TY  - JOUR
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Jarić, Ivana
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2018
UR  - http://doi.wiley.com/10.1002/ar.23809
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3033
AB  - The aim of the study was to examine the potential of the principal soy isoflavones, genistein and daidzein, or isoflavone rich soy extract to recover pituitary castration cells in orchidectomized adult male rats in comparison with the effects of estradiol. Two weeks post orchidectomy (Orx), animals received estradiol‐dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham‐operated (So) and Orx rats received just the vehicle. Changes in the volumes of pars distalis, of individual follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design‐based stereology. The intracellular content of βFSH and βLH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of pars distalis, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol‐like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of pars distalis, decreased the volume density of LH‐labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH‐containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx‐induced changes in pituitary castration cells.
T2  - The Anatomical Record
T1  - Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study
IS  - 8
VL  - 301
DO  - 10.1002/ar.23809
SP  - 1416
EP  - 1425
ER  - 

@article{
author = "Nestorović, Nataša and Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Jarić, Ivana and Ristić, Nataša and Filipović, Branko and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2018",
abstract = "The aim of the study was to examine the potential of the principal soy isoflavones, genistein and daidzein, or isoflavone rich soy extract to recover pituitary castration cells in orchidectomized adult male rats in comparison with the effects of estradiol. Two weeks post orchidectomy (Orx), animals received estradiol‐dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham‐operated (So) and Orx rats received just the vehicle. Changes in the volumes of pars distalis, of individual follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design‐based stereology. The intracellular content of βFSH and βLH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of pars distalis, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol‐like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of pars distalis, decreased the volume density of LH‐labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH‐containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx‐induced changes in pituitary castration cells.",
journal = "The Anatomical Record",
title = "Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study",
number = "8",
volume = "301",
doi = "10.1002/ar.23809",
pages = "1416-1425"
}

Nestorović, N., Trifunović, S., Manojlović-Stojanoski, M., Jarić, I., Ristić, N., Filipović, B., Šošić-Jurjević, B.,& Milošević, V.. (2018). Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study. in The Anatomical Record, 301(8), 1416-1425.
https://doi.org/10.1002/ar.23809

Nestorović N, Trifunović S, Manojlović-Stojanoski M, Jarić I, Ristić N, Filipović B, Šošić-Jurjević B, Milošević V. Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study. in The Anatomical Record. 2018;301(8):1416-1425.
doi:10.1002/ar.23809 .

Nestorović, Nataša, Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Jarić, Ivana, Ristić, Nataša, Filipović, Branko, Šošić-Jurjević, Branka , Milošević, Verica, "Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study" in The Anatomical Record, 301, no. 8 (2018):1416-1425,
https://doi.org/10.1002/ar.23809 . .