TY  - JOUR
AU  - Ivanov, Marija
AU  - Kannan, Abhilash
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C.F.R.
AU  - Sanglard, Dominique
AU  - Soković, Marina
PY  - 2021
UR  - https://doi.org/10.3390/ijms22020483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4230
AB  - Candida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity
IS  - 2
VL  - 22
DO  - 10.3390/ijms22020483
SP  - 1
EP  - 14
ER  - 

@article{
author = "Ivanov, Marija and Kannan, Abhilash and Stojković, Dejan and Glamočlija, Jasmina and Calhelha, Ricardo C. and Ferreira, Isabel C.F.R. and Sanglard, Dominique and Soković, Marina",
year = "2021",
abstract = "Candida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity",
number = "2",
volume = "22",
doi = "10.3390/ijms22020483",
pages = "1-14"
}

Ivanov, M., Kannan, A., Stojković, D., Glamočlija, J., Calhelha, R. C., Ferreira, I. C.F.R., Sanglard, D.,& Soković, M.. (2021). Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. in International Journal of Molecular Sciences
MDPI AG., 22(2), 1-14.
https://doi.org/10.3390/ijms22020483

Ivanov M, Kannan A, Stojković D, Glamočlija J, Calhelha RC, Ferreira IC, Sanglard D, Soković M. Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. in International Journal of Molecular Sciences. 2021;22(2):1-14.
doi:10.3390/ijms22020483 .

Ivanov, Marija, Kannan, Abhilash, Stojković, Dejan, Glamočlija, Jasmina, Calhelha, Ricardo C., Ferreira, Isabel C.F.R., Sanglard, Dominique, Soković, Marina, "Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity" in International Journal of Molecular Sciences, 22, no. 2 (2021):1-14,
https://doi.org/10.3390/ijms22020483 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Dias, Maria Inês
AU  - Drakulić, Danijela
AU  - Barros, Lillian
AU  - Stevanović, Milena
AU  - C F R Ferreira, Isabel
AU  - Soković, Marina
PY  - 2020
UR  - https://www.mdpi.com/1424-8247/13/4/78
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32344670
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7243113
UR  - https://radar.ibiss.bg.ac.rs/123456789/3854
AB  - Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
PB  - MDPI AG
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.
IS  - 4
VL  - 13
DO  - 10.3390/ph13040078
SP  - 78
ER  - 

@article{
author = "Stojković, Dejan and Dias, Maria Inês and Drakulić, Danijela and Barros, Lillian and Stevanović, Milena and C F R Ferreira, Isabel and Soković, Marina",
year = "2020",
abstract = "Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.",
publisher = "MDPI AG",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.",
number = "4",
volume = "13",
doi = "10.3390/ph13040078",
pages = "78"
}

Stojković, D., Dias, M. I., Drakulić, D., Barros, L., Stevanović, M., C F R Ferreira, I.,& Soković, M.. (2020). Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.. in Pharmaceuticals (Basel, Switzerland)
MDPI AG., 13(4), 78.
https://doi.org/10.3390/ph13040078

Stojković D, Dias MI, Drakulić D, Barros L, Stevanović M, C F R Ferreira I, Soković M. Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.. in Pharmaceuticals (Basel, Switzerland). 2020;13(4):78.
doi:10.3390/ph13040078 .

Stojković, Dejan, Dias, Maria Inês, Drakulić, Danijela, Barros, Lillian, Stevanović, Milena, C F R Ferreira, Isabel, Soković, Marina, "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells." in Pharmaceuticals (Basel, Switzerland), 13, no. 4 (2020):78,
https://doi.org/10.3390/ph13040078 . .