TY  - DATA
AU  - Jakubek, Patrycja
AU  - Rajić, Jovana
AU  - Kuczynska, Monika
AU  - Suliborska, Klaudia
AU  - Heldt, Mateusz
AU  - Dziedziul, Karol
AU  - Vidaković, Melita
AU  - Namiesnik, Jacek
AU  - Bartoszek, Agnieszka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5670
AB  - Table S1. Primer sequences for SRXN1 gene used in MSP and MS-HRM. Table S2. Primer sequences for SRXN1 and GAPDH genes used in RT-qPCR. Table S3. Methylation changes in CpG island within the promoter region of SRXN1 in HT29 cell line investigated with MSP. Cells were treated with catechins and glutathione at concentrations of 0.1, 1,10, and 100 µM for 24 h at 37°C. The results are means ± SD of three biological replicates. Table S4. Methylation changes in CpG island within the promoter region of SRXN1 in HT29 cell line investigated with MS-HRM. Cells were treated with catechins and glutathione at concentrations of 0.1,1, 10, and 100 µM for 24 h at 37°C. The results are means ± SD of three biological replicates. Table S5. Changes in SRXN1 gene expression analysed with the RT-qPCR method. Expression levels were calculated using relative delta delta Ct method. Data was normalized to GAPDH gene expression. The results are means ± SD of three biological replicates. Figure S1. Inhibition of growth of HT29 cells determined by MTT assay after 72 h treatment with 5-Aza. Viability is expressed as a percentage relative to control cells (100%). EC30 and EC45 are the effective concentrations of 30% and 45% of cell growth inhibition. Results are means of three independent experiments carried out in triplicate (SD < 15%). Figure S2. The increase in DNA methylation level of SRXN1 gene determined by MS-HRM using M1/U1 primer sets following 24 h treatment of HT29 cells with catechins that increase methylation
profile of SRXN1 promoter. Figure S3. The increase in DNA methylation level of SRXN1 gene determined by MS-HRM using M3/U3 primer sets following 24 h treatment of HT29 cells with catechins that increase methylation profile of SRXN1 promoter.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Supplementary material for the article:Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene
IS  - 3
VL  - 12
SP  - 754
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5670
ER  - 

@misc{
author = "Jakubek, Patrycja and Rajić, Jovana and Kuczynska, Monika and Suliborska, Klaudia and Heldt, Mateusz and Dziedziul, Karol and Vidaković, Melita and Namiesnik, Jacek and Bartoszek, Agnieszka",
year = "2023",
abstract = "Table S1. Primer sequences for SRXN1 gene used in MSP and MS-HRM. Table S2. Primer sequences for SRXN1 and GAPDH genes used in RT-qPCR. Table S3. Methylation changes in CpG island within the promoter region of SRXN1 in HT29 cell line investigated with MSP. Cells were treated with catechins and glutathione at concentrations of 0.1, 1,10, and 100 µM for 24 h at 37°C. The results are means ± SD of three biological replicates. Table S4. Methylation changes in CpG island within the promoter region of SRXN1 in HT29 cell line investigated with MS-HRM. Cells were treated with catechins and glutathione at concentrations of 0.1,1, 10, and 100 µM for 24 h at 37°C. The results are means ± SD of three biological replicates. Table S5. Changes in SRXN1 gene expression analysed with the RT-qPCR method. Expression levels were calculated using relative delta delta Ct method. Data was normalized to GAPDH gene expression. The results are means ± SD of three biological replicates. Figure S1. Inhibition of growth of HT29 cells determined by MTT assay after 72 h treatment with 5-Aza. Viability is expressed as a percentage relative to control cells (100%). EC30 and EC45 are the effective concentrations of 30% and 45% of cell growth inhibition. Results are means of three independent experiments carried out in triplicate (SD < 15%). Figure S2. The increase in DNA methylation level of SRXN1 gene determined by MS-HRM using M1/U1 primer sets following 24 h treatment of HT29 cells with catechins that increase methylation
profile of SRXN1 promoter. Figure S3. The increase in DNA methylation level of SRXN1 gene determined by MS-HRM using M3/U3 primer sets following 24 h treatment of HT29 cells with catechins that increase methylation profile of SRXN1 promoter.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Supplementary material for the article:Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene",
number = "3",
volume = "12",
pages = "754",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5670"
}

Jakubek, P., Rajić, J., Kuczynska, M., Suliborska, K., Heldt, M., Dziedziul, K., Vidaković, M., Namiesnik, J.,& Bartoszek, A.. (2023). Supplementary material for the article:Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene. in Antioxidants
Basel: MDPI., 12(3), 754.
https://hdl.handle.net/21.15107/rcub_ibiss_5670

Jakubek P, Rajić J, Kuczynska M, Suliborska K, Heldt M, Dziedziul K, Vidaković M, Namiesnik J, Bartoszek A. Supplementary material for the article:Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene. in Antioxidants. 2023;12(3):754.
https://hdl.handle.net/21.15107/rcub_ibiss_5670 .

Jakubek, Patrycja, Rajić, Jovana, Kuczynska, Monika, Suliborska, Klaudia, Heldt, Mateusz, Dziedziul, Karol, Vidaković, Melita, Namiesnik, Jacek, Bartoszek, Agnieszka, "Supplementary material for the article:Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene" in Antioxidants, 12, no. 3 (2023):754,
https://hdl.handle.net/21.15107/rcub_ibiss_5670 .

TY  - JOUR
AU  - Jakubek, Patrycja
AU  - Rajić, Jovana
AU  - Kuczynska, Monika
AU  - Suliborska, Klaudia
AU  - Heldt, Mateusz
AU  - Dziedziul, Karol
AU  - Vidaković, Melita
AU  - Namiesnik, Jacek
AU  - Bartoszek, Agnieszka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5506
AB  - The role of catechins in the epigenetic regulation of gene expression has been widely studied; however, if and how this phenomenon relates to the redox properties of these polyphenols remains unknown. Our earlier study demonstrated that exposure of the human colon adenocarcinoma HT29 cell line to these antioxidants affects the expression of redox-related genes. In particular, treatment with (−)-epigallocatechin (EGC) downregulated transcription of gene encoding sulfiredoxin-1 (SRXN1), the peroxidase involved in the protection of cells against hydrogen peroxide-induced oxidative stress. The aim of this study was to investigate whether the observed SRXN1 downregulation was accompanied by changes in the DNA methylation level of its promoter and, if so, whether it was correlated with the redox properties of catechins. The impact on DNA methylation profile in HT29 cells treated with different concentrations of five catechins, varying in chemical structures and standard reduction potentials as well as susceptibility to oxidation, was monitored by a methylation-sensitive high-resolution melting technique employing the SRXN1 promoter region as a model target. We demonstrated that catechins, indeed, are able to modulate DNA methylation of the SRXN1 gene in a redox-related manner. The nonlinear method in the statistical analysis made it possible to fish out two parameters (charge transfer in oxidation process Qox and time of electron transfer t), whose strong interactions correlated with observed modulation of DNA methylation by catechins. Based on these findings, we present a proof-of-concept that DNA methylation, which limits SRXN1 expression and thus restricts the multidirectional antioxidant action of SRXN1, may represent a mechanism protecting cells against reductive stress caused by particularly fast-reacting reductants such as EGC and (−)-epicatechin gallate (ECG) in our study.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene
IS  - 3
VL  - 12
DO  - 10.3390/antiox12030754
SP  - 754
ER  - 

@article{
author = "Jakubek, Patrycja and Rajić, Jovana and Kuczynska, Monika and Suliborska, Klaudia and Heldt, Mateusz and Dziedziul, Karol and Vidaković, Melita and Namiesnik, Jacek and Bartoszek, Agnieszka",
year = "2023",
abstract = "The role of catechins in the epigenetic regulation of gene expression has been widely studied; however, if and how this phenomenon relates to the redox properties of these polyphenols remains unknown. Our earlier study demonstrated that exposure of the human colon adenocarcinoma HT29 cell line to these antioxidants affects the expression of redox-related genes. In particular, treatment with (−)-epigallocatechin (EGC) downregulated transcription of gene encoding sulfiredoxin-1 (SRXN1), the peroxidase involved in the protection of cells against hydrogen peroxide-induced oxidative stress. The aim of this study was to investigate whether the observed SRXN1 downregulation was accompanied by changes in the DNA methylation level of its promoter and, if so, whether it was correlated with the redox properties of catechins. The impact on DNA methylation profile in HT29 cells treated with different concentrations of five catechins, varying in chemical structures and standard reduction potentials as well as susceptibility to oxidation, was monitored by a methylation-sensitive high-resolution melting technique employing the SRXN1 promoter region as a model target. We demonstrated that catechins, indeed, are able to modulate DNA methylation of the SRXN1 gene in a redox-related manner. The nonlinear method in the statistical analysis made it possible to fish out two parameters (charge transfer in oxidation process Qox and time of electron transfer t), whose strong interactions correlated with observed modulation of DNA methylation by catechins. Based on these findings, we present a proof-of-concept that DNA methylation, which limits SRXN1 expression and thus restricts the multidirectional antioxidant action of SRXN1, may represent a mechanism protecting cells against reductive stress caused by particularly fast-reacting reductants such as EGC and (−)-epicatechin gallate (ECG) in our study.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene",
number = "3",
volume = "12",
doi = "10.3390/antiox12030754",
pages = "754"
}

Jakubek, P., Rajić, J., Kuczynska, M., Suliborska, K., Heldt, M., Dziedziul, K., Vidaković, M., Namiesnik, J.,& Bartoszek, A.. (2023). Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene. in Antioxidants
Basel: MDPI., 12(3), 754.
https://doi.org/10.3390/antiox12030754

Jakubek P, Rajić J, Kuczynska M, Suliborska K, Heldt M, Dziedziul K, Vidaković M, Namiesnik J, Bartoszek A. Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene. in Antioxidants. 2023;12(3):754.
doi:10.3390/antiox12030754 .

Jakubek, Patrycja, Rajić, Jovana, Kuczynska, Monika, Suliborska, Klaudia, Heldt, Mateusz, Dziedziul, Karol, Vidaković, Melita, Namiesnik, Jacek, Bartoszek, Agnieszka, "Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene" in Antioxidants, 12, no. 3 (2023):754,
https://doi.org/10.3390/antiox12030754 . .