TY  - JOUR
AU  - Stegnjaić, Goran
AU  - Jevtić, Bojan
AU  - Lazarević, Milica
AU  - Ignjatović, Đurđica
AU  - Tomić, Mirko
AU  - Nikolovski, Neda
AU  - Bjelobaba, Ivana
AU  - Momčilović, Miljana
AU  - Dimitrijević, Mirjana
AU  - Miljković, Đorđe
AU  - Stanisavljević, Suzana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6643
AB  - We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund’s adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of T and B cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. T cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.
PB  - Elsevier
T2  - Immunology Letters
T1  - Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate
VL  - 267
DO  - 10.1016/j.imlet.2024.106852
SP  - 106852
ER  - 

@article{
author = "Stegnjaić, Goran and Jevtić, Bojan and Lazarević, Milica and Ignjatović, Đurđica and Tomić, Mirko and Nikolovski, Neda and Bjelobaba, Ivana and Momčilović, Miljana and Dimitrijević, Mirjana and Miljković, Đorđe and Stanisavljević, Suzana",
year = "2024",
abstract = "We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund’s adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of T and B cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. T cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.",
publisher = "Elsevier",
journal = "Immunology Letters",
title = "Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate",
volume = "267",
doi = "10.1016/j.imlet.2024.106852",
pages = "106852"
}

Stegnjaić, G., Jevtić, B., Lazarević, M., Ignjatović, Đ., Tomić, M., Nikolovski, N., Bjelobaba, I., Momčilović, M., Dimitrijević, M., Miljković, Đ.,& Stanisavljević, S.. (2024). Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate. in Immunology Letters
Elsevier., 267, 106852.
https://doi.org/10.1016/j.imlet.2024.106852

Stegnjaić G, Jevtić B, Lazarević M, Ignjatović Đ, Tomić M, Nikolovski N, Bjelobaba I, Momčilović M, Dimitrijević M, Miljković Đ, Stanisavljević S. Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate. in Immunology Letters. 2024;267:106852.
doi:10.1016/j.imlet.2024.106852 .

Stegnjaić, Goran, Jevtić, Bojan, Lazarević, Milica, Ignjatović, Đurđica, Tomić, Mirko, Nikolovski, Neda, Bjelobaba, Ivana, Momčilović, Miljana, Dimitrijević, Mirjana, Miljković, Đorđe, Stanisavljević, Suzana, "Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate" in Immunology Letters, 267 (2024):106852,
https://doi.org/10.1016/j.imlet.2024.106852 . .

TY  - JOUR
AU  - Stegnjaić, Goran
AU  - Jevtić, Bojan
AU  - Lazarević, Milica
AU  - Ignjatović, Đurđica
AU  - Tomić, Mirko
AU  - Nikolovski, Neda
AU  - Bjelobaba, Ivana
AU  - Momčilović, Miljana
AU  - Dimitrijević, Mirjana
AU  - Miljković, Đorđe
AU  - Stanisavljević, Suzana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6644
AB  - We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund’s adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of T and B cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. T cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.
PB  - Elsevier
T2  - Immunology Letters
T1  - Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate
VL  - 267
DO  - 10.1016/j.imlet.2024.106852
SP  - 106852
ER  - 

@article{
author = "Stegnjaić, Goran and Jevtić, Bojan and Lazarević, Milica and Ignjatović, Đurđica and Tomić, Mirko and Nikolovski, Neda and Bjelobaba, Ivana and Momčilović, Miljana and Dimitrijević, Mirjana and Miljković, Đorđe and Stanisavljević, Suzana",
year = "2024",
abstract = "We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund’s adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of T and B cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. T cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.",
publisher = "Elsevier",
journal = "Immunology Letters",
title = "Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate",
volume = "267",
doi = "10.1016/j.imlet.2024.106852",
pages = "106852"
}

Stegnjaić, G., Jevtić, B., Lazarević, M., Ignjatović, Đ., Tomić, M., Nikolovski, N., Bjelobaba, I., Momčilović, M., Dimitrijević, M., Miljković, Đ.,& Stanisavljević, S.. (2024). Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate. in Immunology Letters
Elsevier., 267, 106852.
https://doi.org/10.1016/j.imlet.2024.106852

Stegnjaić G, Jevtić B, Lazarević M, Ignjatović Đ, Tomić M, Nikolovski N, Bjelobaba I, Momčilović M, Dimitrijević M, Miljković Đ, Stanisavljević S. Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate. in Immunology Letters. 2024;267:106852.
doi:10.1016/j.imlet.2024.106852 .

Stegnjaić, Goran, Jevtić, Bojan, Lazarević, Milica, Ignjatović, Đurđica, Tomić, Mirko, Nikolovski, Neda, Bjelobaba, Ivana, Momčilović, Miljana, Dimitrijević, Mirjana, Miljković, Đorđe, Stanisavljević, Suzana, "Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate" in Immunology Letters, 267 (2024):106852,
https://doi.org/10.1016/j.imlet.2024.106852 . .

TY  - JOUR
AU  - Lazarević, Milica
AU  - Stegnjaić, Goran
AU  - Jevtić, Bojan
AU  - Despotović, Sanja
AU  - Ignjatović, Đurđica
AU  - Stanisavljević, Suzana
AU  - Nikolovski, Neda
AU  - Momčilović, Miljana
AU  - Fraser, Graeme L
AU  - Dimitrijević, Mirjana
AU  - Miljković, Đorđe
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6501
AB  - Experimental autoimmune encephalomyelitis (EAE) induced in inbred rodents, i.e., genetically identical animals kept under identical environmental conditions, shows variable clinical outcomes. We investigated such variations of EAE in Dark Agouti rats immunized with spinal cord homogenate and identified four groups: lethal, severe, moderate, and mild, at day 28 post immunization. Higher numbers of CD4+ T cells, helper T cells type 1 (Th1) and 17 (Th17) in particular, were detected in the spinal cord of the severe group in comparison with the moderate group. In addition, increased proportion of Th1 and Th17 cells, and heightened levels of interferon (IFN)-γ and interleukin (IL)-6 were detected in the small intestine lamina propria of the severe group. A selective agonist of free fatty acid receptor type 2 (Ffar2) applied orally in the inductive phase of EAE shifted the distribution of the disease outcomes towards milder forms. This effect was paralleled with potentiation of intestinal innate lymphoid cells type 3 (ILC3) regulatory properties, and diminished Th1 and Th17 cell response in the lymph nodes draining the site of immunization. Our results suggest that different clinical outcomes in DA rats are under determinative influence of intestinal ILC3 activity during the inductive phase of EAE.
PB  - Springer Nature
T2  - Journal of Neuroinflammation
T1  - Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity
VL  - 21
DO  - 10.1186/s12974-024-03017-7
SP  - 26
ER  - 

@article{
author = "Lazarević, Milica and Stegnjaić, Goran and Jevtić, Bojan and Despotović, Sanja and Ignjatović, Đurđica and Stanisavljević, Suzana and Nikolovski, Neda and Momčilović, Miljana and Fraser, Graeme L and Dimitrijević, Mirjana and Miljković, Đorđe",
year = "2024",
abstract = "Experimental autoimmune encephalomyelitis (EAE) induced in inbred rodents, i.e., genetically identical animals kept under identical environmental conditions, shows variable clinical outcomes. We investigated such variations of EAE in Dark Agouti rats immunized with spinal cord homogenate and identified four groups: lethal, severe, moderate, and mild, at day 28 post immunization. Higher numbers of CD4+ T cells, helper T cells type 1 (Th1) and 17 (Th17) in particular, were detected in the spinal cord of the severe group in comparison with the moderate group. In addition, increased proportion of Th1 and Th17 cells, and heightened levels of interferon (IFN)-γ and interleukin (IL)-6 were detected in the small intestine lamina propria of the severe group. A selective agonist of free fatty acid receptor type 2 (Ffar2) applied orally in the inductive phase of EAE shifted the distribution of the disease outcomes towards milder forms. This effect was paralleled with potentiation of intestinal innate lymphoid cells type 3 (ILC3) regulatory properties, and diminished Th1 and Th17 cell response in the lymph nodes draining the site of immunization. Our results suggest that different clinical outcomes in DA rats are under determinative influence of intestinal ILC3 activity during the inductive phase of EAE.",
publisher = "Springer Nature",
journal = "Journal of Neuroinflammation",
title = "Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity",
volume = "21",
doi = "10.1186/s12974-024-03017-7",
pages = "26"
}

Lazarević, M., Stegnjaić, G., Jevtić, B., Despotović, S., Ignjatović, Đ., Stanisavljević, S., Nikolovski, N., Momčilović, M., Fraser, G. L., Dimitrijević, M.,& Miljković, Đ.. (2024). Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity. in Journal of Neuroinflammation
Springer Nature., 21, 26.
https://doi.org/10.1186/s12974-024-03017-7

Lazarević M, Stegnjaić G, Jevtić B, Despotović S, Ignjatović Đ, Stanisavljević S, Nikolovski N, Momčilović M, Fraser GL, Dimitrijević M, Miljković Đ. Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity. in Journal of Neuroinflammation. 2024;21:26.
doi:10.1186/s12974-024-03017-7 .

Lazarević, Milica, Stegnjaić, Goran, Jevtić, Bojan, Despotović, Sanja, Ignjatović, Đurđica, Stanisavljević, Suzana, Nikolovski, Neda, Momčilović, Miljana, Fraser, Graeme L, Dimitrijević, Mirjana, Miljković, Đorđe, "Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity" in Journal of Neuroinflammation, 21 (2024):26,
https://doi.org/10.1186/s12974-024-03017-7 . .

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Mićić, Bojana
AU  - Tomić, Mirko
AU  - Đorđević, Ana
AU  - Macut, Đuro
AU  - Vojnović-Milutinović, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5821
AB  - Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increasein phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.
PB  - Amsterdam: Elsevier
T2  - Hormones and Behavior
T1  - Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome
VL  - 153
DO  - 10.1016/j.yhbeh.2023.105392
SP  - 105392
ER  - 

@article{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Mićić, Bojana and Tomić, Mirko and Đorđević, Ana and Macut, Đuro and Vojnović-Milutinović, Danijela",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increasein phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.",
publisher = "Amsterdam: Elsevier",
journal = "Hormones and Behavior",
title = "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome",
volume = "153",
doi = "10.1016/j.yhbeh.2023.105392",
pages = "105392"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Mićić, B., Tomić, M., Đorđević, A., Macut, Đ.,& Vojnović-Milutinović, D.. (2023). Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior
Amsterdam: Elsevier., 153, 105392.
https://doi.org/10.1016/j.yhbeh.2023.105392

Ignjatović Đ, Tovilović-Kovačević G, Mićić B, Tomić M, Đorđević A, Macut Đ, Vojnović-Milutinović D. Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior. 2023;153:105392.
doi:10.1016/j.yhbeh.2023.105392 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Mićić, Bojana, Tomić, Mirko, Đorđević, Ana, Macut, Đuro, Vojnović-Milutinović, Danijela, "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome" in Hormones and Behavior, 153 (2023):105392,
https://doi.org/10.1016/j.yhbeh.2023.105392 . .

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Mićić, Bojana
AU  - Tomić, Mirko
AU  - Đorđević, Ana
AU  - Macut, Đuro
AU  - Vojnović-Milutinović, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5820
AB  - Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increase
in phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.
PB  - Amsterdam: Elsevier
T2  - Hormones and Behavior
T1  - Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome
VL  - 153
DO  - 10.1016/j.yhbeh.2023.105392
SP  - 105392
ER  - 

@article{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Mićić, Bojana and Tomić, Mirko and Đorđević, Ana and Macut, Đuro and Vojnović-Milutinović, Danijela",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a complex disorder characterized by endocrine and metabolic abnormalities such as obesity and insulin resistance. PCOS is also associated with psychiatric disorders and cognitive impairment. The animal model of PCOS was induced by treating rats with 5α dihydrotestosterone (5α-DHT) and additionally modified to induce adiposity by litter size reduction (LSR). Spatial learning and memory were assessed using the Barnes Maze test, and striatal markers of synaptic plasticity were analyzed. Striatal insulin signaling was estimated by the levels of insulin receptor substrate 1 (IRS1), its inhibitory phosphorylation at Ser307, and glycogen synthase kinase-3α/β (GSK3α/β) activity. Both LSR and DHT treatment significantly decreased striatal protein levels of IRS1, followed by increased GSK3α/β activity in small litters. Results of the behavioral study showed that LSR had a negative effect on learning rate and memory retention, whereas DHT treatment did not induce impairment in memory formation. While protein levels of synaptophysin, GAP43, and postsynaptic density protein 95 (PSD-95) were not altered by the treatments, DHT treatment induced an increase
in phosphorylation of PSD-95 at Ser295 in both normal and small litters. This study revealed that LSR and DHT treatment suppressed insulin signaling by downregulating IRS1 in the striatum. However, DHT treatment did not have an adverse effect on learning and memory, probably due to compensatory elevation in pPSD-95-Ser295, which had a positive effect on synaptic strength. This implies that hyperandrogenemia in this setting does not represent a threat to spatial learning and memory, opposite to the effect of overnutrition-related adiposity.",
publisher = "Amsterdam: Elsevier",
journal = "Hormones and Behavior",
title = "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome",
volume = "153",
doi = "10.1016/j.yhbeh.2023.105392",
pages = "105392"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Mićić, B., Tomić, M., Đorđević, A., Macut, Đ.,& Vojnović-Milutinović, D.. (2023). Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior
Amsterdam: Elsevier., 153, 105392.
https://doi.org/10.1016/j.yhbeh.2023.105392

Ignjatović Đ, Tovilović-Kovačević G, Mićić B, Tomić M, Đorđević A, Macut Đ, Vojnović-Milutinović D. Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome. in Hormones and Behavior. 2023;153:105392.
doi:10.1016/j.yhbeh.2023.105392 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Mićić, Bojana, Tomić, Mirko, Đorđević, Ana, Macut, Đuro, Vojnović-Milutinović, Danijela, "Effects of early life overnutrition and hyperandrogenism on spatial learning and memory in a rat model of polycystic ovary syndrome" in Hormones and Behavior, 153 (2023):105392,
https://doi.org/10.1016/j.yhbeh.2023.105392 . .

TY  - CHAP
AU  - Ignjatović, Đurđica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5001
AB  - Gut microbiota of a child goes through very dynamic changes during first three years of life. In parallel, development of central nervous system occurs, making early childhood one of the most sensitive periods for both processes. Early life events which can disturb optimal microbiota development may also have detrimental effects on neurodevelopment due to bidirectional communication between microbiota and CNS in the microbiota-gut-brain axis. Factors like early life antibiotic, intestinal infections, perinatal stress, changes in diet or probiotic treatments may induce alterations in microbiota composition with long lasting effects on mental health.
PB  - Elsevier
T2  - Comprehensive Gut Microbiota
T1  - Early Life Events With Microbiota Mediated Effects on Brain Functions
VL  - 2
DO  - 10.1016/B978-0-12-819265-8.00058-9
SP  - 39
EP  - 58
ER  - 

@inbook{
editor = "Glibetic, Maria",
author = "Ignjatović, Đurđica",
year = "2022",
abstract = "Gut microbiota of a child goes through very dynamic changes during first three years of life. In parallel, development of central nervous system occurs, making early childhood one of the most sensitive periods for both processes. Early life events which can disturb optimal microbiota development may also have detrimental effects on neurodevelopment due to bidirectional communication between microbiota and CNS in the microbiota-gut-brain axis. Factors like early life antibiotic, intestinal infections, perinatal stress, changes in diet or probiotic treatments may induce alterations in microbiota composition with long lasting effects on mental health.",
publisher = "Elsevier",
journal = "Comprehensive Gut Microbiota",
booktitle = "Early Life Events With Microbiota Mediated Effects on Brain Functions",
volume = "2",
doi = "10.1016/B978-0-12-819265-8.00058-9",
pages = "39-58"
}

Glibetic, M.,& Ignjatović, Đ.. (2022). Early Life Events With Microbiota Mediated Effects on Brain Functions. in Comprehensive Gut Microbiota
Elsevier., 2, 39-58.
https://doi.org/10.1016/B978-0-12-819265-8.00058-9

Glibetic M, Ignjatović Đ. Early Life Events With Microbiota Mediated Effects on Brain Functions. in Comprehensive Gut Microbiota. 2022;2:39-58.
doi:10.1016/B978-0-12-819265-8.00058-9 .

Glibetic, Maria, Ignjatović, Đurđica, "Early Life Events With Microbiota Mediated Effects on Brain Functions" in Comprehensive Gut Microbiota, 2 (2022):39-58,
https://doi.org/10.1016/B978-0-12-819265-8.00058-9 . .

TY  - CONF
AU  - Mićić, Bojana
AU  - Radovanović, Marina
AU  - Tovilović-Kovačević, Gordana
AU  - Teofilović, Ana
AU  - Gligorovska, Ljupka
AU  - Vojnović-Milutinović, Danijela
AU  - Đorđević, Ana
AU  - Ignjatović, Đurđica
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4718
AB  - Fructose intake is associated with low-grade inflammation and increased oxidative
stress. Among long chain polyunsaturated fatty acids (LC-PUFAs), Ω-6 are recognized as a
contributing factor to inflammation, while Ω-3 LC-PUFAs are considered as functional foods with
beneficial effects, including inhibition of pro-inflammatory pathways. The aim of this study was to
analyze combined effects of physiologically relevant LC-PUFAs and fructose on inflammation and
antioxidant enzymes in the in vitro model of vascular endothelial cells. We examined the effects
of 0.5 mM fructose, alone and in combination with Ω-6 (arachidonic (AA) and linoleic (LA)) and
Ω-3 (eicosapentaenoic (EPA) and docosahexaenoic acid (DHA)) LC-PUFAs on expression of pro-
inflammatory cytokines (tumor necrosis factor α (TNFα) and interleukin 6 (IL6)) in EA.hy926 cells.
The protein levels of nuclear factor-κB (NF-κB) and IB, as well as its phosphorylation, together
with superoxide dismutase (SOD) 1 and 2, catalase and glutathione reductase (GR) were also
analyzed. Total ROS amounts were determined using flow cytometric analysis of cells stained
with redox sensitive dihydrorhodamine 123 dye. The results showed that treatment of cells with
fructose increased TNFα and decreased IL6 mRNA levels. Additional treatment with LA, DHA
and EPA reduced TNFα and led to further decrease of IL6 expression. The observed changes
were not associated with NFB activation. All examined enzymes were unchanged after fructose
treatment, while GR was increased by LC-PUFA addition. SOD2 was reduced in cells treated
with AA, LA and EPA, while increased ROS amounts were observed with AA, DHA and EPA. This
was also evident in combined treatment with fructose. These preliminary results suggest that
LC-PUFAs, besides effect on pro-inflammatory cytokines, reduce SOD2 levels and increase ROS.
The increased levels of ROS could stimulate production of PUFA-derived peroxides, which in
GSH-enriched environment might be converted into anti-inflammatory derivatives, additionally
suppressing inflammation in fructose treated endothelial cells
PB  - Elsevier Inc.
C3  - Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
T1  - Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells
DO  - 10.1016/j.freeradbiomed.2021.08.092
SP  - S79
ER  - 

@conference{
author = "Mićić, Bojana and Radovanović, Marina and Tovilović-Kovačević, Gordana and Teofilović, Ana and Gligorovska, Ljupka and Vojnović-Milutinović, Danijela and Đorđević, Ana and Ignjatović, Đurđica",
year = "2021",
abstract = "Fructose intake is associated with low-grade inflammation and increased oxidative
stress. Among long chain polyunsaturated fatty acids (LC-PUFAs), Ω-6 are recognized as a
contributing factor to inflammation, while Ω-3 LC-PUFAs are considered as functional foods with
beneficial effects, including inhibition of pro-inflammatory pathways. The aim of this study was to
analyze combined effects of physiologically relevant LC-PUFAs and fructose on inflammation and
antioxidant enzymes in the in vitro model of vascular endothelial cells. We examined the effects
of 0.5 mM fructose, alone and in combination with Ω-6 (arachidonic (AA) and linoleic (LA)) and
Ω-3 (eicosapentaenoic (EPA) and docosahexaenoic acid (DHA)) LC-PUFAs on expression of pro-
inflammatory cytokines (tumor necrosis factor α (TNFα) and interleukin 6 (IL6)) in EA.hy926 cells.
The protein levels of nuclear factor-κB (NF-κB) and IB, as well as its phosphorylation, together
with superoxide dismutase (SOD) 1 and 2, catalase and glutathione reductase (GR) were also
analyzed. Total ROS amounts were determined using flow cytometric analysis of cells stained
with redox sensitive dihydrorhodamine 123 dye. The results showed that treatment of cells with
fructose increased TNFα and decreased IL6 mRNA levels. Additional treatment with LA, DHA
and EPA reduced TNFα and led to further decrease of IL6 expression. The observed changes
were not associated with NFB activation. All examined enzymes were unchanged after fructose
treatment, while GR was increased by LC-PUFA addition. SOD2 was reduced in cells treated
with AA, LA and EPA, while increased ROS amounts were observed with AA, DHA and EPA. This
was also evident in combined treatment with fructose. These preliminary results suggest that
LC-PUFAs, besides effect on pro-inflammatory cytokines, reduce SOD2 levels and increase ROS.
The increased levels of ROS could stimulate production of PUFA-derived peroxides, which in
GSH-enriched environment might be converted into anti-inflammatory derivatives, additionally
suppressing inflammation in fructose treated endothelial cells",
publisher = "Elsevier Inc.",
journal = "Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia",
title = "Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells",
doi = "10.1016/j.freeradbiomed.2021.08.092",
pages = "S79"
}

Mićić, B., Radovanović, M., Tovilović-Kovačević, G., Teofilović, A., Gligorovska, L., Vojnović-Milutinović, D., Đorđević, A.,& Ignjatović, Đ.. (2021). Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
Elsevier Inc.., S79.
https://doi.org/10.1016/j.freeradbiomed.2021.08.092

Mićić B, Radovanović M, Tovilović-Kovačević G, Teofilović A, Gligorovska L, Vojnović-Milutinović D, Đorđević A, Ignjatović Đ. Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia. 2021;:S79.
doi:10.1016/j.freeradbiomed.2021.08.092 .

Mićić, Bojana, Radovanović, Marina, Tovilović-Kovačević, Gordana, Teofilović, Ana, Gligorovska, Ljupka, Vojnović-Milutinović, Danijela, Đorđević, Ana, Ignjatović, Đurđica, "Potentiation of polyunsaturated fatty acids anti-inflammatory action through redox signaling in fructose-treated endothelial cells" in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia (2021):S79,
https://doi.org/10.1016/j.freeradbiomed.2021.08.092 . .

TY  - JOUR
AU  - Vratarić, Miloš
AU  - Šenk, Vladimir
AU  - Bursać, Biljana
AU  - Gligorovska, Ljupka
AU  - Ignjatović, Đurđica
AU  - Kovačević, Sanja
AU  - Veličković, Nataša
AU  - Đorđević, Ana
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/10.1002/biof.1802
UR  - http://www.ncbi.nlm.nih.gov/pubmed/34767656
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4687
AB  - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that represents a link between diet-induced inflammation and insulin resistance. Our aim was to examine whether fructose diet affects inflammation and insulin signaling in the prefrontal cortex (PFC) of Mif knockout mice (MIF-KO), and their possible link to neural plasticity and behavior. We analyzed nuclear factor κB (NF-κB) and glucocorticoid signaling, expression of F4/80, IL-1β, TNF-α, TLR-4, MyD88, arginase 1 (Arg-1), mannose receptor (Mrc-1), and leukemia inhibitory factor (Lif) to assess inflammation in the PFC of C57/BL6J and MIF-KO mice consuming 20% fructose solution for 9 weeks. Insulin receptor (IR), IRS-1 serine phosphorylations (307 and 1101) and activity of PKCα, Akt, GSK-3β and AMPKα were used to analyze insulin signaling. Brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1) mRNA levels, together with synapthophysin and PSD-95 protein level and calcium calmodulin-dependent kinase 2 (CaMKII) activity, were used as plasticity markers. Behavior was examined in elevated plus maze, light dark box and novel object recognition test. The results showed concomitant increase of Tnf-α, Tlr-4, MyD88 and M2 microglia markers (Arg-1, Mrc-1, Lif) in the PFC of MIF-KO, paralleled with unchanged glucocorticoid and insulin signaling. Increase of BDNF and IGF-1 was paralleled with increased CaMKII activity, decreased PSD-95 protein level, anxiogenic behavior, and impaired memory in MIF-KO mice. Fructose feeding restored these parameters in the PFC to the control level and mitigated behavioral changes, suggesting that ameliorating effects of fructose on neuroinflammation and behavior depend on the presence of MIF.
T2  - BioFactors (Oxford, England)
T1  - Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.
DO  - 10.1002/biof.1802
ER  - 

@article{
author = "Vratarić, Miloš and Šenk, Vladimir and Bursać, Biljana and Gligorovska, Ljupka and Ignjatović, Đurđica and Kovačević, Sanja and Veličković, Nataša and Đorđević, Ana",
year = "2021",
abstract = "Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that represents a link between diet-induced inflammation and insulin resistance. Our aim was to examine whether fructose diet affects inflammation and insulin signaling in the prefrontal cortex (PFC) of Mif knockout mice (MIF-KO), and their possible link to neural plasticity and behavior. We analyzed nuclear factor κB (NF-κB) and glucocorticoid signaling, expression of F4/80, IL-1β, TNF-α, TLR-4, MyD88, arginase 1 (Arg-1), mannose receptor (Mrc-1), and leukemia inhibitory factor (Lif) to assess inflammation in the PFC of C57/BL6J and MIF-KO mice consuming 20% fructose solution for 9 weeks. Insulin receptor (IR), IRS-1 serine phosphorylations (307 and 1101) and activity of PKCα, Akt, GSK-3β and AMPKα were used to analyze insulin signaling. Brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1) mRNA levels, together with synapthophysin and PSD-95 protein level and calcium calmodulin-dependent kinase 2 (CaMKII) activity, were used as plasticity markers. Behavior was examined in elevated plus maze, light dark box and novel object recognition test. The results showed concomitant increase of Tnf-α, Tlr-4, MyD88 and M2 microglia markers (Arg-1, Mrc-1, Lif) in the PFC of MIF-KO, paralleled with unchanged glucocorticoid and insulin signaling. Increase of BDNF and IGF-1 was paralleled with increased CaMKII activity, decreased PSD-95 protein level, anxiogenic behavior, and impaired memory in MIF-KO mice. Fructose feeding restored these parameters in the PFC to the control level and mitigated behavioral changes, suggesting that ameliorating effects of fructose on neuroinflammation and behavior depend on the presence of MIF.",
journal = "BioFactors (Oxford, England)",
title = "Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.",
doi = "10.1002/biof.1802"
}

Vratarić, M., Šenk, V., Bursać, B., Gligorovska, L., Ignjatović, Đ., Kovačević, S., Veličković, N.,& Đorđević, A.. (2021). Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.. in BioFactors (Oxford, England).
https://doi.org/10.1002/biof.1802

Vratarić M, Šenk V, Bursać B, Gligorovska L, Ignjatović Đ, Kovačević S, Veličković N, Đorđević A. Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.. in BioFactors (Oxford, England). 2021;.
doi:10.1002/biof.1802 .

Vratarić, Miloš, Šenk, Vladimir, Bursać, Biljana, Gligorovska, Ljupka, Ignjatović, Đurđica, Kovačević, Sanja, Veličković, Nataša, Đorđević, Ana, "Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice." in BioFactors (Oxford, England) (2021),
https://doi.org/10.1002/biof.1802 . .

TY  - JOUR
AU  - Vratarić, Miloš
AU  - Šenk, Vladimir
AU  - Bursać, Biljana
AU  - Gligorovska, Ljupka
AU  - Ignjatović, Đurđica
AU  - Kovačević, Sanja
AU  - Veličković, Nataša
AU  - Đorđević, Ana
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/10.1002/biof.1802
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4773
AB  - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that represents a link between diet-induced inflammation and insulin resistance. Our aim was to examine whether fructose diet affects inflammation and insulin signaling in the prefrontal cortex (PFC) of Mif knockout mice (MIF-KO), and their possible link to neural plasticity and behavior. We analyzed nuclear factor κB (NF-κB) and glucocorticoid signaling, expression of F4/80, IL-1β, TNF-α, TLR-4, MyD88, arginase 1 (Arg-1), mannose receptor (Mrc-1), and leukemia inhibitory factor (Lif) to assess inflammation in the PFC of C57/BL6J and MIF-KO mice consuming 20% fructose solution for 9 weeks. Insulin receptor (IR), IRS-1 serine phosphorylations (307 and 1101) and activity of PKCα, Akt, GSK-3β and AMPKα were used to analyze insulin signaling. Brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1) mRNA levels, together with synapthophysin and PSD-95 protein level and calcium calmodulin-dependent kinase 2 (CaMKII) activity, were used as plasticity markers. Behavior was examined in elevated plus maze, light dark box and novel object recognition test. The results showed concomitant increase of Tnf-α, Tlr-4, MyD88 and M2 microglia markers (Arg-1, Mrc-1, Lif) in the PFC of MIF-KO, paralleled with unchanged glucocorticoid and insulin signaling. Increase of BDNF and IGF-1 was paralleled with increased CaMKII activity, decreased PSD-95 protein level, anxiogenic behavior, and impaired memory in MIF-KO mice. Fructose feeding restored these parameters in the PFC to the control level and mitigated behavioral changes, suggesting that ameliorating effects of fructose on neuroinflammation and behavior depend on the presence of MIF.
T2  - BioFactors (Oxford, England)
T1  - Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.
DO  - 10.1002/biof.1802
ER  - 

@article{
author = "Vratarić, Miloš and Šenk, Vladimir and Bursać, Biljana and Gligorovska, Ljupka and Ignjatović, Đurđica and Kovačević, Sanja and Veličković, Nataša and Đorđević, Ana",
year = "2021",
abstract = "Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that represents a link between diet-induced inflammation and insulin resistance. Our aim was to examine whether fructose diet affects inflammation and insulin signaling in the prefrontal cortex (PFC) of Mif knockout mice (MIF-KO), and their possible link to neural plasticity and behavior. We analyzed nuclear factor κB (NF-κB) and glucocorticoid signaling, expression of F4/80, IL-1β, TNF-α, TLR-4, MyD88, arginase 1 (Arg-1), mannose receptor (Mrc-1), and leukemia inhibitory factor (Lif) to assess inflammation in the PFC of C57/BL6J and MIF-KO mice consuming 20% fructose solution for 9 weeks. Insulin receptor (IR), IRS-1 serine phosphorylations (307 and 1101) and activity of PKCα, Akt, GSK-3β and AMPKα were used to analyze insulin signaling. Brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1) mRNA levels, together with synapthophysin and PSD-95 protein level and calcium calmodulin-dependent kinase 2 (CaMKII) activity, were used as plasticity markers. Behavior was examined in elevated plus maze, light dark box and novel object recognition test. The results showed concomitant increase of Tnf-α, Tlr-4, MyD88 and M2 microglia markers (Arg-1, Mrc-1, Lif) in the PFC of MIF-KO, paralleled with unchanged glucocorticoid and insulin signaling. Increase of BDNF and IGF-1 was paralleled with increased CaMKII activity, decreased PSD-95 protein level, anxiogenic behavior, and impaired memory in MIF-KO mice. Fructose feeding restored these parameters in the PFC to the control level and mitigated behavioral changes, suggesting that ameliorating effects of fructose on neuroinflammation and behavior depend on the presence of MIF.",
journal = "BioFactors (Oxford, England)",
title = "Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.",
doi = "10.1002/biof.1802"
}

Vratarić, M., Šenk, V., Bursać, B., Gligorovska, L., Ignjatović, Đ., Kovačević, S., Veličković, N.,& Đorđević, A.. (2021). Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.. in BioFactors (Oxford, England).
https://doi.org/10.1002/biof.1802

Vratarić M, Šenk V, Bursać B, Gligorovska L, Ignjatović Đ, Kovačević S, Veličković N, Đorđević A. Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.. in BioFactors (Oxford, England). 2021;.
doi:10.1002/biof.1802 .

Vratarić, Miloš, Šenk, Vladimir, Bursać, Biljana, Gligorovska, Ljupka, Ignjatović, Đurđica, Kovačević, Sanja, Veličković, Nataša, Đorđević, Ana, "Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice." in BioFactors (Oxford, England) (2021),
https://doi.org/10.1002/biof.1802 . .

TY  - JOUR
AU  - Bojović, Katarina
AU  - Ignjatović, Đurđica
AU  - Soković Bajić, Svetlana
AU  - Vojnović-Milutinović, Danijela
AU  - Tomić, Mirko
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32509596
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7248180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3698
AB  - While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to Clostridium species, in the NDD patient group compared to the Control group: Desulfotomaculum guttoideum (P < 0.01), Intestinibacter bartlettii (P < 0.05), and Romboutsia ilealis (P < 0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. Enterococcus faecalis (P < 0.05), Enterococcus gallinarum (P < 0.01), Streptococcus pasteurianus (P < 0.05), Lactobacillus rhamnosus (P < 0.01) and Bifidobacteria sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria Faecalibacterium prausnitzii (P < 0.01), Butyricicoccus pullicaecorum (P < 0.05), and Eubacterium rectale (P = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.
PB  - Frontiers Media S.A.
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.
VL  - 10
DO  - 10.3389/fcimb.2020.00223
SP  - 223
ER  - 

@article{
author = "Bojović, Katarina and Ignjatović, Đurđica and Soković Bajić, Svetlana and Vojnović-Milutinović, Danijela and Tomić, Mirko and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to Clostridium species, in the NDD patient group compared to the Control group: Desulfotomaculum guttoideum (P < 0.01), Intestinibacter bartlettii (P < 0.05), and Romboutsia ilealis (P < 0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. Enterococcus faecalis (P < 0.05), Enterococcus gallinarum (P < 0.01), Streptococcus pasteurianus (P < 0.05), Lactobacillus rhamnosus (P < 0.01) and Bifidobacteria sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria Faecalibacterium prausnitzii (P < 0.01), Butyricicoccus pullicaecorum (P < 0.05), and Eubacterium rectale (P = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.",
volume = "10",
doi = "10.3389/fcimb.2020.00223",
pages = "223"
}

Bojović, K., Ignjatović, Đ., Soković Bajić, S., Vojnović-Milutinović, D., Tomić, M., Golić, N.,& Tolinački, M.. (2020). Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.. in Frontiers in Cellular and Infection Microbiology
Frontiers Media S.A.., 10, 223.
https://doi.org/10.3389/fcimb.2020.00223

Bojović K, Ignjatović Đ, Soković Bajić S, Vojnović-Milutinović D, Tomić M, Golić N, Tolinački M. Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.. in Frontiers in Cellular and Infection Microbiology. 2020;10:223.
doi:10.3389/fcimb.2020.00223 .

Bojović, Katarina, Ignjatović, Đurđica, Soković Bajić, Svetlana, Vojnović-Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja, "Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders." in Frontiers in Cellular and Infection Microbiology, 10 (2020):223,
https://doi.org/10.3389/fcimb.2020.00223 . .

TY  - CONF
AU  - Gligorovska, Ljupka
AU  - Ljumović, Kristina
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Đorđević, Ana
PY  - 2020
UR  - https://sites.google.com/view/costmeetingbelgrade/home
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3992
AB  - Modern lifestyle, characterized by increased consumption of fructose-enriched beverages, can lead to obesity, type 2 diabetes and cardiovascular diseases. Since increased fructose intake is often associated with chronic low-grade inflammation and increased oxidative stress in various tissues, the aim was to investigate the level of inflammation and antioxidant protection in endothelial, neuroblastoma and preadipocyte cell lines treated with fructose. We examined the effects of 4-hour treatment with different concentrations of fructose (0.5 mM, 2.5 mM and 10 mM) on gene expression of pro-inflammatory cytokines (tumor necrosis factor α (TNFα), interleukin (IL) 1β and 6) in endothelial (EA.hy926), neuroblastoma (SH-SY5Y) and differentiated preadipocyte (3T3-F442A) cells. The protein levels of nuclear factor-κB (NF-κB), IκB, superoxide dismutase (SOD) 1 and 2, catalase, glutathione reductase and glutathione S-transferase, were also analyzed. In endothelial cells, 0.5 mM and 2.5 mM fructose treatment caused significant increase of TNFα mRNA level, while in SH-SY5Y and differentiated 3T3-F442A cells, IL-6 mRNA level was elevated after 0.5 mM fructose treatment. Although fructose increased pro-inflammatory cytokines in cell-type and dose-specific manner, decreased IκB protein level was detected only in adipocytes regardless of the dose. Finally, among all examined antioxidant enzymes, only SOD2 was significantly reduced in all cell types upon 2.5 mM fructose treatment. These preliminary results show that the effects of fructose on inflammation and antioxidant enzymes are both cell-type and dose specific. A marked decrease in the levels of SOD2, observed in all examined cells, can be associated with lower antioxidative defense under moderate fructose concentration.
PB  - University of Belgrade: Faculty of Pharmacy
C3  - Nutraceuticals in balancing redox status in ageing and age-related diseases, Book of Abstracts
T1  - Inflammatory and antioxidative response of different cell lines after in vitro fructose treatment
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3992
ER  - 

@conference{
author = "Gligorovska, Ljupka and Ljumović, Kristina and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Đorđević, Ana",
year = "2020",
abstract = "Modern lifestyle, characterized by increased consumption of fructose-enriched beverages, can lead to obesity, type 2 diabetes and cardiovascular diseases. Since increased fructose intake is often associated with chronic low-grade inflammation and increased oxidative stress in various tissues, the aim was to investigate the level of inflammation and antioxidant protection in endothelial, neuroblastoma and preadipocyte cell lines treated with fructose. We examined the effects of 4-hour treatment with different concentrations of fructose (0.5 mM, 2.5 mM and 10 mM) on gene expression of pro-inflammatory cytokines (tumor necrosis factor α (TNFα), interleukin (IL) 1β and 6) in endothelial (EA.hy926), neuroblastoma (SH-SY5Y) and differentiated preadipocyte (3T3-F442A) cells. The protein levels of nuclear factor-κB (NF-κB), IκB, superoxide dismutase (SOD) 1 and 2, catalase, glutathione reductase and glutathione S-transferase, were also analyzed. In endothelial cells, 0.5 mM and 2.5 mM fructose treatment caused significant increase of TNFα mRNA level, while in SH-SY5Y and differentiated 3T3-F442A cells, IL-6 mRNA level was elevated after 0.5 mM fructose treatment. Although fructose increased pro-inflammatory cytokines in cell-type and dose-specific manner, decreased IκB protein level was detected only in adipocytes regardless of the dose. Finally, among all examined antioxidant enzymes, only SOD2 was significantly reduced in all cell types upon 2.5 mM fructose treatment. These preliminary results show that the effects of fructose on inflammation and antioxidant enzymes are both cell-type and dose specific. A marked decrease in the levels of SOD2, observed in all examined cells, can be associated with lower antioxidative defense under moderate fructose concentration.",
publisher = "University of Belgrade: Faculty of Pharmacy",
journal = "Nutraceuticals in balancing redox status in ageing and age-related diseases, Book of Abstracts",
title = "Inflammatory and antioxidative response of different cell lines after in vitro fructose treatment",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3992"
}

Gligorovska, L., Ljumović, K., Ignjatović, Đ., Tovilović-Kovačević, G.,& Đorđević, A.. (2020). Inflammatory and antioxidative response of different cell lines after in vitro fructose treatment. in Nutraceuticals in balancing redox status in ageing and age-related diseases, Book of Abstracts
University of Belgrade: Faculty of Pharmacy., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_3992

Gligorovska L, Ljumović K, Ignjatović Đ, Tovilović-Kovačević G, Đorđević A. Inflammatory and antioxidative response of different cell lines after in vitro fructose treatment. in Nutraceuticals in balancing redox status in ageing and age-related diseases, Book of Abstracts. 2020;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_3992 .

Gligorovska, Ljupka, Ljumović, Kristina, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Đorđević, Ana, "Inflammatory and antioxidative response of different cell lines after in vitro fructose treatment" in Nutraceuticals in balancing redox status in ageing and age-related diseases, Book of Abstracts (2020):36,
https://hdl.handle.net/21.15107/rcub_ibiss_3992 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Soković-Bajić, Svetlana
AU  - Bojović, Katarina
AU  - Vojnović-Milutinović, Danijela
AU  - Tomić, Mirko
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5431
AB  - Objective: Clinical reports indicated increased incidence of gastrointestinal (GI) disturbances and dysbiosis in children with neurodevelopmental disorders (NDD) as well as acceleration of the recovery after the dysbiosis treatment. The aim of this study was to evaluate gut microbiota diversity and to identify bacterial strains which incidences were different between patients’ and control group. This is the first study evaluating gut microbiota diversity and composition in children with NDD.
Conclusions: Our study reveals that the intestinal microbiota from children with NDDs differs from the microbiota of healthy children. Similarity of microbiota composition in patient groups with similar neurological and behavioral symptoms indicate that intestinal microbiota might have role in pathophysiology of NDD. Supplementation with several health promoting strains, could be safe adjuvant therapy in treatment of NDD accompanied with GI disturbances.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders
VL  - 54
DO  - 10.1097/MCG.0000000000001292
ER  - 

@conference{
author = "Ignjatović, Đurđica and Soković-Bajić, Svetlana and Bojović, Katarina and Vojnović-Milutinović, Danijela and Tomić, Mirko and Golić, Nataša and Tolinački, Maja",
year = "2019",
abstract = "Objective: Clinical reports indicated increased incidence of gastrointestinal (GI) disturbances and dysbiosis in children with neurodevelopmental disorders (NDD) as well as acceleration of the recovery after the dysbiosis treatment. The aim of this study was to evaluate gut microbiota diversity and to identify bacterial strains which incidences were different between patients’ and control group. This is the first study evaluating gut microbiota diversity and composition in children with NDD.
Conclusions: Our study reveals that the intestinal microbiota from children with NDDs differs from the microbiota of healthy children. Similarity of microbiota composition in patient groups with similar neurological and behavioral symptoms indicate that intestinal microbiota might have role in pathophysiology of NDD. Supplementation with several health promoting strains, could be safe adjuvant therapy in treatment of NDD accompanied with GI disturbances.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders",
volume = "54",
doi = "10.1097/MCG.0000000000001292"
}

Ignjatović, Đ., Soković-Bajić, S., Bojović, K., Vojnović-Milutinović, D., Tomić, M., Golić, N.,& Tolinački, M.. (2019). Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 54.
https://doi.org/10.1097/MCG.0000000000001292

Ignjatović Đ, Soković-Bajić S, Bojović K, Vojnović-Milutinović D, Tomić M, Golić N, Tolinački M. Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;54.
doi:10.1097/MCG.0000000000001292 .

Ignjatović, Đurđica, Soković-Bajić, Svetlana, Bojović, Katarina, Vojnović-Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja, "Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy, 54 (2019),
https://doi.org/10.1097/MCG.0000000000001292 . .

TY  - JOUR
AU  - Bojović, Katarina
AU  - Stanković, Biljana
AU  - Kotur, Nikola
AU  - Krstić Milošević, Dijana
AU  - Gašić, Vladimir
AU  - Pavlović, Sonja
AU  - Zukić, Branka
AU  - Ignjatović, Đurđica
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/1028415X.2017.1352121
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2823
AB  - Gastrointestinal disturbances, nutritional deficiencies, and food intolerances are frequently observed in children with neurodevelopmental disorders (NDD). To reveal possible association of celiac disease risk variants (HLA-DQ), lactose intolerance associated variant (LCT-13910C > T) as well as variant associated with vitamin D function (VDR FokI) with NDD, polymerase chain reaction-based methodology was used. Additionally, intestinal peptide permeability was estimated in NDD patients and healthy children by measuring the level of peptides in urine using high-performance liquid chromatography. Levels of opioid peptides, casomorphin 8, and gluten exorphin C were significantly elevated in urine samples of NDD patients (P = 0.004 and P = 0.005, respectively), but no association of genetic risk variants for celiac disease and lactose intolerance with NDD was found. Our results indicate that increased intestinal peptide permeability observed in analyzed NDD patients is not associated with genetic predictors of celiac disease or lactose intolerance. We have also found that FF genotype of VDR FokI and lower serum levels of vitamin D (25-OH) showed association with childhood autism (CHA), a subgroup of NDD. We hypothesize that vitamin D might be important for the development of CHA.
T2  - Nutritional Neuroscience
T1  - Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders
IS  - 1
VL  - 22
DO  - 10.1080/1028415X.2017.1352121
SP  - 40
EP  - 50
ER  - 

@article{
author = "Bojović, Katarina and Stanković, Biljana and Kotur, Nikola and Krstić Milošević, Dijana and Gašić, Vladimir and Pavlović, Sonja and Zukić, Branka and Ignjatović, Đurđica",
year = "2019",
abstract = "Gastrointestinal disturbances, nutritional deficiencies, and food intolerances are frequently observed in children with neurodevelopmental disorders (NDD). To reveal possible association of celiac disease risk variants (HLA-DQ), lactose intolerance associated variant (LCT-13910C > T) as well as variant associated with vitamin D function (VDR FokI) with NDD, polymerase chain reaction-based methodology was used. Additionally, intestinal peptide permeability was estimated in NDD patients and healthy children by measuring the level of peptides in urine using high-performance liquid chromatography. Levels of opioid peptides, casomorphin 8, and gluten exorphin C were significantly elevated in urine samples of NDD patients (P = 0.004 and P = 0.005, respectively), but no association of genetic risk variants for celiac disease and lactose intolerance with NDD was found. Our results indicate that increased intestinal peptide permeability observed in analyzed NDD patients is not associated with genetic predictors of celiac disease or lactose intolerance. We have also found that FF genotype of VDR FokI and lower serum levels of vitamin D (25-OH) showed association with childhood autism (CHA), a subgroup of NDD. We hypothesize that vitamin D might be important for the development of CHA.",
journal = "Nutritional Neuroscience",
title = "Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders",
number = "1",
volume = "22",
doi = "10.1080/1028415X.2017.1352121",
pages = "40-50"
}

Bojović, K., Stanković, B., Kotur, N., Krstić Milošević, D., Gašić, V., Pavlović, S., Zukić, B.,& Ignjatović, Đ.. (2019). Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders. in Nutritional Neuroscience, 22(1), 40-50.
https://doi.org/10.1080/1028415X.2017.1352121

Bojović K, Stanković B, Kotur N, Krstić Milošević D, Gašić V, Pavlović S, Zukić B, Ignjatović Đ. Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders. in Nutritional Neuroscience. 2019;22(1):40-50.
doi:10.1080/1028415X.2017.1352121 .

Bojović, Katarina, Stanković, Biljana, Kotur, Nikola, Krstić Milošević, Dijana, Gašić, Vladimir, Pavlović, Sonja, Zukić, Branka, Ignjatović, Đurđica, "Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders" in Nutritional Neuroscience, 22, no. 1 (2019):40-50,
https://doi.org/10.1080/1028415X.2017.1352121 . .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Đorđević, Ana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5649
AB  - Dramatičan porast prevalence gojaznosti u populaciji dece i adolescenata predstavlja vodeći zdravstveni problem savremenog društva. Prema dosadašnjim istraživanjima, glavni oblik dijetetskog lečenja gojaznosti kod dece i adolescenata predstavlja uravnotežena hipokalorijska ishrana koju karakteriše povećan unos hrane koja sadrži vlakna, pre svega voće, povrće i cela zrna žitarica. Kako se sastav crevne mikrobiote menja u zavisnosti od režima ishrane, brojne studije su pokazale postojanje veze između sastava mikrobiote u fecesu gojaznih i njihove telesne težine. Imajući to na umu nameće se pitanje da li je smanjenje gojaznosti nakon hipokalorijske ishrane posledica promena u količini i sastavu crevne mikrobiote i produkata njene aktivnosti kao što su masne kiseline kratkog lanca (eng. Short chain fatty acid, SCFA). Masne kiseline kratkog lanca, pre svega sirćetna, propionska i buterna, nastaju procesom fermentacije nerastvorljivih biljnih vlakana u debelom crevu od strane crevne mikrobiote, a pored uloge u održavanja crevne homeostaze svoju biološku funkciju ostvaruju i kao signalni molekuli koji modulišu metabolizam lipida u masnom tkivu. Interesantno je da u zavisnosti od tipa i zastupljenosti, masne kiseline kratkog lanca mogu da stimulišu kako proces lipolize, tako i procese adipogeneze i lipogeneze. U procesu regulacije lipolize glavnu ulogu ima enzim HSL (eng. hormone sensitive lipase), dok su ključni regulatori procesa adipo- i lipogeneze u masnom tkivu transkripcioni regulatori PPARγ (eng. peroxisome proliferator-activated receptor γ), SREBP-1 (eng. sterol regulatory element binding protein-1) i ChREBP (eng. carbohydrate response element binding protein). Rasvetljavanje molekularnih mehanizama delovanja masnih kiselina kratkog lanca na ćelije masnog tkiva, pre svega na procese regulacije adipogeneze i lipidnog metabolizma može doprineti uspešnijoj prevenciji i lečenju gojaznosti putem promena u sastavu crevne mikrobiote i njenih produkata koje se mogu kontrolisati različitim režimima ishrane.
AB  - The dramatic increase in the prevalence of obesity in the population of children and adolescents represents the leading health problem of modern society. According to previous research, the main form of dietary treatment of obesity in children and adolescents is a balanced hypocaloric diet characterized by an increased intake of food containing fiber, primarily fruits, vegetables and whole grains. As the composition of the intestinal microbiota changes depending on the diet, numerous studies have shown the existence of a relationship between the composition of the microbiota in the feces of obese people and their body weight. Bearing this in mind, the question arises whether the reduction of obesity after a hypocaloric diet is a consequence of changes in the amount and composition of the intestinal microbiota and the products of its activity, such as short chain fatty acids (SCFA). Short-chain fatty acids, primarily acetic, propionic and butyric, are produced by the process of fermentation of insoluble plant fibers in the large intestine by intestinal microbiota, and in addition to their role in maintaining intestinal homeostasis, they also perform their biological function as signaling molecules that modulate lipid metabolism in adipose tissue. It is interesting that, depending on the type and abundance, short-chain fatty acids can stimulate the process of lipolysis as well as the processes of adipogenesis and lipogenesis. In the process of regulating lipolysis, the main role is played by the enzyme HSL (hormone sensitive lipase), while the key regulators of the process of adipo- and lipogenesis in adipose tissue are the transcriptional regulators PPARγ (eng. peroxisome proliferator-activated receptor γ), SREBP-1 (eng. sterol regulatory element binding protein-1) and ChREBP (eng. carbohydrate response element binding protein). Elucidation of the molecular mechanisms of action of short-chain fatty acids on adipose tissue cells, primarily on the processes of regulation of adipogenesis and lipid metabolism, can contribute to more successful prevention and treatment of obesity through changes in the composition of intestinal microbiota and its products that can be controlled by different dietary regimes.
PB  - Niš : Educo Events
C3  - Zbornik radova: 1. Srpski kongres dečje endokrinologije "Čigota 2018" sa međunarodnim učešćem; 2018 Apr 26-28; Zlatibor, Serbia
T1  - The role of gut microbiota in the development of obesity: molecular mechanisms and therapeutic implications
T1  - Uloga crevne mikrobiote u razvoju gojaznosti: molekularni mehanizmi i terapijske implikacije
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5649
ER  - 

@conference{
author = "Ignjatović, Đurđica and Đorđević, Ana",
year = "2018",
abstract = "Dramatičan porast prevalence gojaznosti u populaciji dece i adolescenata predstavlja vodeći zdravstveni problem savremenog društva. Prema dosadašnjim istraživanjima, glavni oblik dijetetskog lečenja gojaznosti kod dece i adolescenata predstavlja uravnotežena hipokalorijska ishrana koju karakteriše povećan unos hrane koja sadrži vlakna, pre svega voće, povrće i cela zrna žitarica. Kako se sastav crevne mikrobiote menja u zavisnosti od režima ishrane, brojne studije su pokazale postojanje veze između sastava mikrobiote u fecesu gojaznih i njihove telesne težine. Imajući to na umu nameće se pitanje da li je smanjenje gojaznosti nakon hipokalorijske ishrane posledica promena u količini i sastavu crevne mikrobiote i produkata njene aktivnosti kao što su masne kiseline kratkog lanca (eng. Short chain fatty acid, SCFA). Masne kiseline kratkog lanca, pre svega sirćetna, propionska i buterna, nastaju procesom fermentacije nerastvorljivih biljnih vlakana u debelom crevu od strane crevne mikrobiote, a pored uloge u održavanja crevne homeostaze svoju biološku funkciju ostvaruju i kao signalni molekuli koji modulišu metabolizam lipida u masnom tkivu. Interesantno je da u zavisnosti od tipa i zastupljenosti, masne kiseline kratkog lanca mogu da stimulišu kako proces lipolize, tako i procese adipogeneze i lipogeneze. U procesu regulacije lipolize glavnu ulogu ima enzim HSL (eng. hormone sensitive lipase), dok su ključni regulatori procesa adipo- i lipogeneze u masnom tkivu transkripcioni regulatori PPARγ (eng. peroxisome proliferator-activated receptor γ), SREBP-1 (eng. sterol regulatory element binding protein-1) i ChREBP (eng. carbohydrate response element binding protein). Rasvetljavanje molekularnih mehanizama delovanja masnih kiselina kratkog lanca na ćelije masnog tkiva, pre svega na procese regulacije adipogeneze i lipidnog metabolizma može doprineti uspešnijoj prevenciji i lečenju gojaznosti putem promena u sastavu crevne mikrobiote i njenih produkata koje se mogu kontrolisati različitim režimima ishrane., The dramatic increase in the prevalence of obesity in the population of children and adolescents represents the leading health problem of modern society. According to previous research, the main form of dietary treatment of obesity in children and adolescents is a balanced hypocaloric diet characterized by an increased intake of food containing fiber, primarily fruits, vegetables and whole grains. As the composition of the intestinal microbiota changes depending on the diet, numerous studies have shown the existence of a relationship between the composition of the microbiota in the feces of obese people and their body weight. Bearing this in mind, the question arises whether the reduction of obesity after a hypocaloric diet is a consequence of changes in the amount and composition of the intestinal microbiota and the products of its activity, such as short chain fatty acids (SCFA). Short-chain fatty acids, primarily acetic, propionic and butyric, are produced by the process of fermentation of insoluble plant fibers in the large intestine by intestinal microbiota, and in addition to their role in maintaining intestinal homeostasis, they also perform their biological function as signaling molecules that modulate lipid metabolism in adipose tissue. It is interesting that, depending on the type and abundance, short-chain fatty acids can stimulate the process of lipolysis as well as the processes of adipogenesis and lipogenesis. In the process of regulating lipolysis, the main role is played by the enzyme HSL (hormone sensitive lipase), while the key regulators of the process of adipo- and lipogenesis in adipose tissue are the transcriptional regulators PPARγ (eng. peroxisome proliferator-activated receptor γ), SREBP-1 (eng. sterol regulatory element binding protein-1) and ChREBP (eng. carbohydrate response element binding protein). Elucidation of the molecular mechanisms of action of short-chain fatty acids on adipose tissue cells, primarily on the processes of regulation of adipogenesis and lipid metabolism, can contribute to more successful prevention and treatment of obesity through changes in the composition of intestinal microbiota and its products that can be controlled by different dietary regimes.",
publisher = "Niš : Educo Events",
journal = "Zbornik radova: 1. Srpski kongres dečje endokrinologije "Čigota 2018" sa međunarodnim učešćem; 2018 Apr 26-28; Zlatibor, Serbia",
title = "The role of gut microbiota in the development of obesity: molecular mechanisms and therapeutic implications, Uloga crevne mikrobiote u razvoju gojaznosti: molekularni mehanizmi i terapijske implikacije",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5649"
}

Ignjatović, Đ.,& Đorđević, A.. (2018). The role of gut microbiota in the development of obesity: molecular mechanisms and therapeutic implications. in Zbornik radova: 1. Srpski kongres dečje endokrinologije "Čigota 2018" sa međunarodnim učešćem; 2018 Apr 26-28; Zlatibor, Serbia
Niš : Educo Events., 20.
https://hdl.handle.net/21.15107/rcub_ibiss_5649

Ignjatović Đ, Đorđević A. The role of gut microbiota in the development of obesity: molecular mechanisms and therapeutic implications. in Zbornik radova: 1. Srpski kongres dečje endokrinologije "Čigota 2018" sa međunarodnim učešćem; 2018 Apr 26-28; Zlatibor, Serbia. 2018;:20.
https://hdl.handle.net/21.15107/rcub_ibiss_5649 .

Ignjatović, Đurđica, Đorđević, Ana, "The role of gut microbiota in the development of obesity: molecular mechanisms and therapeutic implications" in Zbornik radova: 1. Srpski kongres dečje endokrinologije "Čigota 2018" sa međunarodnim učešćem; 2018 Apr 26-28; Zlatibor, Serbia (2018):20,
https://hdl.handle.net/21.15107/rcub_ibiss_5649 .

TY  - CONF
AU  - Gligorovska, Ljupka
AU  - Đorđević, Ana
AU  - Bursać, Biljana
AU  - Veličković, Nataša
AU  - Ignjatović, Đurđica
AU  - Tomić, Mirko
AU  - Matić, Gordana
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5443
AB  - Introduction: The macrophage migration inhibitory factor (MIF) is involved in the regulation of glucose metabolism in the hippocampus, while fructose overconsumption has been associated with changes in hippocampal insulin sensitivity and synaptic plasticity, resulting in disturbances in learning and memory. Therefore, we hypothesized that MIF defficiency in combination with fructose diet (FD) may affect insulin sensitivity and hippocampal synaptic plasticity, thus leading to behavioral changes.
Methods: The effects of 9-week 20% FD on energy intake and insulin sensitivity in wild type (WT) and MIF deficient (MIF−/−) C57Bl/6J male mice were analyzed. Inhibitory Ser307 phosphorylation of insulin receptor substrate 1 (IRS-1) was used as hallmark of hippocampal insulin resistance. Novel object recognition test (NOR) was used to assess exploratory behavior. Synaptic plasticity was estimated by polysialylated-neural cell adhesion molecule (PSA-NCAM) protein level.
Results: WT and MIF−/− mice on fructose diet had increased energy intake, while systemic insulin sensitivity was disturbed in all MIF−/− mice. Hippocampal pIRS-1 Ser307 was elevated in all animals compared to WT on standard diet (SD). MIF−/− animals on SD showed impaired recognition memory in NOR. Finally, although PSA-NCAM was decreased in WT animals on FD and MIF−/− on SD, its level in fructose-fed MIF−/− mice was the same as in the WT on SD.
Conclusion: These preliminary results show that both MIF defficiency and fructose caloric overload are implicated in impairment of systemic and hippocampal insulin sensitivity. However, behavioral changes observed in MIF−/− mice were normalized after fructose feeding, which coincided with the changes in synaptosomal PSA-NCAM protein level.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia
T1  - Effects of macrophage migration inhibitory factor deficiency and fructose-enriched diet on systemic and hippocampal insulin sesitivity, behavior and hippocampal plasticity in male mice
SP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5443
ER  - 

@conference{
author = "Gligorovska, Ljupka and Đorđević, Ana and Bursać, Biljana and Veličković, Nataša and Ignjatović, Đurđica and Tomić, Mirko and Matić, Gordana",
year = "2017",
abstract = "Introduction: The macrophage migration inhibitory factor (MIF) is involved in the regulation of glucose metabolism in the hippocampus, while fructose overconsumption has been associated with changes in hippocampal insulin sensitivity and synaptic plasticity, resulting in disturbances in learning and memory. Therefore, we hypothesized that MIF defficiency in combination with fructose diet (FD) may affect insulin sensitivity and hippocampal synaptic plasticity, thus leading to behavioral changes.
Methods: The effects of 9-week 20% FD on energy intake and insulin sensitivity in wild type (WT) and MIF deficient (MIF−/−) C57Bl/6J male mice were analyzed. Inhibitory Ser307 phosphorylation of insulin receptor substrate 1 (IRS-1) was used as hallmark of hippocampal insulin resistance. Novel object recognition test (NOR) was used to assess exploratory behavior. Synaptic plasticity was estimated by polysialylated-neural cell adhesion molecule (PSA-NCAM) protein level.
Results: WT and MIF−/− mice on fructose diet had increased energy intake, while systemic insulin sensitivity was disturbed in all MIF−/− mice. Hippocampal pIRS-1 Ser307 was elevated in all animals compared to WT on standard diet (SD). MIF−/− animals on SD showed impaired recognition memory in NOR. Finally, although PSA-NCAM was decreased in WT animals on FD and MIF−/− on SD, its level in fructose-fed MIF−/− mice was the same as in the WT on SD.
Conclusion: These preliminary results show that both MIF defficiency and fructose caloric overload are implicated in impairment of systemic and hippocampal insulin sensitivity. However, behavioral changes observed in MIF−/− mice were normalized after fructose feeding, which coincided with the changes in synaptosomal PSA-NCAM protein level.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia",
title = "Effects of macrophage migration inhibitory factor deficiency and fructose-enriched diet on systemic and hippocampal insulin sesitivity, behavior and hippocampal plasticity in male mice",
pages = "97",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5443"
}

Gligorovska, L., Đorđević, A., Bursać, B., Veličković, N., Ignjatović, Đ., Tomić, M.,& Matić, G.. (2017). Effects of macrophage migration inhibitory factor deficiency and fructose-enriched diet on systemic and hippocampal insulin sesitivity, behavior and hippocampal plasticity in male mice. in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 97.
https://hdl.handle.net/21.15107/rcub_ibiss_5443

Gligorovska L, Đorđević A, Bursać B, Veličković N, Ignjatović Đ, Tomić M, Matić G. Effects of macrophage migration inhibitory factor deficiency and fructose-enriched diet on systemic and hippocampal insulin sesitivity, behavior and hippocampal plasticity in male mice. in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia. 2017;:97.
https://hdl.handle.net/21.15107/rcub_ibiss_5443 .

Gligorovska, Ljupka, Đorđević, Ana, Bursać, Biljana, Veličković, Nataša, Ignjatović, Đurđica, Tomić, Mirko, Matić, Gordana, "Effects of macrophage migration inhibitory factor deficiency and fructose-enriched diet on systemic and hippocampal insulin sesitivity, behavior and hippocampal plasticity in male mice" in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia (2017):97,
https://hdl.handle.net/21.15107/rcub_ibiss_5443 .

TY  - JOUR
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović, Snježana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Maria
PY  - 2017
UR  - http://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0483-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2768
AB  - Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.
T2  - Lipids in Health and Disease
T1  - Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes
IS  - 1
VL  - 16
DO  - 10.1186/s12944-017-0483-9
SP  - 94
EP  - 94
ER  - 

@article{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović, Snježana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Maria",
year = "2017",
abstract = "Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.",
journal = "Lipids in Health and Disease",
title = "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes",
number = "1",
volume = "16",
doi = "10.1186/s12944-017-0483-9",
pages = "94-94"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović, S., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2017). Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease, 16(1), 94-94.
https://doi.org/10.1186/s12944-017-0483-9

Ranković S, Popović T, Debeljak Martačić J, Petrović S, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease. 2017;16(1):94-94.
doi:10.1186/s12944-017-0483-9 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović, Snježana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Maria, "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes" in Lipids in Health and Disease, 16, no. 1 (2017):94-94,
https://doi.org/10.1186/s12944-017-0483-9 . .

TY  - JOUR
AU  - Đorđević, Ana
AU  - Bursać, Biljana
AU  - Veličković, Nataša
AU  - Gligorovska, Ljupka
AU  - Ignjatović, Đurđica
AU  - Tomić, Mirko
AU  - Matić, Gordana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0018506X17301277
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2855
AB  - Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine well known for its role in inflammation enhancement. However, a growing body of evidence is emerging on its role in energy metabolism in insulin sensitive tissues such as hippocampus, a brain region implicated in cognition, learning and memory. We hypothesized that genetic deletion of MIF may result in the specific behavioral changes, which may be linked tо impairments in brain or systemic insulin sensitivity by possible changes of the hippocampal synaptic plasticity. To assess memory, exploratory behavior and anxiety, three behavioral tests were applied on Mif gene-deficient (MIF −/− ) and “wild type” C57BL/6J mice (WT). The parameters of systemic and hippocampal insulin sensitivity were also determined. The impact of MIF deficiency on hippocampal plasticity was evaluated by analyzing the level of synaptosomal polysialylated-neural cell adhesion molecule (PSA-NCAM) plasticity marker and mRNA levels of different neurotrophic factors. The results showed that MIF −/− mice exhibit emphasized anxiety-like behaviors, as well as impaired recognition memory, which may be hippocampus-dependent. This behavioral phenotype was associated with impaired systemic insulin sensitivity and attenuated hippocampal insulin sensitivity, characterized by increased inhibitory Ser 307 phosphorylation of insulin receptor substrate 1 (IRS1). Finally, MIF −/− mice displayed a decreased hippocampal PSA-NCAM level and unchanged Bdnf, NT-3, NT-4 and Igf-1 mRNA levels. The results suggest that the lack of MIF leads to disturbances of systemic and hippocampal insulin sensitivity, which are possibly responsible for memory deficits and anxiety, most likely through decreased PSA-NCAM-mediated neuroplasticity rather than through neurotrophic factors.
T2  - Hormones and Behavior
T1  - Disturbances of systemic and hippocampal insulin sensitivity in macrophage migration inhibitory factor (MIF) knockout male mice lead to behavioral changes associated with decreased PSA-NCAM levels
VL  - 96
DO  - 10.1016/j.yhbeh.2017.09.008
SP  - 95
EP  - 103
ER  - 

@article{
author = "Đorđević, Ana and Bursać, Biljana and Veličković, Nataša and Gligorovska, Ljupka and Ignjatović, Đurđica and Tomić, Mirko and Matić, Gordana",
year = "2017",
abstract = "Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine well known for its role in inflammation enhancement. However, a growing body of evidence is emerging on its role in energy metabolism in insulin sensitive tissues such as hippocampus, a brain region implicated in cognition, learning and memory. We hypothesized that genetic deletion of MIF may result in the specific behavioral changes, which may be linked tо impairments in brain or systemic insulin sensitivity by possible changes of the hippocampal synaptic plasticity. To assess memory, exploratory behavior and anxiety, three behavioral tests were applied on Mif gene-deficient (MIF −/− ) and “wild type” C57BL/6J mice (WT). The parameters of systemic and hippocampal insulin sensitivity were also determined. The impact of MIF deficiency on hippocampal plasticity was evaluated by analyzing the level of synaptosomal polysialylated-neural cell adhesion molecule (PSA-NCAM) plasticity marker and mRNA levels of different neurotrophic factors. The results showed that MIF −/− mice exhibit emphasized anxiety-like behaviors, as well as impaired recognition memory, which may be hippocampus-dependent. This behavioral phenotype was associated with impaired systemic insulin sensitivity and attenuated hippocampal insulin sensitivity, characterized by increased inhibitory Ser 307 phosphorylation of insulin receptor substrate 1 (IRS1). Finally, MIF −/− mice displayed a decreased hippocampal PSA-NCAM level and unchanged Bdnf, NT-3, NT-4 and Igf-1 mRNA levels. The results suggest that the lack of MIF leads to disturbances of systemic and hippocampal insulin sensitivity, which are possibly responsible for memory deficits and anxiety, most likely through decreased PSA-NCAM-mediated neuroplasticity rather than through neurotrophic factors.",
journal = "Hormones and Behavior",
title = "Disturbances of systemic and hippocampal insulin sensitivity in macrophage migration inhibitory factor (MIF) knockout male mice lead to behavioral changes associated with decreased PSA-NCAM levels",
volume = "96",
doi = "10.1016/j.yhbeh.2017.09.008",
pages = "95-103"
}

Đorđević, A., Bursać, B., Veličković, N., Gligorovska, L., Ignjatović, Đ., Tomić, M.,& Matić, G.. (2017). Disturbances of systemic and hippocampal insulin sensitivity in macrophage migration inhibitory factor (MIF) knockout male mice lead to behavioral changes associated with decreased PSA-NCAM levels. in Hormones and Behavior, 96, 95-103.
https://doi.org/10.1016/j.yhbeh.2017.09.008

Đorđević A, Bursać B, Veličković N, Gligorovska L, Ignjatović Đ, Tomić M, Matić G. Disturbances of systemic and hippocampal insulin sensitivity in macrophage migration inhibitory factor (MIF) knockout male mice lead to behavioral changes associated with decreased PSA-NCAM levels. in Hormones and Behavior. 2017;96:95-103.
doi:10.1016/j.yhbeh.2017.09.008 .

Đorđević, Ana, Bursać, Biljana, Veličković, Nataša, Gligorovska, Ljupka, Ignjatović, Đurđica, Tomić, Mirko, Matić, Gordana, "Disturbances of systemic and hippocampal insulin sensitivity in macrophage migration inhibitory factor (MIF) knockout male mice lead to behavioral changes associated with decreased PSA-NCAM levels" in Hormones and Behavior, 96 (2017):95-103,
https://doi.org/10.1016/j.yhbeh.2017.09.008 . .

TY  - JOUR
AU  - Zec, Manja
AU  - Debeljak Martačić, Jasmina
AU  - Ranković, Slavica
AU  - Pokimica, Biljana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Glibetić, Maria
AU  - Popović, Tamara
PY  - 2017
UR  - http://www.degruyter.com/view/j/acve.2017.67.issue-1/acve-2017-0010/acve-2017-0010.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2731
AB  - A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.
T2  - Acta Veterinaria
T1  - Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats
IS  - 1
VL  - 67
DO  - 10.1515/acve-2017-0010
SP  - 107
EP  - 120
ER  - 

@article{
author = "Zec, Manja and Debeljak Martačić, Jasmina and Ranković, Slavica and Pokimica, Biljana and Tomić, Mirko and Ignjatović, Đurđica and Glibetić, Maria and Popović, Tamara",
year = "2017",
abstract = "A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.",
journal = "Acta Veterinaria",
title = "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats",
number = "1",
volume = "67",
doi = "10.1515/acve-2017-0010",
pages = "107-120"
}

Zec, M., Debeljak Martačić, J., Ranković, S., Pokimica, B., Tomić, M., Ignjatović, Đ., Glibetić, M.,& Popović, T.. (2017). Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria, 67(1), 107-120.
https://doi.org/10.1515/acve-2017-0010

Zec M, Debeljak Martačić J, Ranković S, Pokimica B, Tomić M, Ignjatović Đ, Glibetić M, Popović T. Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria. 2017;67(1):107-120.
doi:10.1515/acve-2017-0010 .

Zec, Manja, Debeljak Martačić, Jasmina, Ranković, Slavica, Pokimica, Biljana, Tomić, Mirko, Ignjatović, Đurđica, Glibetić, Maria, Popović, Tamara, "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats" in Acta Veterinaria, 67, no. 1 (2017):107-120,
https://doi.org/10.1515/acve-2017-0010 . .

TY  - JOUR
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Krstić Milošević, Dijana
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Glibetić, Marija
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/123456789/3894
AB  - The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of
Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic
compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable
health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate
the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice
reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic
compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three
various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 +
20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution
was significantly elevated from the second or third week onward, which was most expressed in the ARO
group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field
tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also
expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate
the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml
per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The
in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the
possible in vivo mechanisms for such behavioral actions.
PB  - Royal Society of Chemistry
T2  - Food & Function
T1  - Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice
VL  - 7
DO  - 10.1039/c6fo00321d
SP  - 3111
EP  - 3120
ER  - 

@article{
author = "Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Krstić Milošević, Dijana and Ranković, Slavica and Popović, Tamara and Glibetić, Marija",
year = "2016",
abstract = "The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of
Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic
compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable
health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate
the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice
reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic
compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three
various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 +
20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution
was significantly elevated from the second or third week onward, which was most expressed in the ARO
group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field
tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also
expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate
the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml
per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The
in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the
possible in vivo mechanisms for such behavioral actions.",
publisher = "Royal Society of Chemistry",
journal = "Food & Function",
title = "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice",
volume = "7",
doi = "10.1039/c6fo00321d",
pages = "3111-3120"
}

Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Krstić Milošević, D., Ranković, S., Popović, T.,& Glibetić, M.. (2016). Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function
Royal Society of Chemistry., 7, 3111-3120.
https://doi.org/10.1039/c6fo00321d

Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Krstić Milošević D, Ranković S, Popović T, Glibetić M. Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function. 2016;7:3111-3120.
doi:10.1039/c6fo00321d .

Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Krstić Milošević, Dijana, Ranković, Slavica, Popović, Tamara, Glibetić, Marija, "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice" in Food & Function, 7 (2016):3111-3120,
https://doi.org/10.1039/c6fo00321d . .

TY  - JOUR
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Krstić Milošević, Dijana
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Glibetić, Marija
PY  - 2016
UR  - https://pubs.rsc.org/en/content/articlelanding/2016/FO/C6FO00321D#!divAbstract
UR  - https://radar.ibiss.bg.ac.rs/123456789/3893
AB  - The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 + 20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution was significantly elevated from the second or third week onward, which was most expressed in the ARO group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the possible in vivo mechanisms for such behavioral actions.
PB  - Royal Society of Chemistry
T2  - Food & Function
T1  - Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice
VL  - 7
DO  - 10.1039/c6fo00321d
SP  - 3111
EP  - 3120
ER  - 

@article{
author = "Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Krstić Milošević, Dijana and Ranković, Slavica and Popović, Tamara and Glibetić, Marija",
year = "2016",
abstract = "The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 + 20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution was significantly elevated from the second or third week onward, which was most expressed in the ARO group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the possible in vivo mechanisms for such behavioral actions.",
publisher = "Royal Society of Chemistry",
journal = "Food & Function",
title = "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice",
volume = "7",
doi = "10.1039/c6fo00321d",
pages = "3111-3120"
}

Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Krstić Milošević, D., Ranković, S., Popović, T.,& Glibetić, M.. (2016). Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function
Royal Society of Chemistry., 7, 3111-3120.
https://doi.org/10.1039/c6fo00321d

Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Krstić Milošević D, Ranković S, Popović T, Glibetić M. Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function. 2016;7:3111-3120.
doi:10.1039/c6fo00321d .

Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Krstić Milošević, Dijana, Ranković, Slavica, Popović, Tamara, Glibetić, Marija, "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice" in Food & Function, 7 (2016):3111-3120,
https://doi.org/10.1039/c6fo00321d . .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović Oggiano, Gordana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Miler, Marko
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6347
AB  - Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
T1  - Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6347
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović Oggiano, Gordana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Miler, Marko and Glibetić, Marija",
year = "2015",
abstract = "Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia",
title = "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6347"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović Oggiano, G., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Miler, M.,& Glibetić, M.. (2015). Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347

Ranković S, Popović T, Debeljak Martačić J, Petrović Oggiano G, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Miler M, Glibetić M. Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia. 2015;:139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović Oggiano, Gordana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Miler, Marko, Glibetić, Marija, "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats" in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia (2015):139-139,
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Slavica
AU  - Glibetić, Marija
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6349
AB  - Aronia melanocarpa fruits are among the richest plant sources of phenolic substances, mainly anthocyanins. Aronia fruit juice and derived flavonoids were evaluated in a number of studies for high antioxidative activity, whose sequels were many beneficial physiological and metabolic effects, like hepato-protective, anti-inflammatory and antitumor. This study was designed to explore the effects of one month intensive consumption of aronia juice on phospholipid compositions in rat liver. Three experimental groups of young male rats were being supplied for 34 days with different drinking solutions ad libitum: 1. (ARO group) 20% (m/m) master aronia juice in tap water; 2. (MIX) 5% master aronia juice + 15% juice reconstruct (without flavonoids); 3. (PLC) 20% juice reconstruct. The animals were sacrificed on day 35 and their livers were weighed and used for analysis. The average mass of liver in ARO group was somewhat higher (13-14%) than in other two groups. In comparison with PLC, both experimental groups exhibited significantly lower proportions of stearic acid (18:0) and arachidonic acid (20:4), whilst the level of MUFA was increased only in MIX group on account of vaccenic acid elevation (18:1(7)). Also, there was a certain increase of omega-3 PUFA with a decline of omega 6/omega-3 PUFA ratio in both groups that consumed aronia juice. All these changes in the liver phospholipid compositions, which were induced by everyday drinking of flavonoid-rich aronia juice, appeared to be favorable regarding the healthy ratios of the liver fatty acid contents.
PB  - elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver
SP  - 86
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6349
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Slavica and Glibetić, Marija and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Tomić, Mirko",
year = "2015",
abstract = "Aronia melanocarpa fruits are among the richest plant sources of phenolic substances, mainly anthocyanins. Aronia fruit juice and derived flavonoids were evaluated in a number of studies for high antioxidative activity, whose sequels were many beneficial physiological and metabolic effects, like hepato-protective, anti-inflammatory and antitumor. This study was designed to explore the effects of one month intensive consumption of aronia juice on phospholipid compositions in rat liver. Three experimental groups of young male rats were being supplied for 34 days with different drinking solutions ad libitum: 1. (ARO group) 20% (m/m) master aronia juice in tap water; 2. (MIX) 5% master aronia juice + 15% juice reconstruct (without flavonoids); 3. (PLC) 20% juice reconstruct. The animals were sacrificed on day 35 and their livers were weighed and used for analysis. The average mass of liver in ARO group was somewhat higher (13-14%) than in other two groups. In comparison with PLC, both experimental groups exhibited significantly lower proportions of stearic acid (18:0) and arachidonic acid (20:4), whilst the level of MUFA was increased only in MIX group on account of vaccenic acid elevation (18:1(7)). Also, there was a certain increase of omega-3 PUFA with a decline of omega 6/omega-3 PUFA ratio in both groups that consumed aronia juice. All these changes in the liver phospholipid compositions, which were induced by everyday drinking of flavonoid-rich aronia juice, appeared to be favorable regarding the healthy ratios of the liver fatty acid contents.",
publisher = "elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver",
pages = "86-87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6349"
}

Ranković, S., Popović, T., Debeljak Martačić, S., Glibetić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Tomić, M.. (2015). Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 86-87.
https://hdl.handle.net/21.15107/rcub_ibiss_6349

Ranković S, Popović T, Debeljak Martačić S, Glibetić M, Ignjatović Đ, Tovilović-Kovačević G, Tomić M. Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:86-87.
https://hdl.handle.net/21.15107/rcub_ibiss_6349 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Slavica, Glibetić, Marija, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Tomić, Mirko, "Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):86-87,
https://hdl.handle.net/21.15107/rcub_ibiss_6349 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Slavica
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6344
AB  - The fruits of Aronia melanocarpa are rich in anthocyanins, plant pigments with a number of registered beneficial effects on human health (e.g. antioxidative, anti-inflammatory, immunomodulatory). Some limited studies have also shown their effects on behavior and cognitive functions in experimental animals. This study was designed to explore the effects of unlimited consumption of diluted aronia juice on rat behavior. Young male Wistar rats were divided into control group (CTL; N=12) with tap water provided ad libitum, and 3 experimental groups (N=8) supplied with drinking solutions of 20% mixtures (m/m) in tap water: (ARO) 100% master aronia juice; (MIX) 25% master aronia juice + 75% placebo solution (juice reconstruct without flavonoids); (PLC) 100% placebo. Rats were being allowed to drink these solutions without limits for 34 days, where on days 31-34 three behavioral tests were performed. These tests revealed that only ARO group exhibited significant behavioral changes: augmented locomotion in an open field test, anxiolytic-like action in elevated plus maze, as well as an increase in active swimming in forced swimming test (which is relevant to antidepressant-like action). These results suggest that everyday drinking of diluted aronia juice (between 6 and 12 g of master aronia juice daily per rat) with high flavonoid content (about 0.12%) for about a month, may induce several stimulant behavioral actions in experimental animals.
PB  - Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6344
ER  - 

@conference{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Tomić, Mirko and Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Slavica and Glibetić, Marija",
year = "2015",
abstract = "The fruits of Aronia melanocarpa are rich in anthocyanins, plant pigments with a number of registered beneficial effects on human health (e.g. antioxidative, anti-inflammatory, immunomodulatory). Some limited studies have also shown their effects on behavior and cognitive functions in experimental animals. This study was designed to explore the effects of unlimited consumption of diluted aronia juice on rat behavior. Young male Wistar rats were divided into control group (CTL; N=12) with tap water provided ad libitum, and 3 experimental groups (N=8) supplied with drinking solutions of 20% mixtures (m/m) in tap water: (ARO) 100% master aronia juice; (MIX) 25% master aronia juice + 75% placebo solution (juice reconstruct without flavonoids); (PLC) 100% placebo. Rats were being allowed to drink these solutions without limits for 34 days, where on days 31-34 three behavioral tests were performed. These tests revealed that only ARO group exhibited significant behavioral changes: augmented locomotion in an open field test, anxiolytic-like action in elevated plus maze, as well as an increase in active swimming in forced swimming test (which is relevant to antidepressant-like action). These results suggest that everyday drinking of diluted aronia juice (between 6 and 12 g of master aronia juice daily per rat) with high flavonoid content (about 0.12%) for about a month, may induce several stimulant behavioral actions in experimental animals.",
publisher = "Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6344"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Tomić, M., Ranković, S., Popović, T., Debeljak Martačić, S.,& Glibetić, M.. (2015). The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"., 78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6344

Ignjatović Đ, Tovilović-Kovačević G, Tomić M, Ranković S, Popović T, Debeljak Martačić S, Glibetić M. The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6344 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Tomić, Mirko, Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Slavica, Glibetić, Marija, "The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):78-79,
https://hdl.handle.net/21.15107/rcub_ibiss_6344 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Slavica
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6340
AB  - The aim of this study was to assess how the unlimited drinking of Aronia melanocarpa fruit juice, rich in phenolic substances (1.177 g L-¹ gallic acid equivalents), may affect the levels of several biochemical markers in the rat plasma: glucose (Glu), total cholesterol (ChT) and its fractions (HDL and LDL), triglycerides (TG) and transaminases (ALT and AST). Young male rats were being supplied with 3 combinations of juice solutions in drinking water for 34 days, and their blood samples were collected for analysis after animal sacrifices on day 35. The experimental groups contained 20% juice solutions in tap water of: (ARO) 100% master aronia juice, (MIX) 25% master aronia juice + 75% juice reconstruct (without flavonoids), and (PLC) 100% juice reconstruct, and they were compared with a (CTL) control group on pure tap water. Biochemical analyses of plasma on Cobas c-111 analyzer showed a significant 20% decrease of Glu in ARO group in comparison to CTL. ChT was significantly higher in MIX and PLC in relation to both CTL and ARO groups, which arose mainly from LDL elevations. Also, there was a certain (30%), but insignificant increase of TG in MIX and PLC vs. CTL. The effects of aronia on transaminases were registered only for ALT, whose level in ARO was about 3-fold of that in CTL group. In conclusion, everyday free drinking of flavonoid-rich aronia juice solutions appeared to have global hypoglycemic effect in normal rats and, also, certain hypolipidemic effects, when hyperlipidemia was induced probably with higher sugar consumption.
PB  - Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6340
ER  - 

@conference{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Tomić, Mirko and Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Slavica and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to assess how the unlimited drinking of Aronia melanocarpa fruit juice, rich in phenolic substances (1.177 g L-¹ gallic acid equivalents), may affect the levels of several biochemical markers in the rat plasma: glucose (Glu), total cholesterol (ChT) and its fractions (HDL and LDL), triglycerides (TG) and transaminases (ALT and AST). Young male rats were being supplied with 3 combinations of juice solutions in drinking water for 34 days, and their blood samples were collected for analysis after animal sacrifices on day 35. The experimental groups contained 20% juice solutions in tap water of: (ARO) 100% master aronia juice, (MIX) 25% master aronia juice + 75% juice reconstruct (without flavonoids), and (PLC) 100% juice reconstruct, and they were compared with a (CTL) control group on pure tap water. Biochemical analyses of plasma on Cobas c-111 analyzer showed a significant 20% decrease of Glu in ARO group in comparison to CTL. ChT was significantly higher in MIX and PLC in relation to both CTL and ARO groups, which arose mainly from LDL elevations. Also, there was a certain (30%), but insignificant increase of TG in MIX and PLC vs. CTL. The effects of aronia on transaminases were registered only for ALT, whose level in ARO was about 3-fold of that in CTL group. In conclusion, everyday free drinking of flavonoid-rich aronia juice solutions appeared to have global hypoglycemic effect in normal rats and, also, certain hypolipidemic effects, when hyperlipidemia was induced probably with higher sugar consumption.",
publisher = "Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6340"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Tomić, M., Ranković, S., Popović, T., Debeljak Martačić, S.,& Glibetić, M.. (2015). Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”., 78-78.
https://hdl.handle.net/21.15107/rcub_ibiss_6340

Ignjatović Đ, Tovilović-Kovačević G, Tomić M, Ranković S, Popović T, Debeljak Martačić S, Glibetić M. Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:78-78.
https://hdl.handle.net/21.15107/rcub_ibiss_6340 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Tomić, Mirko, Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Slavica, Glibetić, Marija, "Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):78-78,
https://hdl.handle.net/21.15107/rcub_ibiss_6340 .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6348
AB  - Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.
PB  - Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology
C3  - Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
T1  - Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6348
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Marija",
year = "2015",
abstract = "Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.",
publisher = "Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology",
journal = "Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia",
title = "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6348"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2015). Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology., 85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348

Ranković S, Popović T, Debeljak Martačić J, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia. 2015;:85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Marija, "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats" in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia (2015):85-85,
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .