TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrif.bg.ac.rs/uploads/files/strane/Fakultet/Izdavacka_delatnost/Zbornici_radova/XI%20Simpozijum%20sa%20medjunarodnim%20ucescem%20-%20Inovacije%20u%20ratarskoj%20i%20povrtarskoj%20proizvodnji.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6487
AB  - Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility.
AB  - Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
T1  - Potato minituber production by aeroponics: effects of plant origin and genotype
T1  - Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа
SP  - 31
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6487
ER  - 

@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility., Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia",
title = "Potato minituber production by aeroponics: effects of plant origin and genotype, Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа",
pages = "31-32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6487"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487

Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia. 2023;:31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Potato minituber production by aeroponics: effects of plant origin and genotype" in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia (2023):31-32,
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrores.agro.unibl.org/wp-content/uploads/2023/05/Book-of-Proceedings-AgroReS-2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6488
AB  - Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.
PB  - Banja Luka: University of Banja Luka, Faculty of Agriculture
C3  - Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
T1  - Effects of cultivar and plant origin on the aeroponic production of potato minitubers
DO  - 10.7251/ZARS2301009B
SP  - 9
EP  - 18
ER  - 

@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.",
publisher = "Banja Luka: University of Banja Luka, Faculty of Agriculture",
journal = "Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina",
title = "Effects of cultivar and plant origin on the aeroponic production of potato minitubers",
doi = "10.7251/ZARS2301009B",
pages = "9-18"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
Banja Luka: University of Banja Luka, Faculty of Agriculture., 9-18.
https://doi.org/10.7251/ZARS2301009B

Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina. 2023;:9-18.
doi:10.7251/ZARS2301009B .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Effects of cultivar and plant origin on the aeroponic production of potato minitubers" in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina (2023):9-18,
https://doi.org/10.7251/ZARS2301009B . .

TY  - JOUR
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5122
AB  - Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Potato aeroponics: effects of cultivar and plant origin on minituber production
IS  - 10
VL  - 8
DO  - 10.3390/horticulturae8100915
SP  - 915
ER  - 

@article{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2022",
abstract = "Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Potato aeroponics: effects of cultivar and plant origin on minituber production",
number = "10",
volume = "8",
doi = "10.3390/horticulturae8100915",
pages = "915"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J.,& Momčilović, I.. (2022). Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae
Basel: MDPI., 8(10), 915.
https://doi.org/10.3390/horticulturae8100915

Broćić Z, Oljača J, Pantelić D, Rudić J, Momčilović I. Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae. 2022;8(10):915.
doi:10.3390/horticulturae8100915 .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Potato aeroponics: effects of cultivar and plant origin on minituber production" in Horticulturae, 8, no. 10 (2022):915,
https://doi.org/10.3390/horticulturae8100915 . .

TY  - JOUR
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/5/372
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4979
AB  - Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants
IS  - 5
VL  - 8
DO  - 10.3390/horticulturae8050372
SP  - 372
ER  - 

@article{
author = "Rudić, Jelena and Pantelić, Danijel and Oljača, Jasmina and Momčilović, Ivana",
year = "2022",
abstract = "Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants",
number = "5",
volume = "8",
doi = "10.3390/horticulturae8050372",
pages = "372"
}

Rudić, J., Pantelić, D., Oljača, J.,& Momčilović, I.. (2022). Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants. in Horticulturae
Basel: MDPI., 8(5), 372.
https://doi.org/10.3390/horticulturae8050372

Rudić J, Pantelić D, Oljača J, Momčilović I. Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants. in Horticulturae. 2022;8(5):372.
doi:10.3390/horticulturae8050372 .

Rudić, Jelena, Pantelić, Danijel, Oljača, Jasmina, Momčilović, Ivana, "Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants" in Horticulturae, 8, no. 5 (2022):372,
https://doi.org/10.3390/horticulturae8050372 . .

TY  - JOUR
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Momčilović, Ivana
AU  - Simonović, Ana
AU  - Pantelić, Danijel
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/3/488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4875
AB  - Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.
T2  - Antioxidants
T1  - In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression
IS  - 3
VL  - 11
DO  - 10.3390/antiox11030488
SP  - 488
ER  - 

@article{
author = "Rudić, Jelena and Dragićević, Milan and Momčilović, Ivana and Simonović, Ana and Pantelić, Danijel",
year = "2022",
abstract = "Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.",
journal = "Antioxidants",
title = "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression",
number = "3",
volume = "11",
doi = "10.3390/antiox11030488",
pages = "488"
}

Rudić, J., Dragićević, M., Momčilović, I., Simonović, A.,& Pantelić, D.. (2022). In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants, 11(3), 488.
https://doi.org/10.3390/antiox11030488

Rudić J, Dragićević M, Momčilović I, Simonović A, Pantelić D. In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants. 2022;11(3):488.
doi:10.3390/antiox11030488 .

Rudić, Jelena, Dragićević, Milan, Momčilović, Ivana, Simonović, Ana, Pantelić, Danijel, "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression" in Antioxidants, 11, no. 3 (2022):488,
https://doi.org/10.3390/antiox11030488 . .

TY  - CONF
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5124
AB  - Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5124
ER  - 

@conference{
editor = "Milutinović, Milica",
author = "Rudić, Jelena and Pantelić, Danijel and Momčilović, Ivana",
year = "2022",
abstract = "Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5124"
}

Milutinović, M., Rudić, J., Pantelić, D.,& Momčilović, I.. (2022). Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5124

Milutinović M, Rudić J, Pantelić D, Momčilović I. Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5124 .

Milutinović, Milica, Rudić, Jelena, Pantelić, Danijel, Momčilović, Ivana, "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L." in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5124 .

TY  - CONF
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5123
AB  - Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5123
ER  - 

@conference{
author = "Rudić, Jelena and Pantelić, Danijel and Momčilović, Ivana",
year = "2022",
abstract = "Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5123"
}

Rudić, J., Pantelić, D.,& Momčilović, I.. (2022). Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5123

Rudić J, Pantelić D, Momčilović I. Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5123 .

Rudić, Jelena, Pantelić, Danijel, Momčilović, Ivana, "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L." in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5123 .

TY  - CONF
AU  - Momčilović, Ivana
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4673
AB  - Potato, Solanum tuberosum L., is the most important non-grain food crop in the world and the main vegetable crop in Serbia. This species produces more food per unit of 
production area than any cereal crop and significantly contributes to global food security.
However, potato is highly susceptible to two major abiotic stresses: heat stress and drought.
These stresses may significantly affect the growth of potato plants at each of the developmental stages they emerge, ultimately causing a reduction in plant productivity due to the
smaller number of the formed tubers and lower tuber mass. High temperatures and/or water deficit also cause various tuber disorders, such as shape deformation, growth cracks,
secondary tuberization and skin netting or russeting, decreasing the proportion of marketable tubers. One of the disorders associated with heat stress accompanied by drought is
“sugar ends”, which adversely affects the processing and nutritional qualities of the tubers.
Due to global climate change, and the continuous rise in the Earth’s surface temperatures,
the detrimental effects of heat stress and drought on potato are intensifying. The global average surface temperature had already increased by 1.2 °C above pre-industrial levels, and
it will likely reach 1.5 °C by the year 2030. The impact of climate change will differ between
regions. At lower latitudes, especially in seasonally dry and tropical regions, potato productivity is projected to decrease even for small local temperature increases. By contrast, the
impact of climate change on potato yields might be beneficial for growing potatoes at higher
altitudes and higher latitudes. Nevertheless, a decline in global potato yields between 18-
32% by the year 2050 is expected, according to simulation-based analysis. World population
growth requires a significant increase in potato production, and solutions for mitigating the
damage of major abiotic stresses on potato productivity and quality are needed. The adverse
effects of heat stress and drought on potato can be alleviated to a certain extend by a shift
in planting time and application of cultivation technologies such as irrigation, mulching,
plant shading, intercropping, and plant growth regulator treatments. However, a principal
approach to mitigate damage is the development of heat- and drought-tolerant varieties. In
the past decades, significant progress was made towards understanding the mechanisms associated with potato heat- and drought-susceptibility, while the breeders’ efforts intensified
to select new, tolerant potato genotypes.
PB  - Belgrade: Serbian Nutrition Society
C3  - 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia
T1  - Impact of climate changes on potato production and mitigation strategies
SP  - 66
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4673
ER  - 

@conference{
author = "Momčilović, Ivana and Pantelić, Danijel and Rudić, Jelena",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the most important non-grain food crop in the world and the main vegetable crop in Serbia. This species produces more food per unit of 
production area than any cereal crop and significantly contributes to global food security.
However, potato is highly susceptible to two major abiotic stresses: heat stress and drought.
These stresses may significantly affect the growth of potato plants at each of the developmental stages they emerge, ultimately causing a reduction in plant productivity due to the
smaller number of the formed tubers and lower tuber mass. High temperatures and/or water deficit also cause various tuber disorders, such as shape deformation, growth cracks,
secondary tuberization and skin netting or russeting, decreasing the proportion of marketable tubers. One of the disorders associated with heat stress accompanied by drought is
“sugar ends”, which adversely affects the processing and nutritional qualities of the tubers.
Due to global climate change, and the continuous rise in the Earth’s surface temperatures,
the detrimental effects of heat stress and drought on potato are intensifying. The global average surface temperature had already increased by 1.2 °C above pre-industrial levels, and
it will likely reach 1.5 °C by the year 2030. The impact of climate change will differ between
regions. At lower latitudes, especially in seasonally dry and tropical regions, potato productivity is projected to decrease even for small local temperature increases. By contrast, the
impact of climate change on potato yields might be beneficial for growing potatoes at higher
altitudes and higher latitudes. Nevertheless, a decline in global potato yields between 18-
32% by the year 2050 is expected, according to simulation-based analysis. World population
growth requires a significant increase in potato production, and solutions for mitigating the
damage of major abiotic stresses on potato productivity and quality are needed. The adverse
effects of heat stress and drought on potato can be alleviated to a certain extend by a shift
in planting time and application of cultivation technologies such as irrigation, mulching,
plant shading, intercropping, and plant growth regulator treatments. However, a principal
approach to mitigate damage is the development of heat- and drought-tolerant varieties. In
the past decades, significant progress was made towards understanding the mechanisms associated with potato heat- and drought-susceptibility, while the breeders’ efforts intensified
to select new, tolerant potato genotypes.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia",
title = "Impact of climate changes on potato production and mitigation strategies",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4673"
}

Momčilović, I., Pantelić, D.,& Rudić, J.. (2021). Impact of climate changes on potato production and mitigation strategies. in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 66-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4673

Momčilović I, Pantelić D, Rudić J. Impact of climate changes on potato production and mitigation strategies. in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia. 2021;:66-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4673 .

Momčilović, Ivana, Pantelić, Danijel, Rudić, Jelena, "Impact of climate changes on potato production and mitigation strategies" in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia (2021):66-66,
https://hdl.handle.net/21.15107/rcub_ibiss_4673 .

TY  - CHAP
AU  - Momčilović, Ivana
AU  - Fu, Jianming
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Broćić, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4470
AB  - Potato, Solanum tuberosum L., is the most important non-grain food crop in the world, which produces more food per unit of production area than any cereal crop and significantly contributes to global food security. Potato domestication originated in a cool climate region of the Andes and, due to its narrow genetic basis of heat tolerance traits, this vegetable species is highly susceptible to heat stress. High-temperature stress delays sprout emergence, reduces leaf size, total leaf area, the number of stolons, the number and mass of tubers, and causes tuber disorders, consequently reducing the total and marketable yield of potato. Heat stress also negatively affects the nutritive and processing qualities of tubers. Due to global climate change, and the continuous rise in the Earth's surface temperatures, the detrimental effects of heat stress on potato crop are intensifying. At the same time, world population growth requires a significant increase in potato production. Thus, solutions for mitigating the damage of high-temperature stress on potato productivity are essential. In the past decades, various researches on the effects of high temperatures on potato plants have been conducted, and significant progress towards understanding the mechanisms underlying potato heat stress responses at the whole plant and molecular levels has been made. This chapter summarizes the current knowledge of the effects of heat stress on potato growth, development, productivity and nutritive qualities, and mechanisms associated with potato heat susceptibility. Future research directions and the development of potato varieties with improved heat tolerance are also discussed.
PB  - New York : Nova Science Publishers Inc.
T2  - The Potato Crop: Management, Production, and Food Security
T1  - Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement
DO  - 10.52305/RHLO1469
SP  - 91
EP  - 122
ER  - 

@inbook{
editor = "Villa, Pedro",
author = "Momčilović, Ivana and Fu, Jianming and Pantelić, Danijel and Rudić, Jelena and Broćić, Zoran",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the most important non-grain food crop in the world, which produces more food per unit of production area than any cereal crop and significantly contributes to global food security. Potato domestication originated in a cool climate region of the Andes and, due to its narrow genetic basis of heat tolerance traits, this vegetable species is highly susceptible to heat stress. High-temperature stress delays sprout emergence, reduces leaf size, total leaf area, the number of stolons, the number and mass of tubers, and causes tuber disorders, consequently reducing the total and marketable yield of potato. Heat stress also negatively affects the nutritive and processing qualities of tubers. Due to global climate change, and the continuous rise in the Earth's surface temperatures, the detrimental effects of heat stress on potato crop are intensifying. At the same time, world population growth requires a significant increase in potato production. Thus, solutions for mitigating the damage of high-temperature stress on potato productivity are essential. In the past decades, various researches on the effects of high temperatures on potato plants have been conducted, and significant progress towards understanding the mechanisms underlying potato heat stress responses at the whole plant and molecular levels has been made. This chapter summarizes the current knowledge of the effects of heat stress on potato growth, development, productivity and nutritive qualities, and mechanisms associated with potato heat susceptibility. Future research directions and the development of potato varieties with improved heat tolerance are also discussed.",
publisher = "New York : Nova Science Publishers Inc.",
journal = "The Potato Crop: Management, Production, and Food Security",
booktitle = "Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement",
doi = "10.52305/RHLO1469",
pages = "91-122"
}

Villa, P., Momčilović, I., Fu, J., Pantelić, D., Rudić, J.,& Broćić, Z.. (2021). Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement. in The Potato Crop: Management, Production, and Food Security
New York : Nova Science Publishers Inc.., 91-122.
https://doi.org/10.52305/RHLO1469

Villa P, Momčilović I, Fu J, Pantelić D, Rudić J, Broćić Z. Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement. in The Potato Crop: Management, Production, and Food Security. 2021;:91-122.
doi:10.52305/RHLO1469 .

Villa, Pedro, Momčilović, Ivana, Fu, Jianming, Pantelić, Danijel, Rudić, Jelena, Broćić, Zoran, "Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement" in The Potato Crop: Management, Production, and Food Security (2021):91-122,
https://doi.org/10.52305/RHLO1469 . .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2021
UR  - https://www.vibconferences.be/events/plant-science-for-climate-emergency
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4340
AB  - Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.
PB  - VIB Conferences Series
C3  - Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
T1  - The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression
SP  - 97 / P38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4340
ER  - 

@conference{
author = "Pantelić, Danijel and Rudić, Jelena and Dragićević, Milan and Simonović, Ana and Momčilović, Ivana",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.",
publisher = "VIB Conferences Series",
journal = "Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.",
title = "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression",
pages = "97 / P38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4340"
}

Pantelić, D., Rudić, J., Dragićević, M., Simonović, A.,& Momčilović, I.. (2021). The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
VIB Conferences Series., 97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340

Pantelić D, Rudić J, Dragićević M, Simonović A, Momčilović I. The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.. 2021;:97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

Pantelić, Danijel, Rudić, Jelena, Dragićević, Milan, Simonović, Ana, Momčilović, Ivana, "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression" in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97. (2021):97 / P38,
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Dragićević, Milan
AU  - Pantelić, Danijel
AU  - Milošević, Snežana
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1589
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4009
AB  - Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.
PB  - MDPI AG
T2  - Plants
T1  - Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana
IS  - 11
VL  - 9
DO  - 10.3390/plants9111589
SP  - 1589
ER  - 

@article{
author = "Antonić Reljin, Dragana and Subotić, Angelina and Dragićević, Milan and Pantelić, Danijel and Milošević, Snežana and Simonović, Ana and Momčilović, Ivana",
year = "2020",
abstract = "Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana",
number = "11",
volume = "9",
doi = "10.3390/plants9111589",
pages = "1589"
}

Antonić Reljin, D., Subotić, A., Dragićević, M., Pantelić, D., Milošević, S., Simonović, A.,& Momčilović, I.. (2020). Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants
MDPI AG., 9(11), 1589.
https://doi.org/10.3390/plants9111589

Antonić Reljin D, Subotić A, Dragićević M, Pantelić D, Milošević S, Simonović A, Momčilović I. Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants. 2020;9(11):1589.
doi:10.3390/plants9111589 .

Antonić Reljin, Dragana, Subotić, Angelina, Dragićević, Milan, Pantelić, Danijel, Milošević, Snežana, Simonović, Ana, Momčilović, Ivana, "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana" in Plants, 9, no. 11 (2020):1589,
https://doi.org/10.3390/plants9111589 . .

TY  - CONF
AU  - Marković, Stefan M.
AU  - Djukić, Nevena H.
AU  - Knežević, Desimir
AU  - Pantelić, Danijel
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4350
AB  - High temperature stress is one of the most important environmental factors that influence cereal’s growth, development and yield processes. For this reason, it is important to identify proteins involved in heat stress response of cereals and develop varieties tolerant to high temperatures.Recent studies haveshown that accumulation of eukaryotic elongation factor 1A (eEF1A) plays a role in heat tolerancein wheat.The aim of this research was to examine the impact of heat stress on accumulation of eEF1A in several cereals and to compare relative abundance of eEF1A in different cereal varieties.Flag leaves of four cereal varieties were sampled and used for research. After the isolation of proteins, immunoblot analysis was conducted foreEF1A quantification1 .The results showed differences among analyzed cereal varieties according to accumulation of eEF1A. Heatinduced accumulation of eEF1A was shown inthree investigated cereal varieties.The highest accumulationof eEF1A underheat-stress condition was found in wheat variety Anapurna. On the other hand, a decline in the relative abundance of eEF1A was shown in wheat variety Avenu under the stress condition. According to these findings, we can recommend wheat variety Anapurna for breeding new wheat with improved adaptability to high temperatures.
PB  - Belgrade: Serbian Biochemical Society
C3  - The 9th Conference of the Serbian Biochemical Society: “Diversity in Biochemistry”
T1  - Impact of high temperature on the accumulation of eEF1A in different cereal varieties
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4350
ER  - 

@conference{
author = "Marković, Stefan M. and Djukić, Nevena H. and Knežević, Desimir and Pantelić, Danijel",
year = "2019",
abstract = "High temperature stress is one of the most important environmental factors that influence cereal’s growth, development and yield processes. For this reason, it is important to identify proteins involved in heat stress response of cereals and develop varieties tolerant to high temperatures.Recent studies haveshown that accumulation of eukaryotic elongation factor 1A (eEF1A) plays a role in heat tolerancein wheat.The aim of this research was to examine the impact of heat stress on accumulation of eEF1A in several cereals and to compare relative abundance of eEF1A in different cereal varieties.Flag leaves of four cereal varieties were sampled and used for research. After the isolation of proteins, immunoblot analysis was conducted foreEF1A quantification1 .The results showed differences among analyzed cereal varieties according to accumulation of eEF1A. Heatinduced accumulation of eEF1A was shown inthree investigated cereal varieties.The highest accumulationof eEF1A underheat-stress condition was found in wheat variety Anapurna. On the other hand, a decline in the relative abundance of eEF1A was shown in wheat variety Avenu under the stress condition. According to these findings, we can recommend wheat variety Anapurna for breeding new wheat with improved adaptability to high temperatures.",
publisher = "Belgrade: Serbian Biochemical Society",
journal = "The 9th Conference of the Serbian Biochemical Society: “Diversity in Biochemistry”",
title = "Impact of high temperature on the accumulation of eEF1A in different cereal varieties",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4350"
}

Marković, S. M., Djukić, N. H., Knežević, D.,& Pantelić, D.. (2019). Impact of high temperature on the accumulation of eEF1A in different cereal varieties. in The 9th Conference of the Serbian Biochemical Society: “Diversity in Biochemistry”
Belgrade: Serbian Biochemical Society., 123.
https://hdl.handle.net/21.15107/rcub_ibiss_4350

Marković SM, Djukić NH, Knežević D, Pantelić D. Impact of high temperature on the accumulation of eEF1A in different cereal varieties. in The 9th Conference of the Serbian Biochemical Society: “Diversity in Biochemistry”. 2019;:123.
https://hdl.handle.net/21.15107/rcub_ibiss_4350 .

Marković, Stefan M., Djukić, Nevena H., Knežević, Desimir, Pantelić, Danijel, "Impact of high temperature on the accumulation of eEF1A in different cereal varieties" in The 9th Conference of the Serbian Biochemical Society: “Diversity in Biochemistry” (2019):123,
https://hdl.handle.net/21.15107/rcub_ibiss_4350 .

TY  - JOUR
AU  - Đukić, Nevena
AU  - Knežević, Desimir
AU  - Pantelić, Danijel
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
AU  - Marković, Stefan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S017616171930118X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3446
AB  - The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23 °C; MT) and high air temperature (38 °C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heat-stress conditions.
T2  - Journal of Plant Physiology
T1  - Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress.
VL  - 240
DO  - 10.1016/j.jplph.2019.153015
SP  - 153015
ER  - 

@article{
author = "Đukić, Nevena and Knežević, Desimir and Pantelić, Danijel and Živančev, Dragan and Torbica, Aleksandra and Marković, Stefan",
year = "2019",
abstract = "The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23 °C; MT) and high air temperature (38 °C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heat-stress conditions.",
journal = "Journal of Plant Physiology",
title = "Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress.",
volume = "240",
doi = "10.1016/j.jplph.2019.153015",
pages = "153015"
}

Đukić, N., Knežević, D., Pantelić, D., Živančev, D., Torbica, A.,& Marković, S.. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress.. in Journal of Plant Physiology, 240, 153015.
https://doi.org/10.1016/j.jplph.2019.153015

Đukić N, Knežević D, Pantelić D, Živančev D, Torbica A, Marković S. Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress.. in Journal of Plant Physiology. 2019;240:153015.
doi:10.1016/j.jplph.2019.153015 .

Đukić, Nevena, Knežević, Desimir, Pantelić, Danijel, Živančev, Dragan, Torbica, Aleksandra, Marković, Stefan, "Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress." in Journal of Plant Physiology, 240 (2019):153015,
https://doi.org/10.1016/j.jplph.2019.153015 . .

TY  - THES
AU  - Pantelić, Danijel
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3259
AB  - Eukariotski elongacioni faktor translacije 1A (eEF1A) je citosolni, multifunkcionalni protein koji ima ključnu ulogu u elongacionoj fazi tokom sinteze proteina, vršeći transport aa-tRNK do mesta A na ribozomima. Pored osnovne funkcije, ovaj elongacioni faktor može učestvovati i u eksportu molekula iz nukleusa, organizaciji citoskeleta, proteolitičkoj degradaciji proteina, kao i posedovati aktivnost molekularnog šaperona. U ovoj tezi ispitan je uticaj visoke temperature na proces tuberizacije, rastenje i razviće, kao i ekspresiju eEF1A u izdancima, listovima i krtolama kod različitih sorti krompira gajenih u kontrolisanim uslovima ili u polju. Pored toga, urađena je i karakterizacija izoformi eEF1A u listovima kod različitih sorti krompira gajenih ex vitro ili u polju. Kod različitih sorti krompira izvršena je procena broja kopija gena koji kodiraju eEF1A, a ekspresija eEF1A gena u uslovima visoke temperature je ispitana kod sorti Désirée i Festival. Analiza zastupljenost eEF1A u mikro- i minikrtolama kod toplotno-osetljive sorte Désirée, je pokazala da u uslovima dugotrajnog toplotnog stresa dolazi do smanjenja zastupljenosti eEF1A, dok sa druge strane kod toplotno-tolerantne sorte Festival, dolazi do povećanja zastupljenosti eEF1A, kako u mikrokrtolama, tako i u starijim minikrtolama. Ovaj rezultat ukazuje na ulogu eEF1A u ublažavanju negativnog uticaja visoke temperature na proces tuberizacije kod krompira. Značajna korelacija ustanovljena je između zastupljenosti eEF1A u listovima krompira gajenih u polju tokom dve izuzetno tople godine (2011. i 2012.) i prinosa krompira. Rezultati eksperimenata u uslovima ex vitro, kao i polju, su pokazali da je relativna molekulska masa eEF1A krompira 49,2 kDa i da ima 5-8 izoformi. Povećanoj zastupljenosti eEF1A u uslovima visoke temperature, najviše su doprinela 2-3 polipeptida/izoforme iz bazne grupe. Genomska analiza je ukazala na postojanje više gena koji kodiraju eEF1A kod krompira, kao i da se ispitivane sorte razlikuju po broju kopija gena. Analiza ekspresije eEF1A gena u minikrtolama toplotno-osetljive sorte Désirée pokazala da je ekspresija većine gena osetljiva na delovanje visoke temperature, te dolazi do značajnog smanjenjenja broja većine eEF1A transkripata u uslovima toplotnog stresa. Sa druge strane, kod toplotno-tolerantne sorte Festival, visoka temperatura je imala blaži efekat na ekspresiju većine eEF1A gena, odnosno zastupljenost pojedinih transkripata se blago povećala ili ostala na nivou kontrole u uslovima visoke temperature. Izuzetak je predstavljao transkript eEF1A-12 gena koji se karakterisao izuzetnom inducibilnošću. Na osnovu dobijenih rezultata može se zaključiti da ekspresija gena eEF1A-12 u uslovima visoke temperature najviše doprinosi povećanju zastupljenosti eEF1A proteina u krtolama toplotno-tolerantne sorte Festival, što otvara mogućnost za njegovo korišćenje u kreiranju novih, termotolerantnih sorti krompira putem klasičnih metoda selekcije ili metoda genetičkog inženjeringa.
AB  - Eukaryotic elongation factor 1A (eEF1A) is a cytosolic, multifunctional protein that plays a central role in the elongation phase of translation by delivering aa-tRNA complex to the A site of the ribosome. The eEF1A is also implicated in the nuclear export of aa-tRNA, organization of the cytoskeleton, participates in proteolysis, and possess chaperone activity. In this thesis, the effects of high temperatures on tuberization, growth, and development, as well as the expression and accumulation of eEF1A in shoots, leaves, and tubers, were investigated in different potato cultivars grown in a controlled environment and field. In addition, the characterization of eEF1A isoforms in leaves of different potato cultivars grown ex vitro or in the field was done. Also, in different potato cultivars, the number of eEF1A-coding genes, as well as the eEF1A genes' transcript expression under a high-temperature treatment, were investigated. Investigation of eEF1A accumulation in micro- and minitubers of heat-sensitive cultivar Désirée revealed a lower level of eEF1A under prolonged heat-stress, while in heat-tolerant cultivar Festival, a higher level of eEF1A in microtubers and older minitubers was found. Also, a significant positive correlation between eEF1A abundance in potato leaves and the potato productivity in the field was observed in two extremely hot years 2001. and 2012. These results indicate an important eEF1A role in mitigation of the adverse impacts of high temperature on the tuberization in potato. Results from ex vitro and field experiments have shown that potato eEF1A is a protein of 49.2 kDa with multiple isoforms (5-8). Increase in eEF1A abundance under high-temperature condition was mainly due to 2-3 basic polypeptides/isoforms. Genomic analysis indicated the existence of multiple genes encoding eEF1A in potato. Analysis of eEF1A genes' transcripts in minitubers of heat-sensitive cultivar Désirée revealed that expression of the most genes was sensitive to high temperature, and there was a significant reduction in number of eEF1A transcripts. On the other hand, in heat-tolerant cultivar Festival, high temperature had a milder effect on eEF1A gene-expression. The abundance of most eEF1A transcripts slightly increased or remained unchanged under high-temperature condition, with the exception of the eEF1A-12 transcript which was highly heat-inducible. Based on results obtained in this thesis, it can be concluded that eEF1A-12 gene expression mainly contributes to an observed increase in eEF1A abundance under high-temperature conditions in tubers of heat-tolerant cultivar Festival, which opens the possibility for its utilization in the development of new heat-tolerant potato varieties by classical breeding or genetic engineering.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres
T1  - Translation elongation factor 1A in potato (Solanum tuberosum L.): characterisation of isoforms, expression, and role in plant response to heat-stress
SP  - 1
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3259
ER  - 

@phdthesis{
author = "Pantelić, Danijel",
year = "2019",
abstract = "Eukariotski elongacioni faktor translacije 1A (eEF1A) je citosolni, multifunkcionalni protein koji ima ključnu ulogu u elongacionoj fazi tokom sinteze proteina, vršeći transport aa-tRNK do mesta A na ribozomima. Pored osnovne funkcije, ovaj elongacioni faktor može učestvovati i u eksportu molekula iz nukleusa, organizaciji citoskeleta, proteolitičkoj degradaciji proteina, kao i posedovati aktivnost molekularnog šaperona. U ovoj tezi ispitan je uticaj visoke temperature na proces tuberizacije, rastenje i razviće, kao i ekspresiju eEF1A u izdancima, listovima i krtolama kod različitih sorti krompira gajenih u kontrolisanim uslovima ili u polju. Pored toga, urađena je i karakterizacija izoformi eEF1A u listovima kod različitih sorti krompira gajenih ex vitro ili u polju. Kod različitih sorti krompira izvršena je procena broja kopija gena koji kodiraju eEF1A, a ekspresija eEF1A gena u uslovima visoke temperature je ispitana kod sorti Désirée i Festival. Analiza zastupljenost eEF1A u mikro- i minikrtolama kod toplotno-osetljive sorte Désirée, je pokazala da u uslovima dugotrajnog toplotnog stresa dolazi do smanjenja zastupljenosti eEF1A, dok sa druge strane kod toplotno-tolerantne sorte Festival, dolazi do povećanja zastupljenosti eEF1A, kako u mikrokrtolama, tako i u starijim minikrtolama. Ovaj rezultat ukazuje na ulogu eEF1A u ublažavanju negativnog uticaja visoke temperature na proces tuberizacije kod krompira. Značajna korelacija ustanovljena je između zastupljenosti eEF1A u listovima krompira gajenih u polju tokom dve izuzetno tople godine (2011. i 2012.) i prinosa krompira. Rezultati eksperimenata u uslovima ex vitro, kao i polju, su pokazali da je relativna molekulska masa eEF1A krompira 49,2 kDa i da ima 5-8 izoformi. Povećanoj zastupljenosti eEF1A u uslovima visoke temperature, najviše su doprinela 2-3 polipeptida/izoforme iz bazne grupe. Genomska analiza je ukazala na postojanje više gena koji kodiraju eEF1A kod krompira, kao i da se ispitivane sorte razlikuju po broju kopija gena. Analiza ekspresije eEF1A gena u minikrtolama toplotno-osetljive sorte Désirée pokazala da je ekspresija većine gena osetljiva na delovanje visoke temperature, te dolazi do značajnog smanjenjenja broja većine eEF1A transkripata u uslovima toplotnog stresa. Sa druge strane, kod toplotno-tolerantne sorte Festival, visoka temperatura je imala blaži efekat na ekspresiju većine eEF1A gena, odnosno zastupljenost pojedinih transkripata se blago povećala ili ostala na nivou kontrole u uslovima visoke temperature. Izuzetak je predstavljao transkript eEF1A-12 gena koji se karakterisao izuzetnom inducibilnošću. Na osnovu dobijenih rezultata može se zaključiti da ekspresija gena eEF1A-12 u uslovima visoke temperature najviše doprinosi povećanju zastupljenosti eEF1A proteina u krtolama toplotno-tolerantne sorte Festival, što otvara mogućnost za njegovo korišćenje u kreiranju novih, termotolerantnih sorti krompira putem klasičnih metoda selekcije ili metoda genetičkog inženjeringa., Eukaryotic elongation factor 1A (eEF1A) is a cytosolic, multifunctional protein that plays a central role in the elongation phase of translation by delivering aa-tRNA complex to the A site of the ribosome. The eEF1A is also implicated in the nuclear export of aa-tRNA, organization of the cytoskeleton, participates in proteolysis, and possess chaperone activity. In this thesis, the effects of high temperatures on tuberization, growth, and development, as well as the expression and accumulation of eEF1A in shoots, leaves, and tubers, were investigated in different potato cultivars grown in a controlled environment and field. In addition, the characterization of eEF1A isoforms in leaves of different potato cultivars grown ex vitro or in the field was done. Also, in different potato cultivars, the number of eEF1A-coding genes, as well as the eEF1A genes' transcript expression under a high-temperature treatment, were investigated. Investigation of eEF1A accumulation in micro- and minitubers of heat-sensitive cultivar Désirée revealed a lower level of eEF1A under prolonged heat-stress, while in heat-tolerant cultivar Festival, a higher level of eEF1A in microtubers and older minitubers was found. Also, a significant positive correlation between eEF1A abundance in potato leaves and the potato productivity in the field was observed in two extremely hot years 2001. and 2012. These results indicate an important eEF1A role in mitigation of the adverse impacts of high temperature on the tuberization in potato. Results from ex vitro and field experiments have shown that potato eEF1A is a protein of 49.2 kDa with multiple isoforms (5-8). Increase in eEF1A abundance under high-temperature condition was mainly due to 2-3 basic polypeptides/isoforms. Genomic analysis indicated the existence of multiple genes encoding eEF1A in potato. Analysis of eEF1A genes' transcripts in minitubers of heat-sensitive cultivar Désirée revealed that expression of the most genes was sensitive to high temperature, and there was a significant reduction in number of eEF1A transcripts. On the other hand, in heat-tolerant cultivar Festival, high temperature had a milder effect on eEF1A gene-expression. The abundance of most eEF1A transcripts slightly increased or remained unchanged under high-temperature condition, with the exception of the eEF1A-12 transcript which was highly heat-inducible. Based on results obtained in this thesis, it can be concluded that eEF1A-12 gene expression mainly contributes to an observed increase in eEF1A abundance under high-temperature conditions in tubers of heat-tolerant cultivar Festival, which opens the possibility for its utilization in the development of new heat-tolerant potato varieties by classical breeding or genetic engineering.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres, Translation elongation factor 1A in potato (Solanum tuberosum L.): characterisation of isoforms, expression, and role in plant response to heat-stress",
pages = "1-142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3259"
}

Pantelić, D.. (2019). Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-142.
https://hdl.handle.net/21.15107/rcub_ibiss_3259

Pantelić D. Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres. in University of Belgrade, Faculty of Biology. 2019;:1-142.
https://hdl.handle.net/21.15107/rcub_ibiss_3259 .

Pantelić, Danijel, "Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres" in University of Belgrade, Faculty of Biology (2019):1-142,
https://hdl.handle.net/21.15107/rcub_ibiss_3259 .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Oljača, Jasmina
AU  - Simonović, Ana
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4348
AB  - Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance
SP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4348
ER  - 

@conference{
editor = "Kovačević, Dušan",
author = "Pantelić, Danijel and Rudić, Jelena and Oljača, Jasmina and Simonović, Ana and Broćić, Zoran and Momčilović, Ivana",
year = "2018",
abstract = "Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance",
pages = "335",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4348"
}

Kovačević, D., Pantelić, D., Rudić, J., Oljača, J., Simonović, A., Broćić, Z.,& Momčilović, I.. (2018). Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348

Kovačević D, Pantelić D, Rudić J, Oljača J, Simonović A, Broćić Z, Momčilović I. Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2018;:335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

Kovačević, Dušan, Pantelić, Danijel, Rudić, Jelena, Oljača, Jasmina, Simonović, Ana, Broćić, Zoran, Momčilović, Ivana, "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance" in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina (2018):335,
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

TY  - CONF
AU  - Oljača, Jasmina
AU  - Broćič, Zoran
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4349
AB  - Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - IX International Scientific Agriculture Symposium “Agrosym 2018”
T1  - Prospective protein markers for selection of heat tolerant potato cultivars
SP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4349
ER  - 

@conference{
author = "Oljača, Jasmina and Broćič, Zoran and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2018",
abstract = "Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "IX International Scientific Agriculture Symposium “Agrosym 2018”",
title = "Prospective protein markers for selection of heat tolerant potato cultivars",
pages = "340",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4349"
}

Oljača, J., Broćič, Z., Pantelić, D., Rudić, J.,& Momčilović, I.. (2018). Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349

Oljača J, Broćič Z, Pantelić D, Rudić J, Momčilović I. Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”. 2018;:340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

Oljača, Jasmina, Broćič, Zoran, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Prospective protein markers for selection of heat tolerant potato cultivars" in IX International Scientific Agriculture Symposium “Agrosym 2018” (2018):340,
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

TY  - JOUR
AU  - Pantelić, Danijel
AU  - Dragićević, Ivana Č.
AU  - Rudić, Jelena
AU  - Fu, Jianming
AU  - Momčilović, Ivana
PY  - 2018
UR  - http://link.springer.com/10.1007/s13580-018-0043-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3089
AB  - Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.
T2  - Horticulture, Environment, and Biotechnology
T1  - Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato
IS  - 3
VL  - 59
DO  - 10.1007/s13580-018-0043-x
SP  - 315
EP  - 324
ER  - 

@article{
author = "Pantelić, Danijel and Dragićević, Ivana Č. and Rudić, Jelena and Fu, Jianming and Momčilović, Ivana",
year = "2018",
abstract = "Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.",
journal = "Horticulture, Environment, and Biotechnology",
title = "Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato",
number = "3",
volume = "59",
doi = "10.1007/s13580-018-0043-x",
pages = "315-324"
}

Pantelić, D., Dragićević, I. Č., Rudić, J., Fu, J.,& Momčilović, I.. (2018). Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. in Horticulture, Environment, and Biotechnology, 59(3), 315-324.
https://doi.org/10.1007/s13580-018-0043-x

Pantelić D, Dragićević IČ, Rudić J, Fu J, Momčilović I. Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. in Horticulture, Environment, and Biotechnology. 2018;59(3):315-324.
doi:10.1007/s13580-018-0043-x .

Pantelić, Danijel, Dragićević, Ivana Č., Rudić, Jelena, Fu, Jianming, Momčilović, Ivana, "Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato" in Horticulture, Environment, and Biotechnology, 59, no. 3 (2018):315-324,
https://doi.org/10.1007/s13580-018-0043-x . .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Pantelić, Danijel
AU  - Simonović, Ana
AU  - Trajković, Milena
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4337
AB  - Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4337
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Pantelić, Danijel and Simonović, Ana and Trajković, Milena and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4337"
}

Antonić, D., Milošević, S., Pantelić, D., Simonović, A., Trajković, M., Momčilović, I.,& Subotić, A.. (2018). Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337

Antonić D, Milošević S, Pantelić D, Simonović A, Trajković M, Momčilović I, Subotić A. Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

Antonić, Dragana, Milošević, Snežana, Pantelić, Danijel, Simonović, Ana, Trajković, Milena, Momčilović, Ivana, Subotić, Angelina, "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

TY  - CHAP
AU  - Momčilović, Ivana
AU  - Pantelić, Danijel
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3029
AB  - Abiotic stresses affect plant growth and development, and significantly reduce crop productivity by causing the alteration in cell biochemistry. Common for all abiotic stresses is that they cause an imbalance between production and scavenging of reactive oxygen species (ROS) in cells, resulting in elevation of ROS concentrations to cytotoxic level. Superoxide dismutases (SODs) are enzymes that play an important role of primary ROS scavengers by catalyzing the conversion of superoxide anion radical to hydrogen peroxide and oxygen. Most plant species contain numerous SOD isoforms which are classified according to the active site metal into three major types: FeSOD, MnSOD and Cu/ZnSOD. All plant SODs are nuclear-encoded, synthesized on cytosolic ribosomes and transported into various cell compartments, such as cytosol, plastids, mitochondria, peroxisomes and cell wall. Expression of SODs genes might be modulated by developmental and environmental cues, including nutrition and abiotic stresses. So far, enhanced expression and/or activity of SODs have been reported in response to various abiotic stressors, such as water deficit, high salinity, heavy metals, hypoxia, high and low temperatures. This chapter summarizes the current knowledge of plant SODs, their abiotic-stress modulated expression and activity, and analyses results on genetic engineering of plant SODs. The significance of superoxide dismutases in the crop improvement for stress tolerance is also discussed.
PB  - Nova Science Publishers, Inc.
T2  - Superoxide Dismutase: Structure, Synthesis and Applications
T1  - Plant superoxide dismutases: Important players in abiotic stress tolerance
SP  - 45
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3029
ER  - 

@inbook{
author = "Momčilović, Ivana and Pantelić, Danijel",
year = "2018",
abstract = "Abiotic stresses affect plant growth and development, and significantly reduce crop productivity by causing the alteration in cell biochemistry. Common for all abiotic stresses is that they cause an imbalance between production and scavenging of reactive oxygen species (ROS) in cells, resulting in elevation of ROS concentrations to cytotoxic level. Superoxide dismutases (SODs) are enzymes that play an important role of primary ROS scavengers by catalyzing the conversion of superoxide anion radical to hydrogen peroxide and oxygen. Most plant species contain numerous SOD isoforms which are classified according to the active site metal into three major types: FeSOD, MnSOD and Cu/ZnSOD. All plant SODs are nuclear-encoded, synthesized on cytosolic ribosomes and transported into various cell compartments, such as cytosol, plastids, mitochondria, peroxisomes and cell wall. Expression of SODs genes might be modulated by developmental and environmental cues, including nutrition and abiotic stresses. So far, enhanced expression and/or activity of SODs have been reported in response to various abiotic stressors, such as water deficit, high salinity, heavy metals, hypoxia, high and low temperatures. This chapter summarizes the current knowledge of plant SODs, their abiotic-stress modulated expression and activity, and analyses results on genetic engineering of plant SODs. The significance of superoxide dismutases in the crop improvement for stress tolerance is also discussed.",
publisher = "Nova Science Publishers, Inc.",
journal = "Superoxide Dismutase: Structure, Synthesis and Applications",
booktitle = "Plant superoxide dismutases: Important players in abiotic stress tolerance",
pages = "45-100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3029"
}

Momčilović, I.,& Pantelić, D.. (2018). Plant superoxide dismutases: Important players in abiotic stress tolerance. in Superoxide Dismutase: Structure, Synthesis and Applications
Nova Science Publishers, Inc.., 45-100.
https://hdl.handle.net/21.15107/rcub_ibiss_3029

Momčilović I, Pantelić D. Plant superoxide dismutases: Important players in abiotic stress tolerance. in Superoxide Dismutase: Structure, Synthesis and Applications. 2018;:45-100.
https://hdl.handle.net/21.15107/rcub_ibiss_3029 .

Momčilović, Ivana, Pantelić, Danijel, "Plant superoxide dismutases: Important players in abiotic stress tolerance" in Superoxide Dismutase: Structure, Synthesis and Applications (2018):45-100,
https://hdl.handle.net/21.15107/rcub_ibiss_3029 .