TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar J
AU  - Savić, Danijela
AU  - Šelih, Vid Simon
AU  - Živin, Marko
AU  - Sokić, Dragoslav
AU  - Raičević, Savo
AU  - Baščarević, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://xlink.rsc.org/?DOI=C6MT00293E
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28124054
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2583
AB  - The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 μg g(-1)) was found in mossy fiber-rich regions - cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (>10 μg g(-1)) and Mn (>15 μg g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 ± 0.91 μg g(-1)), compared to the subiculum (13.32 ± 1.29 μg g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p < 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.
T2  - Metallomics : integrated biometal science
T1  - Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.
IS  - 2
VL  - 9
DO  - 10.1039/c6mt00293e
SP  - 141
EP  - 148
ER  - 

@article{
author = "Opačić, Miloš and Ristić, Aleksandar J and Savić, Danijela and Šelih, Vid Simon and Živin, Marko and Sokić, Dragoslav and Raičević, Savo and Baščarević, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 μg g(-1)) was found in mossy fiber-rich regions - cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (>10 μg g(-1)) and Mn (>15 μg g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 ± 0.91 μg g(-1)), compared to the subiculum (13.32 ± 1.29 μg g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p < 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.",
journal = "Metallomics : integrated biometal science",
title = "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.",
number = "2",
volume = "9",
doi = "10.1039/c6mt00293e",
pages = "141-148"
}

Opačić, M., Ristić, A. J., Savić, D., Šelih, V. S., Živin, M., Sokić, D., Raičević, S., Baščarević, V.,& Spasojević, I.. (2017). Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.. in Metallomics : integrated biometal science, 9(2), 141-148.
https://doi.org/10.1039/c6mt00293e

Opačić M, Ristić AJ, Savić D, Šelih VS, Živin M, Sokić D, Raičević S, Baščarević V, Spasojević I. Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.. in Metallomics : integrated biometal science. 2017;9(2):141-148.
doi:10.1039/c6mt00293e .

Opačić, Miloš, Ristić, Aleksandar J, Savić, Danijela, Šelih, Vid Simon, Živin, Marko, Sokić, Dragoslav, Raičević, Savo, Baščarević, Vladimir, Spasojević, Ivan, "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy." in Metallomics : integrated biometal science, 9, no. 2 (2017):141-148,
https://doi.org/10.1039/c6mt00293e . .