@inbook{
editor = "Abdurakhmonov, Ibrokhim Y.",
author = "Trifunović-Momčilov, Milana and Motyka, Václav",
year = "2021",
abstract = "The production and breeding of plants with desired properties are possible by a
fundamental biotechnological technique, genetic engineering. Applying and developing
of genetic engineering procedures also enable preservation and improvement
of plant species endangered in nature, including medicinal plant common centaury
(Centaurium erythraea Rafn.). Numerous developmental processes in plants are
controlled by cytokinins (CKs). The only so far known enzyme involved in CK
catabolism is cytokinin oxidase/dehydrogenase (CKX). Genes coding for two
Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were successfully introduced into
centaury root explants. Subsequently, the contents of endogenous CKs in AtCKXoverexpressing
centaury plants grown in vitro were investigated. Simultaneous
secondary metabolite analyses showed antibacterial and antifungal activity of
transgenic centaury plants and suggested their use as potential producers of anticancer
compounds. Considering that centaury can inhabit saline soils in natural
habitats, following investigations included evaluation of salinity tolerance in vitro.
All obtained and summarized results indicated that transgenic AtCKX centaury
plants could serve as a suitable model for studies of numerous physiological and
developmental processes under endogenous phytohormonal control.",
publisher = "IntechOpen",
journal = "Plant Breeding: Current and Future Views",
booktitle = "Transgenic AtCKX Centaury Plants Grown In Vitro",
doi = "10.5772/intechopen.94836"
}