TY  - JOUR
AU  - Stikić, Radmila I.
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Jovanović, Zorica B.
AU  - Gašić, Uroš
AU  - Tešić, Živoslav Lj.
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana K.
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cche.10278
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3639
AB  - Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
IS  - 3
VL  - 97
DO  - 10.1002/cche.10278
SP  - cche.10278
SP  - 626
EP  - 633
ER  - 

@article{
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica B. and Gašić, Uroš and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Sladjana K. and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
number = "3",
volume = "97",
doi = "10.1002/cche.10278",
pages = "cche.10278-626-633"
}

Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z. B., Gašić, U., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry, 97(3), cche.10278-633.
https://doi.org/10.1002/cche.10278

Stikić RI, Milinčić DD, Kostić AŽ, Jovanović ZB, Gašić U, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):cche.10278-633.
doi:10.1002/cche.10278 .

Stikić, Radmila I., Milinčić, Danijel D., Kostić, Aleksandar Ž., Jovanović, Zorica B., Gašić, Uroš, Tešić, Živoslav Lj., Djordjević, Nataša Z., Savić, Sladjana K., Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):cche.10278-633,
https://doi.org/10.1002/cche.10278 . .

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Jarić, Snežana
AU  - Dajić-Stevanović, Zora
AU  - Duletić-Laušević, Sonja
PY  - 2019
UR  - http://link.springer.com/10.1007/s10722-018-00734-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3312
AB  - This work represents an ethnobotanical and ethnomedicinal study focused on recording autochthonous pear varieties of the Polimlje region in southwest Serbia. The purpose of the study was to highlight genetic resources of pear varieties in situ and emphasize aspects of ethnodiversity and traditional knowledge. A special feature of the research area is the great diversity of indigenous varieties of pear, which have been cultivated for hundreds of years. Forty-six respondents from 25 to 78 years of age residing in 23 villages were interviewed, and 26 autochthonous pear varieties were recorded in the field. Among them 11.5% are widely available, 26.9% are rare, and 61.5% are very rare. For ethnomedicinal purposes, pear is used as a remedy against hypertension, diabetes, high cholesterol, and constipation; for reduction of body mass; as a uroseptic and/or an antirheumatic; and for cancer prevention. Pears are consumed as fresh fruit and in numerous food products. Depopulation of the investigated rural area is directly responsible for depletion of the gene pool of pear varieties and for loss of traditional knowledge. Preservation can be achieved by in situ methods such as raising awareness of the local inhabitants and ensuring support of the State.
T2  - Genetic Resources and Crop Evolution
T1  - Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje)
IS  - 3
VL  - 66
DO  - 10.1007/s10722-018-00734-w
SP  - 589
EP  - 609
ER  - 

@article{
author = "Savić, Aleksandra and Jarić, Snežana and Dajić-Stevanović, Zora and Duletić-Laušević, Sonja",
year = "2019",
abstract = "This work represents an ethnobotanical and ethnomedicinal study focused on recording autochthonous pear varieties of the Polimlje region in southwest Serbia. The purpose of the study was to highlight genetic resources of pear varieties in situ and emphasize aspects of ethnodiversity and traditional knowledge. A special feature of the research area is the great diversity of indigenous varieties of pear, which have been cultivated for hundreds of years. Forty-six respondents from 25 to 78 years of age residing in 23 villages were interviewed, and 26 autochthonous pear varieties were recorded in the field. Among them 11.5% are widely available, 26.9% are rare, and 61.5% are very rare. For ethnomedicinal purposes, pear is used as a remedy against hypertension, diabetes, high cholesterol, and constipation; for reduction of body mass; as a uroseptic and/or an antirheumatic; and for cancer prevention. Pears are consumed as fresh fruit and in numerous food products. Depopulation of the investigated rural area is directly responsible for depletion of the gene pool of pear varieties and for loss of traditional knowledge. Preservation can be achieved by in situ methods such as raising awareness of the local inhabitants and ensuring support of the State.",
journal = "Genetic Resources and Crop Evolution",
title = "Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje)",
number = "3",
volume = "66",
doi = "10.1007/s10722-018-00734-w",
pages = "589-609"
}

Savić, A., Jarić, S., Dajić-Stevanović, Z.,& Duletić-Laušević, S.. (2019). Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje). in Genetic Resources and Crop Evolution, 66(3), 589-609.
https://doi.org/10.1007/s10722-018-00734-w

Savić A, Jarić S, Dajić-Stevanović Z, Duletić-Laušević S. Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje). in Genetic Resources and Crop Evolution. 2019;66(3):589-609.
doi:10.1007/s10722-018-00734-w .

Savić, Aleksandra, Jarić, Snežana, Dajić-Stevanović, Zora, Duletić-Laušević, Sonja, "Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje)" in Genetic Resources and Crop Evolution, 66, no. 3 (2019):589-609,
https://doi.org/10.1007/s10722-018-00734-w . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Pećinar, Ilinka
AU  - Brkušanin, Miloš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Dajić Stevanović, Zora
AU  - Schulz, Hartwig
AU  - Mišić, Danijela
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018302413?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3022
AB  - Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklić & Milojević, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental- regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrIS1. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.
T2  - Industrial Crops and Products
T1  - Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants
VL  - 117
DO  - 10.1016/J.INDCROP.2018.03.019
SP  - 347
EP  - 358
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Pećinar, Ilinka and Brkušanin, Miloš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Dajić Stevanović, Zora and Schulz, Hartwig and Mišić, Danijela",
year = "2018",
abstract = "Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklić & Milojević, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental- regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrIS1. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.",
journal = "Industrial Crops and Products",
title = "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants",
volume = "117",
doi = "10.1016/J.INDCROP.2018.03.019",
pages = "347-358"
}

Aničić, N., Matekalo, D., Skorić, M., Pećinar, I., Brkušanin, M., Nestorović Živković, J., Dmitrović, S., Dajić Stevanović, Z., Schulz, H.,& Mišić, D.. (2018). Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products, 117, 347-358.
https://doi.org/10.1016/J.INDCROP.2018.03.019

Aničić N, Matekalo D, Skorić M, Pećinar I, Brkušanin M, Nestorović Živković J, Dmitrović S, Dajić Stevanović Z, Schulz H, Mišić D. Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products. 2018;117:347-358.
doi:10.1016/J.INDCROP.2018.03.019 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Pećinar, Ilinka, Brkušanin, Miloš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Dajić Stevanović, Zora, Schulz, Hartwig, Mišić, Danijela, "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants" in Industrial Crops and Products, 117 (2018):347-358,
https://doi.org/10.1016/J.INDCROP.2018.03.019 . .