TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .