TY  - JOUR
AU  - Jevtić, Ivana I.
AU  - Suručić, Relja V.
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Kostić-Rajačić, Slađana V.
AU  - Andrić, Deana
AU  - Penjišević, Jelena Z.
PY  - 2024
UR  - https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4662547
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6648
AB  - Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T2  - Bioorganic & Medicinal Chemistry
T1  - Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study
VL  - 101
DO  - 10.1016/j.bmc.2024.117649
SP  - 117649
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6648
ER  - 

@article{
author = "Jevtić, Ivana I. and Suručić, Relja V. and Tovilović-Kovačević, Gordana and Zogović, Nevena and Kostić-Rajačić, Slađana V. and Andrić, Deana and Penjišević, Jelena Z.",
year = "2024",
abstract = "Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Bioorganic & Medicinal Chemistry",
title = "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study",
volume = "101",
doi = "10.1016/j.bmc.2024.117649",
pages = "117649",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6648"
}

Jevtić, I. I., Suručić, R. V., Tovilović-Kovačević, G., Zogović, N., Kostić-Rajačić, S. V., Andrić, D.,& Penjišević, J. Z.. (2024). Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 101, 117649.
https://doi.org/10.1016/j.bmc.2024.117649
https://hdl.handle.net/21.15107/rcub_ibiss_6648

Jevtić II, Suručić RV, Tovilović-Kovačević G, Zogović N, Kostić-Rajačić SV, Andrić D, Penjišević JZ. Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry. 2024;101:117649.
doi:10.1016/j.bmc.2024.117649
https://hdl.handle.net/21.15107/rcub_ibiss_6648 .

Jevtić, Ivana I., Suručić, Relja V., Tovilović-Kovačević, Gordana, Zogović, Nevena, Kostić-Rajačić, Slađana V., Andrić, Deana, Penjišević, Jelena Z., "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study" in Bioorganic & Medicinal Chemistry, 101 (2024):117649,
https://doi.org/10.1016/j.bmc.2024.117649 .,
https://hdl.handle.net/21.15107/rcub_ibiss_6648 .

TY  - JOUR
AU  - Jevtić, Ivana I.
AU  - Suručić, Relja V.
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Kostić-Rajačić, Slađana V.
AU  - Andrić, Deana
AU  - Penjišević, Jelena Z.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6629
UR  - https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4662547
AB  - Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.
PB  - Amsterdam: Elsevier Ltd
T2  - Bioorganic & Medicinal Chemistry
T1  - Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study
VL  - 101
DO  - 10.1016/j.bmc.2024.117649
SP  - 117649
ER  - 

@article{
author = "Jevtić, Ivana I. and Suručić, Relja V. and Tovilović-Kovačević, Gordana and Zogović, Nevena and Kostić-Rajačić, Slađana V. and Andrić, Deana and Penjišević, Jelena Z.",
year = "2024",
abstract = "Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.",
publisher = "Amsterdam: Elsevier Ltd",
journal = "Bioorganic & Medicinal Chemistry",
title = "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study",
volume = "101",
doi = "10.1016/j.bmc.2024.117649",
pages = "117649"
}

Jevtić, I. I., Suručić, R. V., Tovilović-Kovačević, G., Zogović, N., Kostić-Rajačić, S. V., Andrić, D.,& Penjišević, J. Z.. (2024). Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry
Amsterdam: Elsevier Ltd., 101, 117649.
https://doi.org/10.1016/j.bmc.2024.117649

Jevtić II, Suručić RV, Tovilović-Kovačević G, Zogović N, Kostić-Rajačić SV, Andrić D, Penjišević JZ. Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry. 2024;101:117649.
doi:10.1016/j.bmc.2024.117649 .

Jevtić, Ivana I., Suručić, Relja V., Tovilović-Kovačević, Gordana, Zogović, Nevena, Kostić-Rajačić, Slađana V., Andrić, Deana, Penjišević, Jelena Z., "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study" in Bioorganic & Medicinal Chemistry, 101 (2024):117649,
https://doi.org/10.1016/j.bmc.2024.117649 . .

TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Krstić, Gordana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6557
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - Basel: MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
IS  - 24
VL  - 12
DO  - 10.3390/plants12244181
SP  - 4181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan and Krstić, Gordana",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
number = "24",
volume = "12",
doi = "10.3390/plants12244181",
pages = "4181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S.,& Krstić, G.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
Basel: MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević S, Krstić G. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, Krstić, Gordana, "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6467
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
SP  - 477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477",
pages = "477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae
Basel: MDPI., 9(4), 477.
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4):477.
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023):477,
https://doi.org/10.3390/horticulturae9040477 . .

TY  - JOUR
AU  - Ljubić, Verica
AU  - Perendija, Jovana
AU  - Cvetković, Slobodan
AU  - Rogan, Jelena
AU  - Trivunac, Katarina
AU  - Stojanović, Marijana
AU  - Popović, Mina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6402
AB  - Nowadays, exopolysaccharides (EPS) produced from bacterial cells are manufactured for their use in different industries in the world, mainly in the food, pharmaceutical, and wastewater industries. The characteristics of EPS, such as being biodegradable, safe, high adsorption capacity, and reusable, make them significant and potential applications in the purification of contaminated water of heavy metals. In this study, the possible application in biosorption Ni2+ ions from contaminated water was assessed using this exopolysaccharide as a biosorbent. The new exopolysaccharide from the bacterial strain K. oxytoca J7 was extracted, isolated, and characterized using SEM, FTIR, XRD, TGA/DTG, and MALDI-TOF MS analysis. Likewise, the cytotoxic activity was performed for EPS from K. oxytoca J7 strain. The aim of this study was to investigate the possible application of non-toxic exopolysaccharide in the purification of contaminated water by removing Ni2+ ions. The results obtained from the biosorption study showed that the Langmuir model is well suited to describe the adsorption process of Ni2+ ions by EPS from K. oxytoca J7, with a maximum adsorption capacity of 269.97 mg g–1. The importance of this study is the possible use of natural nontoxic exopolysaccharide extracted from the pathogen microorganism, K. oxytoca J7, for the removal of Ni2+ ions from the contaminated water.
PB  - New York: Springer
T2  - Journal of Polymers and the Environment
T1  - Removal of Ni2+ ions from Contaminated Water by New Exopolysaccharide Extracted from K. oxytoca J7 as Biosorbent
DO  - 10.1007/s10924-023-03031-5
ER  - 

@article{
author = "Ljubić, Verica and Perendija, Jovana and Cvetković, Slobodan and Rogan, Jelena and Trivunac, Katarina and Stojanović, Marijana and Popović, Mina",
year = "2023",
abstract = "Nowadays, exopolysaccharides (EPS) produced from bacterial cells are manufactured for their use in different industries in the world, mainly in the food, pharmaceutical, and wastewater industries. The characteristics of EPS, such as being biodegradable, safe, high adsorption capacity, and reusable, make them significant and potential applications in the purification of contaminated water of heavy metals. In this study, the possible application in biosorption Ni2+ ions from contaminated water was assessed using this exopolysaccharide as a biosorbent. The new exopolysaccharide from the bacterial strain K. oxytoca J7 was extracted, isolated, and characterized using SEM, FTIR, XRD, TGA/DTG, and MALDI-TOF MS analysis. Likewise, the cytotoxic activity was performed for EPS from K. oxytoca J7 strain. The aim of this study was to investigate the possible application of non-toxic exopolysaccharide in the purification of contaminated water by removing Ni2+ ions. The results obtained from the biosorption study showed that the Langmuir model is well suited to describe the adsorption process of Ni2+ ions by EPS from K. oxytoca J7, with a maximum adsorption capacity of 269.97 mg g–1. The importance of this study is the possible use of natural nontoxic exopolysaccharide extracted from the pathogen microorganism, K. oxytoca J7, for the removal of Ni2+ ions from the contaminated water.",
publisher = "New York: Springer",
journal = "Journal of Polymers and the Environment",
title = "Removal of Ni2+ ions from Contaminated Water by New Exopolysaccharide Extracted from K. oxytoca J7 as Biosorbent",
doi = "10.1007/s10924-023-03031-5"
}

Ljubić, V., Perendija, J., Cvetković, S., Rogan, J., Trivunac, K., Stojanović, M.,& Popović, M.. (2023). Removal of Ni2+ ions from Contaminated Water by New Exopolysaccharide Extracted from K. oxytoca J7 as Biosorbent. in Journal of Polymers and the Environment
New York: Springer..
https://doi.org/10.1007/s10924-023-03031-5

Ljubić V, Perendija J, Cvetković S, Rogan J, Trivunac K, Stojanović M, Popović M. Removal of Ni2+ ions from Contaminated Water by New Exopolysaccharide Extracted from K. oxytoca J7 as Biosorbent. in Journal of Polymers and the Environment. 2023;.
doi:10.1007/s10924-023-03031-5 .

Ljubić, Verica, Perendija, Jovana, Cvetković, Slobodan, Rogan, Jelena, Trivunac, Katarina, Stojanović, Marijana, Popović, Mina, "Removal of Ni2+ ions from Contaminated Water by New Exopolysaccharide Extracted from K. oxytoca J7 as Biosorbent" in Journal of Polymers and the Environment (2023),
https://doi.org/10.1007/s10924-023-03031-5 . .

