TY  - JOUR
AU  - Nešović, Marija
AU  - Divac Rankov, Aleksandra
AU  - Podolski-Renić, Ana
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Mancini, Arianna
AU  - Schenone, Silvia
AU  - Pešić, Milica
AU  - Dinić, Jelena
PY  - 2020
UR  - https://www.mdpi.com/2072-6694/12/6/1570
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3818
AB  - Glioblastoma (GBM), as the most aggressive brain tumor, displays a high expression of Src tyrosine kinase, which is involved in the survival, migration, and invasiveness of tumor cells. Thus, Src emerged as a potential target for GBM therapy. The effects of Src inhibitors pyrazolo[3,4-d]pyrimidines, Si306 and its prodrug pro-Si306 were investigated in human GBM cell lines (U87 and U87-TxR) and three primary GBM cell cultures. Primary GBM cells were more resistant to Si306 and pro-Si306 according to the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. However, the ability of all GBM cells to degrade the extracellular matrix was considerably compromised after Si306 and pro-Si306 applications. Besides reducing the phosphorylation of Src and its downstream signaling pathway components, both compounds decreased the phosphorylated form of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) expression, showing the potential to suppress the aggressiveness of GBM. In vivo, Si306 and pro-Si306 displayed an anti-invasive effect against U87 xenografts in the zebrafish embryo model. Considering that Si306 and pro-Si306 are able to cross the blood-brain barrier and suppress the spread of GBM cells, we anticipate their clinical testing in the near future. Moreover, the prodrug showed similar efficacy to the drug, implying the rationality of its use in clinical settings.
PB  - Basel : MDPI
T2  - Cancers (Basel)
T1  - Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo
IS  - 6
VL  - 12
DO  - 10.3390/cancers12061570
SP  - 1570
ER  - 

@article{
author = "Nešović, Marija and Divac Rankov, Aleksandra and Podolski-Renić, Ana and Nikolić, Igor and Tasić, Goran and Mancini, Arianna and Schenone, Silvia and Pešić, Milica and Dinić, Jelena",
year = "2020",
abstract = "Glioblastoma (GBM), as the most aggressive brain tumor, displays a high expression of Src tyrosine kinase, which is involved in the survival, migration, and invasiveness of tumor cells. Thus, Src emerged as a potential target for GBM therapy. The effects of Src inhibitors pyrazolo[3,4-d]pyrimidines, Si306 and its prodrug pro-Si306 were investigated in human GBM cell lines (U87 and U87-TxR) and three primary GBM cell cultures. Primary GBM cells were more resistant to Si306 and pro-Si306 according to the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. However, the ability of all GBM cells to degrade the extracellular matrix was considerably compromised after Si306 and pro-Si306 applications. Besides reducing the phosphorylation of Src and its downstream signaling pathway components, both compounds decreased the phosphorylated form of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) expression, showing the potential to suppress the aggressiveness of GBM. In vivo, Si306 and pro-Si306 displayed an anti-invasive effect against U87 xenografts in the zebrafish embryo model. Considering that Si306 and pro-Si306 are able to cross the blood-brain barrier and suppress the spread of GBM cells, we anticipate their clinical testing in the near future. Moreover, the prodrug showed similar efficacy to the drug, implying the rationality of its use in clinical settings.",
publisher = "Basel : MDPI",
journal = "Cancers (Basel)",
title = "Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo",
number = "6",
volume = "12",
doi = "10.3390/cancers12061570",
pages = "1570"
}

Nešović, M., Divac Rankov, A., Podolski-Renić, A., Nikolić, I., Tasić, G., Mancini, A., Schenone, S., Pešić, M.,& Dinić, J.. (2020). Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo. in Cancers (Basel)
Basel : MDPI., 12(6), 1570.
https://doi.org/10.3390/cancers12061570

Nešović M, Divac Rankov A, Podolski-Renić A, Nikolić I, Tasić G, Mancini A, Schenone S, Pešić M, Dinić J. Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo. in Cancers (Basel). 2020;12(6):1570.
doi:10.3390/cancers12061570 .

Nešović, Marija, Divac Rankov, Aleksandra, Podolski-Renić, Ana, Nikolić, Igor, Tasić, Goran, Mancini, Arianna, Schenone, Silvia, Pešić, Milica, Dinić, Jelena, "Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo" in Cancers (Basel), 12, no. 6 (2020):1570,
https://doi.org/10.3390/cancers12061570 . .

TY  - JOUR
AU  - Nestorović Živković, Jasmina
AU  - Živković, Suzana
AU  - Šiler, Branislav
AU  - Aničić, Neda
AU  - Dmitrović, Slavica
AU  - Divac-Rankov, Aleksandra
AU  - Giba, Zlatko
AU  - Mišić, Danijela
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700026N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3017
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1881
AB  - Methanol extracts of three endemic Nepeta species were analyzed for their main secondary metabolites, terpenes and phenolics, and further investigated for antioxidant capacity and embryonic toxicity in zebrafish. UHPLC/DAD/(±) HESI-MS/MS analysis showed that the dominant compound in N. rtanjensis was trans,cis-nepetalactone, the cis,trans isomer of this monoterpene lactone was dominant in N. sibirica, while nepetalactone was detected only in traces in N. nervosa. In all investigated species, rosmarinic acid was the dominant phenolic compound, while other identified phenolic acids (chlorogenic, neochlorogenic and caffeic) were present in considerably lower amounts. ABTS and DPPH assays showed that the methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa possessed strong antioxidant activities, with the FRAP assay revealing high ferric-reducing abilities for all three tested species. Such a strong antioxidant potential, especially as manifested in the DPPH and FRAP assays, can be attributed to phenolic acids, and in the first place to rosmarinic acid. Increased lethality of zebrafish embryos in any of the treatments was not observed, but several toxic effects on embryonic development were recorded, such as pericardial and yolk sac edema. As in other Nepeta species, the three studied endemic species possessed a great potential for food conservation or as medicinal supplements if applied in optimized concentrations; however, alternative sources of plant material (e.g. field cultivation) should be established bearing in mind their vulnerability in nature.
T2  - Archives of Biological Sciences
T2  - Archives of Biological Sciences
T1  - Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents
IS  - 1
VL  - 70
DO  - 10.2298/ABS170616026N
SP  - 63
EP  - 76
ER  - 

@article{
author = "Nestorović Živković, Jasmina and Živković, Suzana and Šiler, Branislav and Aničić, Neda and Dmitrović, Slavica and Divac-Rankov, Aleksandra and Giba, Zlatko and Mišić, Danijela",
year = "2018",
abstract = "Methanol extracts of three endemic Nepeta species were analyzed for their main secondary metabolites, terpenes and phenolics, and further investigated for antioxidant capacity and embryonic toxicity in zebrafish. UHPLC/DAD/(±) HESI-MS/MS analysis showed that the dominant compound in N. rtanjensis was trans,cis-nepetalactone, the cis,trans isomer of this monoterpene lactone was dominant in N. sibirica, while nepetalactone was detected only in traces in N. nervosa. In all investigated species, rosmarinic acid was the dominant phenolic compound, while other identified phenolic acids (chlorogenic, neochlorogenic and caffeic) were present in considerably lower amounts. ABTS and DPPH assays showed that the methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa possessed strong antioxidant activities, with the FRAP assay revealing high ferric-reducing abilities for all three tested species. Such a strong antioxidant potential, especially as manifested in the DPPH and FRAP assays, can be attributed to phenolic acids, and in the first place to rosmarinic acid. Increased lethality of zebrafish embryos in any of the treatments was not observed, but several toxic effects on embryonic development were recorded, such as pericardial and yolk sac edema. As in other Nepeta species, the three studied endemic species possessed a great potential for food conservation or as medicinal supplements if applied in optimized concentrations; however, alternative sources of plant material (e.g. field cultivation) should be established bearing in mind their vulnerability in nature.",
journal = "Archives of Biological Sciences, Archives of Biological Sciences",
title = "Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents",
number = "1",
volume = "70",
doi = "10.2298/ABS170616026N",
pages = "63-76"
}

Nestorović Živković, J., Živković, S., Šiler, B., Aničić, N., Dmitrović, S., Divac-Rankov, A., Giba, Z.,& Mišić, D.. (2018). Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents. in Archives of Biological Sciences, 70(1), 63-76.
https://doi.org/10.2298/ABS170616026N

Nestorović Živković J, Živković S, Šiler B, Aničić N, Dmitrović S, Divac-Rankov A, Giba Z, Mišić D. Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents. in Archives of Biological Sciences. 2018;70(1):63-76.
doi:10.2298/ABS170616026N .

Nestorović Živković, Jasmina, Živković, Suzana, Šiler, Branislav, Aničić, Neda, Dmitrović, Slavica, Divac-Rankov, Aleksandra, Giba, Zlatko, Mišić, Danijela, "Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents" in Archives of Biological Sciences, 70, no. 1 (2018):63-76,
https://doi.org/10.2298/ABS170616026N . .

TY  - JOUR
AU  - Babić, Tamara
AU  - Dinić, Jelena
AU  - Stojković Burić, Sonja
AU  - Hadzic, Stefan
AU  - Pešić, Milica
AU  - Radojković, Dragica
AU  - Divac Rankov, Aleksandra
PY  - 2018
UR  - https://akjournals.com/view/journals/018/69/4/article-p395.xml
UR  - https://radar.ibiss.bg.ac.rs/123456789/3881
AB  - Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.
PB  - Budapest : Akadémiai Kiadó
T2  - Acta Biologica Hungarica
T1  - Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models
IS  - 4
VL  - 69
DO  - 10.1556/018.69.2018.4.3
SP  - 395
EP  - 410
ER  - 

@article{
author = "Babić, Tamara and Dinić, Jelena and Stojković Burić, Sonja and Hadzic, Stefan and Pešić, Milica and Radojković, Dragica and Divac Rankov, Aleksandra",
year = "2018",
abstract = "Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.",
publisher = "Budapest : Akadémiai Kiadó",
journal = "Acta Biologica Hungarica",
title = "Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models",
number = "4",
volume = "69",
doi = "10.1556/018.69.2018.4.3",
pages = "395-410"
}

Babić, T., Dinić, J., Stojković Burić, S., Hadzic, S., Pešić, M., Radojković, D.,& Divac Rankov, A.. (2018). Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models. in Acta Biologica Hungarica
Budapest : Akadémiai Kiadó., 69(4), 395-410.
https://doi.org/10.1556/018.69.2018.4.3

Babić T, Dinić J, Stojković Burić S, Hadzic S, Pešić M, Radojković D, Divac Rankov A. Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models. in Acta Biologica Hungarica. 2018;69(4):395-410.
doi:10.1556/018.69.2018.4.3 .

Babić, Tamara, Dinić, Jelena, Stojković Burić, Sonja, Hadzic, Stefan, Pešić, Milica, Radojković, Dragica, Divac Rankov, Aleksandra, "Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models" in Acta Biologica Hungarica, 69, no. 4 (2018):395-410,
https://doi.org/10.1556/018.69.2018.4.3 . .

TY  - JOUR
AU  - Babić, Tamara
AU  - Dinić, Jelena
AU  - Stojković Burić, Sonja
AU  - Hadžić, Stefan
AU  - Pešić, Milica
AU  - Radojković, Dragica
AU  - Divac Rankov, Aleksandra
PY  - 2018
UR  - https://www.akademiai.com/doi/10.1556/018.69.2018.4.3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3231
AB  - Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.
T2  - Acta Biologica Hungarica
T1  - Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models.
IS  - 4
VL  - 69
DO  - 10.1556/018.69.2018.4.3
SP  - 395
EP  - 410
ER  - 

@article{
author = "Babić, Tamara and Dinić, Jelena and Stojković Burić, Sonja and Hadžić, Stefan and Pešić, Milica and Radojković, Dragica and Divac Rankov, Aleksandra",
year = "2018",
abstract = "Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.",
journal = "Acta Biologica Hungarica",
title = "Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models.",
number = "4",
volume = "69",
doi = "10.1556/018.69.2018.4.3",
pages = "395-410"
}

Babić, T., Dinić, J., Stojković Burić, S., Hadžić, S., Pešić, M., Radojković, D.,& Divac Rankov, A.. (2018). Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models.. in Acta Biologica Hungarica, 69(4), 395-410.
https://doi.org/10.1556/018.69.2018.4.3

Babić T, Dinić J, Stojković Burić S, Hadžić S, Pešić M, Radojković D, Divac Rankov A. Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models.. in Acta Biologica Hungarica. 2018;69(4):395-410.
doi:10.1556/018.69.2018.4.3 .

Babić, Tamara, Dinić, Jelena, Stojković Burić, Sonja, Hadžić, Stefan, Pešić, Milica, Radojković, Dragica, Divac Rankov, Aleksandra, "Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models." in Acta Biologica Hungarica, 69, no. 4 (2018):395-410,
https://doi.org/10.1556/018.69.2018.4.3 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617304486
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2832
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
DO  - 10.1016/j.jff.2017.07.060
SP  - 249
EP  - 262
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Gašić, Uroš and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
doi = "10.1016/j.jff.2017.07.060",
pages = "249-262"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Gašić, U., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods, 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtić B, Nikolovski N, Stanisavljević S, Gašić U, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Gašić, Uroš, Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - GEN
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
AU  - Petrić, Marija
AU  - Radojković, Dragica
AU  - Pešić, Milica
AU  - Dinić, Jelena
PY  - 2017
UR  - http://link.springer.com/10.1007/s00418-017-1590-4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2796
AB  - Autophagy is linked to multiple cancer-related signaling pathways, and represents a defense mechanism for cancer cells under therapeutic stress. The crosstalk between apoptosis and autophagy is essential for both tumorigenesis and embryonic development. We studied the influence of autophagy on cell survival in pro-apoptotic conditions induced by anticancer drugs in three model systems: human cancer cells (NCI-H460, COR-L23 and U87), human normal cells (HaCaT and MRC-5) and zebrafish embryos (Danio rerio). Autophagy induction with AZD2014 and tamoxifen antagonized the pro-apoptotic effect of chemotherapeutics doxorubicin and cisplatin in cell lines, while autophagy inhibition by wortmannin and chloroquine synergized the action of both anticancer agents. This effect was further verified by assessing cleaved caspase-3 and PARP-1 levels. Autophagy inhibitors significantly increased both apoptotic markers when applied in combination with doxorubicin while autophagy inducers had the opposite effect. In a similar manner, autophagy induction in zebrafish embryos prevented cisplatin-induced apoptosis in the tail region while autophagy inhibition increased cell death in the tail and retina of cisplatin-treated animals. Autophagy modulation with direct inhibitors of the PI3kinase/Akt/mTOR pathway (AZD2014 and wortmannin) triggered the cellular response to anticancer drugs more effectively in NCI-H460 and zebrafish embryonic models compared to HaCaT suggesting that these modulators are selective towards rapidly proliferating cells. Therefore, evaluating the autophagic properties of chemotherapeutics could help determine more accurately the fate of different cell types under treatment. Our study underlines the importance of testing autophagic activity of potential anticancer agents in a comparative approach to develop more rational anticancer therapeutic strategies.
T2  - Histochemistry and Cell Biology
T1  - Targeting autophagy to modulate cell survival: a comparative analysis in cancer, normal and embryonic cells
DO  - 10.1007/s00418-017-1590-4
ER  - 

@misc{
author = "Divac Rankov, Aleksandra and Ljujić, Mila and Petrić, Marija and Radojković, Dragica and Pešić, Milica and Dinić, Jelena",
year = "2017",
abstract = "Autophagy is linked to multiple cancer-related signaling pathways, and represents a defense mechanism for cancer cells under therapeutic stress. The crosstalk between apoptosis and autophagy is essential for both tumorigenesis and embryonic development. We studied the influence of autophagy on cell survival in pro-apoptotic conditions induced by anticancer drugs in three model systems: human cancer cells (NCI-H460, COR-L23 and U87), human normal cells (HaCaT and MRC-5) and zebrafish embryos (Danio rerio). Autophagy induction with AZD2014 and tamoxifen antagonized the pro-apoptotic effect of chemotherapeutics doxorubicin and cisplatin in cell lines, while autophagy inhibition by wortmannin and chloroquine synergized the action of both anticancer agents. This effect was further verified by assessing cleaved caspase-3 and PARP-1 levels. Autophagy inhibitors significantly increased both apoptotic markers when applied in combination with doxorubicin while autophagy inducers had the opposite effect. In a similar manner, autophagy induction in zebrafish embryos prevented cisplatin-induced apoptosis in the tail region while autophagy inhibition increased cell death in the tail and retina of cisplatin-treated animals. Autophagy modulation with direct inhibitors of the PI3kinase/Akt/mTOR pathway (AZD2014 and wortmannin) triggered the cellular response to anticancer drugs more effectively in NCI-H460 and zebrafish embryonic models compared to HaCaT suggesting that these modulators are selective towards rapidly proliferating cells. Therefore, evaluating the autophagic properties of chemotherapeutics could help determine more accurately the fate of different cell types under treatment. Our study underlines the importance of testing autophagic activity of potential anticancer agents in a comparative approach to develop more rational anticancer therapeutic strategies.",
journal = "Histochemistry and Cell Biology",
title = "Targeting autophagy to modulate cell survival: a comparative analysis in cancer, normal and embryonic cells",
doi = "10.1007/s00418-017-1590-4"
}

Divac Rankov, A., Ljujić, M., Petrić, M., Radojković, D., Pešić, M.,& Dinić, J.. (2017). Targeting autophagy to modulate cell survival: a comparative analysis in cancer, normal and embryonic cells. in Histochemistry and Cell Biology.
https://doi.org/10.1007/s00418-017-1590-4

Divac Rankov A, Ljujić M, Petrić M, Radojković D, Pešić M, Dinić J. Targeting autophagy to modulate cell survival: a comparative analysis in cancer, normal and embryonic cells. in Histochemistry and Cell Biology. 2017;.
doi:10.1007/s00418-017-1590-4 .

Divac Rankov, Aleksandra, Ljujić, Mila, Petrić, Marija, Radojković, Dragica, Pešić, Milica, Dinić, Jelena, "Targeting autophagy to modulate cell survival: a comparative analysis in cancer, normal and embryonic cells" in Histochemistry and Cell Biology (2017),
https://doi.org/10.1007/s00418-017-1590-4 . .

TY  - JOUR
AU  - Ljujić, Mila
AU  - Mijatović, Sanja
AU  - Bulatović, Mirna
AU  - Mojić, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Radojković, Dragica
AU  - Topić, Aleksandra
PY  - 2017
UR  - http://link.springer.com/10.1007/s12253-016-0104-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2729
AB  - Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.
T2  - Pathology & Oncology Research
T1  - Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines
IS  - 2
VL  - 23
DO  - 10.1007/s12253-016-0104-3
SP  - 335
EP  - 343
ER  - 

@article{
author = "Ljujić, Mila and Mijatović, Sanja and Bulatović, Mirna and Mojić, Marija and Maksimović-Ivanić, Danijela and Radojković, Dragica and Topić, Aleksandra",
year = "2017",
abstract = "Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.",
journal = "Pathology & Oncology Research",
title = "Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines",
number = "2",
volume = "23",
doi = "10.1007/s12253-016-0104-3",
pages = "335-343"
}

Ljujić, M., Mijatović, S., Bulatović, M., Mojić, M., Maksimović-Ivanić, D., Radojković, D.,& Topić, A.. (2017). Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines. in Pathology & Oncology Research, 23(2), 335-343.
https://doi.org/10.1007/s12253-016-0104-3

Ljujić M, Mijatović S, Bulatović M, Mojić M, Maksimović-Ivanić D, Radojković D, Topić A. Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines. in Pathology & Oncology Research. 2017;23(2):335-343.
doi:10.1007/s12253-016-0104-3 .

Ljujić, Mila, Mijatović, Sanja, Bulatović, Mirna, Mojić, Marija, Maksimović-Ivanić, Danijela, Radojković, Dragica, Topić, Aleksandra, "Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines" in Pathology & Oncology Research, 23, no. 2 (2017):335-343,
https://doi.org/10.1007/s12253-016-0104-3 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Despotović, Jovana
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6050
AB  - Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4(+) T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, NFκB, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.
PB  - American Chemical Society
T2  - Journal of Agricultural and Food Chemistry
T1  - Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells
IS  - 24
VL  - 64
DO  - 10.1021/acs.jafc.6b00951
SP  - 4900
EP  - 4907
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Despotović, Jovana and Miljković, Đorđe and Timotijević, Gordana",
year = "2016",
abstract = "Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4(+) T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, NFκB, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.",
publisher = "American Chemical Society",
journal = "Journal of Agricultural and Food Chemistry",
title = "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells",
number = "24",
volume = "64",
doi = "10.1021/acs.jafc.6b00951",
pages = "4900-4907"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Despotović, J., Miljković, Đ.,& Timotijević, G.. (2016). Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry
American Chemical Society., 64(24), 4900-4907.
https://doi.org/10.1021/acs.jafc.6b00951

Jevtić B, Nikolovski N, Stanisavljević S, Despotović J, Miljković Đ, Timotijević G. Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry. 2016;64(24):4900-4907.
doi:10.1021/acs.jafc.6b00951 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Despotović, Jovana, Miljković, Đorđe, Timotijević, Gordana, "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells" in Journal of Agricultural and Food Chemistry, 64, no. 24 (2016):4900-4907,
https://doi.org/10.1021/acs.jafc.6b00951 . .

TY  - JOUR
AU  - Ljujic, Mila
AU  - Mijatović, Sanja
AU  - Bulatović, Mirna
AU  - Mojić, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Radojkovic, Dragica
AU  - Topic, Aleksandra
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4166
AB  - Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-κB. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.
PB  - Moskva: Izdatelstvo Nauka
T2  - Molekuliarnaia biologiia
T1  - Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)
IS  - 1
VL  - 50
DO  - 10.7868/S0026898416010122
SP  - 174
EP  - 178
ER  - 

@article{
author = "Ljujic, Mila and Mijatović, Sanja and Bulatović, Mirna and Mojić, Marija and Maksimović-Ivanić, Danijela and Radojkovic, Dragica and Topic, Aleksandra",
year = "2016",
abstract = "Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-κB. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.",
publisher = "Moskva: Izdatelstvo Nauka",
journal = "Molekuliarnaia biologiia",
title = "Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)",
number = "1",
volume = "50",
doi = "10.7868/S0026898416010122",
pages = "174-178"
}

Ljujic, M., Mijatović, S., Bulatović, M., Mojić, M., Maksimović-Ivanić, D., Radojkovic, D.,& Topic, A.. (2016). Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molekuliarnaia biologiia
Moskva: Izdatelstvo Nauka., 50(1), 174-178.
https://doi.org/10.7868/S0026898416010122

Ljujic M, Mijatović S, Bulatović M, Mojić M, Maksimović-Ivanić D, Radojkovic D, Topic A. Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molekuliarnaia biologiia. 2016;50(1):174-178.
doi:10.7868/S0026898416010122 .

Ljujic, Mila, Mijatović, Sanja, Bulatović, Mirna, Mojić, Marija, Maksimović-Ivanić, Danijela, Radojkovic, Dragica, Topic, Aleksandra, "Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)" in Molekuliarnaia biologiia, 50, no. 1 (2016):174-178,
https://doi.org/10.7868/S0026898416010122 . .

TY  - JOUR
AU  - Đorđević, Valentina
AU  - Timotijević, Gordana
AU  - Pruner, Iva
AU  - Radojković, Dragica
AU  - Milovanović, Boško
AU  - Miljković, Đorđe
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/503
AB  - Background: The mutant CCR5Δ32 allele confers resistance to HIV infection. Several hypotheses regarding its origin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was introduced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was determined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 distribution within the continent. Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild­type CCR5 sequence and a product of 231 bp was obtained from the truncated CCR5Δ32 sequence. Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homozygous and 8.52% are heterozygous for CCR5Δ32. Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, considering ethnically related populations.
AB  - Uvod: Nosioci alela CCR5Δ32 su relativno rezistentni na infekciju HIV-om. Postoji nekoliko hipoteza o poreklu i održanju ovog alela u ljudskoj populaciji. Pretpostavlja se da je mutacija Δ32 nastala u populaciji severne ili istočne Evrope i da se potom proširila ka jugu. Iako je učestalost CCR5Δ32 određena u mnogim evropskim populacijama, dodatni podaci su neophodni za formiranje sveobuhvatne slike o distribuciji CCR5Δ32 u Evropi. Zbog toga smo u našoj studiji odredili učestalost CCR5Δ32 u srpskoj populaciji, za koju do ovog rada nisu postojali takvi podaci. Metode: DNK je izolovana iz periferne krvi 352 osobe. U reakciji lančanog umnožavanja korišćeni su prajmeri specifični za gen CCR5. Dobijen je proizvod od 263 bp na osnovu matrice 'wild type', sekvence CCR5 gena, a proizvod od 231 bp na osnovu okrnjene sekvence gena CCR5 (CCR5Δ32). Ukupna učestalost alela CCR5Δ32 u srpskoj populaciji iznosi 4,55%. Rezultati: Od ukupnog broja analiziranih osoba, identifikovano je 8,52% heterozigotnih i 0,57% homozigotnih nosilaca za ovaj alel. Zaključak: Utvrđena učestalost alela CCR5Δ32 u srpskoj populaciji je neočekivano niska, u poređenju sa učestalošću u ostalim slovenskim populacijama.
T2  - Journal of Medical Biochemistry
T1  - Učestalost alela CCR5Δ32 u srpskoj populaciji
T1  - The frequency of allele CCR5Δ32 in a Serbian population
IS  - 4
VL  - 32
DO  - 10.2478/jomb-2013-0030
SP  - 368
EP  - 374
ER  - 

@article{
author = "Đorđević, Valentina and Timotijević, Gordana and Pruner, Iva and Radojković, Dragica and Milovanović, Boško and Miljković, Đorđe",
year = "2013, 2013",
abstract = "Background: The mutant CCR5Δ32 allele confers resistance to HIV infection. Several hypotheses regarding its origin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was introduced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was determined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 distribution within the continent. Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild­type CCR5 sequence and a product of 231 bp was obtained from the truncated CCR5Δ32 sequence. Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homozygous and 8.52% are heterozygous for CCR5Δ32. Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, considering ethnically related populations., Uvod: Nosioci alela CCR5Δ32 su relativno rezistentni na infekciju HIV-om. Postoji nekoliko hipoteza o poreklu i održanju ovog alela u ljudskoj populaciji. Pretpostavlja se da je mutacija Δ32 nastala u populaciji severne ili istočne Evrope i da se potom proširila ka jugu. Iako je učestalost CCR5Δ32 određena u mnogim evropskim populacijama, dodatni podaci su neophodni za formiranje sveobuhvatne slike o distribuciji CCR5Δ32 u Evropi. Zbog toga smo u našoj studiji odredili učestalost CCR5Δ32 u srpskoj populaciji, za koju do ovog rada nisu postojali takvi podaci. Metode: DNK je izolovana iz periferne krvi 352 osobe. U reakciji lančanog umnožavanja korišćeni su prajmeri specifični za gen CCR5. Dobijen je proizvod od 263 bp na osnovu matrice 'wild type', sekvence CCR5 gena, a proizvod od 231 bp na osnovu okrnjene sekvence gena CCR5 (CCR5Δ32). Ukupna učestalost alela CCR5Δ32 u srpskoj populaciji iznosi 4,55%. Rezultati: Od ukupnog broja analiziranih osoba, identifikovano je 8,52% heterozigotnih i 0,57% homozigotnih nosilaca za ovaj alel. Zaključak: Utvrđena učestalost alela CCR5Δ32 u srpskoj populaciji je neočekivano niska, u poređenju sa učestalošću u ostalim slovenskim populacijama.",
journal = "Journal of Medical Biochemistry",
title = "Učestalost alela CCR5Δ32 u srpskoj populaciji, The frequency of allele CCR5Δ32 in a Serbian population",
number = "4",
volume = "32",
doi = "10.2478/jomb-2013-0030",
pages = "368-374"
}

Đorđević, V., Timotijević, G., Pruner, I., Radojković, D., Milovanović, B.,& Miljković, Đ.. (2013). Učestalost alela CCR5Δ32 u srpskoj populaciji. in Journal of Medical Biochemistry, 32(4), 368-374.
https://doi.org/10.2478/jomb-2013-0030

Đorđević V, Timotijević G, Pruner I, Radojković D, Milovanović B, Miljković Đ. Učestalost alela CCR5Δ32 u srpskoj populaciji. in Journal of Medical Biochemistry. 2013;32(4):368-374.
doi:10.2478/jomb-2013-0030 .

Đorđević, Valentina, Timotijević, Gordana, Pruner, Iva, Radojković, Dragica, Milovanović, Boško, Miljković, Đorđe, "Učestalost alela CCR5Δ32 u srpskoj populaciji" in Journal of Medical Biochemistry, 32, no. 4 (2013):368-374,
https://doi.org/10.2478/jomb-2013-0030 . .