TY  - JOUR
AU  - Janjić, Marija
AU  - Prévide, Rafael M.
AU  - Fletcher, Patrick A.
AU  - Sherman, Arthur
AU  - Smiljanić, Kosara
AU  - Abebe, Daniel
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6934515
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3595
AB  - Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.
T2  - Scientific Reports
T1  - Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.
IS  - 1
VL  - 9
DO  - 10.1038/s41598-019-56480-1
SP  - 20098
ER  - 

@article{
author = "Janjić, Marija and Prévide, Rafael M. and Fletcher, Patrick A. and Sherman, Arthur and Smiljanić, Kosara and Abebe, Daniel and Bjelobaba, Ivana and Stojilković, Stanko S.",
year = "2019",
abstract = "Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.",
journal = "Scientific Reports",
title = "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.",
number = "1",
volume = "9",
doi = "10.1038/s41598-019-56480-1",
pages = "20098"
}

Janjić, M., Prévide, R. M., Fletcher, P. A., Sherman, A., Smiljanić, K., Abebe, D., Bjelobaba, I.,& Stojilković, S. S.. (2019). Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports, 9(1), 20098.
https://doi.org/10.1038/s41598-019-56480-1

Janjić M, Prévide RM, Fletcher PA, Sherman A, Smiljanić K, Abebe D, Bjelobaba I, Stojilković SS. Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports. 2019;9(1):20098.
doi:10.1038/s41598-019-56480-1 .

Janjić, Marija, Prévide, Rafael M., Fletcher, Patrick A., Sherman, Arthur, Smiljanić, Kosara, Abebe, Daniel, Bjelobaba, Ivana, Stojilković, Stanko S., "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo." in Scientific Reports, 9, no. 1 (2019):20098,
https://doi.org/10.1038/s41598-019-56480-1 . .