TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Romić, Snježana
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5023
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.
PB  - Amsterdam: Elsevier Ltd.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
DO  - 10.1016/j.rvsc.2022.04.012
SP  - 189
EP  - 194
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana and Romić, Snježana and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.",
publisher = "Amsterdam: Elsevier Ltd.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
doi = "10.1016/j.rvsc.2022.04.012",
pages = "189-194"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A., Romić, S., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science
Amsterdam: Elsevier Ltd.., 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević A, Romić S, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana, Romić, Snježana, Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - JOUR
AU  - Đorđević, Iris
AU  - Cvetković, Mirjana
AU  - Jadranin, Milka
AU  - Bojović, Srđan
AU  - Anđelković, Boban
AU  - Sofrenić, Ivana
AU  - Simić, Katarina
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51392100083D
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4763
AB  - Phytochemistry deals with the study of secondary metabolites produced by plants that synthesize these compounds for many reasons, including their own protection against attack of herbivores and plant diseases. Secondary metabolites are believed to represent plant adaptation to various environmental factors and that they enabled the survival of the species. Secondary metabolites of plants can have curative or toxic effects in humans and animals. Herbal medicine has a long tradition in folk medicine and until the early 20th century, when synthetic organic chemistry began to develop, plants were the main source of medicines. The two basic goals of our phytochemical research are: isolation and identification of new (biologically active) compounds ? potential drugs, and chemotaxonomy (chemosystematics). In the following text through one selected example, the genus Amphoricarpos Vis., our phytochemical research is shown on both aspects.
PB  - Belgrade: Serbian Chemical Socitey
T2  - Journal of the Serbian Chemical Society
T1  - Phytochemical study of the genus Amphoricarpos
IS  - 12
VL  - 86
DO  - 10.2298/JSC210726083D
SP  - 1177
EP  - 1193
ER  - 

@article{
author = "Đorđević, Iris and Cvetković, Mirjana and Jadranin, Milka and Bojović, Srđan and Anđelković, Boban and Sofrenić, Ivana and Simić, Katarina",
year = "2021",
abstract = "Phytochemistry deals with the study of secondary metabolites produced by plants that synthesize these compounds for many reasons, including their own protection against attack of herbivores and plant diseases. Secondary metabolites are believed to represent plant adaptation to various environmental factors and that they enabled the survival of the species. Secondary metabolites of plants can have curative or toxic effects in humans and animals. Herbal medicine has a long tradition in folk medicine and until the early 20th century, when synthetic organic chemistry began to develop, plants were the main source of medicines. The two basic goals of our phytochemical research are: isolation and identification of new (biologically active) compounds ? potential drugs, and chemotaxonomy (chemosystematics). In the following text through one selected example, the genus Amphoricarpos Vis., our phytochemical research is shown on both aspects.",
publisher = "Belgrade: Serbian Chemical Socitey",
journal = "Journal of the Serbian Chemical Society",
title = "Phytochemical study of the genus Amphoricarpos",
number = "12",
volume = "86",
doi = "10.2298/JSC210726083D",
pages = "1177-1193"
}

Đorđević, I., Cvetković, M., Jadranin, M., Bojović, S., Anđelković, B., Sofrenić, I.,& Simić, K.. (2021). Phytochemical study of the genus Amphoricarpos. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Socitey., 86(12), 1177-1193.
https://doi.org/10.2298/JSC210726083D

Đorđević I, Cvetković M, Jadranin M, Bojović S, Anđelković B, Sofrenić I, Simić K. Phytochemical study of the genus Amphoricarpos. in Journal of the Serbian Chemical Society. 2021;86(12):1177-1193.
doi:10.2298/JSC210726083D .

Đorđević, Iris, Cvetković, Mirjana, Jadranin, Milka, Bojović, Srđan, Anđelković, Boban, Sofrenić, Ivana, Simić, Katarina, "Phytochemical study of the genus Amphoricarpos" in Journal of the Serbian Chemical Society, 86, no. 12 (2021):1177-1193,
https://doi.org/10.2298/JSC210726083D . .

TY  - JOUR
AU  - Ćupić Miladinović, Dejana
AU  - Prevendar Crnić, Andreja
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Ivanović, Saša
AU  - Santibanez, Juan Francisco
AU  - Ćupić, Vitomir
AU  - Borozan, Nevena
AU  - Antonijević Miljaković, Evica
AU  - Borozan, Sunčica
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0009279720315908
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33166511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4007
AB  - Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.
PB  - Elsevier BV
T2  - Chemico-Biological Interactions
T1  - Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration.
DO  - 10.1016/j.cbi.2020.109312
SP  - 109312
ER  - 

@article{
author = "Ćupić Miladinović, Dejana and Prevendar Crnić, Andreja and Peković, Sanja and Dacić, Sanja and Ivanović, Saša and Santibanez, Juan Francisco and Ćupić, Vitomir and Borozan, Nevena and Antonijević Miljaković, Evica and Borozan, Sunčica",
year = "2020",
abstract = "Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.",
publisher = "Elsevier BV",
journal = "Chemico-Biological Interactions",
title = "Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration.",
doi = "10.1016/j.cbi.2020.109312",
pages = "109312"
}

Ćupić Miladinović, D., Prevendar Crnić, A., Peković, S., Dacić, S., Ivanović, S., Santibanez, J. F., Ćupić, V., Borozan, N., Antonijević Miljaković, E.,& Borozan, S.. (2020). Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration.. in Chemico-Biological Interactions
Elsevier BV., 109312.
https://doi.org/10.1016/j.cbi.2020.109312

Ćupić Miladinović D, Prevendar Crnić A, Peković S, Dacić S, Ivanović S, Santibanez JF, Ćupić V, Borozan N, Antonijević Miljaković E, Borozan S. Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration.. in Chemico-Biological Interactions. 2020;:109312.
doi:10.1016/j.cbi.2020.109312 .

Ćupić Miladinović, Dejana, Prevendar Crnić, Andreja, Peković, Sanja, Dacić, Sanja, Ivanović, Saša, Santibanez, Juan Francisco, Ćupić, Vitomir, Borozan, Nevena, Antonijević Miljaković, Evica, Borozan, Sunčica, "Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration." in Chemico-Biological Interactions (2020):109312,
https://doi.org/10.1016/j.cbi.2020.109312 . .