TY  - JOUR
AU  - Umhang, Gérald
AU  - Bastid, Vanessa
AU  - Avcioglu, Hamza
AU  - Bagrade, Guna
AU  - Bujanić, Miljenko
AU  - Bjelić Čabrilo, Oliveira
AU  - Casulli, Adriano
AU  - Dorny, Pierre
AU  - van der Giessen, Joke
AU  - Guven, Esin
AU  - Harna, Jiri
AU  - Karamon, Jacek
AU  - Kharchenko, Vitaliy
AU  - Knapp, Jenny
AU  - Kolarova, Libuse
AU  - Konyaev, Sergey
AU  - Laurimaa, Leidi
AU  - Losch, Serge
AU  - Miljević, Milan
AU  - Miterpakova, Martina
AU  - Moks, Epp
AU  - Romig, Thomas
AU  - Saarma, Urmas
AU  - Snabel, Viliam
AU  - Sreter, Tamas
AU  - Valdmann, Harri
AU  - Boué, Franck
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4225
AB  - The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic data from most eastern European countries is a major knowledge gap, affecting the study of associations with parasite populations in Western Europe. In this study, EmsB microsatellite genotyping of E. multilocularis was performed to describe the genetic diversity and relatedness of 785 E. multilocularis isolates from four western and nine eastern European countries, as well as from Armenia and the Asian parts of Russia and Turkey. The presence of the same E. multilocularis populations in the Benelux resulting from expansion from the historical Alpine focus can be deduced from the main profiles shared between these countries. All 33 EmsB profiles obtained from 528 samples from the nine eastern European countries belonged to the European clade, except one Asian profile form Ryazan Oblast, Russia. The expansion of E. multilocularis seems to have progressed from the historical Alpine focus through Hungary, Slovakia, the Czech Republic and southern Poland towards Latvia and Estonia. Most of the samples from Asia belong to the Asian clade, with one EmsB profile shared between Armenia and Turkey, and two between Turkey and Russia. However, two European profiles were described from two foxes in Turkey, including one harboring worms from both European and Asian clades. Three EmsB profiles from three Russian samples were associated with the Arctic clade. Two E. multilocularis profiles from rodents from Lake Baikal belonged to the Mongolian clade, described for the first time here using EmsB. Further worldwide studies on the genetic diversity of E. multilocularis using both mitochondrial sequencing and EmsB genotyping are needed to understand the distribution and expansion of the various clades.
PB  - Elsevier B.V.
T2  - Infection, Genetics and Evolution
T1  - Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
VL  - 92
DO  - 10.1016/j.meegid.2021.104863
SP  - 104863
ER  - 

@article{
author = "Umhang, Gérald and Bastid, Vanessa and Avcioglu, Hamza and Bagrade, Guna and Bujanić, Miljenko and Bjelić Čabrilo, Oliveira and Casulli, Adriano and Dorny, Pierre and van der Giessen, Joke and Guven, Esin and Harna, Jiri and Karamon, Jacek and Kharchenko, Vitaliy and Knapp, Jenny and Kolarova, Libuse and Konyaev, Sergey and Laurimaa, Leidi and Losch, Serge and Miljević, Milan and Miterpakova, Martina and Moks, Epp and Romig, Thomas and Saarma, Urmas and Snabel, Viliam and Sreter, Tamas and Valdmann, Harri and Boué, Franck",
year = "2021",
abstract = "The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic data from most eastern European countries is a major knowledge gap, affecting the study of associations with parasite populations in Western Europe. In this study, EmsB microsatellite genotyping of E. multilocularis was performed to describe the genetic diversity and relatedness of 785 E. multilocularis isolates from four western and nine eastern European countries, as well as from Armenia and the Asian parts of Russia and Turkey. The presence of the same E. multilocularis populations in the Benelux resulting from expansion from the historical Alpine focus can be deduced from the main profiles shared between these countries. All 33 EmsB profiles obtained from 528 samples from the nine eastern European countries belonged to the European clade, except one Asian profile form Ryazan Oblast, Russia. The expansion of E. multilocularis seems to have progressed from the historical Alpine focus through Hungary, Slovakia, the Czech Republic and southern Poland towards Latvia and Estonia. Most of the samples from Asia belong to the Asian clade, with one EmsB profile shared between Armenia and Turkey, and two between Turkey and Russia. However, two European profiles were described from two foxes in Turkey, including one harboring worms from both European and Asian clades. Three EmsB profiles from three Russian samples were associated with the Arctic clade. Two E. multilocularis profiles from rodents from Lake Baikal belonged to the Mongolian clade, described for the first time here using EmsB. Further worldwide studies on the genetic diversity of E. multilocularis using both mitochondrial sequencing and EmsB genotyping are needed to understand the distribution and expansion of the various clades.",
publisher = "Elsevier B.V.",
journal = "Infection, Genetics and Evolution",
title = "Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker",
volume = "92",
doi = "10.1016/j.meegid.2021.104863",
pages = "104863"
}

Umhang, G., Bastid, V., Avcioglu, H., Bagrade, G., Bujanić, M., Bjelić Čabrilo, O., Casulli, A., Dorny, P., van der Giessen, J., Guven, E., Harna, J., Karamon, J., Kharchenko, V., Knapp, J., Kolarova, L., Konyaev, S., Laurimaa, L., Losch, S., Miljević, M., Miterpakova, M., Moks, E., Romig, T., Saarma, U., Snabel, V., Sreter, T., Valdmann, H.,& Boué, F.. (2021). Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker. in Infection, Genetics and Evolution
Elsevier B.V.., 92, 104863.
https://doi.org/10.1016/j.meegid.2021.104863

Umhang G, Bastid V, Avcioglu H, Bagrade G, Bujanić M, Bjelić Čabrilo O, Casulli A, Dorny P, van der Giessen J, Guven E, Harna J, Karamon J, Kharchenko V, Knapp J, Kolarova L, Konyaev S, Laurimaa L, Losch S, Miljević M, Miterpakova M, Moks E, Romig T, Saarma U, Snabel V, Sreter T, Valdmann H, Boué F. Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker. in Infection, Genetics and Evolution. 2021;92:104863.
doi:10.1016/j.meegid.2021.104863 .

Umhang, Gérald, Bastid, Vanessa, Avcioglu, Hamza, Bagrade, Guna, Bujanić, Miljenko, Bjelić Čabrilo, Oliveira, Casulli, Adriano, Dorny, Pierre, van der Giessen, Joke, Guven, Esin, Harna, Jiri, Karamon, Jacek, Kharchenko, Vitaliy, Knapp, Jenny, Kolarova, Libuse, Konyaev, Sergey, Laurimaa, Leidi, Losch, Serge, Miljević, Milan, Miterpakova, Martina, Moks, Epp, Romig, Thomas, Saarma, Urmas, Snabel, Viliam, Sreter, Tamas, Valdmann, Harri, Boué, Franck, "Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker" in Infection, Genetics and Evolution, 92 (2021):104863,
https://doi.org/10.1016/j.meegid.2021.104863 . .

TY  - JOUR
AU  - Tokodi, Nada
AU  - Drobac, Damjana
AU  - Meriluoto, Jussi
AU  - Lujić, Jelena
AU  - Marinović, Zoran
AU  - Važić, Tamara
AU  - Nybom, Sonja
AU  - Simeunović, Jelica
AU  - Dulić, Tamara
AU  - Lazić, Gospava
AU  - Petrović, Tamaš
AU  - Vuković-Gačić, Branka
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Subakov-Simić, Gordana
AU  - Miljanović, Branko
AU  - Codd, Geoffrey A.
AU  - Svirčev, Zorica
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969718313512
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29710560
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3046
AB  - Cyanobacteria are present in many aquatic ecosystems in Serbia. Lake Ludoš, a wetland area of international significance and an important habitat for waterbirds, has become the subject of intense research interest because of practically continuous blooming of cyanobacteria. Analyses of water samples indicated a deterioration of ecological condition and water quality, and the presence of toxin-producing cyanobacteria (the most abundant Limnothrix redekei, Pseudanabaena limnetica, Planktothrix agardhii and Microcystis spp.). Furthermore, microcystins were detected in plants and animals from the lake: in macrophyte rhizomes (Phragmites communis, Typha latifolia and Nymphaea elegans), and in the muscle, intestines, kidneys, gonads and gills of fish (Carassius gibelio). Moreover, histopathological deleterious effects (liver, kidney, gills and intestines) and DNA damage (liver and gills) were observed in fish. A potential treatment for the reduction of cyanobacterial populations employing hydrogen peroxide was tested during this study. The treatment was not effective in laboratory tests although further in-lake trials are needed to make final conclusions about the applicability of the method. Based on our observations of the cyanobacterial populations and cyanotoxins in the water, as well as other aquatic organisms and, a survey of historical data on Lake Ludoš, it can be concluded that the lake is continuously in a poor ecological state. Conservation of the lake in order to protect the waterbirds (without urgent control of eutrophication) actually endangers them and the rest of the biota in this wetland habitat, and possibly other ecosystems. Thus, urgent measures for restoration are required, so that the preservation of this Ramsar site would be meaningful.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?
VL  - 635
DO  - 10.1016/j.scitotenv.2018.04.177
SP  - 1047
EP  - 1062
ER  - 

@article{
author = "Tokodi, Nada and Drobac, Damjana and Meriluoto, Jussi and Lujić, Jelena and Marinović, Zoran and Važić, Tamara and Nybom, Sonja and Simeunović, Jelica and Dulić, Tamara and Lazić, Gospava and Petrović, Tamaš and Vuković-Gačić, Branka and Sunjog, Karolina and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Subakov-Simić, Gordana and Miljanović, Branko and Codd, Geoffrey A. and Svirčev, Zorica",
year = "2018",
abstract = "Cyanobacteria are present in many aquatic ecosystems in Serbia. Lake Ludoš, a wetland area of international significance and an important habitat for waterbirds, has become the subject of intense research interest because of practically continuous blooming of cyanobacteria. Analyses of water samples indicated a deterioration of ecological condition and water quality, and the presence of toxin-producing cyanobacteria (the most abundant Limnothrix redekei, Pseudanabaena limnetica, Planktothrix agardhii and Microcystis spp.). Furthermore, microcystins were detected in plants and animals from the lake: in macrophyte rhizomes (Phragmites communis, Typha latifolia and Nymphaea elegans), and in the muscle, intestines, kidneys, gonads and gills of fish (Carassius gibelio). Moreover, histopathological deleterious effects (liver, kidney, gills and intestines) and DNA damage (liver and gills) were observed in fish. A potential treatment for the reduction of cyanobacterial populations employing hydrogen peroxide was tested during this study. The treatment was not effective in laboratory tests although further in-lake trials are needed to make final conclusions about the applicability of the method. Based on our observations of the cyanobacterial populations and cyanotoxins in the water, as well as other aquatic organisms and, a survey of historical data on Lake Ludoš, it can be concluded that the lake is continuously in a poor ecological state. Conservation of the lake in order to protect the waterbirds (without urgent control of eutrophication) actually endangers them and the rest of the biota in this wetland habitat, and possibly other ecosystems. Thus, urgent measures for restoration are required, so that the preservation of this Ramsar site would be meaningful.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?",
volume = "635",
doi = "10.1016/j.scitotenv.2018.04.177",
pages = "1047-1062"
}

Tokodi, N., Drobac, D., Meriluoto, J., Lujić, J., Marinović, Z., Važić, T., Nybom, S., Simeunović, J., Dulić, T., Lazić, G., Petrović, T., Vuković-Gačić, B., Sunjog, K., Kolarević, S., Kračun-Kolarević, M., Subakov-Simić, G., Miljanović, B., Codd, G. A.,& Svirčev, Z.. (2018). Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?. in Science of the Total Environment
Elsevier B.V.., 635, 1047-1062.
https://doi.org/10.1016/j.scitotenv.2018.04.177

Tokodi N, Drobac D, Meriluoto J, Lujić J, Marinović Z, Važić T, Nybom S, Simeunović J, Dulić T, Lazić G, Petrović T, Vuković-Gačić B, Sunjog K, Kolarević S, Kračun-Kolarević M, Subakov-Simić G, Miljanović B, Codd GA, Svirčev Z. Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?. in Science of the Total Environment. 2018;635:1047-1062.
doi:10.1016/j.scitotenv.2018.04.177 .

Tokodi, Nada, Drobac, Damjana, Meriluoto, Jussi, Lujić, Jelena, Marinović, Zoran, Važić, Tamara, Nybom, Sonja, Simeunović, Jelica, Dulić, Tamara, Lazić, Gospava, Petrović, Tamaš, Vuković-Gačić, Branka, Sunjog, Karolina, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Subakov-Simić, Gordana, Miljanović, Branko, Codd, Geoffrey A., Svirčev, Zorica, "Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?" in Science of the Total Environment, 635 (2018):1047-1062,
https://doi.org/10.1016/j.scitotenv.2018.04.177 . .