TY  - JOUR
AU  - Brkljača, Nemanja
AU  - Đurović, Saša
AU  - Milošević, Sanja
AU  - Gašić, Uroš
AU  - Panković, Dejana
AU  - Zeković, Zoran
AU  - Pavlić, Branimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6469
AB  - Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towards the use of green extraction techniques and maximum utilization of raw material. In that regard conventional (hydrodistillation – HD) and novel extraction techniques (microwave-assisted hydrodistillation – MWHD, supercritical fluid extraction – SFE and microwave-assisted extraction – MAE) were applied for isolating various fractions (essential oil, lipid extracts and liquid extract) with promising bioactive potential. Essential oils and lipid extracts were characterized regarding yield, terpenoid profile determined by GC-MS and in vitro antioxidant activity towards DPPH and ABTS + radicals. Furthermore, the content of two major cannabinoids was determined in plant material and in extract obtained by SFE. Sequential SFE technique allows obtaining lipid extracts in the first step of process as well as raffinates after SFE in the second step. MAE was applied for isolation of polyphenolic fraction from raw materials and SFE raffinates. Polyphenolic content was determined by spectrophotometric assays and UHPLC-Triple-quadrupole-MS. The results suggested that hemp is an excellent source of valuable terpenoid, cannabinoid and polyphenolic antioxidant agents, and further research should be focused on their utilization in dietary supplements and functional foods.
PB  - Elsevier
T2  - Sustainable Chemistry and Pharmacy
T1  - Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds
VL  - 35
DO  - 10.1016/j.scp.2023.101213
SP  - 101213
ER  - 

@article{
author = "Brkljača, Nemanja and Đurović, Saša and Milošević, Sanja and Gašić, Uroš and Panković, Dejana and Zeković, Zoran and Pavlić, Branimir",
year = "2023",
abstract = "Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towards the use of green extraction techniques and maximum utilization of raw material. In that regard conventional (hydrodistillation – HD) and novel extraction techniques (microwave-assisted hydrodistillation – MWHD, supercritical fluid extraction – SFE and microwave-assisted extraction – MAE) were applied for isolating various fractions (essential oil, lipid extracts and liquid extract) with promising bioactive potential. Essential oils and lipid extracts were characterized regarding yield, terpenoid profile determined by GC-MS and in vitro antioxidant activity towards DPPH and ABTS + radicals. Furthermore, the content of two major cannabinoids was determined in plant material and in extract obtained by SFE. Sequential SFE technique allows obtaining lipid extracts in the first step of process as well as raffinates after SFE in the second step. MAE was applied for isolation of polyphenolic fraction from raw materials and SFE raffinates. Polyphenolic content was determined by spectrophotometric assays and UHPLC-Triple-quadrupole-MS. The results suggested that hemp is an excellent source of valuable terpenoid, cannabinoid and polyphenolic antioxidant agents, and further research should be focused on their utilization in dietary supplements and functional foods.",
publisher = "Elsevier",
journal = "Sustainable Chemistry and Pharmacy",
title = "Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds",
volume = "35",
doi = "10.1016/j.scp.2023.101213",
pages = "101213"
}

Brkljača, N., Đurović, S., Milošević, S., Gašić, U., Panković, D., Zeković, Z.,& Pavlić, B.. (2023). Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds. in Sustainable Chemistry and Pharmacy
Elsevier., 35, 101213.
https://doi.org/10.1016/j.scp.2023.101213

Brkljača N, Đurović S, Milošević S, Gašić U, Panković D, Zeković Z, Pavlić B. Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds. in Sustainable Chemistry and Pharmacy. 2023;35:101213.
doi:10.1016/j.scp.2023.101213 .

Brkljača, Nemanja, Đurović, Saša, Milošević, Sanja, Gašić, Uroš, Panković, Dejana, Zeković, Zoran, Pavlić, Branimir, "Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds" in Sustainable Chemistry and Pharmacy, 35 (2023):101213,
https://doi.org/10.1016/j.scp.2023.101213 . .

TY  - JOUR
AU  - Cvetanović Kljakić, Aleksandra
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Seebaluck-Sandoram, Roumita
AU  - Ibrahim Uba, Abdullahi
AU  - Fawzi Mahomoodally, Mohamad
AU  - Yildiztugay, Evren
AU  - Zengin, Gokhan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6470
AB  - Glaucosciadium cordifolium (Apiaceae) is traditionally used against stomach ailments, as aphrodisiac and appetizer. This study endeavored to characterize several extracts of G. cordifolium aerial parts (prepared by various isolation procedures (ASE: Accelerated solvent extraction; HAE: homogenizer-assisted extract; MAE: Microwave-assisted extraction; MAC: Maceration; SFE: supercritical CO2 extraction; SOX: Soxhlet; UAE: Ultrasound-assisted extraction) and evaluate the antioxidant activity and inhibitory activity against clinical enzymes. In order to describe the composition of the extracts liquid chromatography-mass spectrometry (LC-MS) analysis was employed. Of the total number of confirmed polyphenol components (15 in total), four were dominant in their content: 5-O-caffeoylquinic acid, p-coumaric acid, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside. Spectrophotometric analysis showed that the total phenolic and flavonoid contents differed among the extracts, which was influenced by extraction technique used for their preparation. The phenolic content of the different extracts varied in the order HAE > UAE > MAC > SOX > MAE > ASE > SFE. HAE extract yielded the highest phenolic content (49.97 mg gallic acid equivalent (GAE)/g) while SFE demonstrated the lowest phenolic content (21.65 mg GAE/g). MAC extracts contained the highest flavonoids (50.35 mg rutin equivalent (RE)/g) while SFE extract showed the lowest flavonoid content (10.87 mg RE/g). UAE, MAC, MAE, HAE, SOX and ASE showed significant DPPH antioxidant activities and the values of radical inhibitory capacity varied in the range of 55.79–54.99 mg TE/g. SFE demonstrated weak DPPH (1,1-diphenyl-2-picrylhydrazyl) radical inhibitory activity with a value 7.0 mg TE/g. UAE showed the highest ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) cation radical inhibitory potential (122.41 mg trolox equivalent (TE)/g). The CUPRAC (cupric reducing antioxidant capacity) activity of UAE was remarkable with a value of 193.58 mg TE/g. A gradual decrease in activity was observed with extracts obtained from other extraction techniques with the lowest antioxidant activity (77.0 mg TE/g) obtained from SFE extract. The highest antioxidant recorded by Phosphomolybdenum was found in HAE extract (1.62 mmol TE/g). SFE showed the highest metal chelating activity (27.30 mg ethylenediamine tetraacetic acid (EDTAE)/g). Regarding the acetylcholinesterase (AChE) inhibitory activity, MAE and ASE extracts showed the highest potential with a value of 4.06 and 4.05 mg galanthamine equivalent (GALAE)/g respectively. All tested extracts showed considerable whitening activity and SFE revealed the highest activity against tyrosinase (93.82 mg kojic acid equivalent (KAE)/g). In addition, SFE showed the best inhibitory ability against amylase (0.71 mmol acarbose equivalent (ACAE)/g). However, the extracts had similar glucosidase inhibitory effects (1.64–1.71 mmol ACAE/g). The rich chemical composition of the extracts and joint action of different classes of polyphenolic compounds in them may be responsible for their high biological activity. Extracts with the highest activity could serve in the future as an important tool for controlling the activity of clinically important enzymes and take their place in the official formulation after additional pharmacological tests.
PB  - Elsevier
T2  - Process Biochemistry
T1  - Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects
IS  - Part 1
VL  - 134
DO  - 10.1016/j.procbio.2023.10.007
SP  - 141
EP  - 150
ER  - 

@article{
author = "Cvetanović Kljakić, Aleksandra and Božunović, Jelena and Gašić, Uroš and Seebaluck-Sandoram, Roumita and Ibrahim Uba, Abdullahi and Fawzi Mahomoodally, Mohamad and Yildiztugay, Evren and Zengin, Gokhan",
year = "2023",
abstract = "Glaucosciadium cordifolium (Apiaceae) is traditionally used against stomach ailments, as aphrodisiac and appetizer. This study endeavored to characterize several extracts of G. cordifolium aerial parts (prepared by various isolation procedures (ASE: Accelerated solvent extraction; HAE: homogenizer-assisted extract; MAE: Microwave-assisted extraction; MAC: Maceration; SFE: supercritical CO2 extraction; SOX: Soxhlet; UAE: Ultrasound-assisted extraction) and evaluate the antioxidant activity and inhibitory activity against clinical enzymes. In order to describe the composition of the extracts liquid chromatography-mass spectrometry (LC-MS) analysis was employed. Of the total number of confirmed polyphenol components (15 in total), four were dominant in their content: 5-O-caffeoylquinic acid, p-coumaric acid, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside. Spectrophotometric analysis showed that the total phenolic and flavonoid contents differed among the extracts, which was influenced by extraction technique used for their preparation. The phenolic content of the different extracts varied in the order HAE > UAE > MAC > SOX > MAE > ASE > SFE. HAE extract yielded the highest phenolic content (49.97 mg gallic acid equivalent (GAE)/g) while SFE demonstrated the lowest phenolic content (21.65 mg GAE/g). MAC extracts contained the highest flavonoids (50.35 mg rutin equivalent (RE)/g) while SFE extract showed the lowest flavonoid content (10.87 mg RE/g). UAE, MAC, MAE, HAE, SOX and ASE showed significant DPPH antioxidant activities and the values of radical inhibitory capacity varied in the range of 55.79–54.99 mg TE/g. SFE demonstrated weak DPPH (1,1-diphenyl-2-picrylhydrazyl) radical inhibitory activity with a value 7.0 mg TE/g. UAE showed the highest ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) cation radical inhibitory potential (122.41 mg trolox equivalent (TE)/g). The CUPRAC (cupric reducing antioxidant capacity) activity of UAE was remarkable with a value of 193.58 mg TE/g. A gradual decrease in activity was observed with extracts obtained from other extraction techniques with the lowest antioxidant activity (77.0 mg TE/g) obtained from SFE extract. The highest antioxidant recorded by Phosphomolybdenum was found in HAE extract (1.62 mmol TE/g). SFE showed the highest metal chelating activity (27.30 mg ethylenediamine tetraacetic acid (EDTAE)/g). Regarding the acetylcholinesterase (AChE) inhibitory activity, MAE and ASE extracts showed the highest potential with a value of 4.06 and 4.05 mg galanthamine equivalent (GALAE)/g respectively. All tested extracts showed considerable whitening activity and SFE revealed the highest activity against tyrosinase (93.82 mg kojic acid equivalent (KAE)/g). In addition, SFE showed the best inhibitory ability against amylase (0.71 mmol acarbose equivalent (ACAE)/g). However, the extracts had similar glucosidase inhibitory effects (1.64–1.71 mmol ACAE/g). The rich chemical composition of the extracts and joint action of different classes of polyphenolic compounds in them may be responsible for their high biological activity. Extracts with the highest activity could serve in the future as an important tool for controlling the activity of clinically important enzymes and take their place in the official formulation after additional pharmacological tests.",
publisher = "Elsevier",
journal = "Process Biochemistry",
title = "Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects",
number = "Part 1",
volume = "134",
doi = "10.1016/j.procbio.2023.10.007",
pages = "141-150"
}

Cvetanović Kljakić, A., Božunović, J., Gašić, U., Seebaluck-Sandoram, R., Ibrahim Uba, A., Fawzi Mahomoodally, M., Yildiztugay, E.,& Zengin, G.. (2023). Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects. in Process Biochemistry
Elsevier., 134(Part 1), 141-150.
https://doi.org/10.1016/j.procbio.2023.10.007

Cvetanović Kljakić A, Božunović J, Gašić U, Seebaluck-Sandoram R, Ibrahim Uba A, Fawzi Mahomoodally M, Yildiztugay E, Zengin G. Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects. in Process Biochemistry. 2023;134(Part 1):141-150.
doi:10.1016/j.procbio.2023.10.007 .

Cvetanović Kljakić, Aleksandra, Božunović, Jelena, Gašić, Uroš, Seebaluck-Sandoram, Roumita, Ibrahim Uba, Abdullahi, Fawzi Mahomoodally, Mohamad, Yildiztugay, Evren, Zengin, Gokhan, "Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects" in Process Biochemistry, 134, no. Part 1 (2023):141-150,
https://doi.org/10.1016/j.procbio.2023.10.007 . .

TY  - JOUR
AU  - Cvetanović Kljakić, Aleksandra
AU  - Stupar, Alena
AU  - Terzić, Milena
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Yildiztugay, Evren
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6468
AB  - The plant species Opopanax hispidus is known in traditional Turkish medicine and nutrition. In addition to all the advantages that are known by the traditions of old generations, O. hispidus has been insufficiently examined, and its potential could be used in treatment in modern medicine and diet. In this paper, O. hispidus extracts obtained by conventional (maceration (MAC), and Soxhlet (SOX)) and contemporary (accelerated solvent extraction (ASE), homogenizer assisted extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SFE)) methods were examined in terms of their chemical composition and biological potential. The extract obtained by HAE extraction had the highest content of total phenolics and flavonoids (36.98 mg GAE/g and 15.04 mg RE/g, respectively). The highest antioxidant activity was recorded for HAE and ASE extracts. O. hispidus extracts achieved inhibitory activity against acetylcholinesterase (4.12–6.44 mg GALAE/g), tyrosinase (44.78–76.72 mg KAE/g), α-amylase (0.35–0.46 mmol ACAE/g), and α-glucosidase (1.67–1.77 mmol ACAE/g) enzymes. LC-MS technique was used for the quantitative analysis of the extracts, allowing the quantification of chlorogenic acid, neochlorogenic acid, and p-coumaric acid, rutin, luteolin, and kaempferol 3-O-glucoside as the most abundant phenolic components. Results suggested that the green extraction techniques for obtaining extracts that are rich in bioactive compounds have advantages compared to conventional extraction. Therefore, these extracts can be used as antioxidants, neuroprotective, antityrosinase, and antidiabetic agents while respecting the principles of sustainable development and green chemistry.
PB  - Elsevier
T2  - Sustainable Chemistry and Pharmacy
T1  - Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies
VL  - 33
DO  - 10.1016/j.scp.2023.101122
SP  - 101122
ER  - 

@article{
author = "Cvetanović Kljakić, Aleksandra and Stupar, Alena and Terzić, Milena and Božunović, Jelena and Gašić, Uroš and Zengin, Gokhan and Yildiztugay, Evren",
year = "2023",
abstract = "The plant species Opopanax hispidus is known in traditional Turkish medicine and nutrition. In addition to all the advantages that are known by the traditions of old generations, O. hispidus has been insufficiently examined, and its potential could be used in treatment in modern medicine and diet. In this paper, O. hispidus extracts obtained by conventional (maceration (MAC), and Soxhlet (SOX)) and contemporary (accelerated solvent extraction (ASE), homogenizer assisted extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SFE)) methods were examined in terms of their chemical composition and biological potential. The extract obtained by HAE extraction had the highest content of total phenolics and flavonoids (36.98 mg GAE/g and 15.04 mg RE/g, respectively). The highest antioxidant activity was recorded for HAE and ASE extracts. O. hispidus extracts achieved inhibitory activity against acetylcholinesterase (4.12–6.44 mg GALAE/g), tyrosinase (44.78–76.72 mg KAE/g), α-amylase (0.35–0.46 mmol ACAE/g), and α-glucosidase (1.67–1.77 mmol ACAE/g) enzymes. LC-MS technique was used for the quantitative analysis of the extracts, allowing the quantification of chlorogenic acid, neochlorogenic acid, and p-coumaric acid, rutin, luteolin, and kaempferol 3-O-glucoside as the most abundant phenolic components. Results suggested that the green extraction techniques for obtaining extracts that are rich in bioactive compounds have advantages compared to conventional extraction. Therefore, these extracts can be used as antioxidants, neuroprotective, antityrosinase, and antidiabetic agents while respecting the principles of sustainable development and green chemistry.",
publisher = "Elsevier",
journal = "Sustainable Chemistry and Pharmacy",
title = "Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies",
volume = "33",
doi = "10.1016/j.scp.2023.101122",
pages = "101122"
}

Cvetanović Kljakić, A., Stupar, A., Terzić, M., Božunović, J., Gašić, U., Zengin, G.,& Yildiztugay, E.. (2023). Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies. in Sustainable Chemistry and Pharmacy
Elsevier., 33, 101122.
https://doi.org/10.1016/j.scp.2023.101122

Cvetanović Kljakić A, Stupar A, Terzić M, Božunović J, Gašić U, Zengin G, Yildiztugay E. Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies. in Sustainable Chemistry and Pharmacy. 2023;33:101122.
doi:10.1016/j.scp.2023.101122 .

Cvetanović Kljakić, Aleksandra, Stupar, Alena, Terzić, Milena, Božunović, Jelena, Gašić, Uroš, Zengin, Gokhan, Yildiztugay, Evren, "Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies" in Sustainable Chemistry and Pharmacy, 33 (2023):101122,
https://doi.org/10.1016/j.scp.2023.101122 . .