TY  - JOUR
AU  - Stojanović, Ivana D.
AU  - Popadić, Dušan
AU  - Vučković, Olivera
AU  - Harhaji-Trajković, Ljubica
AU  - Miljković, Đorđe
AU  - Trajković, Vladimir
PY  - 2004
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6009
AB  - The influence of a nucleoside analog 5-aza-2'-deoxycytidine (5-AzadC) on inducible nitric oxide synthase (iNOS)-dependent nitric oxide (NO) production in various rat cell types was investigated. In C6 astrocytoma cell line and primary astrocytes, 5-AzadC enhanced proinflammatory cytokine (IFN-gamma, TNF-alpha, IL-1)-triggered NO synthesis in a time- and dose-dependent manner. In contrast, 5-AzadC did not potentiate NO production in IFN-gamma-stimulated macrophages, fibroblasts, or endothelial cells. Blockade of transcription or translation in C6 cells abolished the observed effect, suggesting the iNOS gene expression, rather than its catalytic activity, as a target for the drug action. Accordingly, 5-AzadC upregulated IFN-gamma-induced expression of iNOS mRNA in C6 astrocytes. The effect of 5-AzadC on astrocyte NO release was blocked by the inhibitor of p44/42 mitogen activated protein kinase-dependent signaling. Finally, the observed stimulatory effect of 5-AzadC on iNOS expression was apparently independent of DNA demethylation, as DNA digestion with methylation-sensitive restriction enzyme HpaII showed that 5-AzadC failed to demethylate cellular DNA in conditions used for iNOS induction.
PB  - Elsevier B.V.
T2  - Brain Research
T1  - 5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells
IS  - 1
VL  - 998
DO  - 10.1016/j.brainres.2003.11.014
SP  - 83
EP  - 90
ER  - 

@article{
author = "Stojanović, Ivana D. and Popadić, Dušan and Vučković, Olivera and Harhaji-Trajković, Ljubica and Miljković, Đorđe and Trajković, Vladimir",
year = "2004",
abstract = "The influence of a nucleoside analog 5-aza-2'-deoxycytidine (5-AzadC) on inducible nitric oxide synthase (iNOS)-dependent nitric oxide (NO) production in various rat cell types was investigated. In C6 astrocytoma cell line and primary astrocytes, 5-AzadC enhanced proinflammatory cytokine (IFN-gamma, TNF-alpha, IL-1)-triggered NO synthesis in a time- and dose-dependent manner. In contrast, 5-AzadC did not potentiate NO production in IFN-gamma-stimulated macrophages, fibroblasts, or endothelial cells. Blockade of transcription or translation in C6 cells abolished the observed effect, suggesting the iNOS gene expression, rather than its catalytic activity, as a target for the drug action. Accordingly, 5-AzadC upregulated IFN-gamma-induced expression of iNOS mRNA in C6 astrocytes. The effect of 5-AzadC on astrocyte NO release was blocked by the inhibitor of p44/42 mitogen activated protein kinase-dependent signaling. Finally, the observed stimulatory effect of 5-AzadC on iNOS expression was apparently independent of DNA demethylation, as DNA digestion with methylation-sensitive restriction enzyme HpaII showed that 5-AzadC failed to demethylate cellular DNA in conditions used for iNOS induction.",
publisher = "Elsevier B.V.",
journal = "Brain Research",
title = "5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells",
number = "1",
volume = "998",
doi = "10.1016/j.brainres.2003.11.014",
pages = "83-90"
}

Stojanović, I. D., Popadić, D., Vučković, O., Harhaji-Trajković, L., Miljković, Đ.,& Trajković, V.. (2004). 5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells. in Brain Research
Elsevier B.V.., 998(1), 83-90.
https://doi.org/10.1016/j.brainres.2003.11.014

Stojanović ID, Popadić D, Vučković O, Harhaji-Trajković L, Miljković Đ, Trajković V. 5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells. in Brain Research. 2004;998(1):83-90.
doi:10.1016/j.brainres.2003.11.014 .

Stojanović, Ivana D., Popadić, Dušan, Vučković, Olivera, Harhaji-Trajković, Ljubica, Miljković, Đorđe, Trajković, Vladimir, "5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells" in Brain Research, 998, no. 1 (2004):83-90,
https://doi.org/10.1016/j.brainres.2003.11.014 . .