@conference{
author = "Đorđević, Miloš and Grdović, Nevena and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Uskoković, Aleksandra and Rajić, Jovana and Đorđević, Marija and Tolić, Anja and Mišić, Danijela and Šiler, Branislav and Poznanović, Goran and Vidaković, Melita and Dinić, Svetlana",
year = "2019",
abstract = "Diabetes is characterized by hyperglycemia resulting from a deficiency in insulin secretion and/or action leading to serve diabetic complications. Despite numerous efforts, recovery, and maintenance of functional beta-cells is still an unresolved task in diabetes therapy. Considering anti-diabetic properties of medicinal herb Centaurium erythraea Rafn (CE), this study aimed to analyze protective effects of the CE extract on Rin-5F beta-cell line exposed to diabetogenic agent streptozotocin (STZ). Cytoprotective concentration of CE extract (0.25 mg/mL) and IC50 dose of STZ (12 mM) were determined using cell viability assay (MTT). The level of insulin mRNA and the concentration of insulin released from beta-cells in a culture medium were analyzed by RT-qPCR and ELISA, respectively. Activity of Akt, ERK and p38 kinases, as well as nuclear levels of islet-enriched Pdx1 and MafA proteins were assessed by Western blot analysis. In comparison to STZ-treated beta-cells, CE extract/STZ co-treatment increased viability of Rin-5F cells for 12%. STZ-treated beta-cells displayed reduction mRNA level of insulin to 63% and reduced insulin secretion to 76% in comparison to control, while application of CE extract improved insulin mRNA level to 77% and insulin secretion to 90% of the control level. Improved viability and functionality of beta-cells could be ascribed to a CE extract-mediated modulation of the activities of pro-survival Akt, ERK and p38 kinases and Pdx1 and MafA factors involved in regulation of beta-cell proliferation and insulin expression/secretion. The results of this study suggest that CE extract promotes proliferative and pro-survival pathways in beta-cells and improves their functional properties.",
publisher = "Lisboa: Universidade Lusófona",
journal = "NutRedOx COST Action CA 16112; 2019 Oct 2-4; Lisboa, Portugal",
title = "Centaurium erithraea extract mediates prosurvival pathways and insulin expression in STZ-treated beta-cells",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5692"
}