TY  - JOUR
AU  - Stanković, Jovana
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Zdunić, Gordana
AU  - Dajić-Stevanović, Zora
AU  - Gođevac, Dejan
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3613
AB  - This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.
T2  - Journal of the Serbian Chemical Society
T1  - HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
IS  - 12
VL  - 84
DO  - 10.2298/jsc190513106s
SP  - 1355
EP  - 1365
ER  - 

@article{
author = "Stanković, Jovana and Novaković, Miroslav and Tešević, Vele and Ćirić, Ana and Soković, Marina and Zdunić, Gordana and Dajić-Stevanović, Zora and Gođevac, Dejan",
year = "2019",
abstract = "This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.",
journal = "Journal of the Serbian Chemical Society",
title = "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil",
number = "12",
volume = "84",
doi = "10.2298/jsc190513106s",
pages = "1355-1365"
}

Stanković, J., Novaković, M., Tešević, V., Ćirić, A., Soković, M., Zdunić, G., Dajić-Stevanović, Z.,& Gođevac, D.. (2019). HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society, 84(12), 1355-1365.
https://doi.org/10.2298/jsc190513106s

Stanković J, Novaković M, Tešević V, Ćirić A, Soković M, Zdunić G, Dajić-Stevanović Z, Gođevac D. HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society. 2019;84(12):1355-1365.
doi:10.2298/jsc190513106s .

Stanković, Jovana, Novaković, Miroslav, Tešević, Vele, Ćirić, Ana, Soković, Marina, Zdunić, Gordana, Dajić-Stevanović, Zora, Gođevac, Dejan, "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil" in Journal of the Serbian Chemical Society, 84, no. 12 (2019):1355-1365,
https://doi.org/10.2298/jsc190513106s . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Gođevac, Dejan
AU  - Tešević, Vele
AU  - Dajić-Stevanović, Zora
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Novaković, Miroslav
PY  - 2019
UR  - http://pubs.acs.org/doi/10.1021/acs.jnatprod.8b00970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3397
AB  - A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.
PB  - American Chemical Society and American Society of Pharmacognosy
T2  - Journal of Natural Products
T1  - Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.
IS  - 6
VL  - 82
DO  - 10.1021/acs.jnatprod.8b00970
SP  - 1487
EP  - 1495
ER  - 

@article{
author = "Stanković, Jovana and Gođevac, Dejan and Tešević, Vele and Dajić-Stevanović, Zora and Ćirić, Ana and Soković, Marina and Novaković, Miroslav",
year = "2019",
abstract = "A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.",
publisher = "American Chemical Society and American Society of Pharmacognosy",
journal = "Journal of Natural Products",
title = "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.",
number = "6",
volume = "82",
doi = "10.1021/acs.jnatprod.8b00970",
pages = "1487-1495"
}

Stanković, J., Gođevac, D., Tešević, V., Dajić-Stevanović, Z., Ćirić, A., Soković, M.,& Novaković, M.. (2019). Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products
American Chemical Society and American Society of Pharmacognosy., 82(6), 1487-1495.
https://doi.org/10.1021/acs.jnatprod.8b00970

Stanković J, Gođevac D, Tešević V, Dajić-Stevanović Z, Ćirić A, Soković M, Novaković M. Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products. 2019;82(6):1487-1495.
doi:10.1021/acs.jnatprod.8b00970 .

Stanković, Jovana, Gođevac, Dejan, Tešević, Vele, Dajić-Stevanović, Zora, Ćirić, Ana, Soković, Marina, Novaković, Miroslav, "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa." in Journal of Natural Products, 82, no. 6 (2019):1487-1495,
https://doi.org/10.1021/acs.jnatprod.8b00970 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Pećinar, Ilinka
AU  - Brkušanin, Miloš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Dajić Stevanović, Zora
AU  - Schulz, Hartwig
AU  - Mišić, Danijela
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018302413?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3022
AB  - Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklić & Milojević, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental- regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrIS1. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.
T2  - Industrial Crops and Products
T1  - Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants
VL  - 117
DO  - 10.1016/J.INDCROP.2018.03.019
SP  - 347
EP  - 358
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Pećinar, Ilinka and Brkušanin, Miloš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Dajić Stevanović, Zora and Schulz, Hartwig and Mišić, Danijela",
year = "2018",
abstract = "Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklić & Milojević, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental- regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrIS1. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.",
journal = "Industrial Crops and Products",
title = "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants",
volume = "117",
doi = "10.1016/J.INDCROP.2018.03.019",
pages = "347-358"
}

Aničić, N., Matekalo, D., Skorić, M., Pećinar, I., Brkušanin, M., Nestorović Živković, J., Dmitrović, S., Dajić Stevanović, Z., Schulz, H.,& Mišić, D.. (2018). Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products, 117, 347-358.
https://doi.org/10.1016/J.INDCROP.2018.03.019

Aničić N, Matekalo D, Skorić M, Pećinar I, Brkušanin M, Nestorović Živković J, Dmitrović S, Dajić Stevanović Z, Schulz H, Mišić D. Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products. 2018;117:347-358.
doi:10.1016/J.INDCROP.2018.03.019 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Pećinar, Ilinka, Brkušanin, Miloš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Dajić Stevanović, Zora, Schulz, Hartwig, Mišić, Danijela, "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants" in Industrial Crops and Products, 117 (2018):347-358,
https://doi.org/10.1016/J.INDCROP.2018.03.019 . .