TY  - JOUR
AU  - Zogović, Nevena
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Harhaji-Trajković, Ljubica
AU  - Miljković, Đorđe
AU  - Trajković, Vladimir
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6013
AB  - The role of iron in 6-hydroxydopamine (6-OHDA) toxicity towards astrocytes was investigated in vitro using rat primary astrocytes, rat astrocytoma cell line C6, and human astrocytoma cell line U251. The assessment of mitochondrial respiration or lactate dehydrogenase release has shown a dose-dependent decrease in the viability of astrocytes treated with 6-OHDA, which coincided with DNA fragmentation and the changes in cellular morphology. This was a consequence of the oxidative stress mediated by 6-OHDA autoxidation products hydrogen peroxide, superoxide anion, and hydroxyl radical. Both FeSO(4) and FeCl(3) markedly alleviated detrimental effects of 6-OHDA treatment, while MgSO(4) was without effect. The protective action of iron was neutralized by a membrane-permeable iron chelator o-phenanthroline, which also augmented astrocyte killing in the absence of exogenous iron. The mechanisms responsible for iron-mediated protection of astrocytes did not involve interference with either 6-OHDA autoxidation, hydrogen peroxide toxicity, or 6-OHDA-induced activation of extracellular signal-regulated kinase. Finally, the addition of iron potentiated and its chelation blocked 6-OHDA toxicity towards neuronal PC12 cells, suggesting the opposite roles for this transition metal in regulating the survival of astrocytes and dopaminergic neurons.
PB  - Elsevier Ltd.
T2  - Neuropharmacology
T1  - Iron protects astrocytes from 6-hydroxydopamine toxicity
IS  - 5
VL  - 48
DO  - 10.1016/j.neuropharm.2004.12.003
SP  - 720
EP  - 731
ER  - 

@article{
author = "Zogović, Nevena and Mladenović, Aleksandra and Perović, Milka and Harhaji-Trajković, Ljubica and Miljković, Đorđe and Trajković, Vladimir",
year = "2005",
abstract = "The role of iron in 6-hydroxydopamine (6-OHDA) toxicity towards astrocytes was investigated in vitro using rat primary astrocytes, rat astrocytoma cell line C6, and human astrocytoma cell line U251. The assessment of mitochondrial respiration or lactate dehydrogenase release has shown a dose-dependent decrease in the viability of astrocytes treated with 6-OHDA, which coincided with DNA fragmentation and the changes in cellular morphology. This was a consequence of the oxidative stress mediated by 6-OHDA autoxidation products hydrogen peroxide, superoxide anion, and hydroxyl radical. Both FeSO(4) and FeCl(3) markedly alleviated detrimental effects of 6-OHDA treatment, while MgSO(4) was without effect. The protective action of iron was neutralized by a membrane-permeable iron chelator o-phenanthroline, which also augmented astrocyte killing in the absence of exogenous iron. The mechanisms responsible for iron-mediated protection of astrocytes did not involve interference with either 6-OHDA autoxidation, hydrogen peroxide toxicity, or 6-OHDA-induced activation of extracellular signal-regulated kinase. Finally, the addition of iron potentiated and its chelation blocked 6-OHDA toxicity towards neuronal PC12 cells, suggesting the opposite roles for this transition metal in regulating the survival of astrocytes and dopaminergic neurons.",
publisher = "Elsevier Ltd.",
journal = "Neuropharmacology",
title = "Iron protects astrocytes from 6-hydroxydopamine toxicity",
number = "5",
volume = "48",
doi = "10.1016/j.neuropharm.2004.12.003",
pages = "720-731"
}

Zogović, N., Mladenović, A., Perović, M., Harhaji-Trajković, L., Miljković, Đ.,& Trajković, V.. (2005). Iron protects astrocytes from 6-hydroxydopamine toxicity. in Neuropharmacology
Elsevier Ltd.., 48(5), 720-731.
https://doi.org/10.1016/j.neuropharm.2004.12.003

Zogović N, Mladenović A, Perović M, Harhaji-Trajković L, Miljković Đ, Trajković V. Iron protects astrocytes from 6-hydroxydopamine toxicity. in Neuropharmacology. 2005;48(5):720-731.
doi:10.1016/j.neuropharm.2004.12.003 .

Zogović, Nevena, Mladenović, Aleksandra, Perović, Milka, Harhaji-Trajković, Ljubica, Miljković, Đorđe, Trajković, Vladimir, "Iron protects astrocytes from 6-hydroxydopamine toxicity" in Neuropharmacology, 48, no. 5 (2005):720-731,
https://doi.org/10.1016/j.neuropharm.2004.12.003 . .