020-020-02600-1
Vojnović-Milutinović D, Teofilović A, Veličković N, Brkljačić J, Jelača S, Đorđević A, Macut Đ. Glucocorticoid signaling and lipid metabolism disturbances in the liver of rats treated with 5α-dihydrotestosterone in an animal model of polycystic ovary syndrome.. in Endocrine. 2021;.
doi:10.1007/s12020-020-02600-1 .
Vojnović-Milutinović, Danijela, Teofilović, Ana, Veličković, Nataša, Brkljačić, Jelena, Jelača, Sanja, Đorđević, Ana, Macut, Đuro, "Glucocorticoid signaling and lipid metabolism disturbances in the liver of rats treated with 5α-dihydrotestosterone in an animal model of polycystic ovary syndrome." in Endocrine (2021),
https://doi.org/10.1007/s12020-020-02600-1 . .
8
1
6

Macrophage migration inhibitory factor deficiency aggravates effects of fructose‐enriched diet on lipid metabolism in the mouse liver

Gligorovska, Ljupka; Teofilović, Ana; Vojnović-Milutinović, Danijela; Miladinović, Nenad; Kovačević, Sanja; Veličković, Nataša; Đorđević, Ana

(Blackwell Publishing Inc., 2021)

TY  - JOUR
AU  - Gligorovska, Ljupka
AU  - Teofilović, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Miladinović, Nenad
AU  - Kovačević, Sanja
AU  - Veličković, Nataša
AU  - Đorđević, Ana
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/10.1002/biof.1711
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4148
AB  - Dietary fructose can disturb hepatic lipid metabolism in a way that leads to lipid accumulation and steatosis, which is often accompanied with low-grade inflammation. The macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine with important role not only in the regulation of inflammation, but also in the modulation of energy metabolism in the liver. Thus, the aim of this study was to investigate the role of Mif deficiency in fructose-induced disturbances of hepatic lipid metabolism and ectopic lipid accumulation. Wild type (WT) and Mif deficient (MIF−/−) C57Bl/6J mice were used to analyze the effects of 9-week 20% fructose-enriched diet on hepatic lipid metabolism (both lipogenesis and β-oxidation) and histology, inflammatory status and glucocorticoid receptor (GR) signaling. The results showed fructose-induced elevation of lipogenic genes (fatty acid synthase (Fas) and stearoyl-CoA desaturase-1 (Scd1) and transcriptional lipogenic regulators (liver X receptor (LXR), sterol regulatory element binding protein 1c (SREBP1c), and carbohydrate response element-binding protein (ChREBP)). However, microvesicular fatty changes, accompanied with enhanced inflammation, were observable only in fructose-fed Mif deficient animals, and were most likely result of GR activation and facilitated uptake and decreased β-oxidation of FFA, as evidenced by elevated protein level of fatty acid translocase (FAT/CD36) and decreased carnitine palmitoyl transferase 1 (CPT1) level. In conclusion, the results show that Mif deficiency aggravates the effects of energy-rich fructose diet on hepatic lipid accumulation, most likely through enhanced inflammation and activation of GR signaling pathway.
PB  - Blackwell Publishing Inc.
T2  - BioFactors
T1  - Macrophage migration inhibitory factor deficiency aggravates effects of fructose‐enriched diet on lipid metabolism in the mouse liver
DO  - 10.1002/biof.1711
SP  - biof.1711
ER  - 
@article{
author = "Gligorovska, Ljupka and Teofilović, Ana and Vojnović-Milutinović, Danijela and Miladinović, Nenad and Kovačević, Sanja and Veličković, Nataša and Đorđević, Ana",
year = "2021",
abstract = "Dietary fructose can disturb hepatic lipid metabolism in a way that leads to lipid accumulation and steatosis, which is often accompanied with low-grade inflammation. The macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine with important role not only in the regulation of inflammation, but also in the modulation of energy metabolism in the liver. Thus, the aim of this study was to investigate the role of Mif deficiency in fructose-induced disturbances of hepatic lipid metabolism and ectopic lipid accumulation. Wild type (WT) and Mif deficient (MIF−/−) C57Bl/6J mice were used to analyze the effects of 9-week 20% fructose-enriched diet on hepatic lipid metabolism (both lipogenesis and β-oxidation) and histology, inflammatory status and glucocorticoid receptor (GR) signaling. The results showed fructose-induced elevation of lipogenic genes (fatty acid synthase (Fas) and stearoyl-CoA desaturase-1 (Scd1) and transcriptional lipogenic regulators (liver X receptor (LXR), sterol regulatory element binding protein 1c (SREBP1c), and carbohydrate response element-binding protein (ChREBP)). However, microvesicular fatty changes, accompanied with enhanced inflammation, were observable only in fructose-fed Mif deficient animals, and were most likely result of GR activation and facilitated uptake and decreased β-oxidation of FFA, as evidenced by elevated protein level of fatty acid translocase (FAT/CD36) and decreased carnitine palmitoyl transferase 1 (CPT1) level. In conclusion, the results show that Mif deficiency aggravates the effects of energy-rich fructose diet on hepatic lipid accumulation, most likely through enhanced inflammation and activation of GR signaling pathway.",
publisher = "Blackwell Publishing Inc.",
journal = "BioFactors",
title = "Macrophage migration inhibitory factor deficiency aggravates effects of fructose‐enriched diet on lipid metabolism in the mouse liver",
doi = "10.1002/biof.1711",
pages = "biof.1711"
}
Gligorovska, L., Teofilović, A., Vojnović-Milutinović, D., Miladinović, N., Kovačević, S., Veličković, N.,& Đorđević, A.. (2021). Macrophage migration inhibitory factor deficiency aggravates effects of fructose‐enriched diet on lipid metabolism in the mouse liver. in BioFactors
Blackwell Publishing Inc.., biof.1711.
https://doi.org/10.1002/biof.1711
Gligorovska L, Teofilović A, Vojnović-Milutinović D, Miladinović N, Kovačević S, Veličković N, Đorđević A. Macrophage migration inhibitory factor deficiency aggravates effects of fructose‐enriched diet on lipid metabolism in the mouse liver. in BioFactors. 2021;:biof.1711.
doi:10.1002/biof.1711 .
Gligorovska, Ljupka, Teofilović, Ana, Vojnović-Milutinović, Danijela, Miladinović, Nenad, Kovačević, Sanja, Veličković, Nataša, Đorđević, Ana, "Macrophage migration inhibitory factor deficiency aggravates effects of fructose‐enriched diet on lipid metabolism in the mouse liver" in BioFactors (2021):biof.1711,
https://doi.org/10.1002/biof.1711 . .
5
1
4

Decreased Glucocorticoid Signaling Potentiates Lipid-Induced Inflammation and Contributes to Insulin Resistance in the Skeletal Muscle of Fructose-Fed Male Rats Exposed to Stress

Shirif, Abdulbaset Zidane; Kovačević, Sanja; Brkljačić, Jelena; Teofilović, Ana; Elaković, Ivana; Đorđević, Ana; Matić, Gordana

(Basel, Switzerland: MDPI, 2021)

TY  - JOUR
AU  - Shirif, Abdulbaset Zidane
AU  - Kovačević, Sanja
AU  - Brkljačić, Jelena
AU  - Teofilović, Ana
AU  - Elaković, Ivana
AU  - Đorđević, Ana
AU  - Matić, Gordana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4259
AB  - The modern lifestyle brings both excessive fructose consumption and daily exposure
to stress which could lead to metabolic disturbances and type 2 diabetes. Muscles are important
points of glucose and lipid metabolism, with a crucial role in the maintenance of systemic energy
homeostasis. We investigated whether 9-week fructose-enriched diet, with and without exposure to 4-
week unpredictable stress, disturbs insulin signaling in the skeletal muscle of male rats and evaluated
potential contributory roles of muscle lipid metabolism, glucocorticoid signaling and inflammation.
The combination of fructose-enriched diet and stress increased peroxisome proliferator-activated
receptors-  and -  and stimulated lipid uptake, lipolysis and  -oxidation in the muscle of fructosefed
stressed rats. Combination of treatment also decreased systemic insulin sensitivity judged by
lower R-QUICKI, and lowered muscle protein content and stimulatory phosphorylations of insulin
receptor supstrate-1 and Akt, as well as the level of 11 -hydroxysteroid dehydrogenase type 1
and glucocorticoid receptor. At the same time, increased levels of protein tyrosine phosphatase-1B,
nuclear factor- B, tumor necrosis factor- , were observed in the muscle of fructose-fed stressed rats.
Based on these results, we propose that decreased glucocorticoid signaling in the skeletal muscle
can make a setting for lipid-induced inflammation and the development of insulin resistance in
fructose-fed stressed rats.
PB  - Basel, Switzerland: MDPI
T2  - International Journal of Molecular Sciences, Special Issue Glucocorticoids and Metabolic Disorders
T1  - Decreased Glucocorticoid Signaling Potentiates Lipid-Induced Inflammation and Contributes to Insulin Resistance in the Skeletal Muscle of Fructose-Fed Male Rats Exposed to Stress
IS  - 13
VL  - 22
DO  - 10.3390/ijms22137206
SP  - 7206
ER  - 
@article{
author = "Shirif, Abdulbaset Zidane and Kovačević, Sanja and Brkljačić, Jelena and Teofilović, Ana and Elaković, Ivana and Đorđević, Ana and Matić, Gordana",
year = "2021",
abstract = "The modern lifestyle brings both excessive fructose consumption and daily exposure
to stress which could lead to metabolic disturbances and type 2 diabetes. Muscles are important
points of glucose and lipid metabolism, with a crucial role in the maintenance of systemic energy
homeostasis. We investigated whether 9-week fructose-enriched diet, with and without exposure to 4-
week unpredictable stress, disturbs insulin signaling in the skeletal muscle of male rats and evaluated
potential contributory roles of muscle lipid metabolism, glucocorticoid signaling and inflammation.
The combination of fructose-enriched diet and stress increased peroxisome proliferator-activated
receptors-  and -  and stimulated lipid uptake, lipolysis and  -oxidation in the muscle of fructosefed
stressed rats. Combination of treatment also decreased systemic insulin sensitivity judged by
lower R-QUICKI, and lowered muscle protein content and stimulatory phosphorylations of insulin
receptor supstrate-1 and Akt, as well as the level of 11 -hydroxysteroid dehydrogenase type 1
and glucocorticoid receptor. At the same time, increased levels of protein tyrosine phosphatase-1B,
nuclear factor- B, tumor necrosis factor- , were observed in the muscle of fructose-fed stressed rats.
Based on these results, we propose that decreased glucocorticoid signaling in the skeletal muscle
can make a setting for lipid-induced inflammation and the development of insulin resistance in
fructose-fed stressed rats.",
publisher = "Basel, Switzerland: MDPI",
journal = "International Journal of Molecular Sciences, Special Issue Glucocorticoids and Metabolic Disorders",
title = "Decreased Glucocorticoid Signaling Potentiates Lipid-Induced Inflammation and Contributes to Insulin Resistance in the Skeletal Muscle of Fructose-Fed Male Rats Exposed to Stress",
number = "13",
volume = "22",
doi = "10.3390/ijms22137206",
pages = "7206"
}
Shirif, A. Z., Kovačević, S., Brkljačić, J., Teofilović, A., Elaković, I., Đorđević, A.,& Matić, G.. (2021). Decreased Glucocorticoid Signaling Potentiates Lipid-Induced Inflammation and Contributes to Insulin Resistance in the Skeletal Muscle of Fructose-Fed Male Rats Exposed to Stress. in International Journal of Molecular Sciences, Special Issue Glucocorticoids and Metabolic Disorders
Basel, Switzerland: MDPI., 22(13), 7206.
https://doi.org/10.3390/ijms22137206
Shirif AZ, Kovačević S, Brkljačić J, Teofilović A, Elaković I, Đorđević A, Matić G. Decreased Glucocorticoid Signaling Potentiates Lipid-Induced Inflammation and Contributes to Insulin Resistance in the Skeletal Muscle of Fructose-Fed Male Rats Exposed to Stress. in International Journal of Molecular Sciences, Special Issue Glucocorticoids and Metabolic Disorders. 2021;22(13):7206.
doi:10.3390/ijms22137206 .
Shirif, Abdulbaset Zidane, Kovačević, Sanja, Brkljačić, Jelena, Teofilović, Ana, Elaković, Ivana, Đorđević, Ana, Matić, Gordana, "Decreased Glucocorticoid Signaling Potentiates Lipid-Induced Inflammation and Contributes to Insulin Resistance in the Skeletal Muscle of Fructose-Fed Male Rats Exposed to Stress" in International Journal of Molecular Sciences, Special Issue Glucocorticoids and Metabolic Disorders, 22, no. 13 (2021):7206,
https://doi.org/10.3390/ijms22137206 . .
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