TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5156
AB  - Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Chemical composition and biological activity of two Lactarius species from Serbia.
SP  - 102
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5156
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Caleja, Cristina and Pereira, Eliana and Barros, Lillian and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina",
year = "2022",
abstract = "Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Chemical composition and biological activity of two Lactarius species from Serbia.",
pages = "102-103",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5156"
}

Kostić, M., Ivanov, M., Ćirić, A., Caleja, C., Pereira, E., Barros, L., Stojković, D., Soković, M.,& Glamočlija, J.. (2022). Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156

Kostić M, Ivanov M, Ćirić A, Caleja C, Pereira E, Barros L, Stojković D, Soković M, Glamočlija J. Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Caleja, Cristina, Pereira, Eliana, Barros, Lillian, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, "Chemical composition and biological activity of two Lactarius species from Serbia." in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):102-103,
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

TY  - CONF
AU  - Stojković, Dejan
AU  - Kostić, Marina
AU  - Fernandes, Angela
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5157
AB  - Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5157
ER  - 

@conference{
author = "Stojković, Dejan and Kostić, Marina and Fernandes, Angela and Barros, Lillian and Glamočlija, Jasmina",
year = "2022",
abstract = "Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5157"
}

Stojković, D., Kostić, M., Fernandes, A., Barros, L.,& Glamočlija, J.. (2022). Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157

Stojković D, Kostić M, Fernandes A, Barros L, Glamočlija J. Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

Stojković, Dejan, Kostić, Marina, Fernandes, Angela, Barros, Lillian, Glamočlija, Jasmina, "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides" in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):168,
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Arampatzis, Dimitrios A.
AU  - Tsiropoulos, Nikolaos G.
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - internal-pdf://Petropoulos et al. - 2020 - Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.) A new insight to plant-based sou.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3602
AB  - In the present study, nutritional value, chemical composition and bioactive properties of purslane seeds, seed oils and seedcakes were examined. Data were analyzed by a one-way ANOVA, while means were compared with Tukey's HSD test. For seed oil extraction mechanical and ultrasound assisted methods were tested. Cold extraction methods (CE1 and CE2) resulted in higher oil yield (increased by 33.7% and 38.1%, respectively) comparing to hot extraction (HE) method. Seeds contained the highest amount of fats and energy (15.03 ± 0.06 g/100 g dry basis (db) and 459 ± 1 kcal/100 g db, respectively), while seedcakes from CE2 had the highest content in proteins and ash (31.20 ± 0.03 and 4.27 ± 0.06 g/100 g db, respectively). Seeds and seedcakes contained a balanced content of linoleic and α-linolenic acids (33.80–34.74% and 32.83–34.64%, respectively). HE and CE1 oils had slightly higher amounts of α-linolenic (39.67% and 39.57%, respectively) than linoleic acid (35.44% and 35.13%, respectively), whereas CE2 oils contained twice as much linoleic as α-linolenic acid (49.77% and 24.18%, respectively). In conclusion, the tested materials are good sources of omega-6 and omega-3 fatty acids and proteins, while extraction method affected oil yield and fatty acids composition of seed oils.
T2  - LWT
T1  - Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids
VL  - 123
DO  - 10.1016/j.lwt.2020.109099
SP  - 109099
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Arampatzis, Dimitrios A. and Tsiropoulos, Nikolaos G. and Petrović, Jovana and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "In the present study, nutritional value, chemical composition and bioactive properties of purslane seeds, seed oils and seedcakes were examined. Data were analyzed by a one-way ANOVA, while means were compared with Tukey's HSD test. For seed oil extraction mechanical and ultrasound assisted methods were tested. Cold extraction methods (CE1 and CE2) resulted in higher oil yield (increased by 33.7% and 38.1%, respectively) comparing to hot extraction (HE) method. Seeds contained the highest amount of fats and energy (15.03 ± 0.06 g/100 g dry basis (db) and 459 ± 1 kcal/100 g db, respectively), while seedcakes from CE2 had the highest content in proteins and ash (31.20 ± 0.03 and 4.27 ± 0.06 g/100 g db, respectively). Seeds and seedcakes contained a balanced content of linoleic and α-linolenic acids (33.80–34.74% and 32.83–34.64%, respectively). HE and CE1 oils had slightly higher amounts of α-linolenic (39.67% and 39.57%, respectively) than linoleic acid (35.44% and 35.13%, respectively), whereas CE2 oils contained twice as much linoleic as α-linolenic acid (49.77% and 24.18%, respectively). In conclusion, the tested materials are good sources of omega-6 and omega-3 fatty acids and proteins, while extraction method affected oil yield and fatty acids composition of seed oils.",
journal = "LWT",
title = "Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids",
volume = "123",
doi = "10.1016/j.lwt.2020.109099",
pages = "109099"
}

Petropoulos, S. A., Fernandes, Â., Arampatzis, D. A., Tsiropoulos, N. G., Petrović, J., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2020). Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids. in LWT, 123, 109099.
https://doi.org/10.1016/j.lwt.2020.109099

Petropoulos SA, Fernandes Â, Arampatzis DA, Tsiropoulos NG, Petrović J, Soković M, Barros L, Ferreira IC. Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids. in LWT. 2020;123:109099.
doi:10.1016/j.lwt.2020.109099 .

Petropoulos, Spyridon A., Fernandes, Ângela, Arampatzis, Dimitrios A., Tsiropoulos, Nikolaos G., Petrović, Jovana, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids" in LWT, 123 (2020):109099,
https://doi.org/10.1016/j.lwt.2020.109099 . .

TY  - JOUR
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Petropoulos, Spyridon A.
AU  - Plexida, Sofia
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikos
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - C F R Ferreira, Isabel
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4494
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3577
AB  - In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244494
SP  - 4494
ER  - 

@article{
author = "Pereira, Carla and Dias, Maria Inês and Petropoulos, Spyridon A. and Plexida, Sofia and Chrysargyris, Antonios and Tzortzakis, Nikos and Calhelha, Ricardo C. and Ivanov, Marija and Stojković, Dejan and Soković, Marina and Barros, Lillian and C F R Ferreira, Isabel",
year = "2019",
abstract = "In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).",
number = "24",
volume = "24",
doi = "10.3390/molecules24244494",
pages = "4494"
}

Pereira, C., Dias, M. I., Petropoulos, S. A., Plexida, S., Chrysargyris, A., Tzortzakis, N., Calhelha, R. C., Ivanov, M., Stojković, D., Soković, M., Barros, L.,& C F R Ferreira, I.. (2019). The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4494.
https://doi.org/10.3390/molecules24244494

Pereira C, Dias MI, Petropoulos SA, Plexida S, Chrysargyris A, Tzortzakis N, Calhelha RC, Ivanov M, Stojković D, Soković M, Barros L, C F R Ferreira I. The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland). 2019;24(24):4494.
doi:10.3390/molecules24244494 .

Pereira, Carla, Dias, Maria Inês, Petropoulos, Spyridon A., Plexida, Sofia, Chrysargyris, Antonios, Tzortzakis, Nikos, Calhelha, Ricardo C., Ivanov, Marija, Stojković, Dejan, Soković, Marina, Barros, Lillian, C F R Ferreira, Isabel, "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4494,
https://doi.org/10.3390/molecules24244494 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Kruljević, Ivana
AU  - Stojković, Dejan
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2019
UR  - http://xlink.rsc.org/?DOI=C9FO01900F
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3576
AB  - This study was oriented towards the investigation of the biological properties of three wild growing and edible Macrolepiota species (M. mastoidea, M. rhacodes and Macrolepiota procera) from Serbia. The results revealed that the mushrooms have a low caloric value; free sugars such as mannitol and trehalose were identified; oxalic and malic acids were predominant organic acids, while p-hydroxybenzoic and p-coumaric acids were identified as the main phenolic compounds. Also, they were a rich source of polyunsaturated fatty acids, which dominated over monounsaturated and saturated fatty acids. Three isoforms of tocopherols were identified and quantified: α-, β-, and δ-tocopherol. Regarding biological properties, all three species exhibited antioxidant potential, antimicrobial potential and cytotoxic activity within the different tumour cell lines tested. This study indicates that these species are indeed functional foods, due to the fact that they are edible, consumable and hold different pharmacological activities.
PB  - The Royal Society of Chemistry
T2  - Food & Function
T1  - Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.
IS  - 12
VL  - 10
DO  - 10.1039/c9fo01900f
SP  - 7678
EP  - 7686
ER  - 

