TY  - JOUR
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3603
AB  - In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethno-medicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L. sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 μg/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher anti-angiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong antimigratory effect and selective endothelial cytotoxicity in relation to lung fibroblasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.
T2  - International Journal of Biological Macromolecules
T1  - Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.
VL  - 148
DO  - 10.1016/j.ijbiomac.2020.01.033
SP  - 129
EP  - 139
ER  - 

@article{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethno-medicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L. sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 μg/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher anti-angiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong antimigratory effect and selective endothelial cytotoxicity in relation to lung fibroblasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.",
journal = "International Journal of Biological Macromolecules",
title = "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.",
volume = "148",
doi = "10.1016/j.ijbiomac.2020.01.033",
pages = "129-139"
}

Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in International Journal of Biological Macromolecules, 148, 129-139.
https://doi.org/10.1016/j.ijbiomac.2020.01.033

Petrović J, Glamočlija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in International Journal of Biological Macromolecules. 2020;148:129-139.
doi:10.1016/j.ijbiomac.2020.01.033 .

Petrović, Jovana, Glamočlija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma." in International Journal of Biological Macromolecules, 148 (2020):129-139,
https://doi.org/10.1016/j.ijbiomac.2020.01.033 . .

TY  - JOUR
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Vojnović, Sandra
AU  - Ćirić, Ana
AU  - Veljić, Milan
AU  - Nikodinović-Runić, Jasmina
AU  - Novaković, Miroslav
PY  - 2017
UR  - http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-107674
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2686
AB  - Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40–70?%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was shown to significantly decrease the production of acyl homoserine lactones (AHL) in P. aeruginosa PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)-l-HSL. On the other side, four diarylheptanoids (2–5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the P. aeruginosa quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their in vitro antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 µg/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.
T2  - Planta Medica
T1  - Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa
IS  - 01/02
VL  - 83
DO  - 10.1055/s-0042-107674
SP  - 117
EP  - 125
ER  - 

@article{
author = "Ilić-Tomić, Tatjana and Soković, Marina and Vojnović, Sandra and Ćirić, Ana and Veljić, Milan and Nikodinović-Runić, Jasmina and Novaković, Miroslav",
year = "2017",
abstract = "Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40–70?%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was shown to significantly decrease the production of acyl homoserine lactones (AHL) in P. aeruginosa PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)-l-HSL. On the other side, four diarylheptanoids (2–5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the P. aeruginosa quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their in vitro antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 µg/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.",
journal = "Planta Medica",
title = "Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa",
number = "01/02",
volume = "83",
doi = "10.1055/s-0042-107674",
pages = "117-125"
}

Ilić-Tomić, T., Soković, M., Vojnović, S., Ćirić, A., Veljić, M., Nikodinović-Runić, J.,& Novaković, M.. (2017). Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa. in Planta Medica, 83(01/02), 117-125.
https://doi.org/10.1055/s-0042-107674

Ilić-Tomić T, Soković M, Vojnović S, Ćirić A, Veljić M, Nikodinović-Runić J, Novaković M. Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa. in Planta Medica. 2017;83(01/02):117-125.
doi:10.1055/s-0042-107674 .

Ilić-Tomić, Tatjana, Soković, Marina, Vojnović, Sandra, Ćirić, Ana, Veljić, Milan, Nikodinović-Runić, Jasmina, Novaković, Miroslav, "Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa" in Planta Medica, 83, no. 01/02 (2017):117-125,
https://doi.org/10.1055/s-0042-107674 . .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Nikodinović-Runić, Jasmina
AU  - Veselinović, Jovana
AU  - Ilić-Tomić, Tatjana
AU  - Vidaković, Vera
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2017
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.jnatprod.6b00805
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2765
AB  - Seven derivatives of pentacyclic triterpene acids (1-7) were isolated from the bark of Alnus viridis ssp. viridis using a combination of column chromatography and semipreparative HPLC. Compounds 1-3, 6, and 7 were determined to be new after spectroscopic data interpretation and were assigned as 27-hydroxyalphitolic acid derivatives (1-3), a 27-hydroxybetulinic acid derivative (6), and a 3-epi-maslinic acid derivative (7), respectively. Pentacyclic triterpenoids with a C-27 hydroxymethyl group have been found in species of the genus Alnus for the first time. These compounds were subjected to cytotoxicity testing against a number of cancer cell lines. Also, selected pentacyclic triterpenoids were selected as potential inhibitors of topoisomerases I and IIα for an in silico investigation.
T2  - Journal of Natural Products
T1  - Bioactive Pentacyclic Triterpene Ester Derivatives from Alnus viridis ssp. viridis Bark
IS  - 5
VL  - 80
DO  - 10.1021/acs.jnatprod.6b00805
SP  - 1255
EP  - 1263
ER  - 

@article{
author = "Novaković, Miroslav and Nikodinović-Runić, Jasmina and Veselinović, Jovana and Ilić-Tomić, Tatjana and Vidaković, Vera and Tešević, Vele and Milosavljević, Slobodan",
year = "2017",
abstract = "Seven derivatives of pentacyclic triterpene acids (1-7) were isolated from the bark of Alnus viridis ssp. viridis using a combination of column chromatography and semipreparative HPLC. Compounds 1-3, 6, and 7 were determined to be new after spectroscopic data interpretation and were assigned as 27-hydroxyalphitolic acid derivatives (1-3), a 27-hydroxybetulinic acid derivative (6), and a 3-epi-maslinic acid derivative (7), respectively. Pentacyclic triterpenoids with a C-27 hydroxymethyl group have been found in species of the genus Alnus for the first time. These compounds were subjected to cytotoxicity testing against a number of cancer cell lines. Also, selected pentacyclic triterpenoids were selected as potential inhibitors of topoisomerases I and IIα for an in silico investigation.",
journal = "Journal of Natural Products",
title = "Bioactive Pentacyclic Triterpene Ester Derivatives from Alnus viridis ssp. viridis Bark",
number = "5",
volume = "80",
doi = "10.1021/acs.jnatprod.6b00805",
pages = "1255-1263"
}

Novaković, M., Nikodinović-Runić, J., Veselinović, J., Ilić-Tomić, T., Vidaković, V., Tešević, V.,& Milosavljević, S.. (2017). Bioactive Pentacyclic Triterpene Ester Derivatives from Alnus viridis ssp. viridis Bark. in Journal of Natural Products, 80(5), 1255-1263.
https://doi.org/10.1021/acs.jnatprod.6b00805

Novaković M, Nikodinović-Runić J, Veselinović J, Ilić-Tomić T, Vidaković V, Tešević V, Milosavljević S. Bioactive Pentacyclic Triterpene Ester Derivatives from Alnus viridis ssp. viridis Bark. in Journal of Natural Products. 2017;80(5):1255-1263.
doi:10.1021/acs.jnatprod.6b00805 .

Novaković, Miroslav, Nikodinović-Runić, Jasmina, Veselinović, Jovana, Ilić-Tomić, Tatjana, Vidaković, Vera, Tešević, Vele, Milosavljević, Slobodan, "Bioactive Pentacyclic Triterpene Ester Derivatives from Alnus viridis ssp. viridis Bark" in Journal of Natural Products, 80, no. 5 (2017):1255-1263,
https://doi.org/10.1021/acs.jnatprod.6b00805 . .

TY  - JOUR
AU  - Rajković, Katarina M.
AU  - Jeremić, Sanja
AU  - Milić, Petar S.
AU  - Kostić, Marina
AU  - Arsić-Arsenijević, Valentina
AU  - Gavrilović, Marijana
AU  - Krstić, Boro
PY  - 2017
UR  - https://pp.bme.hu/ch/article/view/9580
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2788
AB  - Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady’s Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solvent-to-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40oC and S of 30 kg kg-1. Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry.
T2  - Periodica Polytechnica Chemical Engineering
T1  - Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.
IS  - 3
VL  - 61
DO  - 10.3311/PPch.9580
SP  - 200
EP  - 205
ER  - 

@article{
author = "Rajković, Katarina M. and Jeremić, Sanja and Milić, Petar S. and Kostić, Marina and Arsić-Arsenijević, Valentina and Gavrilović, Marijana and Krstić, Boro",
year = "2017",
abstract = "Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady’s Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solvent-to-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40oC and S of 30 kg kg-1. Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry.",
journal = "Periodica Polytechnica Chemical Engineering",
title = "Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.",
number = "3",
volume = "61",
doi = "10.3311/PPch.9580",
pages = "200-205"
}

Rajković, K. M., Jeremić, S., Milić, P. S., Kostić, M., Arsić-Arsenijević, V., Gavrilović, M.,& Krstić, B.. (2017). Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.. in Periodica Polytechnica Chemical Engineering, 61(3), 200-205.
https://doi.org/10.3311/PPch.9580

Rajković KM, Jeremić S, Milić PS, Kostić M, Arsić-Arsenijević V, Gavrilović M, Krstić B. Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.. in Periodica Polytechnica Chemical Engineering. 2017;61(3):200-205.
doi:10.3311/PPch.9580 .

Rajković, Katarina M., Jeremić, Sanja, Milić, Petar S., Kostić, Marina, Arsić-Arsenijević, Valentina, Gavrilović, Marijana, Krstić, Boro, "Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L." in Periodica Polytechnica Chemical Engineering, 61, no. 3 (2017):200-205,
https://doi.org/10.3311/PPch.9580 . .