TY  - JOUR
AU  - Brkljača, Nemanja
AU  - Đurović, Saša
AU  - Milošević, Sanja
AU  - Gašić, Uroš
AU  - Panković, Dejana
AU  - Zeković, Zoran
AU  - Pavlić, Branimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6469
AB  - Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towards the use of green extraction techniques and maximum utilization of raw material. In that regard conventional (hydrodistillation – HD) and novel extraction techniques (microwave-assisted hydrodistillation – MWHD, supercritical fluid extraction – SFE and microwave-assisted extraction – MAE) were applied for isolating various fractions (essential oil, lipid extracts and liquid extract) with promising bioactive potential. Essential oils and lipid extracts were characterized regarding yield, terpenoid profile determined by GC-MS and in vitro antioxidant activity towards DPPH and ABTS + radicals. Furthermore, the content of two major cannabinoids was determined in plant material and in extract obtained by SFE. Sequential SFE technique allows obtaining lipid extracts in the first step of process as well as raffinates after SFE in the second step. MAE was applied for isolation of polyphenolic fraction from raw materials and SFE raffinates. Polyphenolic content was determined by spectrophotometric assays and UHPLC-Triple-quadrupole-MS. The results suggested that hemp is an excellent source of valuable terpenoid, cannabinoid and polyphenolic antioxidant agents, and further research should be focused on their utilization in dietary supplements and functional foods.
PB  - Elsevier
T2  - Sustainable Chemistry and Pharmacy
T1  - Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds
VL  - 35
DO  - 10.1016/j.scp.2023.101213
SP  - 101213
ER  - 

@article{
author = "Brkljača, Nemanja and Đurović, Saša and Milošević, Sanja and Gašić, Uroš and Panković, Dejana and Zeković, Zoran and Pavlić, Branimir",
year = "2023",
abstract = "Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towards the use of green extraction techniques and maximum utilization of raw material. In that regard conventional (hydrodistillation – HD) and novel extraction techniques (microwave-assisted hydrodistillation – MWHD, supercritical fluid extraction – SFE and microwave-assisted extraction – MAE) were applied for isolating various fractions (essential oil, lipid extracts and liquid extract) with promising bioactive potential. Essential oils and lipid extracts were characterized regarding yield, terpenoid profile determined by GC-MS and in vitro antioxidant activity towards DPPH and ABTS + radicals. Furthermore, the content of two major cannabinoids was determined in plant material and in extract obtained by SFE. Sequential SFE technique allows obtaining lipid extracts in the first step of process as well as raffinates after SFE in the second step. MAE was applied for isolation of polyphenolic fraction from raw materials and SFE raffinates. Polyphenolic content was determined by spectrophotometric assays and UHPLC-Triple-quadrupole-MS. The results suggested that hemp is an excellent source of valuable terpenoid, cannabinoid and polyphenolic antioxidant agents, and further research should be focused on their utilization in dietary supplements and functional foods.",
publisher = "Elsevier",
journal = "Sustainable Chemistry and Pharmacy",
title = "Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds",
volume = "35",
doi = "10.1016/j.scp.2023.101213",
pages = "101213"
}

Brkljača, N., Đurović, S., Milošević, S., Gašić, U., Panković, D., Zeković, Z.,& Pavlić, B.. (2023). Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds. in Sustainable Chemistry and Pharmacy
Elsevier., 35, 101213.
https://doi.org/10.1016/j.scp.2023.101213

Brkljača N, Đurović S, Milošević S, Gašić U, Panković D, Zeković Z, Pavlić B. Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds. in Sustainable Chemistry and Pharmacy. 2023;35:101213.
doi:10.1016/j.scp.2023.101213 .

Brkljača, Nemanja, Đurović, Saša, Milošević, Sanja, Gašić, Uroš, Panković, Dejana, Zeković, Zoran, Pavlić, Branimir, "Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds" in Sustainable Chemistry and Pharmacy, 35 (2023):101213,
https://doi.org/10.1016/j.scp.2023.101213 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6467
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
SP  - 477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477",
pages = "477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae
Basel: MDPI., 9(4), 477.
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4):477.
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023):477,
https://doi.org/10.3390/horticulturae9040477 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Stupar, Alena
AU  - Uysal, Ahmet
AU  - Şenkardes, Ismail
AU  - Sinan, Kouadio Ibrahime
AU  - Picot-Allain, Marie Carene Nancy
AU  - Ak, Gunes
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3769
AB  - The growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction
methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach.
The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic,
antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using
conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted
(UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical char-
acterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass
spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested
extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE
(170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid
content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS
(679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme in-
hibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent
[GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent
[KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial
properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive com-
pounds from Origanum vulgare subsp. viridulum aerial parts.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods
VL  - 154
DO  - 10.1016/j.indcrop.2020.112747
SP  - 112747
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Dragićević, Milan and Stupar, Alena and Uysal, Ahmet and Şenkardes, Ismail and Sinan, Kouadio Ibrahime and Picot-Allain, Marie Carene Nancy and Ak, Gunes and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "The growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction
methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach.
The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic,
antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using
conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted
(UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical char-
acterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass
spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested
extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE
(170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid
content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS
(679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme in-
hibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent
[GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent
[KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial
properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive com-
pounds from Origanum vulgare subsp. viridulum aerial parts.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods",
volume = "154",
doi = "10.1016/j.indcrop.2020.112747",
pages = "112747"
}

Zengin, G., Cvetanović, A., Gašić, U., Dragićević, M., Stupar, A., Uysal, A., Şenkardes, I., Sinan, K. I., Picot-Allain, M. C. N., Ak, G.,& Mahomoodally, M. F.. (2020). UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods. in Industrial Crops & Products
Elsevier., 154, 112747.
https://doi.org/10.1016/j.indcrop.2020.112747

Zengin G, Cvetanović A, Gašić U, Dragićević M, Stupar A, Uysal A, Şenkardes I, Sinan KI, Picot-Allain MCN, Ak G, Mahomoodally MF. UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods. in Industrial Crops & Products. 2020;154:112747.
doi:10.1016/j.indcrop.2020.112747 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Dragićević, Milan, Stupar, Alena, Uysal, Ahmet, Şenkardes, Ismail, Sinan, Kouadio Ibrahime, Picot-Allain, Marie Carene Nancy, Ak, Gunes, Mahomoodally, Mohamad Fawzi, "UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods" in Industrial Crops & Products, 154 (2020):112747,
https://doi.org/10.1016/j.indcrop.2020.112747 . .