TY  - CONF
AU  - Pajić, Tanja
AU  - Stevanović, Katarina
AU  - Todorović, Nataša
AU  - Krmpot, Aleksandar
AU  - Rabasović, Mihailo
AU  - Lazović, Vladimir
AU  - Pantelić, Dejan
AU  - Jelenković, Branislav
AU  - Živić, Miroslav
PY  - 2018
UR  - https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwj1h4zoze6BAxWUtqQKHec6BAsQFnoECBsQAQ&url=https%3A%2F%2Fskbs.sk%2Fwp-content%2Fuploads%2F2018%2F05%2Frbc_2018.pdf&usg=AOvVaw3s3FIWs-7Q4jE-gsL6G_U6&opi=89978449
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6292
AB  - Application of patch-clamp method for investigation of membrane ion channels of filamentous
fungi is nontrivial task due to presence of chitinous cell wall. Complete removal of the
wall patch is needed to make the membrane accessible to glass pipette. We use the model
filamentous fungus organism, Phycomyces blakesleeanus which is undertaken to the cell
surgery procedure by means of tightly focused femtosecond laser beam. The hyphae were
grown on glass coverslips coated with collagen plasmolysed and imaged by homemade nonlinear
laser scanning microscope by detecting two photon excitation fluorescence signal.
Although intrinsic autofluorescence of chitin enables imaging of the cell wall the hyphae
were stained by Calcofluor White dye prior to the imaging in order to improve signal to
noise ratio. Ti:Sa laser, used for both imaging and surgery, was operating at 730nm, with
76MHz repetition rate and 160fs pulse duration. Carl Zeiss, EC Plan-NEOFLUAR, 401.3
oil immersion objective was used for tight focusing of the laser beam and for the collection
of the fluorescence signal. A visible interference filter (415nm - 685nm) was placed in front
of detector in order to remove scattered laser light. The successful cutting of cell wall could
be achieved within the range of laser intensities and cutting speeds (dwell times). The
hyphae were kept in azide throughout the experiment in order to block the regeneration
of the cell wall. After the surgery, hyphae were slowly deplasmolysed to induce exit of a
portion of the protoplast through the laser made incision in the cell wall.
PB  - Ljubljana: Slovenian Biophysical Society
C3  - Book of abstracts: 8th Regional Biophysics Conference: RBC2018; 2018 May 16-20; Zreče, Slovenia
T1  - Successful Ti: Sapphire laser cell surgery of Phycomyces blakesleeanus cell wall
SP  - PS-43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6292
ER  - 

@conference{
author = "Pajić, Tanja and Stevanović, Katarina and Todorović, Nataša and Krmpot, Aleksandar and Rabasović, Mihailo and Lazović, Vladimir and Pantelić, Dejan and Jelenković, Branislav and Živić, Miroslav",
year = "2018",
abstract = "Application of patch-clamp method for investigation of membrane ion channels of filamentous
fungi is nontrivial task due to presence of chitinous cell wall. Complete removal of the
wall patch is needed to make the membrane accessible to glass pipette. We use the model
filamentous fungus organism, Phycomyces blakesleeanus which is undertaken to the cell
surgery procedure by means of tightly focused femtosecond laser beam. The hyphae were
grown on glass coverslips coated with collagen plasmolysed and imaged by homemade nonlinear
laser scanning microscope by detecting two photon excitation fluorescence signal.
Although intrinsic autofluorescence of chitin enables imaging of the cell wall the hyphae
were stained by Calcofluor White dye prior to the imaging in order to improve signal to
noise ratio. Ti:Sa laser, used for both imaging and surgery, was operating at 730nm, with
76MHz repetition rate and 160fs pulse duration. Carl Zeiss, EC Plan-NEOFLUAR, 401.3
oil immersion objective was used for tight focusing of the laser beam and for the collection
of the fluorescence signal. A visible interference filter (415nm - 685nm) was placed in front
of detector in order to remove scattered laser light. The successful cutting of cell wall could
be achieved within the range of laser intensities and cutting speeds (dwell times). The
hyphae were kept in azide throughout the experiment in order to block the regeneration
of the cell wall. After the surgery, hyphae were slowly deplasmolysed to induce exit of a
portion of the protoplast through the laser made incision in the cell wall.",
publisher = "Ljubljana: Slovenian Biophysical Society",
journal = "Book of abstracts: 8th Regional Biophysics Conference: RBC2018; 2018 May 16-20; Zreče, Slovenia",
title = "Successful Ti: Sapphire laser cell surgery of Phycomyces blakesleeanus cell wall",
pages = "PS-43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6292"
}

Pajić, T., Stevanović, K., Todorović, N., Krmpot, A., Rabasović, M., Lazović, V., Pantelić, D., Jelenković, B.,& Živić, M.. (2018). Successful Ti: Sapphire laser cell surgery of Phycomyces blakesleeanus cell wall. in Book of abstracts: 8th Regional Biophysics Conference: RBC2018; 2018 May 16-20; Zreče, Slovenia
Ljubljana: Slovenian Biophysical Society., PS-43.
https://hdl.handle.net/21.15107/rcub_ibiss_6292

Pajić T, Stevanović K, Todorović N, Krmpot A, Rabasović M, Lazović V, Pantelić D, Jelenković B, Živić M. Successful Ti: Sapphire laser cell surgery of Phycomyces blakesleeanus cell wall. in Book of abstracts: 8th Regional Biophysics Conference: RBC2018; 2018 May 16-20; Zreče, Slovenia. 2018;:PS-43.
https://hdl.handle.net/21.15107/rcub_ibiss_6292 .

Pajić, Tanja, Stevanović, Katarina, Todorović, Nataša, Krmpot, Aleksandar, Rabasović, Mihailo, Lazović, Vladimir, Pantelić, Dejan, Jelenković, Branislav, Živić, Miroslav, "Successful Ti: Sapphire laser cell surgery of Phycomyces blakesleeanus cell wall" in Book of abstracts: 8th Regional Biophysics Conference: RBC2018; 2018 May 16-20; Zreče, Slovenia (2018):PS-43,
https://hdl.handle.net/21.15107/rcub_ibiss_6292 .

TY  - JOUR
AU  - Pavlović, Danica
AU  - Vasiljević, Darko
AU  - Salatić, Branislav
AU  - Lazović, Vladimir
AU  - Dikić, Goran
AU  - Tomić, Ljubiša
AU  - Ćurčić, Srećko
AU  - Milovanović, Petar
AU  - Todorović, Dajana
AU  - Pantelić, Dejan V.
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0306456518301402?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3124
AB  - The insect cuticle serves a multitude of purposes, including: mechanical and thermal protection, water-repelling, acoustic signal absorption and coloration. The influence of cuticular structures on infrared radiation exchange and thermal balance is still largely unexplored. Here we report on the micro- and nanostructured setae covering the elytra of the longicorn beetle Rosalia alpina (Linnaeus, 1758) (Coleoptera: Cerambycidae) that help the insect to survive in hot, summer environments. In the visible part of the spectrum, scale-like setae, covering the black patches of the elytra, efficiently absorb light due to the radiation trap effect. In the infrared part of the spectrum, setae of the whole elytra significantly contribute to the radiative heat exchange. From the biological point of view, insect elytra facilitate camouflage, enable rapid heating to the optimum body temperature and prevent overheating by emitting excess thermal energy.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae)
VL  - 76
DO  - 10.1016/J.JTHERBIO.2018.07.014
SP  - 126
EP  - 138
ER  - 

@article{
author = "Pavlović, Danica and Vasiljević, Darko and Salatić, Branislav and Lazović, Vladimir and Dikić, Goran and Tomić, Ljubiša and Ćurčić, Srećko and Milovanović, Petar and Todorović, Dajana and Pantelić, Dejan V.",
year = "2018",
abstract = "The insect cuticle serves a multitude of purposes, including: mechanical and thermal protection, water-repelling, acoustic signal absorption and coloration. The influence of cuticular structures on infrared radiation exchange and thermal balance is still largely unexplored. Here we report on the micro- and nanostructured setae covering the elytra of the longicorn beetle Rosalia alpina (Linnaeus, 1758) (Coleoptera: Cerambycidae) that help the insect to survive in hot, summer environments. In the visible part of the spectrum, scale-like setae, covering the black patches of the elytra, efficiently absorb light due to the radiation trap effect. In the infrared part of the spectrum, setae of the whole elytra significantly contribute to the radiative heat exchange. From the biological point of view, insect elytra facilitate camouflage, enable rapid heating to the optimum body temperature and prevent overheating by emitting excess thermal energy.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae)",
volume = "76",
doi = "10.1016/J.JTHERBIO.2018.07.014",
pages = "126-138"
}

Pavlović, D., Vasiljević, D., Salatić, B., Lazović, V., Dikić, G., Tomić, L., Ćurčić, S., Milovanović, P., Todorović, D.,& Pantelić, D. V.. (2018). Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae). in Journal of Thermal Biology
Elsevier Ltd.., 76, 126-138.
https://doi.org/10.1016/J.JTHERBIO.2018.07.014

Pavlović D, Vasiljević D, Salatić B, Lazović V, Dikić G, Tomić L, Ćurčić S, Milovanović P, Todorović D, Pantelić DV. Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae). in Journal of Thermal Biology. 2018;76:126-138.
doi:10.1016/J.JTHERBIO.2018.07.014 .

Pavlović, Danica, Vasiljević, Darko, Salatić, Branislav, Lazović, Vladimir, Dikić, Goran, Tomić, Ljubiša, Ćurčić, Srećko, Milovanović, Petar, Todorović, Dajana, Pantelić, Dejan V., "Photonic structures improve radiative heat exchange of Rosalia alpina (Coleoptera: Cerambycidae)" in Journal of Thermal Biology, 76 (2018):126-138,
https://doi.org/10.1016/J.JTHERBIO.2018.07.014 . .

TY  - JOUR
AU  - Pavlović, Danica
AU  - Petković, Branka
AU  - Ćurčić, Srećko
AU  - Todorović, Dajana
AU  - Vesović, Nikola
AU  - Pantelić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2016
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/eea.12470
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3779
AB  - The aim of this study was to investigate the effects of a static magnetic field on six behavioral parameters (travel distance, average speed while in motion, travel distance of the head, body rotations, time in movement, and immobility time) of the ground beetle Laemostenus (Pristonychus) punctatus (Dejean) (Coleoptera: Carabidae). Adults of this troglophilic and guanophilic beetle were randomly divided into two groups, the first exposed to a static magnetic field of 110 mT for 5 h, and the second a control group. Beetle behavior after these 5 h was monitored in an open-field test for 12 min and analyzed using ANY-maze software. Exposure to a static magnetic field of 110 mT increased motor activity (travel distance and average speed while in motion) in the first 4 min. After that there were no significant differences. We conclude that the applied static magnetic field affects motor activity of adult specimens of L. (P.) punctatus, and we discuss the mechanism, possibly through acting on the control centers responsible for orientation and movement.
PB  - Blackwell Publishing Ltd.
T2  - Entomologia Experimentalis et Applicata
T1  - Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT
IS  - 2
VL  - 160
DO  - 10.1111/eea.12470
SP  - 188
EP  - 194
ER  - 

@article{
author = "Pavlović, Danica and Petković, Branka and Ćurčić, Srećko and Todorović, Dajana and Vesović, Nikola and Pantelić, Dejan and Perić Mataruga, Vesna",
year = "2016",
abstract = "The aim of this study was to investigate the effects of a static magnetic field on six behavioral parameters (travel distance, average speed while in motion, travel distance of the head, body rotations, time in movement, and immobility time) of the ground beetle Laemostenus (Pristonychus) punctatus (Dejean) (Coleoptera: Carabidae). Adults of this troglophilic and guanophilic beetle were randomly divided into two groups, the first exposed to a static magnetic field of 110 mT for 5 h, and the second a control group. Beetle behavior after these 5 h was monitored in an open-field test for 12 min and analyzed using ANY-maze software. Exposure to a static magnetic field of 110 mT increased motor activity (travel distance and average speed while in motion) in the first 4 min. After that there were no significant differences. We conclude that the applied static magnetic field affects motor activity of adult specimens of L. (P.) punctatus, and we discuss the mechanism, possibly through acting on the control centers responsible for orientation and movement.",
publisher = "Blackwell Publishing Ltd.",
journal = "Entomologia Experimentalis et Applicata",
title = "Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT",
number = "2",
volume = "160",
doi = "10.1111/eea.12470",
pages = "188-194"
}

Pavlović, D., Petković, B., Ćurčić, S., Todorović, D., Vesović, N., Pantelić, D.,& Perić Mataruga, V.. (2016). Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT. in Entomologia Experimentalis et Applicata
Blackwell Publishing Ltd.., 160(2), 188-194.
https://doi.org/10.1111/eea.12470

Pavlović D, Petković B, Ćurčić S, Todorović D, Vesović N, Pantelić D, Perić Mataruga V. Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT. in Entomologia Experimentalis et Applicata. 2016;160(2):188-194.
doi:10.1111/eea.12470 .

Pavlović, Danica, Petković, Branka, Ćurčić, Srećko, Todorović, Dajana, Vesović, Nikola, Pantelić, Dejan, Perić Mataruga, Vesna, "Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT" in Entomologia Experimentalis et Applicata, 160, no. 2 (2016):188-194,
https://doi.org/10.1111/eea.12470 . .