TY  - CONF
AU  - Momčilović, Ivana
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4362
AB  - Protein synthesis elongation factors EF-Tu and eEF1A (EFs) are highly conserved and abundant GTPases in living cells, with a major role in transporting the aminoacyl-tRNA complex to the ribosome during translation. EFs are also considered multifunctional proteins with numerous noncanonical activities. EFs possess chaperone activity in preventing protein aggregation, interact with misfolded newly synthesized polypeptides and possibly direct them to the proteasome, and participate in viral replication. eEF1A is also implicated in the organization of cytoskeleton and apoptosis, while EF-Tu of bacterial pathogens elicits plant innate immunity. There is growing evidence that up-regulation of EFs genes by abiotic stresses may play an important role in stress responses in plants. The heat stress-induced accumulation of EFs has been detected in a number of plant species, and it correlates with the heat tolerant phenotype in maize, wheat, and potato. Accumulation of EFs may enhance protein synthesis and the capability of plant cells to sustain heat stress. Besides, the proposed EFs role in the quality control of newly synthesized proteins and chaperone activity in preventing protein thermal aggregation and inactivation might be important in alleviating negative effects of high temperature. Taken together, these multifunctional proteins may represent coordinators of the cellular processes in stressed plants, for instance, protein synthesis with recognition and repair/degradation of misfolded proteins.
PB  - Belgrade: Faculty of Chemistry
C3  - Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings
T1  - Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4362
ER  - 

@conference{
author = "Momčilović, Ivana",
year = "2019",
abstract = "Protein synthesis elongation factors EF-Tu and eEF1A (EFs) are highly conserved and abundant GTPases in living cells, with a major role in transporting the aminoacyl-tRNA complex to the ribosome during translation. EFs are also considered multifunctional proteins with numerous noncanonical activities. EFs possess chaperone activity in preventing protein aggregation, interact with misfolded newly synthesized polypeptides and possibly direct them to the proteasome, and participate in viral replication. eEF1A is also implicated in the organization of cytoskeleton and apoptosis, while EF-Tu of bacterial pathogens elicits plant innate immunity. There is growing evidence that up-regulation of EFs genes by abiotic stresses may play an important role in stress responses in plants. The heat stress-induced accumulation of EFs has been detected in a number of plant species, and it correlates with the heat tolerant phenotype in maize, wheat, and potato. Accumulation of EFs may enhance protein synthesis and the capability of plant cells to sustain heat stress. Besides, the proposed EFs role in the quality control of newly synthesized proteins and chaperone activity in preventing protein thermal aggregation and inactivation might be important in alleviating negative effects of high temperature. Taken together, these multifunctional proteins may represent coordinators of the cellular processes in stressed plants, for instance, protein synthesis with recognition and repair/degradation of misfolded proteins.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings",
title = "Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4362"
}

Momčilović, I.. (2019). Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance. in Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings
Belgrade: Faculty of Chemistry., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_4362

Momčilović I. Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance. in Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings. 2019;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_4362 .

Momčilović, Ivana, "Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance" in Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings (2019):41,
https://hdl.handle.net/21.15107/rcub_ibiss_4362 .

TY  - THES
AU  - Pantelić, Danijel
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3259
AB  - Eukariotski elongacioni faktor translacije 1A (eEF1A) je citosolni, multifunkcionalni protein koji ima ključnu ulogu u elongacionoj fazi tokom sinteze proteina, vršeći transport aa-tRNK do mesta A na ribozomima. Pored osnovne funkcije, ovaj elongacioni faktor može učestvovati i u eksportu molekula iz nukleusa, organizaciji citoskeleta, proteolitičkoj degradaciji proteina, kao i posedovati aktivnost molekularnog šaperona. U ovoj tezi ispitan je uticaj visoke temperature na proces tuberizacije, rastenje i razviće, kao i ekspresiju eEF1A u izdancima, listovima i krtolama kod različitih sorti krompira gajenih u kontrolisanim uslovima ili u polju. Pored toga, urađena je i karakterizacija izoformi eEF1A u listovima kod različitih sorti krompira gajenih ex vitro ili u polju. Kod različitih sorti krompira izvršena je procena broja kopija gena koji kodiraju eEF1A, a ekspresija eEF1A gena u uslovima visoke temperature je ispitana kod sorti Désirée i Festival. Analiza zastupljenost eEF1A u mikro- i minikrtolama kod toplotno-osetljive sorte Désirée, je pokazala da u uslovima dugotrajnog toplotnog stresa dolazi do smanjenja zastupljenosti eEF1A, dok sa druge strane kod toplotno-tolerantne sorte Festival, dolazi do povećanja zastupljenosti eEF1A, kako u mikrokrtolama, tako i u starijim minikrtolama. Ovaj rezultat ukazuje na ulogu eEF1A u ublažavanju negativnog uticaja visoke temperature na proces tuberizacije kod krompira. Značajna korelacija ustanovljena je između zastupljenosti eEF1A u listovima krompira gajenih u polju tokom dve izuzetno tople godine (2011. i 2012.) i prinosa krompira. Rezultati eksperimenata u uslovima ex vitro, kao i polju, su pokazali da je relativna molekulska masa eEF1A krompira 49,2 kDa i da ima 5-8 izoformi. Povećanoj zastupljenosti eEF1A u uslovima visoke temperature, najviše su doprinela 2-3 polipeptida/izoforme iz bazne grupe. Genomska analiza je ukazala na postojanje više gena koji kodiraju eEF1A kod krompira, kao i da se ispitivane sorte razlikuju po broju kopija gena. Analiza ekspresije eEF1A gena u minikrtolama toplotno-osetljive sorte Désirée pokazala da je ekspresija većine gena osetljiva na delovanje visoke temperature, te dolazi do značajnog smanjenjenja broja većine eEF1A transkripata u uslovima toplotnog stresa. Sa druge strane, kod toplotno-tolerantne sorte Festival, visoka temperatura je imala blaži efekat na ekspresiju većine eEF1A gena, odnosno zastupljenost pojedinih transkripata se blago povećala ili ostala na nivou kontrole u uslovima visoke temperature. Izuzetak je predstavljao transkript eEF1A-12 gena koji se karakterisao izuzetnom inducibilnošću. Na osnovu dobijenih rezultata može se zaključiti da ekspresija gena eEF1A-12 u uslovima visoke temperature najviše doprinosi povećanju zastupljenosti eEF1A proteina u krtolama toplotno-tolerantne sorte Festival, što otvara mogućnost za njegovo korišćenje u kreiranju novih, termotolerantnih sorti krompira putem klasičnih metoda selekcije ili metoda genetičkog inženjeringa.
AB  - Eukaryotic elongation factor 1A (eEF1A) is a cytosolic, multifunctional protein that plays a central role in the elongation phase of translation by delivering aa-tRNA complex to the A site of the ribosome. The eEF1A is also implicated in the nuclear export of aa-tRNA, organization of the cytoskeleton, participates in proteolysis, and possess chaperone activity. In this thesis, the effects of high temperatures on tuberization, growth, and development, as well as the expression and accumulation of eEF1A in shoots, leaves, and tubers, were investigated in different potato cultivars grown in a controlled environment and field. In addition, the characterization of eEF1A isoforms in leaves of different potato cultivars grown ex vitro or in the field was done. Also, in different potato cultivars, the number of eEF1A-coding genes, as well as the eEF1A genes' transcript expression under a high-temperature treatment, were investigated. Investigation of eEF1A accumulation in micro- and minitubers of heat-sensitive cultivar Désirée revealed a lower level of eEF1A under prolonged heat-stress, while in heat-tolerant cultivar Festival, a higher level of eEF1A in microtubers and older minitubers was found. Also, a significant positive correlation between eEF1A abundance in potato leaves and the potato productivity in the field was observed in two extremely hot years 2001. and 2012. These results indicate an important eEF1A role in mitigation of the adverse impacts of high temperature on the tuberization in potato. Results from ex vitro and field experiments have shown that potato eEF1A is a protein of 49.2 kDa with multiple isoforms (5-8). Increase in eEF1A abundance under high-temperature condition was mainly due to 2-3 basic polypeptides/isoforms. Genomic analysis indicated the existence of multiple genes encoding eEF1A in potato. Analysis of eEF1A genes' transcripts in minitubers of heat-sensitive cultivar Désirée revealed that expression of the most genes was sensitive to high temperature, and there was a significant reduction in number of eEF1A transcripts. On the other hand, in heat-tolerant cultivar Festival, high temperature had a milder effect on eEF1A gene-expression. The abundance of most eEF1A transcripts slightly increased or remained unchanged under high-temperature condition, with the exception of the eEF1A-12 transcript which was highly heat-inducible. Based on results obtained in this thesis, it can be concluded that eEF1A-12 gene expression mainly contributes to an observed increase in eEF1A abundance under high-temperature conditions in tubers of heat-tolerant cultivar Festival, which opens the possibility for its utilization in the development of new heat-tolerant potato varieties by classical breeding or genetic engineering.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres
T1  - Translation elongation factor 1A in potato (Solanum tuberosum L.): characterisation of isoforms, expression, and role in plant response to heat-stress
SP  - 1
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3259
ER  - 

@phdthesis{
author = "Pantelić, Danijel",
year = "2019",
abstract = "Eukariotski elongacioni faktor translacije 1A (eEF1A) je citosolni, multifunkcionalni protein koji ima ključnu ulogu u elongacionoj fazi tokom sinteze proteina, vršeći transport aa-tRNK do mesta A na ribozomima. Pored osnovne funkcije, ovaj elongacioni faktor može učestvovati i u eksportu molekula iz nukleusa, organizaciji citoskeleta, proteolitičkoj degradaciji proteina, kao i posedovati aktivnost molekularnog šaperona. U ovoj tezi ispitan je uticaj visoke temperature na proces tuberizacije, rastenje i razviće, kao i ekspresiju eEF1A u izdancima, listovima i krtolama kod različitih sorti krompira gajenih u kontrolisanim uslovima ili u polju. Pored toga, urađena je i karakterizacija izoformi eEF1A u listovima kod različitih sorti krompira gajenih ex vitro ili u polju. Kod različitih sorti krompira izvršena je procena broja kopija gena koji kodiraju eEF1A, a ekspresija eEF1A gena u uslovima visoke temperature je ispitana kod sorti Désirée i Festival. Analiza zastupljenost eEF1A u mikro- i minikrtolama kod toplotno-osetljive sorte Désirée, je pokazala da u uslovima dugotrajnog toplotnog stresa dolazi do smanjenja zastupljenosti eEF1A, dok sa druge strane kod toplotno-tolerantne sorte Festival, dolazi do povećanja zastupljenosti eEF1A, kako u mikrokrtolama, tako i u starijim minikrtolama. Ovaj rezultat ukazuje na ulogu eEF1A u ublažavanju negativnog uticaja visoke temperature na proces tuberizacije kod krompira. Značajna korelacija ustanovljena je između zastupljenosti eEF1A u listovima krompira gajenih u polju tokom dve izuzetno tople godine (2011. i 2012.) i prinosa krompira. Rezultati eksperimenata u uslovima ex vitro, kao i polju, su pokazali da je relativna molekulska masa eEF1A krompira 49,2 kDa i da ima 5-8 izoformi. Povećanoj zastupljenosti eEF1A u uslovima visoke temperature, najviše su doprinela 2-3 polipeptida/izoforme iz bazne grupe. Genomska analiza je ukazala na postojanje više gena koji kodiraju eEF1A kod krompira, kao i da se ispitivane sorte razlikuju po broju kopija gena. Analiza ekspresije eEF1A gena u minikrtolama toplotno-osetljive sorte Désirée pokazala da je ekspresija većine gena osetljiva na delovanje visoke temperature, te dolazi do značajnog smanjenjenja broja većine eEF1A transkripata u uslovima toplotnog stresa. Sa druge strane, kod toplotno-tolerantne sorte Festival, visoka temperatura je imala blaži efekat na ekspresiju većine eEF1A gena, odnosno zastupljenost pojedinih transkripata se blago povećala ili ostala na nivou kontrole u uslovima visoke temperature. Izuzetak je predstavljao transkript eEF1A-12 gena koji se karakterisao izuzetnom inducibilnošću. Na osnovu dobijenih rezultata može se zaključiti da ekspresija gena eEF1A-12 u uslovima visoke temperature najviše doprinosi povećanju zastupljenosti eEF1A proteina u krtolama toplotno-tolerantne sorte Festival, što otvara mogućnost za njegovo korišćenje u kreiranju novih, termotolerantnih sorti krompira putem klasičnih metoda selekcije ili metoda genetičkog inženjeringa., Eukaryotic elongation factor 1A (eEF1A) is a cytosolic, multifunctional protein that plays a central role in the elongation phase of translation by delivering aa-tRNA complex to the A site of the ribosome. The eEF1A is also implicated in the nuclear export of aa-tRNA, organization of the cytoskeleton, participates in proteolysis, and possess chaperone activity. In this thesis, the effects of high temperatures on tuberization, growth, and development, as well as the expression and accumulation of eEF1A in shoots, leaves, and tubers, were investigated in different potato cultivars grown in a controlled environment and field. In addition, the characterization of eEF1A isoforms in leaves of different potato cultivars grown ex vitro or in the field was done. Also, in different potato cultivars, the number of eEF1A-coding genes, as well as the eEF1A genes' transcript expression under a high-temperature treatment, were investigated. Investigation of eEF1A accumulation in micro- and minitubers of heat-sensitive cultivar Désirée revealed a lower level of eEF1A under prolonged heat-stress, while in heat-tolerant cultivar Festival, a higher level of eEF1A in microtubers and older minitubers was found. Also, a significant positive correlation between eEF1A abundance in potato leaves and the potato productivity in the field was observed in two extremely hot years 2001. and 2012. These results indicate an important eEF1A role in mitigation of the adverse impacts of high temperature on the tuberization in potato. Results from ex vitro and field experiments have shown that potato eEF1A is a protein of 49.2 kDa with multiple isoforms (5-8). Increase in eEF1A abundance under high-temperature condition was mainly due to 2-3 basic polypeptides/isoforms. Genomic analysis indicated the existence of multiple genes encoding eEF1A in potato. Analysis of eEF1A genes' transcripts in minitubers of heat-sensitive cultivar Désirée revealed that expression of the most genes was sensitive to high temperature, and there was a significant reduction in number of eEF1A transcripts. On the other hand, in heat-tolerant cultivar Festival, high temperature had a milder effect on eEF1A gene-expression. The abundance of most eEF1A transcripts slightly increased or remained unchanged under high-temperature condition, with the exception of the eEF1A-12 transcript which was highly heat-inducible. Based on results obtained in this thesis, it can be concluded that eEF1A-12 gene expression mainly contributes to an observed increase in eEF1A abundance under high-temperature conditions in tubers of heat-tolerant cultivar Festival, which opens the possibility for its utilization in the development of new heat-tolerant potato varieties by classical breeding or genetic engineering.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres, Translation elongation factor 1A in potato (Solanum tuberosum L.): characterisation of isoforms, expression, and role in plant response to heat-stress",
pages = "1-142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3259"
}

Pantelić, D.. (2019). Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-142.
https://hdl.handle.net/21.15107/rcub_ibiss_3259

Pantelić D. Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres. in University of Belgrade, Faculty of Biology. 2019;:1-142.
https://hdl.handle.net/21.15107/rcub_ibiss_3259 .

Pantelić, Danijel, "Elongacioni faktor translacije 1A kod krompira (Solanum tuberosum L.): karakterizacija izoformi, ekspresija i uloga u odgovoru biljaka na toplotni stres" in University of Belgrade, Faculty of Biology (2019):1-142,
https://hdl.handle.net/21.15107/rcub_ibiss_3259 .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Oljača, Jasmina
AU  - Simonović, Ana
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4348
AB  - Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance
SP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4348
ER  - 

@conference{
editor = "Kovačević, Dušan",
author = "Pantelić, Danijel and Rudić, Jelena and Oljača, Jasmina and Simonović, Ana and Broćić, Zoran and Momčilović, Ivana",
year = "2018",
abstract = "Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance",
pages = "335",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4348"
}

Kovačević, D., Pantelić, D., Rudić, J., Oljača, J., Simonović, A., Broćić, Z.,& Momčilović, I.. (2018). Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348

Kovačević D, Pantelić D, Rudić J, Oljača J, Simonović A, Broćić Z, Momčilović I. Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2018;:335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

Kovačević, Dušan, Pantelić, Danijel, Rudić, Jelena, Oljača, Jasmina, Simonović, Ana, Broćić, Zoran, Momčilović, Ivana, "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance" in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina (2018):335,
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

TY  - CONF
AU  - Oljača, Jasmina
AU  - Broćič, Zoran
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4349
AB  - Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - IX International Scientific Agriculture Symposium “Agrosym 2018”
T1  - Prospective protein markers for selection of heat tolerant potato cultivars
SP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4349
ER  - 

@conference{
author = "Oljača, Jasmina and Broćič, Zoran and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2018",
abstract = "Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "IX International Scientific Agriculture Symposium “Agrosym 2018”",
title = "Prospective protein markers for selection of heat tolerant potato cultivars",
pages = "340",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4349"
}

Oljača, J., Broćič, Z., Pantelić, D., Rudić, J.,& Momčilović, I.. (2018). Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349

Oljača J, Broćič Z, Pantelić D, Rudić J, Momčilović I. Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”. 2018;:340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

Oljača, Jasmina, Broćič, Zoran, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Prospective protein markers for selection of heat tolerant potato cultivars" in IX International Scientific Agriculture Symposium “Agrosym 2018” (2018):340,
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

TY  - JOUR
AU  - Pantelić, Danijel
AU  - Dragićević, Ivana Č.
AU  - Rudić, Jelena
AU  - Fu, Jianming
AU  - Momčilović, Ivana
PY  - 2018
UR  - http://link.springer.com/10.1007/s13580-018-0043-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3089
AB  - Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.
T2  - Horticulture, Environment, and Biotechnology
T1  - Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato
IS  - 3
VL  - 59
DO  - 10.1007/s13580-018-0043-x
SP  - 315
EP  - 324
ER  - 

@article{
author = "Pantelić, Danijel and Dragićević, Ivana Č. and Rudić, Jelena and Fu, Jianming and Momčilović, Ivana",
year = "2018",
abstract = "Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.",
journal = "Horticulture, Environment, and Biotechnology",
title = "Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato",
number = "3",
volume = "59",
doi = "10.1007/s13580-018-0043-x",
pages = "315-324"
}

Pantelić, D., Dragićević, I. Č., Rudić, J., Fu, J.,& Momčilović, I.. (2018). Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. in Horticulture, Environment, and Biotechnology, 59(3), 315-324.
https://doi.org/10.1007/s13580-018-0043-x

Pantelić D, Dragićević IČ, Rudić J, Fu J, Momčilović I. Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. in Horticulture, Environment, and Biotechnology. 2018;59(3):315-324.
doi:10.1007/s13580-018-0043-x .

Pantelić, Danijel, Dragićević, Ivana Č., Rudić, Jelena, Fu, Jianming, Momčilović, Ivana, "Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato" in Horticulture, Environment, and Biotechnology, 59, no. 3 (2018):315-324,
https://doi.org/10.1007/s13580-018-0043-x . .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Pantelić, Danijel
AU  - Simonović, Ana
AU  - Trajković, Milena
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4337
AB  - Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4337
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Pantelić, Danijel and Simonović, Ana and Trajković, Milena and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4337"
}

Antonić, D., Milošević, S., Pantelić, D., Simonović, A., Trajković, M., Momčilović, I.,& Subotić, A.. (2018). Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337

Antonić D, Milošević S, Pantelić D, Simonović A, Trajković M, Momčilović I, Subotić A. Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

Antonić, Dragana, Milošević, Snežana, Pantelić, Danijel, Simonović, Ana, Trajković, Milena, Momčilović, Ivana, Subotić, Angelina, "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

TY  - CONF
AU  - Trailović, Maja
AU  - Dragićević, Ivana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4321
AB  - Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK). SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija
T1  - Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro
SP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4321
ER  - 

@conference{
author = "Trailović, Maja and Dragićević, Ivana and Raspor, Martin and Motyka, Vaclav and Ninković, Slavica",
year = "2018",
abstract = "Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK). SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija",
title = "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro",
pages = "62",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4321"
}

Trailović, M., Dragićević, I., Raspor, M., Motyka, V.,& Ninković, S.. (2018). Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro. in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija
Belgrade: Serbian Biological Society., 62.
https://hdl.handle.net/21.15107/rcub_ibiss_4321

Trailović M, Dragićević I, Raspor M, Motyka V, Ninković S. Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro. in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija. 2018;:62.
https://hdl.handle.net/21.15107/rcub_ibiss_4321 .

Trailović, Maja, Dragićević, Ivana, Raspor, Martin, Motyka, Vaclav, Ninković, Slavica, "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro" in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija (2018):62,
https://hdl.handle.net/21.15107/rcub_ibiss_4321 .