@article{
author = "Ćirić, Ana and Kruljević, Ivana and Stojković, Dejan and Fernandes, Ângela and Barros, Lillian and Calhelha, Ricardo C. and Ferreira, Isabel C. F. R. and Soković, Marina and Glamočlija, Jasmina",
year = "2019",
abstract = "This study was oriented towards the investigation of the biological properties of three wild growing and edible Macrolepiota species (M. mastoidea, M. rhacodes and Macrolepiota procera) from Serbia. The results revealed that the mushrooms have a low caloric value; free sugars such as mannitol and trehalose were identified; oxalic and malic acids were predominant organic acids, while p-hydroxybenzoic and p-coumaric acids were identified as the main phenolic compounds. Also, they were a rich source of polyunsaturated fatty acids, which dominated over monounsaturated and saturated fatty acids. Three isoforms of tocopherols were identified and quantified: α-, β-, and δ-tocopherol. Regarding biological properties, all three species exhibited antioxidant potential, antimicrobial potential and cytotoxic activity within the different tumour cell lines tested. This study indicates that these species are indeed functional foods, due to the fact that they are edible, consumable and hold different pharmacological activities.",
publisher = "The Royal Society of Chemistry",
journal = "Food & Function",
title = "Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.",
number = "12",
volume = "10",
doi = "10.1039/c9fo01900f",
pages = "7678-7686"
}

Ćirić, A., Kruljević, I., Stojković, D., Fernandes, Â., Barros, L., Calhelha, R. C., Ferreira, I. C. F. R., Soković, M.,& Glamočlija, J.. (2019). Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.. in Food & Function
The Royal Society of Chemistry., 10(12), 7678-7686.
https://doi.org/10.1039/c9fo01900f

Ćirić A, Kruljević I, Stojković D, Fernandes Â, Barros L, Calhelha RC, Ferreira ICFR, Soković M, Glamočlija J. Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.. in Food & Function. 2019;10(12):7678-7686.
doi:10.1039/c9fo01900f .

Ćirić, Ana, Kruljević, Ivana, Stojković, Dejan, Fernandes, Ângela, Barros, Lillian, Calhelha, Ricardo C., Ferreira, Isabel C. F. R., Soković, Marina, Glamočlija, Jasmina, "Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities." in Food & Function, 10, no. 12 (2019):7678-7686,
https://doi.org/10.1039/c9fo01900f . .

TY  - JOUR
AU  - Petropoulos, Spyridon
AU  - Fernandes, Ângela
AU  - Pereira, Carla
AU  - Tzortzakis, Nikos
AU  - Vaz, Josiana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0308814619305436?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3314
AB  - In the present study, the nutritional value, bioactive properties, and chemical composition of various cardoon (Cynara cardunculus L.) genotypes cultivated in central Greece were investigated. The results demonstrated that Cynara seeds are a good source of fat and protein, while they also contain considerable amounts of K, Mg, and Fe and low amount of Na. Sucrose, oxalic acid, and α-tocopherol were the only free sugar, organic acid, and tocopherol isoform respectively, found among the studied genotypes. The most abundant fatty acids were linoleic, oleic and palmitic acid, while PUFA was the most abundant fatty acid class. All the tested seeds contained only two phenolic compounds, namely 5-O-caffeoylquinic acid and 3,5-O-caffeoylquinic acid, while significant antioxidant activities and cytotoxicity against tumor cell lines and antimicrobial effects were also observed. In conclusion, cardoon seed extracts could be exploited in the food and pharmaceutical industries as alternative sources of natural compounds with bioactive properties.
T2  - Food Chemistry
T1  - Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds
VL  - 289
DO  - 10.1016/J.FOODCHEM.2019.03.066
SP  - 404
EP  - 412
ER  - 

@article{
author = "Petropoulos, Spyridon and Fernandes, Ângela and Pereira, Carla and Tzortzakis, Nikos and Vaz, Josiana and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2019",
abstract = "In the present study, the nutritional value, bioactive properties, and chemical composition of various cardoon (Cynara cardunculus L.) genotypes cultivated in central Greece were investigated. The results demonstrated that Cynara seeds are a good source of fat and protein, while they also contain considerable amounts of K, Mg, and Fe and low amount of Na. Sucrose, oxalic acid, and α-tocopherol were the only free sugar, organic acid, and tocopherol isoform respectively, found among the studied genotypes. The most abundant fatty acids were linoleic, oleic and palmitic acid, while PUFA was the most abundant fatty acid class. All the tested seeds contained only two phenolic compounds, namely 5-O-caffeoylquinic acid and 3,5-O-caffeoylquinic acid, while significant antioxidant activities and cytotoxicity against tumor cell lines and antimicrobial effects were also observed. In conclusion, cardoon seed extracts could be exploited in the food and pharmaceutical industries as alternative sources of natural compounds with bioactive properties.",
journal = "Food Chemistry",
title = "Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds",
volume = "289",
doi = "10.1016/J.FOODCHEM.2019.03.066",
pages = "404-412"
}

Petropoulos, S., Fernandes, Â., Pereira, C., Tzortzakis, N., Vaz, J., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2019). Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds. in Food Chemistry, 289, 404-412.
https://doi.org/10.1016/J.FOODCHEM.2019.03.066

Petropoulos S, Fernandes Â, Pereira C, Tzortzakis N, Vaz J, Soković M, Barros L, Ferreira IC. Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds. in Food Chemistry. 2019;289:404-412.
doi:10.1016/J.FOODCHEM.2019.03.066 .

Petropoulos, Spyridon, Fernandes, Ângela, Pereira, Carla, Tzortzakis, Nikos, Vaz, Josiana, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds" in Food Chemistry, 289 (2019):404-412,
https://doi.org/10.1016/J.FOODCHEM.2019.03.066 . .

TY  - JOUR
AU  - Karkanis, Anestis C.
AU  - Fernandes, Ângela
AU  - Vaz, Josiana
AU  - Petropoulos, Spyridon
AU  - Georgiou, Eleftheria
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Oludemi, Taofiq
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2019
UR  - http://xlink.rsc.org/?DOI=C8FO02601G
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3311
AB  - Sanguisorba minor Scop. is a perennial plant native to the Mediterranean region which exhibits several medicinal activities. In the present study, plant growth, chemical composition, and antitumor and antimicrobial properties of S. minor plants were evaluated under different growing conditions. In particular, plants were grown on different substrates, namely (A) peat, (B) peat : perlite (1 : 1) and (C) peat : perlite (2 : 1). The dry weight of the aerial parts and roots was higher for the peat treatment (A), whereas plant growth was severely affected in the peat : perlite (1 : 1) treatment (B). The major detected sugars were fructose and glucose, while the peat : perlite (2 : 1) treatment resulted in a significant increase of α-tocopherol, glucose and total sugars content, especially in the aerial parts. Oxalic acid and citric acid were the major organic acids detected, with a varied effect of growth substrate on organic acid profile. Growth substrate and plant part also had a significant effect on fatty acid composition, especially on linoleic acid and α-linolenic acid content which was higher in the peat : perlite (1 : 1) treatment in both aerial parts and roots. An impact on the composition of phenolic compounds was also observed, where the plants grown on peat : perlite (2 : 1) and peat : perlite (1 : 1) substrates had the highest content of individual and total phenolic compounds in their aerial parts and roots, respectively. Moreover, roots contained significantly higher amounts of phenolic compounds than the aerial parts. Moreover, plant extracts exhibited antiproliferative activity against four tumor cell lines (HeLa, HepG2, MCF-7 and NCI-H460) and a primary culture of porcine liver cells (PLP2), as well as significant antimicrobial properties. In conclusion, S. minor presented significant bioactive properties, while growth substrates affected the nutritional value, chemical composition, antitumor and antimicrobial properties of the species which could be probably attributed to the higher phenolic compound content and different compositions of phenolic compounds. Therefore, although the species is commonly found in the Mediterranean region, it is underexploited yet and its plant tissues could be a potential source of natural bioactive compounds with further use in pharmaceutical and medicinal applications.
T2  - Food & Function
T1  - Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.
IS  - 3
VL  - 10
DO  - 10.1039/c8fo02601g
SP  - 1340
EP  - 1351
ER  - 

@article{
author = "Karkanis, Anestis C. and Fernandes, Ângela and Vaz, Josiana and Petropoulos, Spyridon and Georgiou, Eleftheria and Ćirić, Ana and Soković, Marina and Oludemi, Taofiq and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2019",
abstract = "Sanguisorba minor Scop. is a perennial plant native to the Mediterranean region which exhibits several medicinal activities. In the present study, plant growth, chemical composition, and antitumor and antimicrobial properties of S. minor plants were evaluated under different growing conditions. In particular, plants were grown on different substrates, namely (A) peat, (B) peat : perlite (1 : 1) and (C) peat : perlite (2 : 1). The dry weight of the aerial parts and roots was higher for the peat treatment (A), whereas plant growth was severely affected in the peat : perlite (1 : 1) treatment (B). The major detected sugars were fructose and glucose, while the peat : perlite (2 : 1) treatment resulted in a significant increase of α-tocopherol, glucose and total sugars content, especially in the aerial parts. Oxalic acid and citric acid were the major organic acids detected, with a varied effect of growth substrate on organic acid profile. Growth substrate and plant part also had a significant effect on fatty acid composition, especially on linoleic acid and α-linolenic acid content which was higher in the peat : perlite (1 : 1) treatment in both aerial parts and roots. An impact on the composition of phenolic compounds was also observed, where the plants grown on peat : perlite (2 : 1) and peat : perlite (1 : 1) substrates had the highest content of individual and total phenolic compounds in their aerial parts and roots, respectively. Moreover, roots contained significantly higher amounts of phenolic compounds than the aerial parts. Moreover, plant extracts exhibited antiproliferative activity against four tumor cell lines (HeLa, HepG2, MCF-7 and NCI-H460) and a primary culture of porcine liver cells (PLP2), as well as significant antimicrobial properties. In conclusion, S. minor presented significant bioactive properties, while growth substrates affected the nutritional value, chemical composition, antitumor and antimicrobial properties of the species which could be probably attributed to the higher phenolic compound content and different compositions of phenolic compounds. Therefore, although the species is commonly found in the Mediterranean region, it is underexploited yet and its plant tissues could be a potential source of natural bioactive compounds with further use in pharmaceutical and medicinal applications.",
journal = "Food & Function",
title = "Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.",
number = "3",
volume = "10",
doi = "10.1039/c8fo02601g",
pages = "1340-1351"
}

Karkanis, A. C., Fernandes, Â., Vaz, J., Petropoulos, S., Georgiou, E., Ćirić, A., Soković, M., Oludemi, T., Barros, L.,& Ferreira, I. C. F. R.. (2019). Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.. in Food & Function, 10(3), 1340-1351.
https://doi.org/10.1039/c8fo02601g

Karkanis AC, Fernandes Â, Vaz J, Petropoulos S, Georgiou E, Ćirić A, Soković M, Oludemi T, Barros L, Ferreira ICFR. Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.. in Food & Function. 2019;10(3):1340-1351.
doi:10.1039/c8fo02601g .

Karkanis, Anestis C., Fernandes, Ângela, Vaz, Josiana, Petropoulos, Spyridon, Georgiou, Eleftheria, Ćirić, Ana, Soković, Marina, Oludemi, Taofiq, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions." in Food & Function, 10, no. 3 (2019):1340-1351,
https://doi.org/10.1039/c8fo02601g . .