TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milošković, Aleksandra
AU  - Radojković, Nataša
AU  - Simić, Vladica
AU  - Dojčinović, Biljana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Timilijić, Jovana
AU  - Djordjević, Jelena
AU  - Žegura, Bojana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S004896971732805X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2887
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
T2  - Science of The Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
SP  - 1289
EP  - 1299
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Radojković, Nataša and Simić, Vladica and Dojčinović, Biljana and Kračun-Kolarević, Margareta and Paunović, Momir and Kostić, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka and Timilijić, Jovana and Djordjević, Jelena and Žegura, Bojana",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
journal = "Science of The Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099",
pages = "1289-1299"
}

Jovanović, J., Kolarević, S., Milošković, A., Radojković, N., Simić, V., Dojčinović, B., Kračun-Kolarević, M., Paunović, M., Kostić, J., Sunjog, K., Gačić, Z., Vuković-Gačić, B., Timilijić, J., Djordjević, J.,& Žegura, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of The Total Environment, 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milošković A, Radojković N, Simić V, Dojčinović B, Kračun-Kolarević M, Paunović M, Kostić J, Sunjog K, Gačić Z, Vuković-Gačić B, Timilijić J, Djordjević J, Žegura B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of The Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Radojković, Nataša, Simić, Vladica, Dojčinović, Biljana, Kračun-Kolarević, Margareta, Paunović, Momir, Kostić, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, Timilijić, Jovana, Djordjević, Jelena, Žegura, Bojana, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of The Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .