TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5364
AB  - This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5364
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Václav and Raspor, Martin and Sajid, Sumbal and Savić, Jelena and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5364"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Savić, J., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364

Ćosić T, Motyka V, Raspor M, Sajid S, Savić J, Devrnja N, Dobrev PI, Ninković S. Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

Ćosić, Tatjana, Motyka, Václav, Raspor, Martin, Sajid, Sumbal, Savić, Jelena, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .