TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Vojnović-Milutinović, Danijela
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Renko, Kostja
AU  - Wirth, Eva Katrin
AU  - Janković, Snežana
AU  - Kӧhrle, Josef
AU  - Milošević, Verica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S053155651630585X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2722
AB  - Soy-food and its isoflavones, genistein (G) and daidzein (D), were reported to exert mild cholesterol-lowering effect, but the underlying mechanism is still unclear. In this research, first we studied age-related alterations in hepatic cholesterol metabolism of acyclic middle-aged (MA) female rats. Then we tested if purified isoflavones may prevent or reverse these changes, and whether putative changes in hepatic thyroid hormone availability may be associated with this effect. Serum and hepatic total cholesterol (TChol), bile acid and cholesterol precursors, as well as serum TSH and T4 concentrations, hepatic deiodinase (Dio) 1 enzyme activity and MCT8 protein expression were determined by comparing data obtained for MA with young adult (YA) intact (IC) females. Effects of subcutaneously administered G or D (35 mg/kg) to MA rats were evaluated versus vehicle-treated MA females. MA IC females were characterized by: higher (p < 0.05) serum TChol, lower (p < 0.05) hepatic TChol and its biosynthetic precursors, lower (p < 0.05) hepatic 7α-hydroxycholesterol but elevated (p < 0.05) 27- and 24-hydroxycholesterol in comparison to YA IC. Both isoflavone treatments decreased (p < 0.05) hepatic 27-hydroxycholesterol, G being more effective than D, without affecting any other parameter of Chol metabolism. Only G elevated hepatic Dio1 activity (p < 0.05). In conclusion, age-related hypercholesteremia was associated with lower hepatic Chol synthesis and shift from main neutral (lower 7α-hydroxycholesterol) to alternative acidic pathway (higher 27-hydroxycholesterol) of Chol degradation to bile acid. Both isoflavones lowered hepatic 27-hydroxycholesterol, which may be considered beneficial. Only G treatment increased hepatic Dio1 activity, thus indicating local increase in thyroid hormones, obviously insufficient to induce prominent cholesterol-lowering effect.
T2  - Experimental Gerontology
T1  - Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats
VL  - 92
DO  - 10.1016/j.exger.2017.03.016
SP  - 74
EP  - 81
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Jarić, Ivana and Miler, Marko and Vojnović-Milutinović, Danijela and Filipović, Branko and Ajdžanović, Vladimir and Renko, Kostja and Wirth, Eva Katrin and Janković, Snežana and Kӧhrle, Josef and Milošević, Verica",
year = "2017",
abstract = "Soy-food and its isoflavones, genistein (G) and daidzein (D), were reported to exert mild cholesterol-lowering effect, but the underlying mechanism is still unclear. In this research, first we studied age-related alterations in hepatic cholesterol metabolism of acyclic middle-aged (MA) female rats. Then we tested if purified isoflavones may prevent or reverse these changes, and whether putative changes in hepatic thyroid hormone availability may be associated with this effect. Serum and hepatic total cholesterol (TChol), bile acid and cholesterol precursors, as well as serum TSH and T4 concentrations, hepatic deiodinase (Dio) 1 enzyme activity and MCT8 protein expression were determined by comparing data obtained for MA with young adult (YA) intact (IC) females. Effects of subcutaneously administered G or D (35 mg/kg) to MA rats were evaluated versus vehicle-treated MA females. MA IC females were characterized by: higher (p < 0.05) serum TChol, lower (p < 0.05) hepatic TChol and its biosynthetic precursors, lower (p < 0.05) hepatic 7α-hydroxycholesterol but elevated (p < 0.05) 27- and 24-hydroxycholesterol in comparison to YA IC. Both isoflavone treatments decreased (p < 0.05) hepatic 27-hydroxycholesterol, G being more effective than D, without affecting any other parameter of Chol metabolism. Only G elevated hepatic Dio1 activity (p < 0.05). In conclusion, age-related hypercholesteremia was associated with lower hepatic Chol synthesis and shift from main neutral (lower 7α-hydroxycholesterol) to alternative acidic pathway (higher 27-hydroxycholesterol) of Chol degradation to bile acid. Both isoflavones lowered hepatic 27-hydroxycholesterol, which may be considered beneficial. Only G treatment increased hepatic Dio1 activity, thus indicating local increase in thyroid hormones, obviously insufficient to induce prominent cholesterol-lowering effect.",
journal = "Experimental Gerontology",
title = "Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats",
volume = "92",
doi = "10.1016/j.exger.2017.03.016",
pages = "74-81"
}

Šošić-Jurjević, B., Lütjohann, D., Jarić, I., Miler, M., Vojnović-Milutinović, D., Filipović, B., Ajdžanović, V., Renko, K., Wirth, E. K., Janković, S., Kӧhrle, J.,& Milošević, V.. (2017). Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats. in Experimental Gerontology, 92, 74-81.
https://doi.org/10.1016/j.exger.2017.03.016

Šošić-Jurjević B, Lütjohann D, Jarić I, Miler M, Vojnović-Milutinović D, Filipović B, Ajdžanović V, Renko K, Wirth EK, Janković S, Kӧhrle J, Milošević V. Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats. in Experimental Gerontology. 2017;92:74-81.
doi:10.1016/j.exger.2017.03.016 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Jarić, Ivana, Miler, Marko, Vojnović-Milutinović, Danijela, Filipović, Branko, Ajdžanović, Vladimir, Renko, Kostja, Wirth, Eva Katrin, Janković, Snežana, Kӧhrle, Josef, Milošević, Verica, "Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats" in Experimental Gerontology, 92 (2017):74-81,
https://doi.org/10.1016/j.exger.2017.03.016 . .