TY  - CONF
AU  - Pergal, Marija V.
AU  - Brkljačić, Jelena
AU  - Vasiljević Radović, Dana
AU  - Pergal, Miodrag M.
AU  - Pešić, Ivan
AU  - Dević, Gordana
AU  - Tovilović-Kovačević, Gordana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6192
AB  - Polyurethane (PU) nanocomposite materials, offer very desirable advantages over pure PU materials,as the nanocomposites have enhanced thermal, surface, mechanical and biological properties. The main goal of this study was to develop a new kind of novel nanocomposites consisting of crosslinked PUs (based on poly(dimetylsiloxane) and hyperbranched polyester) and ferrite nanoparticles (based on copper and zinc) for possible application as coatings on biomedical devices and implants. A series of PU/ferrite nanocomposites was prepared by in situ polymerization in solution. Characterization of prepared nanocomposites nanocomposites was conducted by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Copper and zinc releases were investigated by microwave plasma atomic emission spectrometry (MP-AES). Characteristics of the prepared nanocomposites when in contact with a biological environment were examined through testing their biocompatibility, and adhesion of fibroblast cells. The presence of the nanoferrite nanoparticles influenced on surface and biological properties of PU nanocomposites. The prepared PU nanocomposites with noncytotoxic chemistry could be used as promising materials for vascular implants development.
PB  - Belgrade: Serbian Ceramic Society
C3  - Program and the Book of Abstracts: Serbian Ceramic Society Conference Advanced Ceramics and Application 11: New Frontiers in Multifunctional Material Science and Processing; 2023 Sep 18-20; Belgrade, Serbia
T1  - Characterization of polyurethane/ferrite nanocomposites
SP  - 65
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6192
ER  - 

@conference{
author = "Pergal, Marija V. and Brkljačić, Jelena and Vasiljević Radović, Dana and Pergal, Miodrag M. and Pešić, Ivan and Dević, Gordana and Tovilović-Kovačević, Gordana",
year = "2023",
abstract = "Polyurethane (PU) nanocomposite materials, offer very desirable advantages over pure PU materials,as the nanocomposites have enhanced thermal, surface, mechanical and biological properties. The main goal of this study was to develop a new kind of novel nanocomposites consisting of crosslinked PUs (based on poly(dimetylsiloxane) and hyperbranched polyester) and ferrite nanoparticles (based on copper and zinc) for possible application as coatings on biomedical devices and implants. A series of PU/ferrite nanocomposites was prepared by in situ polymerization in solution. Characterization of prepared nanocomposites nanocomposites was conducted by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Copper and zinc releases were investigated by microwave plasma atomic emission spectrometry (MP-AES). Characteristics of the prepared nanocomposites when in contact with a biological environment were examined through testing their biocompatibility, and adhesion of fibroblast cells. The presence of the nanoferrite nanoparticles influenced on surface and biological properties of PU nanocomposites. The prepared PU nanocomposites with noncytotoxic chemistry could be used as promising materials for vascular implants development.",
publisher = "Belgrade: Serbian Ceramic Society",
journal = "Program and the Book of Abstracts: Serbian Ceramic Society Conference Advanced Ceramics and Application 11: New Frontiers in Multifunctional Material Science and Processing; 2023 Sep 18-20; Belgrade, Serbia",
title = "Characterization of polyurethane/ferrite nanocomposites",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6192"
}

Pergal, M. V., Brkljačić, J., Vasiljević Radović, D., Pergal, M. M., Pešić, I., Dević, G.,& Tovilović-Kovačević, G.. (2023). Characterization of polyurethane/ferrite nanocomposites. in Program and the Book of Abstracts: Serbian Ceramic Society Conference Advanced Ceramics and Application 11: New Frontiers in Multifunctional Material Science and Processing; 2023 Sep 18-20; Belgrade, Serbia
Belgrade: Serbian Ceramic Society., 65-65.
https://hdl.handle.net/21.15107/rcub_ibiss_6192

Pergal MV, Brkljačić J, Vasiljević Radović D, Pergal MM, Pešić I, Dević G, Tovilović-Kovačević G. Characterization of polyurethane/ferrite nanocomposites. in Program and the Book of Abstracts: Serbian Ceramic Society Conference Advanced Ceramics and Application 11: New Frontiers in Multifunctional Material Science and Processing; 2023 Sep 18-20; Belgrade, Serbia. 2023;:65-65.
https://hdl.handle.net/21.15107/rcub_ibiss_6192 .

Pergal, Marija V., Brkljačić, Jelena, Vasiljević Radović, Dana, Pergal, Miodrag M., Pešić, Ivan, Dević, Gordana, Tovilović-Kovačević, Gordana, "Characterization of polyurethane/ferrite nanocomposites" in Program and the Book of Abstracts: Serbian Ceramic Society Conference Advanced Ceramics and Application 11: New Frontiers in Multifunctional Material Science and Processing; 2023 Sep 18-20; Belgrade, Serbia (2023):65-65,
https://hdl.handle.net/21.15107/rcub_ibiss_6192 .

TY  - CONF
AU  - Pergal, Marija
AU  - Brkljačić, Jelena
AU  - Pešić, Ivan
AU  - Dević, Gordana
AU  - Dojčinović, Biljana P.
AU  - Antić, Bratislav
AU  - Tovilović-Kovačević, Gordana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6191
AB  - Polyurethane (PU) and PU nanocomposites with good biocompatibility and mechanical properties can be used as the biomedical matrix and tissue engineering biomaterials. Magnetic nanoparticles, especially ferrite nanoparticles have attracted much interest due to their specific physicochemical properties in various areas including magnetic recording, biosensing, catalyst, drug delivery systems, magnetic resonance imaging (MRI) and cancer therapy. Despite all these advantages, the nanoparticle agglomeration reduces the efficiency of the nanoparticles, so the nanoparticle incorporation into an appropriate polymeric matrix to prepare organic-inorganic nanocomposites is a right direction in the current scenario of biomedical nanotechnology. In this study, organic-inorganic PU nanocomposites based on zinc and copper ferrites and with the same composition of PU were prepared. The properties of PU nanocomposites were evaluated by nanoindentation, water contact angle and water absorption measurements. The presence of the nanoferrite nanoparticles affects properties of PU nanocomposites such as bulk morphology, mechanical, and biological properties. The biocompatibility of PU nanocomposites was investigated by MTT assay and cell attachment using endothelial cells. According to the results, the prepared PU nanocomposites with noncytotoxic chemistry could be a potential choice for vascular implants development.
PB  - Niš, Serbia: RAD Centre
C3  - Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro
T1  - Organic-inorganic nanocomposites for biomedical applications
DO  - 10.21175/rad.abstr.book.2023.19.20
SP  - 99
EP  - 99
ER  - 

@conference{
author = "Pergal, Marija and Brkljačić, Jelena and Pešić, Ivan and Dević, Gordana and Dojčinović, Biljana P. and Antić, Bratislav and Tovilović-Kovačević, Gordana",
year = "2023",
abstract = "Polyurethane (PU) and PU nanocomposites with good biocompatibility and mechanical properties can be used as the biomedical matrix and tissue engineering biomaterials. Magnetic nanoparticles, especially ferrite nanoparticles have attracted much interest due to their specific physicochemical properties in various areas including magnetic recording, biosensing, catalyst, drug delivery systems, magnetic resonance imaging (MRI) and cancer therapy. Despite all these advantages, the nanoparticle agglomeration reduces the efficiency of the nanoparticles, so the nanoparticle incorporation into an appropriate polymeric matrix to prepare organic-inorganic nanocomposites is a right direction in the current scenario of biomedical nanotechnology. In this study, organic-inorganic PU nanocomposites based on zinc and copper ferrites and with the same composition of PU were prepared. The properties of PU nanocomposites were evaluated by nanoindentation, water contact angle and water absorption measurements. The presence of the nanoferrite nanoparticles affects properties of PU nanocomposites such as bulk morphology, mechanical, and biological properties. The biocompatibility of PU nanocomposites was investigated by MTT assay and cell attachment using endothelial cells. According to the results, the prepared PU nanocomposites with noncytotoxic chemistry could be a potential choice for vascular implants development.",
publisher = "Niš, Serbia: RAD Centre",
journal = "Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro",
title = "Organic-inorganic nanocomposites for biomedical applications",
doi = "10.21175/rad.abstr.book.2023.19.20",
pages = "99-99"
}

Pergal, M., Brkljačić, J., Pešić, I., Dević, G., Dojčinović, B. P., Antić, B.,& Tovilović-Kovačević, G.. (2023). Organic-inorganic nanocomposites for biomedical applications. in Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro
Niš, Serbia: RAD Centre., 99-99.
https://doi.org/10.21175/rad.abstr.book.2023.19.20

Pergal M, Brkljačić J, Pešić I, Dević G, Dojčinović BP, Antić B, Tovilović-Kovačević G. Organic-inorganic nanocomposites for biomedical applications. in Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro. 2023;:99-99.
doi:10.21175/rad.abstr.book.2023.19.20 .

Pergal, Marija, Brkljačić, Jelena, Pešić, Ivan, Dević, Gordana, Dojčinović, Biljana P., Antić, Bratislav, Tovilović-Kovačević, Gordana, "Organic-inorganic nanocomposites for biomedical applications" in Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro (2023):99-99,
https://doi.org/10.21175/rad.abstr.book.2023.19.20 . .

TY  - JOUR
AU  - Platanić-Arizanović, Lena
AU  - Gligorijević, Nikola
AU  - Cvijetić, Ilija
AU  - Mijatović, Aleksandar
AU  - Krstić-Ristivojević, Maja
AU  - Minić, Simeon
AU  - Nikolić-Kokić, Aleksandra
AU  - Miljević, Čedo
AU  - Nikolić, Milan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6028
AB  - : Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes
are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investigated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human
hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching
at different temperatures and data obtained from the van’t Hoff diagram and molecular docking
indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site
for all drugs in the central cavity near αβ interfaces and is dominantly mediated through hydrophobic
forces. The association constants were lower-moderate strength (~104 M−1
), the highest observed
for clozapine (2.2 × 104 M−1 at 25 ◦C). The clozapine binding showed “friendly” effects: increased
α-helical content, a higher melting point, and protein protection from free radical-mediated oxidation.
On the other hand, bound ziprasidone and sertindole had a slightly pro-oxidative effect, increasing
ferrihemoglobin content, a possible “foe”. Since the interaction of proteins with drugs plays a vital
role in their pharmacokinetic and pharmacodynamic properties, the physiological significance of the
obtained findings is briefly discussed.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?
IS  - 10
VL  - 24
DO  - 10.3390/ijms24108921
SP  - 8921
ER  - 

@article{
author = "Platanić-Arizanović, Lena and Gligorijević, Nikola and Cvijetić, Ilija and Mijatović, Aleksandar and Krstić-Ristivojević, Maja and Minić, Simeon and Nikolić-Kokić, Aleksandra and Miljević, Čedo and Nikolić, Milan",
year = "2023",
abstract = ": Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes
are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investigated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human
hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching
at different temperatures and data obtained from the van’t Hoff diagram and molecular docking
indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site
for all drugs in the central cavity near αβ interfaces and is dominantly mediated through hydrophobic
forces. The association constants were lower-moderate strength (~104 M−1
), the highest observed
for clozapine (2.2 × 104 M−1 at 25 ◦C). The clozapine binding showed “friendly” effects: increased
α-helical content, a higher melting point, and protein protection from free radical-mediated oxidation.
On the other hand, bound ziprasidone and sertindole had a slightly pro-oxidative effect, increasing
ferrihemoglobin content, a possible “foe”. Since the interaction of proteins with drugs plays a vital
role in their pharmacokinetic and pharmacodynamic properties, the physiological significance of the
obtained findings is briefly discussed.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?",
number = "10",
volume = "24",
doi = "10.3390/ijms24108921",
pages = "8921"
}

Platanić-Arizanović, L., Gligorijević, N., Cvijetić, I., Mijatović, A., Krstić-Ristivojević, M., Minić, S., Nikolić-Kokić, A., Miljević, Č.,& Nikolić, M.. (2023). Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?. in International Journal of Molecular Sciences
Basel: MDPI., 24(10), 8921.
https://doi.org/10.3390/ijms24108921

Platanić-Arizanović L, Gligorijević N, Cvijetić I, Mijatović A, Krstić-Ristivojević M, Minić S, Nikolić-Kokić A, Miljević Č, Nikolić M. Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?. in International Journal of Molecular Sciences. 2023;24(10):8921.
doi:10.3390/ijms24108921 .

Platanić-Arizanović, Lena, Gligorijević, Nikola, Cvijetić, Ilija, Mijatović, Aleksandar, Krstić-Ristivojević, Maja, Minić, Simeon, Nikolić-Kokić, Aleksandra, Miljević, Čedo, Nikolić, Milan, "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?" in International Journal of Molecular Sciences, 24, no. 10 (2023):8921,
https://doi.org/10.3390/ijms24108921 . .

TY  - JOUR
AU  - Stojković, Pavle
AU  - Stepanović, Ana
AU  - Lupšić, Ema
AU  - Terzić Jovanović, Nataša
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5910
AB  - The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo
[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic
properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine
linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f
consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and
11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out
of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 μM. The
concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding
multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and
normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven
compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All
compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a,
12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased
P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its
precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial
membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma
cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress
accompanied by inhibition of mitochondria.
PB  - Academic Press Inc.
T2  - Bioorganic Chemistry
T1  - Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells
VL  - 138
DO  - 10.1016/j.bioorg.2023.106605
SP  - 106605
ER  - 

@article{
author = "Stojković, Pavle and Stepanović, Ana and Lupšić, Ema and Terzić Jovanović, Nataša and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Pešić, Milica and Opsenica, Igor M.",
year = "2023",
abstract = "The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo
[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic
properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine
linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f
consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and
11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out
of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 μM. The
concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding
multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and
normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven
compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All
compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a,
12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased
P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its
precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial
membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma
cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress
accompanied by inhibition of mitochondria.",
publisher = "Academic Press Inc.",
journal = "Bioorganic Chemistry",
title = "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells",
volume = "138",
doi = "10.1016/j.bioorg.2023.106605",
pages = "106605"
}

Stojković, P., Stepanović, A., Lupšić, E., Terzić Jovanović, N., Novaković, M., Nedialkov, P., Trendafilova, A., Pešić, M.,& Opsenica, I. M.. (2023). Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry
Academic Press Inc.., 138, 106605.
https://doi.org/10.1016/j.bioorg.2023.106605

Stojković P, Stepanović A, Lupšić E, Terzić Jovanović N, Novaković M, Nedialkov P, Trendafilova A, Pešić M, Opsenica IM. Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry. 2023;138:106605.
doi:10.1016/j.bioorg.2023.106605 .

Stojković, Pavle, Stepanović, Ana, Lupšić, Ema, Terzić Jovanović, Nataša, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Pešić, Milica, Opsenica, Igor M., "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells" in Bioorganic Chemistry, 138 (2023):106605,
https://doi.org/10.1016/j.bioorg.2023.106605 . .

TY  - JOUR
AU  - Koračak, Ljiljana
AU  - Lupšić, Ema
AU  - Terzić Jovanović, Nataša
AU  - Jovanović, Mirna
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Zlatović, Mario
AU  - Pešić, Milica
AU  - Opsenica, Igor
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5890
AB  - The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.
PB  - Cambridge: Royal Society of Chemistry
T2  - New Journal of Chemistry
T1  - Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells
IS  - 14
VL  - 47
DO  - 10.1039/D3NJ00427A
SP  - 6844
EP  - 6855
ER  - 

@article{
author = "Koračak, Ljiljana and Lupšić, Ema and Terzić Jovanović, Nataša and Jovanović, Mirna and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Zlatović, Mario and Pešić, Milica and Opsenica, Igor",
year = "2023",
abstract = "The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "New Journal of Chemistry",
title = "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells",
number = "14",
volume = "47",
doi = "10.1039/D3NJ00427A",
pages = "6844-6855"
}

Koračak, L., Lupšić, E., Terzić Jovanović, N., Jovanović, M., Novaković, M., Nedialkov, P., Trendafilova, A., Zlatović, M., Pešić, M.,& Opsenica, I.. (2023). Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry
Cambridge: Royal Society of Chemistry., 47(14), 6844-6855.
https://doi.org/10.1039/D3NJ00427A

Koračak L, Lupšić E, Terzić Jovanović N, Jovanović M, Novaković M, Nedialkov P, Trendafilova A, Zlatović M, Pešić M, Opsenica I. Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry. 2023;47(14):6844-6855.
doi:10.1039/D3NJ00427A .

Koračak, Ljiljana, Lupšić, Ema, Terzić Jovanović, Nataša, Jovanović, Mirna, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Zlatović, Mario, Pešić, Milica, Opsenica, Igor, "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells" in New Journal of Chemistry, 47, no. 14 (2023):6844-6855,
https://doi.org/10.1039/D3NJ00427A . .

TY  - JOUR
AU  - Pantović Pavlović, Marijana R.
AU  - Ignjatović, Nenad L.
AU  - Panić, Vladimir V.
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Malešević, Anastasija
AU  - Pavlović, Miroslav M.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5554
AB  - The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous
calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF- ) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1  and TNF, lower expression of TGF-  in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.
PB  - Basel: MDPI
T2  - Journal of Functional Biomaterials
T1  - Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants
IS  - 4
VL  - 14
DO  - 10.3390/jfb14040227
SP  - 227
ER  - 

@article{
author = "Pantović Pavlović, Marijana R. and Ignjatović, Nenad L. and Panić, Vladimir V. and Mirkov, Ivana and Kulaš, Jelena and Malešević, Anastasija and Pavlović, Miroslav M.",
year = "2023",
abstract = "The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous
calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF- ) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1  and TNF, lower expression of TGF-  in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.",
publisher = "Basel: MDPI",
journal = "Journal of Functional Biomaterials",
title = "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants",
number = "4",
volume = "14",
doi = "10.3390/jfb14040227",
pages = "227"
}

Pantović Pavlović, M. R., Ignjatović, N. L., Panić, V. V., Mirkov, I., Kulaš, J., Malešević, A.,& Pavlović, M. M.. (2023). Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials
Basel: MDPI., 14(4), 227.
https://doi.org/10.3390/jfb14040227

Pantović Pavlović MR, Ignjatović NL, Panić VV, Mirkov I, Kulaš J, Malešević A, Pavlović MM. Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials. 2023;14(4):227.
doi:10.3390/jfb14040227 .

Pantović Pavlović, Marijana R., Ignjatović, Nenad L., Panić, Vladimir V., Mirkov, Ivana, Kulaš, Jelena, Malešević, Anastasija, Pavlović, Miroslav M., "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants" in Journal of Functional Biomaterials, 14, no. 4 (2023):227,
https://doi.org/10.3390/jfb14040227 . .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6594
AB  - In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.
PB  - Skopje: Macedonian Pharmaceutical Association
C3  - 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
T1  - Anti-melanoma effects of ingenanes isolated from Euphorbia species
DO  - 10.33320/maced.pharm.bull.2022.68.04.006
SP  - 23
EP  - 24
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Jovanović Stojanov, Sofija and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.",
publisher = "Skopje: Macedonian Pharmaceutical Association",
journal = "11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia",
title = "Anti-melanoma effects of ingenanes isolated from Euphorbia species",
doi = "10.33320/maced.pharm.bull.2022.68.04.006",
pages = "23-24"
}

Krstić, G., Jadranin, M., Jovanović Stojanov, S., Pešić, M., Tešević, V.,& Milosavljević, S.. (2022). Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
Skopje: Macedonian Pharmaceutical Association., 23-24.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006

Krstić G, Jadranin M, Jovanović Stojanov S, Pešić M, Tešević V, Milosavljević S. Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia. 2022;:23-24.
doi:10.33320/maced.pharm.bull.2022.68.04.006 .

Krstić, Gordana, Jadranin, Milka, Jovanović Stojanov, Sofija, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, "Anti-melanoma effects of ingenanes isolated from Euphorbia species" in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia (2022):23-24,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006 . .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6588
AB  - Rak je vodeći uzrok smrti ljudi širom sveta sa 10 miliona umrlih u 2020. godini
zbog čega je jedan od imperativa naučne zajednice pronalazak efikasnijih
hemioterapeutika. Više od polovine lekova prirodnog je porekla, a budući da
okeani čine oko 70% Zemljine površine, marinski ekosistem je odlična osnova za
dobijanje novih lekova. Hinoni avarol i avaron su redoks par izolovan iz
mediteranskog sunđera Disidea avara i pokazuju raznovrsnu biološku aktivnost
poput antimikrobne i antitumorske. Кao i kod većine marinskih organizama,
najveće probleme za njihovu primenu predstavljaju slab prinos i cena
eksploatacije. Rešenje problema može biti sinteza jedinjenja sličnih po
hemijskoj strukturi i delovanju avarolu/avaronu poput terc-butilhinona (TBQ),
a sintezom alkiltio i ariltio derivata dodatno povećanje njegove aktivnosti.
Uporedno je testiran antibakterijski, toksični, citotoksični, genotoksični,
antioksidativni i antineurodegenerativni potencijal TBQ i njegovih derivata.
Generalno, derivati TBQ pokazuju jaču biološku aktivnost. Najjača
antibakterijska aktivnost uočena je na S. aureus i B. subtilis. Modifikacije
povećavaju citotoksičnost i genotoksičnost na humanim ćelijskim linijama dok
derivat 2-terc-butil-5,6-(etileneditio)-1,4-benzohinon pokazuje najjaču
aktivnost i na osnovu γH2AX testa dovodi do dvolančanih prekida DNК
molekula. Sva ispitivana jedinjenja snažno zaustavljaju ćelijski ciklus u G0/G1
fazi. Dodatno, jedinjenja pokazuju dobar antioksidativni i anttineurodegenerativni potencijal.
AB  - Рак је водећи узрок смрти људи широм света са 10 милиона умрлих у 2020. години
због чега је један од императива научне заједнице проналазак ефикаснијих
хемиотерапеутика. Више од половине лекова природног је порекла, а будући да
океани чине око 70% Земљине површине, марински екосистем је одлична основа за
добијање нових лекова. Хинони аварол и аварон су редокс пар изолован из
медитеранског сунђера Disidea avara и показују разноврсну биолошку активност
попут антимикробне и антитуморске. Као и код већине маринских организама,
највећe проблемe за њихову примену представљају слаб принос и цена
експлоатације. Решење проблема може бити синтеза једињења сличних по
хемијској структури и деловању аваролу/аварону попут терц-бутилхинона (ТBQ),
а синтезом алкилтио и арилтио деривата додатно повећање његове активности.
Упоредно је тестиран антибактеријски, токсични, цитотоксични, генотоксични,
антиоксидативни и антинеуродегенеративни потенцијал ТBQ и његових деривата.
Генерално, деривати ТBQ показују јачу биолошку активност. Најјача
антибактеријска активност уочена је на S. aureus и B. subtilis. Модификације
повећавају цитотоксичност и генотоксичност на хуманим ћелијским линијама док
дериват 2-терц-бутил-5,6-(етиленедитио)-1,4-бензохинон показује најјачу
активност и на основу γH2AX теста доводи до дволанчаних прекида ДНК
молекула. Сва испитивана једињења снажно заустављају ћелијски циклус у Г0/Г1
фази. Додатно, једињења показују добар антиоксидативни и
анттинеуродегенеративни потенцијал.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata.
T1  - Упоредни приказ биолошкe активности потенцијалних антитуморских агенаса: терц-бутилхинона и његових алкилтио и арилтио деривата
SP  - 251
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6588
ER  - 

@conference{
author = "Đorđević, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Rak je vodeći uzrok smrti ljudi širom sveta sa 10 miliona umrlih u 2020. godini
zbog čega je jedan od imperativa naučne zajednice pronalazak efikasnijih
hemioterapeutika. Više od polovine lekova prirodnog je porekla, a budući da
okeani čine oko 70% Zemljine površine, marinski ekosistem je odlična osnova za
dobijanje novih lekova. Hinoni avarol i avaron su redoks par izolovan iz
mediteranskog sunđera Disidea avara i pokazuju raznovrsnu biološku aktivnost
poput antimikrobne i antitumorske. Кao i kod većine marinskih organizama,
najveće probleme za njihovu primenu predstavljaju slab prinos i cena
eksploatacije. Rešenje problema može biti sinteza jedinjenja sličnih po
hemijskoj strukturi i delovanju avarolu/avaronu poput terc-butilhinona (TBQ),
a sintezom alkiltio i ariltio derivata dodatno povećanje njegove aktivnosti.
Uporedno je testiran antibakterijski, toksični, citotoksični, genotoksični,
antioksidativni i antineurodegenerativni potencijal TBQ i njegovih derivata.
Generalno, derivati TBQ pokazuju jaču biološku aktivnost. Najjača
antibakterijska aktivnost uočena je na S. aureus i B. subtilis. Modifikacije
povećavaju citotoksičnost i genotoksičnost na humanim ćelijskim linijama dok
derivat 2-terc-butil-5,6-(etileneditio)-1,4-benzohinon pokazuje najjaču
aktivnost i na osnovu γH2AX testa dovodi do dvolančanih prekida DNК
molekula. Sva ispitivana jedinjenja snažno zaustavljaju ćelijski ciklus u G0/G1
fazi. Dodatno, jedinjenja pokazuju dobar antioksidativni i anttineurodegenerativni potencijal., Рак је водећи узрок смрти људи широм света са 10 милиона умрлих у 2020. години
због чега је један од императива научне заједнице проналазак ефикаснијих
хемиотерапеутика. Више од половине лекова природног је порекла, а будући да
океани чине око 70% Земљине површине, марински екосистем је одлична основа за
добијање нових лекова. Хинони аварол и аварон су редокс пар изолован из
медитеранског сунђера Disidea avara и показују разноврсну биолошку активност
попут антимикробне и антитуморске. Као и код већине маринских организама,
највећe проблемe за њихову примену представљају слаб принос и цена
експлоатације. Решење проблема може бити синтеза једињења сличних по
хемијској структури и деловању аваролу/аварону попут терц-бутилхинона (ТBQ),
а синтезом алкилтио и арилтио деривата додатно повећање његове активности.
Упоредно је тестиран антибактеријски, токсични, цитотоксични, генотоксични,
антиоксидативни и антинеуродегенеративни потенцијал ТBQ и његових деривата.
Генерално, деривати ТBQ показују јачу биолошку активност. Најјача
антибактеријска активност уочена је на S. aureus и B. subtilis. Модификације
повећавају цитотоксичност и генотоксичност на хуманим ћелијским линијама док
дериват 2-терц-бутил-5,6-(етиленедитио)-1,4-бензохинон показује најјачу
активност и на основу γH2AX теста доводи до дволанчаних прекида ДНК
молекула. Сва испитивана једињења снажно заустављају ћелијски циклус у Г0/Г1
фази. Додатно, једињења показују добар антиоксидативни и
анттинеуродегенеративни потенцијал.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata., Упоредни приказ биолошкe активности потенцијалних антитуморских агенаса: терц-бутилхинона и његових алкилтио и арилтио деривата",
pages = "251",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6588"
}

Đorđević, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 251.
https://hdl.handle.net/21.15107/rcub_ibiss_6588

Đorđević J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:251.
https://hdl.handle.net/21.15107/rcub_ibiss_6588 .

Đorđević, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata." in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):251,
https://hdl.handle.net/21.15107/rcub_ibiss_6588 .

TY  - JOUR
AU  - Miletić, Srđan
AU  - Nikolić-Kokić, Aleksandra
AU  - Jovanović, Dara
AU  - Žerađanin, Aleksandra
AU  - Joksimović, Kristina
AU  - Avdalović, Jelena
AU  - Spasić, Snežana
PY  - 2022
UR  - https://link.springer.com/10.1134/S1068162023010193
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5360
AB  - In this research, the antioxidant activity of acidic and alkaline hydrolyzates of pectin isolated from quince (Cydonia oblonga) was investigated. The antioxidant role of quince has been known since before, but so far few papers have been published dealing with the antioxidant role of pectin hydrolyzate. In this study, quince pectin was isolated and hydrolyzed by acid and alkaline hydrolysis. Antioxidant activity was examined using a number of different methods, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assay, iron chelators, and oxygen radical absorption capacity (ORAC). Our results, especially the ORAC test, show that depolymerization produces the most substances with antioxidant release properties in the alkaline hydrolysis of quince pectin.
PB  - Pleiades Publishing
T2  - Russian Journal of Bioorganic Chemistry
T1  - Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga)
DO  - 10.1134/S1068162023010193
ER  - 

@article{
author = "Miletić, Srđan and Nikolić-Kokić, Aleksandra and Jovanović, Dara and Žerađanin, Aleksandra and Joksimović, Kristina and Avdalović, Jelena and Spasić, Snežana",
year = "2022",
abstract = "In this research, the antioxidant activity of acidic and alkaline hydrolyzates of pectin isolated from quince (Cydonia oblonga) was investigated. The antioxidant role of quince has been known since before, but so far few papers have been published dealing with the antioxidant role of pectin hydrolyzate. In this study, quince pectin was isolated and hydrolyzed by acid and alkaline hydrolysis. Antioxidant activity was examined using a number of different methods, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assay, iron chelators, and oxygen radical absorption capacity (ORAC). Our results, especially the ORAC test, show that depolymerization produces the most substances with antioxidant release properties in the alkaline hydrolysis of quince pectin.",
publisher = "Pleiades Publishing",
journal = "Russian Journal of Bioorganic Chemistry",
title = "Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga)",
doi = "10.1134/S1068162023010193"
}

Miletić, S., Nikolić-Kokić, A., Jovanović, D., Žerađanin, A., Joksimović, K., Avdalović, J.,& Spasić, S.. (2022). Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga). in Russian Journal of Bioorganic Chemistry
Pleiades Publishing..
https://doi.org/10.1134/S1068162023010193

Miletić S, Nikolić-Kokić A, Jovanović D, Žerađanin A, Joksimović K, Avdalović J, Spasić S. Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga). in Russian Journal of Bioorganic Chemistry. 2022;.
doi:10.1134/S1068162023010193 .

Miletić, Srđan, Nikolić-Kokić, Aleksandra, Jovanović, Dara, Žerađanin, Aleksandra, Joksimović, Kristina, Avdalović, Jelena, Spasić, Snežana, "Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga)" in Russian Journal of Bioorganic Chemistry (2022),
https://doi.org/10.1134/S1068162023010193 . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav
AU  - Vovk, Irena
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941463/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9263698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5080
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.
VL  - 9
DO  - 10.3389/fnut.2022.941463
SP  - 941463
ER  - 

@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.",
volume = "9",
doi = "10.3389/fnut.2022.941463",
pages = "941463"
}

Nedić, N., Nešović, M., Radišić, P., Gašić, U., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463

Nedić N, Nešović M, Radišić P, Gašić U, Baošić R, Joksimović K, Pezo L, Tešić Ž, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .

Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš, Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav, Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia." in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .

TY  - CONF
AU  - Dragoj, Miodrag
AU  - Dinić, Jelena
AU  - Podolski-Renić, Ana
AU  - Jovanović Stojanov, Sofija
AU  - Jovanović, Mirna
AU  - Stepanović, Ana
AU  - Ercegovac, Maja
AU  - Marić, Dragana
AU  - Pešić, Milica
PY  - 2022
UR  - https://stratagem-cost.eu/2022/04/stratagems-5th-co-located-annual-conference-and-wg3-4-training-school-to-be-held-in-coimbra-portugal-june-july-2022/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5371
AB  - Although advances in sequencing technology and target identification enabled the implementation of a
personalized therapy approach, unfortunately, only 3-9% of cancer patients who receive the targeted
therapy show an adequate response. On the other side, there are exceptional responders to targeted
therapy among cancer patients without common genetic alterations. Therefore, current patient
classifications relying only on sequencing are not sufficient to determine optimal treatment. Our
intention is to start in the opposite direction to conventional diagnostics by performing pharmacological
screening on patient-derived cancer cells ex vivo because testing of multiple drugs is not possible in
clinical trials. An incomplete understanding of how tumour genotype reflects on tumour phenotype
limits the efficacy of DNA and mRNA sequencing for personalized therapy. Functional diagnostics using
patient-derived cancer cells is recently implicated to overcome this limitation and it is clinically available
for haematological malignancies. We plan to perform the immunofluorescence-based drug-screening
assay to determine non-small cell lung carcinoma (NSCLC) patients’ cancer cells’ response to targeted
therapeutics, particularly tyrosine kinase inhibitors (TKIs) within the time frame necessary to influence
patient care. The usage of the functional diagnostics approach should be an addition to clinical trials and
complement DNA and mRNA sequencing.
In contrast to similar research efforts [1], we will shorten the cultivation of NSCLC patient-derived
cells to 1-2 weeks because we intend to test drugs on a mixture of cancer and stromal cells (fibroblasts).
It is well-known that the sensitivity of cancer cells depends on their interaction with the
microenvironment including neighbouring cells. In addition, we will examine the changes in the
expression level of ATP Binding Cassette transporters (ABCB1, ABCC1, and ABCG2) in both cancer
and stromal cells that may occur during TKIs and chemotherapy treatment. In such way, we will gain
knowledge about (i) which TKI or chemotherapeutic induces multidrug-resistant (MDR) phenotype in
our NSCLC patients’ cohort, (ii) whether the induction of MDR depends on the ratio between cancer
and stromal cells, (iii) whether the induction of MDR is prevalent in cancer cells, and (iv) whether MDR
induction depends on individual patient’s characteristics (comparison with Whole Exome Sequencing
results).
PB  - STRATAGEM COST Action
C3  - Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal
T1  - Functional diagnostics as a new concept for the improvement of personalized targeted therapy
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5371
ER  - 

@conference{
author = "Dragoj, Miodrag and Dinić, Jelena and Podolski-Renić, Ana and Jovanović Stojanov, Sofija and Jovanović, Mirna and Stepanović, Ana and Ercegovac, Maja and Marić, Dragana and Pešić, Milica",
year = "2022",
abstract = "Although advances in sequencing technology and target identification enabled the implementation of a
personalized therapy approach, unfortunately, only 3-9% of cancer patients who receive the targeted
therapy show an adequate response. On the other side, there are exceptional responders to targeted
therapy among cancer patients without common genetic alterations. Therefore, current patient
classifications relying only on sequencing are not sufficient to determine optimal treatment. Our
intention is to start in the opposite direction to conventional diagnostics by performing pharmacological
screening on patient-derived cancer cells ex vivo because testing of multiple drugs is not possible in
clinical trials. An incomplete understanding of how tumour genotype reflects on tumour phenotype
limits the efficacy of DNA and mRNA sequencing for personalized therapy. Functional diagnostics using
patient-derived cancer cells is recently implicated to overcome this limitation and it is clinically available
for haematological malignancies. We plan to perform the immunofluorescence-based drug-screening
assay to determine non-small cell lung carcinoma (NSCLC) patients’ cancer cells’ response to targeted
therapeutics, particularly tyrosine kinase inhibitors (TKIs) within the time frame necessary to influence
patient care. The usage of the functional diagnostics approach should be an addition to clinical trials and
complement DNA and mRNA sequencing.
In contrast to similar research efforts [1], we will shorten the cultivation of NSCLC patient-derived
cells to 1-2 weeks because we intend to test drugs on a mixture of cancer and stromal cells (fibroblasts).
It is well-known that the sensitivity of cancer cells depends on their interaction with the
microenvironment including neighbouring cells. In addition, we will examine the changes in the
expression level of ATP Binding Cassette transporters (ABCB1, ABCC1, and ABCG2) in both cancer
and stromal cells that may occur during TKIs and chemotherapy treatment. In such way, we will gain
knowledge about (i) which TKI or chemotherapeutic induces multidrug-resistant (MDR) phenotype in
our NSCLC patients’ cohort, (ii) whether the induction of MDR depends on the ratio between cancer
and stromal cells, (iii) whether the induction of MDR is prevalent in cancer cells, and (iv) whether MDR
induction depends on individual patient’s characteristics (comparison with Whole Exome Sequencing
results).",
publisher = "STRATAGEM COST Action",
journal = "Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal",
title = "Functional diagnostics as a new concept for the improvement of personalized targeted therapy",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5371"
}

Dragoj, M., Dinić, J., Podolski-Renić, A., Jovanović Stojanov, S., Jovanović, M., Stepanović, A., Ercegovac, M., Marić, D.,& Pešić, M.. (2022). Functional diagnostics as a new concept for the improvement of personalized targeted therapy. in Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal
STRATAGEM COST Action., 94.
https://hdl.handle.net/21.15107/rcub_ibiss_5371

Dragoj M, Dinić J, Podolski-Renić A, Jovanović Stojanov S, Jovanović M, Stepanović A, Ercegovac M, Marić D, Pešić M. Functional diagnostics as a new concept for the improvement of personalized targeted therapy. in Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal. 2022;:94.
https://hdl.handle.net/21.15107/rcub_ibiss_5371 .

Dragoj, Miodrag, Dinić, Jelena, Podolski-Renić, Ana, Jovanović Stojanov, Sofija, Jovanović, Mirna, Stepanović, Ana, Ercegovac, Maja, Marić, Dragana, Pešić, Milica, "Functional diagnostics as a new concept for the improvement of personalized targeted therapy" in Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal (2022):94,
https://hdl.handle.net/21.15107/rcub_ibiss_5371 .

TY  - CONF
AU  - Stojković, Pavle
AU  - Stepanović, Ana
AU  - Terzić Jovanović, Nataša
AU  - Novaković, Miroslav
AU  - Trendafilova, Antoaneta
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2022
UR  - https://euchems2022.eu/images/abstracts.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5372
AB  - Sclareol is a labdane diterpenoid found in clary sage (Salvia sclarea L.) with various biological activities,
most notably anticancer and cytotoxic activity [1]. There are several examples of synthetic derivatives
of sclareol with antischistosomal [2], antifungal [3], and anticancer activity [4]. Since it is known that
modifications of biologically active molecules can lead to the improvement of physicochemical
properties and modes of interactions with target cells, we have envisioned the derivatization of sclareol
to obtain molecules with more potent cytotoxic activity.
Sclareol used as a starting material in this research was isolated from Clary sage harvested in Bulgaria.
New compounds were obtained by derivatization of sclareol at its Δ14,15 double bond using oxidative
Heck coupling catalyzed by palladium-acetate with copper(II)-acetate as oxidant, followed by the
introduction of different diamine-moieties. Finally, the terminal amino-group was coupled with a
nitrogen-rich heterocycle to obtain desired compounds. During the course of the synthesis, both tertiary
and tertiary allylic hydroxyl groups remained unchanged, which was of particular interest, since it was
shown that the tertiary allylic group is crucial for the biological activity of sclareol.
Synthesized compounds were tested on human cancer cell lines, primarily glioblastoma cells. It was
shown that certain derivatives have caused a significant reduction of glioblastoma cell viability at low
concentrations. Moreover, some derivatives inhibited cell membrane transporter P-glycoprotein (P-gp)
responsible for multidrug resistance and increased accumulation of doxorubicin to the same extent as
tariquidar (a well-known P-gp inhibitor). Most importantly, novel molecules exhibited more potent
biological activity than sclareol itself.
PB  - Sociedade Portuguesa de Química
C3  - Abstract Book: 8th EuChemS Chemistry Congress; 2022 Aug 28 - Sep 1; Lisbon, Portugal
T1  - Synthesis of novel sclareol derivatives and evaluation of their anticancer activity
SP  - 604
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5372
ER  - 

@conference{
author = "Stojković, Pavle and Stepanović, Ana and Terzić Jovanović, Nataša and Novaković, Miroslav and Trendafilova, Antoaneta and Pešić, Milica and Opsenica, Igor M.",
year = "2022",
abstract = "Sclareol is a labdane diterpenoid found in clary sage (Salvia sclarea L.) with various biological activities,
most notably anticancer and cytotoxic activity [1]. There are several examples of synthetic derivatives
of sclareol with antischistosomal [2], antifungal [3], and anticancer activity [4]. Since it is known that
modifications of biologically active molecules can lead to the improvement of physicochemical
properties and modes of interactions with target cells, we have envisioned the derivatization of sclareol
to obtain molecules with more potent cytotoxic activity.
Sclareol used as a starting material in this research was isolated from Clary sage harvested in Bulgaria.
New compounds were obtained by derivatization of sclareol at its Δ14,15 double bond using oxidative
Heck coupling catalyzed by palladium-acetate with copper(II)-acetate as oxidant, followed by the
introduction of different diamine-moieties. Finally, the terminal amino-group was coupled with a
nitrogen-rich heterocycle to obtain desired compounds. During the course of the synthesis, both tertiary
and tertiary allylic hydroxyl groups remained unchanged, which was of particular interest, since it was
shown that the tertiary allylic group is crucial for the biological activity of sclareol.
Synthesized compounds were tested on human cancer cell lines, primarily glioblastoma cells. It was
shown that certain derivatives have caused a significant reduction of glioblastoma cell viability at low
concentrations. Moreover, some derivatives inhibited cell membrane transporter P-glycoprotein (P-gp)
responsible for multidrug resistance and increased accumulation of doxorubicin to the same extent as
tariquidar (a well-known P-gp inhibitor). Most importantly, novel molecules exhibited more potent
biological activity than sclareol itself.",
publisher = "Sociedade Portuguesa de Química",
journal = "Abstract Book: 8th EuChemS Chemistry Congress; 2022 Aug 28 - Sep 1; Lisbon, Portugal",
title = "Synthesis of novel sclareol derivatives and evaluation of their anticancer activity",
pages = "604",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5372"
}

Stojković, P., Stepanović, A., Terzić Jovanović, N., Novaković, M., Trendafilova, A., Pešić, M.,& Opsenica, I. M.. (2022). Synthesis of novel sclareol derivatives and evaluation of their anticancer activity. in Abstract Book: 8th EuChemS Chemistry Congress; 2022 Aug 28 - Sep 1; Lisbon, Portugal
Sociedade Portuguesa de Química., 604.
https://hdl.handle.net/21.15107/rcub_ibiss_5372

Stojković P, Stepanović A, Terzić Jovanović N, Novaković M, Trendafilova A, Pešić M, Opsenica IM. Synthesis of novel sclareol derivatives and evaluation of their anticancer activity. in Abstract Book: 8th EuChemS Chemistry Congress; 2022 Aug 28 - Sep 1; Lisbon, Portugal. 2022;:604.
https://hdl.handle.net/21.15107/rcub_ibiss_5372 .

Stojković, Pavle, Stepanović, Ana, Terzić Jovanović, Nataša, Novaković, Miroslav, Trendafilova, Antoaneta, Pešić, Milica, Opsenica, Igor M., "Synthesis of novel sclareol derivatives and evaluation of their anticancer activity" in Abstract Book: 8th EuChemS Chemistry Congress; 2022 Aug 28 - Sep 1; Lisbon, Portugal (2022):604,
https://hdl.handle.net/21.15107/rcub_ibiss_5372 .

TY  - CONF
AU  - Stepanović, Ana
AU  - Stojković, Pavle
AU  - Terzić Jovanović, Nataša
AU  - Novaković, Miroslav
AU  - Opsenica, Igor M.
AU  - Pešić, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5370
AB  - Background: Natural products exhibit a wide range of biological activities and they are starting point in the drug discovery process. Sclareol (SCL) naturally occurring labdane diterpene isolated from Clary sage (Salvia sclarea L.) shows diverse biological properties such as antioxidative, antimicrobial, anti-inflammatory, and anticancer activities. It is well established that fusing two pharmacophores can lead to significant improvement in the biological potential of the molecule by modifying its physicochemical properties. We envisioned that chimeric molecules synthesized by linking triazolo[1,5-a]pyrimidine pharmacophore to SCL would have more potent anticancer activity than their parental compound – SCL. Therefore, the cytotoxic potential of a series of SCL derivatives was compared with SCL. We have also studied their potential to increase the accumulation of substrates of membrane transporter which causes resistance of cancer cells – P-glycoprotein (P-gp). Methods: Cytotoxic potential, selectivity towards cancer cells, and resistance profile of SCL and its derivatives were examined by MTT assay after 72 h exposure in human glioblastoma cells (sensitive U87 and multidrug-resistant U87-TxR) and rat microglial cells (BV-2). We also investigated the effect of SCL and its derivatives on P-gp activity in U87-TxR resistant cells by determining the level of accumulated P-gp substrates (rhodamine 123 and doxorubicin) by flow cytometry. Results: More than half of the tested SCL derivatives considerably reduced glioblastoma cell viability with a concentration of 5 μM. Tested compounds evaded the resistance of glioblastoma cells showing similar or better activity against U87-TxR cells in comparison with U87 cells. All compounds significantly increased the accumulation of rhodamine 123 pointing to the inhibition of P-gp. However, only three of them increased the accumulation of doxorubicin likewise tariquidar, a well-known third-generation P-gp inhibitor, implying that these three SCL derivatives can be valuable as chemo-sensitizing agents. Conclusion: Our results showed that SCL derivatives can be considered as modulators of P-gp activity especially pointing to several lead compounds whose detailed molecular mechanism of anticancer action should be studied.
PB  - STRATAGEM COST Action
C3  - Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal
T1  - Hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine inhibit P-glycoprotein function in glioblastoma cells
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5370
ER  - 

@conference{
author = "Stepanović, Ana and Stojković, Pavle and Terzić Jovanović, Nataša and Novaković, Miroslav and Opsenica, Igor M. and Pešić, Milica",
year = "2022",
abstract = "Background: Natural products exhibit a wide range of biological activities and they are starting point in the drug discovery process. Sclareol (SCL) naturally occurring labdane diterpene isolated from Clary sage (Salvia sclarea L.) shows diverse biological properties such as antioxidative, antimicrobial, anti-inflammatory, and anticancer activities. It is well established that fusing two pharmacophores can lead to significant improvement in the biological potential of the molecule by modifying its physicochemical properties. We envisioned that chimeric molecules synthesized by linking triazolo[1,5-a]pyrimidine pharmacophore to SCL would have more potent anticancer activity than their parental compound – SCL. Therefore, the cytotoxic potential of a series of SCL derivatives was compared with SCL. We have also studied their potential to increase the accumulation of substrates of membrane transporter which causes resistance of cancer cells – P-glycoprotein (P-gp). Methods: Cytotoxic potential, selectivity towards cancer cells, and resistance profile of SCL and its derivatives were examined by MTT assay after 72 h exposure in human glioblastoma cells (sensitive U87 and multidrug-resistant U87-TxR) and rat microglial cells (BV-2). We also investigated the effect of SCL and its derivatives on P-gp activity in U87-TxR resistant cells by determining the level of accumulated P-gp substrates (rhodamine 123 and doxorubicin) by flow cytometry. Results: More than half of the tested SCL derivatives considerably reduced glioblastoma cell viability with a concentration of 5 μM. Tested compounds evaded the resistance of glioblastoma cells showing similar or better activity against U87-TxR cells in comparison with U87 cells. All compounds significantly increased the accumulation of rhodamine 123 pointing to the inhibition of P-gp. However, only three of them increased the accumulation of doxorubicin likewise tariquidar, a well-known third-generation P-gp inhibitor, implying that these three SCL derivatives can be valuable as chemo-sensitizing agents. Conclusion: Our results showed that SCL derivatives can be considered as modulators of P-gp activity especially pointing to several lead compounds whose detailed molecular mechanism of anticancer action should be studied.",
publisher = "STRATAGEM COST Action",
journal = "Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal",
title = "Hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine inhibit P-glycoprotein function in glioblastoma cells",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5370"
}

Stepanović, A., Stojković, P., Terzić Jovanović, N., Novaković, M., Opsenica, I. M.,& Pešić, M.. (2022). Hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine inhibit P-glycoprotein function in glioblastoma cells. in Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal
STRATAGEM COST Action., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5370

Stepanović A, Stojković P, Terzić Jovanović N, Novaković M, Opsenica IM, Pešić M. Hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine inhibit P-glycoprotein function in glioblastoma cells. in Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5370 .

Stepanović, Ana, Stojković, Pavle, Terzić Jovanović, Nataša, Novaković, Miroslav, Opsenica, Igor M., Pešić, Milica, "Hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine inhibit P-glycoprotein function in glioblastoma cells" in Abstract Book: STRATAGEM’s 5th Annual Meeting: New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumours; 2022 Jun 29 - Jul 1; Coimbra, Portugal (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5370 .

TY  - JOUR
AU  - Krunić, Matija
AU  - Ristić, Biljana
AU  - Bošnjak, Mihajlo
AU  - Paunović, Verica
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Mirčić, Aleksandar
AU  - Marković, Zoran
AU  - Todorović-Marković, Biljana
AU  - Jovanović, Svetlana
AU  - Kleut, Duška
AU  - Mojović, Miloš
AU  - Nakarada, Đura
AU  - Marković, Olivera
AU  - Vuković, Irena
AU  - Harhaji-Trajković, Ljubica
AU  - Trajković, Vladimir
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0891584921007760
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4655
AB  - We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•-), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.
PB  - Elsevier Inc.
T2  - Free Radical Biology and Medicine
T1  - Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.10.025
SP  - 167
EP  - 180
ER  - 

@article{
author = "Krunić, Matija and Ristić, Biljana and Bošnjak, Mihajlo and Paunović, Verica and Tovilović-Kovačević, Gordana and Zogović, Nevena and Mirčić, Aleksandar and Marković, Zoran and Todorović-Marković, Biljana and Jovanović, Svetlana and Kleut, Duška and Mojović, Miloš and Nakarada, Đura and Marković, Olivera and Vuković, Irena and Harhaji-Trajković, Ljubica and Trajković, Vladimir",
year = "2021",
abstract = "We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•-), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.",
publisher = "Elsevier Inc.",
journal = "Free Radical Biology and Medicine",
title = "Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.10.025",
pages = "167-180"
}

Krunić, M., Ristić, B., Bošnjak, M., Paunović, V., Tovilović-Kovačević, G., Zogović, N., Mirčić, A., Marković, Z., Todorović-Marković, B., Jovanović, S., Kleut, D., Mojović, M., Nakarada, Đ., Marković, O., Vuković, I., Harhaji-Trajković, L.,& Trajković, V.. (2021). Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.. in Free Radical Biology and Medicine
Elsevier Inc.., 177, 167-180.
https://doi.org/10.1016/j.freeradbiomed.2021.10.025

Krunić M, Ristić B, Bošnjak M, Paunović V, Tovilović-Kovačević G, Zogović N, Mirčić A, Marković Z, Todorović-Marković B, Jovanović S, Kleut D, Mojović M, Nakarada Đ, Marković O, Vuković I, Harhaji-Trajković L, Trajković V. Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.. in Free Radical Biology and Medicine. 2021;177:167-180.
doi:10.1016/j.freeradbiomed.2021.10.025 .

Krunić, Matija, Ristić, Biljana, Bošnjak, Mihajlo, Paunović, Verica, Tovilović-Kovačević, Gordana, Zogović, Nevena, Mirčić, Aleksandar, Marković, Zoran, Todorović-Marković, Biljana, Jovanović, Svetlana, Kleut, Duška, Mojović, Miloš, Nakarada, Đura, Marković, Olivera, Vuković, Irena, Harhaji-Trajković, Ljubica, Trajković, Vladimir, "Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death." in Free Radical Biology and Medicine, 177 (2021):167-180,
https://doi.org/10.1016/j.freeradbiomed.2021.10.025 . .

TY  - JOUR
AU  - Krstić, Gordana
AU  - Stepanović, Ana
AU  - Jadranin, Milka
AU  - Pešić, Milica
AU  - Novaković, Miroslav
AU  - Aljančić, Ivana
AU  - Vajs, Vlatka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4752
AB  - In the previous study fifteen jatrophane diterpenes were isolated from the Euphorbia nicaeensis latex. Fourteen of them have been shown to be potent P-glycoprotein (P-gp) inhibitor in two MDR cancer cells (NCI-H460/R and DLD1-TxR). The aim of this study was to determine whether and which jatro­phane diterpenes can be isolated from the root of the plant, and then to examine their inhibition power on P-glycoprotein of selected cancer cell lines (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR and U87-TxR). Two previously undes­cribed jatrophane diterpenes were isolated from the root of E. nicaeensis col­lected in Deliblato Sand (Serbia). The structures of the isolated compounds were determined using 1D and 2D NMR, as well as HRESIMS data. The results obtained by MTT assay showed different antitumor potential of these two jatrophanes. Compound 1 inhibited cell growth of non-small cell lung car­cinoma cell lines NCI-H460 and NCI-H460/R, as well as glioblastoma cell lines U87 and U87-TxR, while jatrophane 2 was almost completely inactive in the suppression of cancer cell growth in a given range of concentrations. The obtained results also showed that the isolated compounds have an inhibitory effect on P-glycoprotein, as well as that their inhibitory potential is similar.
PB  - Belgrade : Serbian Chemical Society
T2  - The Journal of the Serbian Chemical Society
T1  - Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis
IS  - 12
VL  - 86
DO  - 10.2298/JSC210806085K
SP  - 1219
EP  - 1228
ER  - 

@article{
author = "Krstić, Gordana and Stepanović, Ana and Jadranin, Milka and Pešić, Milica and Novaković, Miroslav and Aljančić, Ivana and Vajs, Vlatka",
year = "2021",
abstract = "In the previous study fifteen jatrophane diterpenes were isolated from the Euphorbia nicaeensis latex. Fourteen of them have been shown to be potent P-glycoprotein (P-gp) inhibitor in two MDR cancer cells (NCI-H460/R and DLD1-TxR). The aim of this study was to determine whether and which jatro­phane diterpenes can be isolated from the root of the plant, and then to examine their inhibition power on P-glycoprotein of selected cancer cell lines (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR and U87-TxR). Two previously undes­cribed jatrophane diterpenes were isolated from the root of E. nicaeensis col­lected in Deliblato Sand (Serbia). The structures of the isolated compounds were determined using 1D and 2D NMR, as well as HRESIMS data. The results obtained by MTT assay showed different antitumor potential of these two jatrophanes. Compound 1 inhibited cell growth of non-small cell lung car­cinoma cell lines NCI-H460 and NCI-H460/R, as well as glioblastoma cell lines U87 and U87-TxR, while jatrophane 2 was almost completely inactive in the suppression of cancer cell growth in a given range of concentrations. The obtained results also showed that the isolated compounds have an inhibitory effect on P-glycoprotein, as well as that their inhibitory potential is similar.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "The Journal of the Serbian Chemical Society",
title = "Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis",
number = "12",
volume = "86",
doi = "10.2298/JSC210806085K",
pages = "1219-1228"
}

Krstić, G., Stepanović, A., Jadranin, M., Pešić, M., Novaković, M., Aljančić, I.,& Vajs, V.. (2021). Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis. in The Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 86(12), 1219-1228.
https://doi.org/10.2298/JSC210806085K

Krstić G, Stepanović A, Jadranin M, Pešić M, Novaković M, Aljančić I, Vajs V. Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis. in The Journal of the Serbian Chemical Society. 2021;86(12):1219-1228.
doi:10.2298/JSC210806085K .

Krstić, Gordana, Stepanović, Ana, Jadranin, Milka, Pešić, Milica, Novaković, Miroslav, Aljančić, Ivana, Vajs, Vlatka, "Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis" in The Journal of the Serbian Chemical Society, 86, no. 12 (2021):1219-1228,
https://doi.org/10.2298/JSC210806085K . .

TY  - CONF
AU  - Pergal, Marija
AU  - Brkljačić, Jelena
AU  - Tovilović-Kovačević, Gordana
AU  - Špirkova, Milena
AU  - Kodranov, Igor
AU  - Manojlović, Dragan
AU  - Knežević, Nenad
PY  - 2021
UR  - http://www.socphyschemserb.org/sr/publikacije/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5732
AB  - Surface characteristics and biocompatibility of new nanocomposites based on polyurethane network
and mesoporous silica nanoparticles (PUMSNs) were investigated. Surface topography and
roughness coefficient were studied by AFM. Biocompatibility with endothelial cells and cytotoxicity
of the PUNs were assessed using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium
bromide) and cell adhesion assays. The addition of MSNs into polyurethane network led to
improvement of surface properties, as well as exhibited promising characteristics regarding adhesion
of cells, toward potential application in coating for medical devices.
PB  - Belgrade: Society of Physical Chemists of Serbia
C3  - Proceedings:the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction: Physical Chemistry 2021. Vol. 2; 2021 Sep 20-24; Belgrade, Serbia
T1  - Cell adhesion characteristics of polyurethane-mesoporous silica nanoparticle composite materials
SP  - 480
EP  - 482
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5732
ER  - 

@conference{
author = "Pergal, Marija and Brkljačić, Jelena and Tovilović-Kovačević, Gordana and Špirkova, Milena and Kodranov, Igor and Manojlović, Dragan and Knežević, Nenad",
year = "2021",
abstract = "Surface characteristics and biocompatibility of new nanocomposites based on polyurethane network
and mesoporous silica nanoparticles (PUMSNs) were investigated. Surface topography and
roughness coefficient were studied by AFM. Biocompatibility with endothelial cells and cytotoxicity
of the PUNs were assessed using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium
bromide) and cell adhesion assays. The addition of MSNs into polyurethane network led to
improvement of surface properties, as well as exhibited promising characteristics regarding adhesion
of cells, toward potential application in coating for medical devices.",
publisher = "Belgrade: Society of Physical Chemists of Serbia",
journal = "Proceedings:the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction: Physical Chemistry 2021. Vol. 2; 2021 Sep 20-24; Belgrade, Serbia",
title = "Cell adhesion characteristics of polyurethane-mesoporous silica nanoparticle composite materials",
pages = "480-482",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5732"
}

Pergal, M., Brkljačić, J., Tovilović-Kovačević, G., Špirkova, M., Kodranov, I., Manojlović, D.,& Knežević, N.. (2021). Cell adhesion characteristics of polyurethane-mesoporous silica nanoparticle composite materials. in Proceedings:the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction: Physical Chemistry 2021. Vol. 2; 2021 Sep 20-24; Belgrade, Serbia
Belgrade: Society of Physical Chemists of Serbia., 480-482.
https://hdl.handle.net/21.15107/rcub_ibiss_5732

Pergal M, Brkljačić J, Tovilović-Kovačević G, Špirkova M, Kodranov I, Manojlović D, Knežević N. Cell adhesion characteristics of polyurethane-mesoporous silica nanoparticle composite materials. in Proceedings:the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction: Physical Chemistry 2021. Vol. 2; 2021 Sep 20-24; Belgrade, Serbia. 2021;:480-482.
https://hdl.handle.net/21.15107/rcub_ibiss_5732 .

Pergal, Marija, Brkljačić, Jelena, Tovilović-Kovačević, Gordana, Špirkova, Milena, Kodranov, Igor, Manojlović, Dragan, Knežević, Nenad, "Cell adhesion characteristics of polyurethane-mesoporous silica nanoparticle composite materials" in Proceedings:the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction: Physical Chemistry 2021. Vol. 2; 2021 Sep 20-24; Belgrade, Serbia (2021):480-482,
https://hdl.handle.net/21.15107/rcub_ibiss_5732 .

TY  - JOUR
AU  - Djukić, Nikola N.
AU  - Vasiljević, Božica
AU  - Milosevic, Djuradj
AU  - Valjarević, Aleksandar Dj
AU  - Jakšić, Tatjana R.
AU  - Vasić, Predrag S.
AU  - Štrbac, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4142
AB  - The study is focused on diatom communities as indicators of the water quality of aquatic ecosystems. The study included watercourses of the Timok River Basin in eastern Serbia. Sampling of algological material and physicochemical measurements were conducted at 30 locations in September 2016. Non-metric multidimensional scaling (NMDS) showed that conductivity had an effect on the spatial variability of the diatom communities. At locations under influence of acid mine drainage along the Borska river, its tributaries and the Timok river, the diversity of benthic diatoms decreased and the abundance of metal tolerant taxa Achnanthidium minutissimum, Nitzschia capitellata and Nitzschia palea increased. Our study revealed that the combined effect of different pollutants significantly impacted on diatom assemblages; diatoms are good bioindicators of multiple pressures; and diatom indices with different types of pollution, may show an unreliable picture of the actual state, therefore, biological and physico-chemical parameters should also be observed when interpreting the results of a solely diatom-based assessment of the ecological condition of freshwaters.
PB  - Academic Publishing House
T2  - Comptes Rendus de L'Academie Bulgare des Sciences
T2  - Comptes Rendus de L'Academie Bulgare des Sciences
T1  - A water quality assessment based on benthic diatoms of the timok river basin (Eastern Serbia) under multiple anthropogenic pressures
IS  - 12
VL  - 73
DO  - 10.7546/CRABS.2020.12.09
SP  - 1696
EP  - 1702
ER  - 

@article{
author = "Djukić, Nikola N. and Vasiljević, Božica and Milosevic, Djuradj and Valjarević, Aleksandar Dj and Jakšić, Tatjana R. and Vasić, Predrag S. and Štrbac, Snežana",
year = "2021",
abstract = "The study is focused on diatom communities as indicators of the water quality of aquatic ecosystems. The study included watercourses of the Timok River Basin in eastern Serbia. Sampling of algological material and physicochemical measurements were conducted at 30 locations in September 2016. Non-metric multidimensional scaling (NMDS) showed that conductivity had an effect on the spatial variability of the diatom communities. At locations under influence of acid mine drainage along the Borska river, its tributaries and the Timok river, the diversity of benthic diatoms decreased and the abundance of metal tolerant taxa Achnanthidium minutissimum, Nitzschia capitellata and Nitzschia palea increased. Our study revealed that the combined effect of different pollutants significantly impacted on diatom assemblages; diatoms are good bioindicators of multiple pressures; and diatom indices with different types of pollution, may show an unreliable picture of the actual state, therefore, biological and physico-chemical parameters should also be observed when interpreting the results of a solely diatom-based assessment of the ecological condition of freshwaters.",
publisher = "Academic Publishing House",
journal = "Comptes Rendus de L'Academie Bulgare des Sciences, Comptes Rendus de L'Academie Bulgare des Sciences",
title = "A water quality assessment based on benthic diatoms of the timok river basin (Eastern Serbia) under multiple anthropogenic pressures",
number = "12",
volume = "73",
doi = "10.7546/CRABS.2020.12.09",
pages = "1696-1702"
}

Djukić, N. N., Vasiljević, B., Milosevic, D., Valjarević, A. D., Jakšić, T. R., Vasić, P. S.,& Štrbac, S.. (2021). A water quality assessment based on benthic diatoms of the timok river basin (Eastern Serbia) under multiple anthropogenic pressures. in Comptes Rendus de L'Academie Bulgare des Sciences
Academic Publishing House., 73(12), 1696-1702.
https://doi.org/10.7546/CRABS.2020.12.09

Djukić NN, Vasiljević B, Milosevic D, Valjarević AD, Jakšić TR, Vasić PS, Štrbac S. A water quality assessment based on benthic diatoms of the timok river basin (Eastern Serbia) under multiple anthropogenic pressures. in Comptes Rendus de L'Academie Bulgare des Sciences. 2021;73(12):1696-1702.
doi:10.7546/CRABS.2020.12.09 .

Djukić, Nikola N., Vasiljević, Božica, Milosevic, Djuradj, Valjarević, Aleksandar Dj, Jakšić, Tatjana R., Vasić, Predrag S., Štrbac, Snežana, "A water quality assessment based on benthic diatoms of the timok river basin (Eastern Serbia) under multiple anthropogenic pressures" in Comptes Rendus de L'Academie Bulgare des Sciences, 73, no. 12 (2021):1696-1702,
https://doi.org/10.7546/CRABS.2020.12.09 . .

TY  - JOUR
AU  - Drača, Dijana
AU  - Edeler, David
AU  - Saoud, Mohamad
AU  - Dojčinović, Biljana
AU  - Dunđerović, Duško
AU  - Đmura, Goran
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2021
UR  - internal-pdf://Drača et al. - 2021 - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells i.pdf
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0162013421000301
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33582397
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4154
AB  - CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.
PB  - Elsevier BV
T2  - Journal of Inorganic Biochemistry
T1  - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.
VL  - 217
DO  - 10.1016/j.jinorgbio.2021.111383
SP  - 111383
ER  - 

@article{
author = "Drača, Dijana and Edeler, David and Saoud, Mohamad and Dojčinović, Biljana and Dunđerović, Duško and Đmura, Goran and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2021",
abstract = "CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.",
publisher = "Elsevier BV",
journal = "Journal of Inorganic Biochemistry",
title = "Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.",
volume = "217",
doi = "10.1016/j.jinorgbio.2021.111383",
pages = "111383"
}

Drača, D., Edeler, D., Saoud, M., Dojčinović, B., Dunđerović, D., Đmura, G., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2021). Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.. in Journal of Inorganic Biochemistry
Elsevier BV., 217, 111383.
https://doi.org/10.1016/j.jinorgbio.2021.111383

Drača D, Edeler D, Saoud M, Dojčinović B, Dunđerović D, Đmura G, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.. in Journal of Inorganic Biochemistry. 2021;217:111383.
doi:10.1016/j.jinorgbio.2021.111383 .

Drača, Dijana, Edeler, David, Saoud, Mohamad, Dojčinović, Biljana, Dunđerović, Duško, Đmura, Goran, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies." in Journal of Inorganic Biochemistry, 217 (2021):111383,
https://doi.org/10.1016/j.jinorgbio.2021.111383 . .

TY  - JOUR
AU  - Pergal, Marija V.
AU  - Brkljačić, Jelena
AU  - Tovilović-Kovačević, Gordana
AU  - Špírkova, Milena
AU  - Kodranov, Igor D.
AU  - Manojlović, Dragan D.
AU  - Ostojić, Sanja
AU  - Knežević, Nikola Ž.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4161
AB  - Novel polyurethane nanocomposite (PUN) materials containing different surface-functionalized mesoporous silica nanoparticles (MSNs) were prepared by in situ polymerization methodology. Polyurethane network was formed from poly (dimethylsiloxane)-based macrodiol (PDMS), 4, 4′ -methylenediphenyldiisocyanate (MDI), and hyperbranched polyester of the second pseudo-generation (BH-20; used as crosslinking agent). PU and PU/MSN nanocomposites contained equal ratios of soft PDMS and hard MDI-BH-20 segments. Non-functionalized and surface-functionalized (with 3-(trihydroxysilyl) propyl methylphosphonate (FOMSN) and 2-[methoxy (polyethyleneoxy) 6-9propyl] trimethoxysilane (PEGMSN)) MSNs were used as the nanofillers at a concentration of 1 wt%. Prepared materials were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic mechanical thermal analyses (DMTA), nanoindentation, equilibrium swelling and water absorption measurements. Characteristics of the prepared PUNs when in contact with a biological environment were assessed through testing their biocompatibility, protein adsorption and adhesion of endothelial cells. The favourable influence of MSNs on the physico-chemical and biological characteristics of these novel PUN materials was identified, which evidences their vast applicability potential as coatings for medical devices and implants.
PB  - Elsevier
T2  - Progress in Organic Coatings
T1  - Effect of mesoporous silica nanoparticles on the properties of polyurethane network composites
VL  - 151
DO  - 10.1016/j.porgcoat.2020.106049
SP  - 106049
ER  - 

@article{
author = "Pergal, Marija V. and Brkljačić, Jelena and Tovilović-Kovačević, Gordana and Špírkova, Milena and Kodranov, Igor D. and Manojlović, Dragan D. and Ostojić, Sanja and Knežević, Nikola Ž.",
year = "2021",
abstract = "Novel polyurethane nanocomposite (PUN) materials containing different surface-functionalized mesoporous silica nanoparticles (MSNs) were prepared by in situ polymerization methodology. Polyurethane network was formed from poly (dimethylsiloxane)-based macrodiol (PDMS), 4, 4′ -methylenediphenyldiisocyanate (MDI), and hyperbranched polyester of the second pseudo-generation (BH-20; used as crosslinking agent). PU and PU/MSN nanocomposites contained equal ratios of soft PDMS and hard MDI-BH-20 segments. Non-functionalized and surface-functionalized (with 3-(trihydroxysilyl) propyl methylphosphonate (FOMSN) and 2-[methoxy (polyethyleneoxy) 6-9propyl] trimethoxysilane (PEGMSN)) MSNs were used as the nanofillers at a concentration of 1 wt%. Prepared materials were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic mechanical thermal analyses (DMTA), nanoindentation, equilibrium swelling and water absorption measurements. Characteristics of the prepared PUNs when in contact with a biological environment were assessed through testing their biocompatibility, protein adsorption and adhesion of endothelial cells. The favourable influence of MSNs on the physico-chemical and biological characteristics of these novel PUN materials was identified, which evidences their vast applicability potential as coatings for medical devices and implants.",
publisher = "Elsevier",
journal = "Progress in Organic Coatings",
title = "Effect of mesoporous silica nanoparticles on the properties of polyurethane network composites",
volume = "151",
doi = "10.1016/j.porgcoat.2020.106049",
pages = "106049"
}

Pergal, M. V., Brkljačić, J., Tovilović-Kovačević, G., Špírkova, M., Kodranov, I. D., Manojlović, D. D., Ostojić, S.,& Knežević, N. Ž.. (2021). Effect of mesoporous silica nanoparticles on the properties of polyurethane network composites. in Progress in Organic Coatings
Elsevier., 151, 106049.
https://doi.org/10.1016/j.porgcoat.2020.106049

Pergal MV, Brkljačić J, Tovilović-Kovačević G, Špírkova M, Kodranov ID, Manojlović DD, Ostojić S, Knežević NŽ. Effect of mesoporous silica nanoparticles on the properties of polyurethane network composites. in Progress in Organic Coatings. 2021;151:106049.
doi:10.1016/j.porgcoat.2020.106049 .

Pergal, Marija V., Brkljačić, Jelena, Tovilović-Kovačević, Gordana, Špírkova, Milena, Kodranov, Igor D., Manojlović, Dragan D., Ostojić, Sanja, Knežević, Nikola Ž., "Effect of mesoporous silica nanoparticles on the properties of polyurethane network composites" in Progress in Organic Coatings, 151 (2021):106049,
https://doi.org/10.1016/j.porgcoat.2020.106049 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/11/1719
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4653
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3
IS  - 11
VL  - 10
DO  - 10.3390/antiox10111719
SP  - 1719
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
year = "2021",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3",
number = "11",
volume = "10",
doi = "10.3390/antiox10111719",
pages = "1719"
}

Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants
Basel: MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719

Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .

Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3" in Antioxidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .