TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Vaclav
AU  - Dragićević, Ivana
AU  - Paunović, Danijela
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6322
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.
PB  - elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6322
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Motyka, Vaclav and Dragićević, Ivana and Paunović, Danijela and Marković, Marija and Subotić, Angelina",
year = "2018",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.",
publisher = "elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6322"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I., Paunović, D., Marković, M.,& Subotić, A.. (2018). Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322

Trifunović-Momčilov M, Motyka V, Dragićević I, Paunović D, Marković M, Subotić A. Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

Trifunović-Momčilov, Milana, Motyka, Vaclav, Dragićević, Ivana, Paunović, Danijela, Marković, Marija, Subotić, Angelina, "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):33,
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .