TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5140
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Oxford: Elsevier
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
SP  - 134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Oxford: Elsevier",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299",
pages = "134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Oxford: Elsevier., 402, 134299.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402:134299.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023):134299,
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6466
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
VL  - 12
DO  - 10.3390/antiox12071424
SP  - 1424
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš and Tešić, Živoslav and Stanojević, Sladjana and Pešić, Mirjana",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
volume = "12",
doi = "10.3390/antiox12071424",
pages = "1424"
}

Kostić, A., Milinčić, D., Špirović Trifunović, B., Nedić, N., Gašić, U., Tešić, Ž., Stanojević, S.,& Pešić, M.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants
Basel: MDPI., 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić A, Milinčić D, Špirović Trifunović B, Nedić N, Gašić U, Tešić Ž, Stanojević S, Pešić M. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar, Milinčić, Danijel, Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš, Tešić, Živoslav, Stanojević, Sladjana, Pešić, Mirjana, "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Petrović, Jovana
AU  - Ivanov, Marija
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6391
AB  - Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima.
AB  - The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.
PB  - Novi Sad: Institute of Field and Vegetable Crops
C3  - Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
T1  - Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima
T1  - Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6391
ER  - 

@conference{
author = "Carević, Tamara and Petrović, Jovana and Ivanov, Marija and Milinčić, Danijel and Pešić, Mirjana and Ćirić, Ana",
year = "2023",
abstract = "Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima., The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.",
publisher = "Novi Sad: Institute of Field and Vegetable Crops",
journal = "Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia",
title = "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima, Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6391"
}

Carević, T., Petrović, J., Ivanov, M., Milinčić, D., Pešić, M.,& Ćirić, A.. (2023). Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
Novi Sad: Institute of Field and Vegetable Crops., 24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391

Carević T, Petrović J, Ivanov M, Milinčić D, Pešić M, Ćirić A. Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia. 2023;:24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

Carević, Tamara, Petrović, Jovana, Ivanov, Marija, Milinčić, Danijel, Pešić, Mirjana, Ćirić, Ana, "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima" in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia (2023):24-27,
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

TY  - JOUR
AU  - Milinčić, Danijel D
AU  - Stanisavljević, Nemanja S
AU  - Kostić, Aleksandar Ž
AU  - Gašić, Uroš
AU  - Stanojević, Slađana P
AU  - Tešić, Živoslav Lj
AU  - Pešić, Mirjana B
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/11/2164
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5236
AB  - This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion
IS  - 11
VL  - 11
DO  - 10.3390/antiox11112164
SP  - 2164
ER  - 

@article{
author = "Milinčić, Danijel D and Stanisavljević, Nemanja S and Kostić, Aleksandar Ž and Gašić, Uroš and Stanojević, Slađana P and Tešić, Živoslav Lj and Pešić, Mirjana B",
year = "2022",
abstract = "This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion",
number = "11",
volume = "11",
doi = "10.3390/antiox11112164",
pages = "2164"
}

Milinčić, D. D., Stanisavljević, N. S., Kostić, A. Ž., Gašić, U., Stanojević, S. P., Tešić, Ž. L.,& Pešić, M. B.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants
Basel: MDPI., 11(11), 2164.
https://doi.org/10.3390/antiox11112164

Milinčić DD, Stanisavljević NS, Kostić AŽ, Gašić U, Stanojević SP, Tešić ŽL, Pešić MB. Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants. 2022;11(11):2164.
doi:10.3390/antiox11112164 .

Milinčić, Danijel D, Stanisavljević, Nemanja S, Kostić, Aleksandar Ž, Gašić, Uroš, Stanojević, Slađana P, Tešić, Živoslav Lj, Pešić, Mirjana B, "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion" in Antioxidants, 11, no. 11 (2022):2164,
https://doi.org/10.3390/antiox11112164 . .