@conference{
author = "Đorđević, Marija and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Uskoković, Aleksandra and Grdović, Nevena and Dinić, Svetlana and Đorđević, Miloš and Tolić, Anja and Rajić, Jovana and Hunyadi, Attila and Vidaković, Melita",
year = "2018",
abstract = "Objective: 20-hydroxyecdysone (20HE), a steroid hormone that modulates molting response in insects exerts many pharmacological effects in mammals, most of which appear beneficial. The aim of this study was to investigate whether 20HE is able to reduce the destruction of beta-cells of the islets of Langerhans and ameliorate hyperglycemia induced changes in liver tissue in streptozotocin (STZ)-induced rat model of diabetes.
Methods: An experimental model of diabetes was induced in rats by the administration of 35 mg/kg STZ intraperitoneally for 4 consecutive days. 20HE was administered orally at a dose of20 mg/ kg body weight for four weeks, starting from the last day of STZ administration. Pancreas tissue sections were analyzed by hematoxylin and eosin staining and immunohistochemical staining with insulin. Estimation of oxidative damage of DNA and lipids in the liver were detected by comet assay and thiobarbituric acid-reactive substance assay, respectively. Liver sections were analyzed by hematoxylin/eosin and Masson's trichrome staining.
Results: Diabetic rats treated with the 20HE displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin. The administration of 20HE to diabetic rats also led to positive histological changes of pancreatic islets and increase in the number of insulin-positive cells in the islets which was accompanied by increased serum insulin level. These results show that 20HE administration to diabetic rats restrained islet destruction and partially restored the number of insulin-positive cells. In addition, treatment of 20HE attenuated diabetes-induced liver damage in rats according to lower level of DNA damage and reduction of oxidative damage oflipids. This result is in accordance with observed improvement of liver architecture in 20HE treated diabetic rats. Staining of collagen and increase in E-cadherin and decreases in a-smooth muscle actin revealed that administration of 20HE to diabetic rats attenuated fibrotic process in the liver.
Conclusions: 20HE administration seems to be beneficial for improving the hyperglycemia by increasing beta-cell mass and preventing diabetic complications in liver by attenuating fibrotic process.",
publisher = "Krager Publishers",
journal = "Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy",
title = "20-Hydroxyecdysone Protects Pancreatic Islets and Liver in Streptozotocin-lnduced Diabetic Rats",
doi = "10.1159/000490753",
pages = "14"
}