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A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity

Kostić, Marina; Ivanov, Marija; Fernandes, Ângela; Calhelha, Ricardo C.; Glamočlija, Jasmina; Barros, Lillian; Soković, Marina; Ćirić, Ana

(Basel: MDPI, 2023)

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - https://www.mdpi.com/2309-608X/9/1/70
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9864295
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5461
AB  - Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity
IS  - 1
VL  - 9
DO  - 10.3390/jof9010070
SP  - 70
ER  - 
@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Soković, Marina and Ćirić, Ana",
year = "2023",
abstract = "Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity",
number = "1",
volume = "9",
doi = "10.3390/jof9010070",
pages = "70"
}
Kostić, M., Ivanov, M., Fernandes, Â., Calhelha, R. C., Glamočlija, J., Barros, L., Soković, M.,& Ćirić, A.. (2023). A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi
Basel: MDPI., 9(1), 70.
https://doi.org/10.3390/jof9010070
Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Soković M, Ćirić A. A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi. 2023;9(1):70.
doi:10.3390/jof9010070 .
Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Soković, Marina, Ćirić, Ana, "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity" in Journal of Fungi, 9, no. 1 (2023):70,
https://doi.org/10.3390/jof9010070 . .
1

Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice

da Silva, Tamires Barlati Vieira; Dias, Maria Inês; Pereira, Carla; Mandim, Filipa; Ivanov, Marija; Soković, Marina; Ferreira, Isabel C. F. R.; Barros, Lillian; Seixas, Flávio Augusto Vicente; Bracht, Adelar; Peralta, Rosane Marina

(2023)

TY  - JOUR
AU  - da Silva, Tamires Barlati Vieira
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Mandim, Filipa
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Seixas, Flávio Augusto Vicente
AU  - Bracht, Adelar
AU  - Peralta, Rosane Marina
PY  - 2023
UR  - http://xlink.rsc.org/?DOI=D2FO02442J
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36723015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5465
AB  - A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.
T2  - Food & Function
T1  - Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice
IS  - 3
VL  - 14
DO  - 10.1039/d2fo02442j
SP  - 1761
EP  - 1772
ER  - 
@article{
author = "da Silva, Tamires Barlati Vieira and Dias, Maria Inês and Pereira, Carla and Mandim, Filipa and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Seixas, Flávio Augusto Vicente and Bracht, Adelar and Peralta, Rosane Marina",
year = "2023",
abstract = "A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.",
journal = "Food & Function",
title = "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice",
number = "3",
volume = "14",
doi = "10.1039/d2fo02442j",
pages = "1761-1772"
}
da Silva, T. B. V., Dias, M. I., Pereira, C., Mandim, F., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Barros, L., Seixas, F. A. V., Bracht, A.,& Peralta, R. M.. (2023). Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function, 14(3), 1761-1772.
https://doi.org/10.1039/d2fo02442j
da Silva TBV, Dias MI, Pereira C, Mandim F, Ivanov M, Soković M, Ferreira ICFR, Barros L, Seixas FAV, Bracht A, Peralta RM. Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function. 2023;14(3):1761-1772.
doi:10.1039/d2fo02442j .
da Silva, Tamires Barlati Vieira, Dias, Maria Inês, Pereira, Carla, Mandim, Filipa, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Seixas, Flávio Augusto Vicente, Bracht, Adelar, Peralta, Rosane Marina, "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice" in Food & Function, 14, no. 3 (2023):1761-1772,
https://doi.org/10.1039/d2fo02442j . .
1
2
2

Preservation of Chocolate Muffins with Lemon Balm, Oregano, and Rosemary Extracts

Pedrosa, Mariana C.; Ueda, Jonata M.; Melgar, Bruno; Dias, Maria Inês; Pinela, José; Calhelha, Ricardo C.; Ivanov, Marija; Soković, Marina; Heleno, Sandrina; Silva, Aline Bruna da; Carocho, Márcio; Ferreira, Isabel C. F. R.; Barros, Lillian

(MDPI AG, 2021)

TY  - JOUR
AU  - Pedrosa, Mariana C.
AU  - Ueda, Jonata M.
AU  - Melgar, Bruno
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Heleno, Sandrina
AU  - Silva, Aline Bruna da
AU  - Carocho, Márcio
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2304-8158/10/1/165
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4177
AB  - Muffins are snacks made from flour and chocolate and preserved with synthetic additives. Following consumer trends, the search for natural food additives has gained traction. Plants such as rosemary, lemon balm, and oregano were analyzed following an optimization of ultrasound assisted extraction, screened for their antioxidant and antimicrobial activity and incorporated in chocolate muffins, comparing them to synthetic preservatives over the course of 8 days. The nutritional profile, organic and fatty acids, soluble sugars, texture profile, external color and digital imaging of the muffin pores were analyzed. Slight changes were sought for the muffins incorporated with the natural extracts. By means of linear discriminant analysis, rosemary extract was considered the most promising extract to preserve the muffins due to its similarity to potassium sorbate, showing no changes in the muffins it was incorporated in, although it showed a lower amount of phenolic compounds when compared to lemon balm.
PB  - MDPI AG
T2  - Foods
T1  - Preservation of Chocolate Muffins with Lemon Balm, Oregano, and Rosemary Extracts
IS  - 1
VL  - 10
DO  - 10.3390/foods10010165
SP  - 165
ER  - 
@article{
author = "Pedrosa, Mariana C. and Ueda, Jonata M. and Melgar, Bruno and Dias, Maria Inês and Pinela, José and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Heleno, Sandrina and Silva, Aline Bruna da and Carocho, Márcio and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Muffins are snacks made from flour and chocolate and preserved with synthetic additives. Following consumer trends, the search for natural food additives has gained traction. Plants such as rosemary, lemon balm, and oregano were analyzed following an optimization of ultrasound assisted extraction, screened for their antioxidant and antimicrobial activity and incorporated in chocolate muffins, comparing them to synthetic preservatives over the course of 8 days. The nutritional profile, organic and fatty acids, soluble sugars, texture profile, external color and digital imaging of the muffin pores were analyzed. Slight changes were sought for the muffins incorporated with the natural extracts. By means of linear discriminant analysis, rosemary extract was considered the most promising extract to preserve the muffins due to its similarity to potassium sorbate, showing no changes in the muffins it was incorporated in, although it showed a lower amount of phenolic compounds when compared to lemon balm.",
publisher = "MDPI AG",
journal = "Foods",
title = "Preservation of Chocolate Muffins with Lemon Balm, Oregano, and Rosemary Extracts",
number = "1",
volume = "10",
doi = "10.3390/foods10010165",
pages = "165"
}
Pedrosa, M. C., Ueda, J. M., Melgar, B., Dias, M. I., Pinela, J., Calhelha, R. C., Ivanov, M., Soković, M., Heleno, S., Silva, A. B. d., Carocho, M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Preservation of Chocolate Muffins with Lemon Balm, Oregano, and Rosemary Extracts. in Foods
MDPI AG., 10(1), 165.
https://doi.org/10.3390/foods10010165
Pedrosa MC, Ueda JM, Melgar B, Dias MI, Pinela J, Calhelha RC, Ivanov M, Soković M, Heleno S, Silva ABD, Carocho M, Ferreira ICFR, Barros L. Preservation of Chocolate Muffins with Lemon Balm, Oregano, and Rosemary Extracts. in Foods. 2021;10(1):165.
doi:10.3390/foods10010165 .
Pedrosa, Mariana C., Ueda, Jonata M., Melgar, Bruno, Dias, Maria Inês, Pinela, José, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Heleno, Sandrina, Silva, Aline Bruna da, Carocho, Márcio, Ferreira, Isabel C. F. R., Barros, Lillian, "Preservation of Chocolate Muffins with Lemon Balm, Oregano, and Rosemary Extracts" in Foods, 10, no. 1 (2021):165,
https://doi.org/10.3390/foods10010165 . .
3
1
2

Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.

Mandim, Filipa; Petropoulos, Spyridon A.; Dias, Maria Inês; Pinela, José; Kostić, Marina; Soković, Marina; Santos-Buelga, Celestino; Ferreira, Isabel C. F. R.; Barros, Lillian

(Basel: MDPI, 2021)

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/12/1907
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8750300
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4741
AB  - Hydroethanolic extracts of cardoon petioles collected at sixteen growth stages (P1-P16) were characterized in terms of their phenolic composition and bioactive potential (antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities). Fifteen phenolic compounds were tentatively identified (i.e., ten phenolic acids and five flavonoid glycosides); the main compounds were 5-O-caffeoylquinic and 1,5-di-O-caffeoylquinic acids. Samples collected at early maturity (P1-P4) presented a weak positive correlation between the higher content in polyphenols (P3: 101-mg/g extract) and better inhibition capacity against thiobarbituric acid reactive substance formation (TBARS; P3: IC50 = 5.0 µg/mL). Samples at intermediate maturation stages (P9) presented higher cytotoxic and anti-inflammatory potential. Moreover, immature petioles showed greater antihemolytic (OxHLIA; P4: IC50 = 65 and 180 µg/mL for Δt of 60 and 120 min, respectively) and antibacterial activity. The antifungal activity varied depending on the maturation stage and the fungi strain. In conclusion, the maturation stage may greatly affect the polyphenols composition and content and the bioactive potential of cardoon petioles.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.
IS  - 12
VL  - 10
DO  - 10.3390/antiox10121907
SP  - 1907
ER  - 
@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Hydroethanolic extracts of cardoon petioles collected at sixteen growth stages (P1-P16) were characterized in terms of their phenolic composition and bioactive potential (antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities). Fifteen phenolic compounds were tentatively identified (i.e., ten phenolic acids and five flavonoid glycosides); the main compounds were 5-O-caffeoylquinic and 1,5-di-O-caffeoylquinic acids. Samples collected at early maturity (P1-P4) presented a weak positive correlation between the higher content in polyphenols (P3: 101-mg/g extract) and better inhibition capacity against thiobarbituric acid reactive substance formation (TBARS; P3: IC50 = 5.0 µg/mL). Samples at intermediate maturation stages (P9) presented higher cytotoxic and anti-inflammatory potential. Moreover, immature petioles showed greater antihemolytic (OxHLIA; P4: IC50 = 65 and 180 µg/mL for Δt of 60 and 120 min, respectively) and antibacterial activity. The antifungal activity varied depending on the maturation stage and the fungi strain. In conclusion, the maturation stage may greatly affect the polyphenols composition and content and the bioactive potential of cardoon petioles.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.",
number = "12",
volume = "10",
doi = "10.3390/antiox10121907",
pages = "1907"
}
Mandim, F., Petropoulos, S. A., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 10(12), 1907.
https://doi.org/10.3390/antiox10121907
Mandim F, Petropoulos SA, Dias MI, Pinela J, Kostić M, Soković M, Santos-Buelga C, Ferreira ICFR, Barros L. Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.. in Antioxidants (Basel, Switzerland). 2021;10(12):1907.
doi:10.3390/antiox10121907 .
Mandim, Filipa, Petropoulos, Spyridon A., Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C. F. R., Barros, Lillian, "Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage." in Antioxidants (Basel, Switzerland), 10, no. 12 (2021):1907,
https://doi.org/10.3390/antiox10121907 . .
11
10

Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina

Ivković, Sanja; Jovanović Macura, Irena; Antonijević, Tijana; Kanazir, Selma; Henrique, Domingos

(2019)

TY  - JOUR
AU  - Ivković, Sanja
AU  - Jovanović Macura, Irena
AU  - Antonijević, Tijana
AU  - Kanazir, Selma
AU  - Henrique, Domingos
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4389
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3648
AB  - Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)-or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Muller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Muller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin-in either control or EGFsupplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.
T2  - Archives of Biological Sciences
T1  - Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina
IS  - 4
VL  - 71
DO  - 10.2298/abs190617054i
SP  - 711
EP  - 719
ER  - 
@article{
author = "Ivković, Sanja and Jovanović Macura, Irena and Antonijević, Tijana and Kanazir, Selma and Henrique, Domingos",
year = "2019",
abstract = "Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)-or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Muller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Muller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin-in either control or EGFsupplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.",
journal = "Archives of Biological Sciences",
title = "Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina",
number = "4",
volume = "71",
doi = "10.2298/abs190617054i",
pages = "711-719"
}
Ivković, S., Jovanović Macura, I., Antonijević, T., Kanazir, S.,& Henrique, D.. (2019). Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. in Archives of Biological Sciences, 71(4), 711-719.
https://doi.org/10.2298/abs190617054i
Ivković S, Jovanović Macura I, Antonijević T, Kanazir S, Henrique D. Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. in Archives of Biological Sciences. 2019;71(4):711-719.
doi:10.2298/abs190617054i .
Ivković, Sanja, Jovanović Macura, Irena, Antonijević, Tijana, Kanazir, Selma, Henrique, Domingos, "Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina" in Archives of Biological Sciences, 71, no. 4 (2019):711-719,
https://doi.org/10.2298/abs190617054i . .