TY  - JOUR
AU  - Tolić, Anja
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Đorđević, Miloš
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Poznanović, Goran
AU  - Jurkowski, Tomasz P.
AU  - Vidaković, Melita
AU  - Uskoković, Aleksandra
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3460
AB  - Previously, we described the link between C-X-C motif chemokine 12 (Cxcl12) gene induction
and DNA hypomethylation in the absence of poly(ADP-ribose) polymerase 1 (PARP-1). We have now
firmly established that demethylation is the primary cause of gene induction on the basis of Cxcl12 gene
upregulation upon treatment with the demethylating agent 5-azacytidine (5-aza). Since the demethylation
state of Cxcl12 is favored by PARP-1 absence, we investigated the presence of ten-eleven translocation
(TET) proteins on the Cxcl12 promoter in order to corroborate the relationship between the demethylation
process and increased gene expression that occurs in the absence of PARP-1. Analysis was performed on the promoter region within CpG islands of Cxcl12 from control mouse embryonic fibroblasts (NIH3T3)
and PARP-1 knock-out mouse embryonic fibroblasts (PARP1-/-). The lack of PARP-1 increased the abundance of TET2 on the Cxcl12 promoter, suggesting that TET-mediated demethylation provoked by the absence of PARP-1 could account for the observed increased expression of this chemokine. Deciphering the regulation of DNA (de)methylation factors that control Cxcl12 expression may provide an additional therapeutic approach in pharmacological interventions where gene switching on or off based on targeted stimulation or inhibition is necessary.
T2  - Archives of Biological Sciences
T1  - Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1
IS  - 3
VL  - 71
DO  - 10.2298/ABS190404027T
SP  - 455
EP  - 462
ER  - 

@article{
author = "Tolić, Anja and Rajić, Jovana and Đorđević, Marija and Đorđević, Miloš and Dinić, Svetlana and Grdović, Nevena and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Poznanović, Goran and Jurkowski, Tomasz P. and Vidaković, Melita and Uskoković, Aleksandra",
year = "2019",
abstract = "Previously, we described the link between C-X-C motif chemokine 12 (Cxcl12) gene induction
and DNA hypomethylation in the absence of poly(ADP-ribose) polymerase 1 (PARP-1). We have now
firmly established that demethylation is the primary cause of gene induction on the basis of Cxcl12 gene
upregulation upon treatment with the demethylating agent 5-azacytidine (5-aza). Since the demethylation
state of Cxcl12 is favored by PARP-1 absence, we investigated the presence of ten-eleven translocation
(TET) proteins on the Cxcl12 promoter in order to corroborate the relationship between the demethylation
process and increased gene expression that occurs in the absence of PARP-1. Analysis was performed on the promoter region within CpG islands of Cxcl12 from control mouse embryonic fibroblasts (NIH3T3)
and PARP-1 knock-out mouse embryonic fibroblasts (PARP1-/-). The lack of PARP-1 increased the abundance of TET2 on the Cxcl12 promoter, suggesting that TET-mediated demethylation provoked by the absence of PARP-1 could account for the observed increased expression of this chemokine. Deciphering the regulation of DNA (de)methylation factors that control Cxcl12 expression may provide an additional therapeutic approach in pharmacological interventions where gene switching on or off based on targeted stimulation or inhibition is necessary.",
journal = "Archives of Biological Sciences",
title = "Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1",
number = "3",
volume = "71",
doi = "10.2298/ABS190404027T",
pages = "455-462"
}

Tolić, A., Rajić, J., Đorđević, M., Đorđević, M., Dinić, S., Grdović, N., Arambašić Jovanović, J., Mihailović, M., Poznanović, G., Jurkowski, T. P., Vidaković, M.,& Uskoković, A.. (2019). Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1. in Archives of Biological Sciences, 71(3), 455-462.
https://doi.org/10.2298/ABS190404027T

Tolić A, Rajić J, Đorđević M, Đorđević M, Dinić S, Grdović N, Arambašić Jovanović J, Mihailović M, Poznanović G, Jurkowski TP, Vidaković M, Uskoković A. Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1. in Archives of Biological Sciences. 2019;71(3):455-462.
doi:10.2298/ABS190404027T .

Tolić, Anja, Rajić, Jovana, Đorđević, Marija, Đorđević, Miloš, Dinić, Svetlana, Grdović, Nevena, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Poznanović, Goran, Jurkowski, Tomasz P., Vidaković, Melita, Uskoković, Aleksandra, "Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1" in Archives of Biological Sciences, 71, no. 3 (2019):455-462,
https://doi.org/10.2298/ABS190404027T . .