TY  - CONF
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Kukobat, Mila
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Tanasković, Marija
AU  - Stamenković-Radak, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4254
AB  - Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
T1  - Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia
IS  - 1
VL  - 43
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4254
ER  - 

@conference{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Kukobat, Mila and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Tanasković, Marija and Stamenković-Radak, Marina",
year = "2021",
abstract = "Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia",
title = "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia",
number = "1",
volume = "43",
pages = "117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4254"
}

Davidović, S., Jelić, M., Marinković, S., Kukobat, M., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M., Tanasković, M.,& Stamenković-Radak, M.. (2021). Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254

Davidović S, Jelić M, Marinković S, Kukobat M, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Tanasković M, Stamenković-Radak M. Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia. 2021;43(1):117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Kukobat, Mila, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Tanasković, Marija, Stamenković-Radak, Marina, "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia" in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia, 43, no. 1 (2021):117,
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Stamenković-Radak, Marina
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4016
AB  - The Griffon vulture was once a widespread species across the region of Southeast Europe, but it is now endangered and in some parts is completely extinct. In the Balkan Peninsula the largest Griffon vulture inland population inhabits the territory of Serbia. We present, for the first time, the genetic data of this valuable population that could be a source for future reintroduction programs planned in South‑eastern Europe. To characterize the genetic structure of this population we used microsatellite markers from ten loci. Blood samples were collected from 57 chicks directly in the nests during the ongoing monitoring program. We performed a comparative analysis of the obtained data with the existing data from three native populations from French Pyrenees, Croatia, and Israel. We have assessed the genetic differentiation between different native populations and determined the existence of two genetic clusters that differentiate the populations from the Balkan and Iberian Peninsulas. Furthermore, we analysed whether the recent bottleneck events influenced the genetic structure of the populations studied, and we found that all native populations experienced a recent bottleneck event, and that the population of Israel was the least affected. Nevertheless, the parameters of genetic diversity suggest that all analysed populations have retained a similar level of genetic diversity and that the Griffon vulture population from Serbia exhibits the highest value for private alleles. The results of this study suggest that the Griffon vulture populations of the Balkan Peninsula are genetically differentiated from the populations of the Iberian Peninsula, which is an important information for future reintroduction strategies.
PB  - Nature Research
T2  - Scientific Reports
T1  - Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-77342-1
SP  - 20394
ER  - 

@article{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Stamenković-Radak, Marina",
year = "2020",
abstract = "The Griffon vulture was once a widespread species across the region of Southeast Europe, but it is now endangered and in some parts is completely extinct. In the Balkan Peninsula the largest Griffon vulture inland population inhabits the territory of Serbia. We present, for the first time, the genetic data of this valuable population that could be a source for future reintroduction programs planned in South‑eastern Europe. To characterize the genetic structure of this population we used microsatellite markers from ten loci. Blood samples were collected from 57 chicks directly in the nests during the ongoing monitoring program. We performed a comparative analysis of the obtained data with the existing data from three native populations from French Pyrenees, Croatia, and Israel. We have assessed the genetic differentiation between different native populations and determined the existence of two genetic clusters that differentiate the populations from the Balkan and Iberian Peninsulas. Furthermore, we analysed whether the recent bottleneck events influenced the genetic structure of the populations studied, and we found that all native populations experienced a recent bottleneck event, and that the population of Israel was the least affected. Nevertheless, the parameters of genetic diversity suggest that all analysed populations have retained a similar level of genetic diversity and that the Griffon vulture population from Serbia exhibits the highest value for private alleles. The results of this study suggest that the Griffon vulture populations of the Balkan Peninsula are genetically differentiated from the populations of the Iberian Peninsula, which is an important information for future reintroduction strategies.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-77342-1",
pages = "20394"
}

Davidović, S., Jelić, M., Marinković, S., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M.,& Stamenković-Radak, M.. (2020). Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. in Scientific Reports
Nature Research., 10(1), 20394.
https://doi.org/10.1038/s41598-020-77342-1

Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. in Scientific Reports. 2020;10(1):20394.
doi:10.1038/s41598-020-77342-1 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Stamenković-Radak, Marina, "Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans" in Scientific Reports, 10, no. 1 (2020):20394,
https://doi.org/10.1038/s41598-020-77342-1 . .

TY  - DATA
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Stamenković-Radak, Marina
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4183
AB  - Table S1. Parameters of genetic diversity calculated per locus per populations including Hardy-Weinberg equilibrium test and Garza Williamson index. Legend: N - number of gene copies, A - number of alleles, Ho – observed heterozygosity, He – expected heterozygosity, H-W - test for Hardy-Weinberg equilibrium, Ae - effective number of alleles, Ar - allelic range, G-W Garza-Williamson index; Table S2. Number of alleles per locus per populations; Table S3. Tables of significant linkage disequilibrium (significance level p=0.0500, corrected using sequential Bonferroni test). Detected linkage disequlibrium is marked with pink colour; Table S1. Effective population sizes estimated for analysed populations. Legend: N - number of individuals in sample, Ne - effective population size; Table S5. Outcomes of AMOVA analysis based on the variability of 10 autosomal loci for different groups of populations.
PB  - Nature Research
T2  - Scientific Reports
T1  - Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.
IS  - 1
VL  - 10
SP  - 20394
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4183
ER  - 

@misc{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Stamenković-Radak, Marina",
year = "2020",
abstract = "Table S1. Parameters of genetic diversity calculated per locus per populations including Hardy-Weinberg equilibrium test and Garza Williamson index. Legend: N - number of gene copies, A - number of alleles, Ho – observed heterozygosity, He – expected heterozygosity, H-W - test for Hardy-Weinberg equilibrium, Ae - effective number of alleles, Ar - allelic range, G-W Garza-Williamson index; Table S2. Number of alleles per locus per populations; Table S3. Tables of significant linkage disequilibrium (significance level p=0.0500, corrected using sequential Bonferroni test). Detected linkage disequlibrium is marked with pink colour; Table S1. Effective population sizes estimated for analysed populations. Legend: N - number of individuals in sample, Ne - effective population size; Table S5. Outcomes of AMOVA analysis based on the variability of 10 autosomal loci for different groups of populations.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.",
number = "1",
volume = "10",
pages = "20394",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4183"
}

Davidović, S., Jelić, M., Marinković, S., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M.,& Stamenković-Radak, M.. (2020). Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports
Nature Research., 10(1), 20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4183

Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports. 2020;10(1):20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4183 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Stamenković-Radak, Marina, "Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394." in Scientific Reports, 10, no. 1 (2020):20394,
https://hdl.handle.net/21.15107/rcub_ibiss_4183 .

TY  - DATA
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Stamenković-Radak, Marina
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4017
AB  - Figure  S1Matrix  of the average number of pairwise differences and Nei's distances detected in native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  microsatellite  loci.  The  average  number  of  pairwise  differences  between  populations  is  presented  above  diagonal,  the  average  number  of  pairwise  differences  within  the  population  is  presented  diagonal  and  Nei's  distances  are  presented  below  diagonal.  The  average  number  of  pairwise  differences  are  presented in Table 1.; Figure S2 Matrix of pairwise FSTdistances between the G. fulvus population of Serbia and other native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  10  microsatellite  loci.Statist ically significant FST values are marked with an asterisk (*). Population pairwise FSTvalues are presented in Table 3.; Figure S3 Ln values of probability for the assumed number of genetic clusters. Figure S4   (a) Proportions of inferred STRUCTURE clusters (K=2) from the native and introduced populations.(b) Proportions of the inferred STRUCTURE clusters (K=2) from the individuals.
PB  - Nature Research
T2  - Scientific Reports
T1  - Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.
IS  - 1
VL  - 10
SP  - 20394
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4017
ER  - 

@misc{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Stamenković-Radak, Marina",
year = "2020",
abstract = "Figure  S1Matrix  of the average number of pairwise differences and Nei's distances detected in native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  microsatellite  loci.  The  average  number  of  pairwise  differences  between  populations  is  presented  above  diagonal,  the  average  number  of  pairwise  differences  within  the  population  is  presented  diagonal  and  Nei's  distances  are  presented  below  diagonal.  The  average  number  of  pairwise  differences  are  presented in Table 1.; Figure S2 Matrix of pairwise FSTdistances between the G. fulvus population of Serbia and other native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  10  microsatellite  loci.Statist ically significant FST values are marked with an asterisk (*). Population pairwise FSTvalues are presented in Table 3.; Figure S3 Ln values of probability for the assumed number of genetic clusters. Figure S4   (a) Proportions of inferred STRUCTURE clusters (K=2) from the native and introduced populations.(b) Proportions of the inferred STRUCTURE clusters (K=2) from the individuals.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.",
number = "1",
volume = "10",
pages = "20394",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4017"
}

Davidović, S., Jelić, M., Marinković, S., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M.,& Stamenković-Radak, M.. (2020). Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports
Nature Research., 10(1), 20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4017

Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports. 2020;10(1):20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4017 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Stamenković-Radak, Marina, "Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394." in Scientific Reports, 10, no. 1 (2020):20394,
https://hdl.handle.net/21.15107/rcub_ibiss_4017 .