Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200027 (Institute of Forestry, Belgrade)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200027 (Institute of Forestry, Belgrade) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200027 (Institut za šumarstvo, Beograd) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200027 (Институт за шумарство, Београд) (sr)
Authors

Publications

Optimization of In Vitro Regeneration of Pinus peuce (Gris.)

Stojičić, Dragana; Budimir, Snežana; Čokeša, Vlado; Uzelac, Branka

(Basel : MDPI, 2024)

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Čokeša, Vlado
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6521
AB  - Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Optimization of In Vitro Regeneration of Pinus peuce (Gris.)
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010097
SP  - 97
ER  - 
@article{
author = "Stojičić, Dragana and Budimir, Snežana and Čokeša, Vlado and Uzelac, Branka",
year = "2024",
abstract = "Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010097",
pages = "97"
}
Stojičić, D., Budimir, S., Čokeša, V.,& Uzelac, B.. (2024). Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae
Basel : MDPI., 10(1), 97.
https://doi.org/10.3390/horticulturae10010097
Stojičić D, Budimir S, Čokeša V, Uzelac B. Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae. 2024;10(1):97.
doi:10.3390/horticulturae10010097 .
Stojičić, Dragana, Budimir, Snežana, Čokeša, Vlado, Uzelac, Branka, "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)" in Horticulturae, 10, no. 1 (2024):97,
https://doi.org/10.3390/horticulturae10010097 . .

Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens

Milanović, Slobodan; Milenković, Ivan; Lazarević, Jelica; Todosijević, Marina; Ljujić, Jovana; Mitić, Zorica; Nikolić, Biljana; Marin, Petar; Tešević, Vele

(Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade, 2024)

TY  - DATA
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Lazarević, Jelica
AU  - Todosijević, Marina
AU  - Ljujić, Jovana
AU  - Mitić, Zorica
AU  - Nikolić, Biljana
AU  - Marin, Petar
AU  - Tešević, Vele
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6490
AB  - We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6490
ER  - 
@misc{
author = "Milanović, Slobodan and Milenković, Ivan and Lazarević, Jelica and Todosijević, Marina and Ljujić, Jovana and Mitić, Zorica and Nikolić, Biljana and Marin, Petar and Tešević, Vele",
year = "2024",
abstract = "We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6490"
}
Milanović, S., Milenković, I., Lazarević, J., Todosijević, M., Ljujić, J., Mitić, Z., Nikolić, B., Marin, P.,& Tešević, V.. (2024). Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6490
Milanović S, Milenković I, Lazarević J, Todosijević M, Ljujić J, Mitić Z, Nikolić B, Marin P, Tešević V. Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .
Milanović, Slobodan, Milenković, Ivan, Lazarević, Jelica, Todosijević, Marina, Ljujić, Jovana, Mitić, Zorica, Nikolić, Biljana, Marin, Petar, Tešević, Vele, "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors

Popović, Vladan; Šešlija Jovanović, Darka; Lučić, Aleksandar; Rakonjac, Ljubinko; Jovanović, Sanja; Vasiljević, Aleksandar; Miljković, Danijela

(Zagreb: Croatian Forestry Society, 2022)

TY  - JOUR
AU  - Popović, Vladan
AU  - Šešlija Jovanović, Darka
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Jovanović, Sanja
AU  - Vasiljević, Aleksandar
AU  - Miljković, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5131
AB  - Interpopulation and intrapopulation variability of three morphological needle traits (length, width and thickness) was investigated in 16 natural silver fir populations in the Balkan Peninsula. The populations represent refugial areas of silver fir (Abies alba Mill.). This paper aims to provide a comprehensive analysis of the influence of climatic factors (mean annual temperature, number of days with temperatures < 0, > 5, < 18, > 18oC, Hargreaves climatic moisture deficit and De Martonne aridity index, on the pattern of morphological needle traits within each population. Populations showed variation in the analyzed morphological needle traits, which could not be clearly defined by any of the analyzed climatic factors. The De Martonne aridity index and Hargreaves climatic moisture deficit had the greatest impact on the trait values, whereas the mean annual precipitation had the lowest. Evolutionary ecology research of the silver fir needle morphology is a valuable contribution to the comprehention of the present genetic variability as a prerequisite for adaptation to the rapid climate change and conservation of the species area in the Balkan Peninsula region.
AB  - Interpopulacijska i intrapopulacijska varijabilnost triju morfoloških osobina iglica (dužina, širina i
debljina) istraživana je u 16 prirodnih populacija jele na Balkanskom poluotoku. Populacije predstav-
ljaju refugijalna područja jele (Abies alba Mill.). Ovaj rad ima za cilj pružiti opsežnu analizu utjecaja
klimatskih čimbenika (srednja godišnja temperatura, broj dana s temperaturama < 0, > 5, < 18, > 18°C,
Hargreavesov klimatski deficit vlage i De Martonneov indeks aridnosti) na obrazac morfoloških
obilježja iglica unutar svake populacije. Populacije su pokazale varijacije u analiziranim morfološkim
obilježjima iglica koje se nisu mogle jasno definirati niti jednim od analiziranih klimatskih čimbenika.
Najveći utjecaj na vrijednosti obilježja imao je klimatski deficit vlage po Hargreavesu, a zatim najniže
vrijednosti srednje godišnje količine oborina. Evolucijska ekološka istraživanja morfologije iglica jele
vrijedan su doprinos razumijevanju sadašnje genetske varijabilnosti kao preduvjeta za prilagodbu na
brze klimatske promjene i očuvanje vrste na području Balkanskog poluotoka.
PB  - Zagreb: Croatian Forestry Society
T2  - Šumski list
T1  - Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors
T1  - Prostorna varijabilnost morfoloških obilježja iglica populacija jele (Abies alba Mill.) na Balkanskom poluotoku u odnosu na klimatske čimbenike
VL  - 7-8
DO  - 10.31298/SL.146.7-8.3
SP  - 309
EP  - 318
ER  - 
@article{
author = "Popović, Vladan and Šešlija Jovanović, Darka and Lučić, Aleksandar and Rakonjac, Ljubinko and Jovanović, Sanja and Vasiljević, Aleksandar and Miljković, Danijela",
year = "2022",
abstract = "Interpopulation and intrapopulation variability of three morphological needle traits (length, width and thickness) was investigated in 16 natural silver fir populations in the Balkan Peninsula. The populations represent refugial areas of silver fir (Abies alba Mill.). This paper aims to provide a comprehensive analysis of the influence of climatic factors (mean annual temperature, number of days with temperatures < 0, > 5, < 18, > 18oC, Hargreaves climatic moisture deficit and De Martonne aridity index, on the pattern of morphological needle traits within each population. Populations showed variation in the analyzed morphological needle traits, which could not be clearly defined by any of the analyzed climatic factors. The De Martonne aridity index and Hargreaves climatic moisture deficit had the greatest impact on the trait values, whereas the mean annual precipitation had the lowest. Evolutionary ecology research of the silver fir needle morphology is a valuable contribution to the comprehention of the present genetic variability as a prerequisite for adaptation to the rapid climate change and conservation of the species area in the Balkan Peninsula region., Interpopulacijska i intrapopulacijska varijabilnost triju morfoloških osobina iglica (dužina, širina i
debljina) istraživana je u 16 prirodnih populacija jele na Balkanskom poluotoku. Populacije predstav-
ljaju refugijalna područja jele (Abies alba Mill.). Ovaj rad ima za cilj pružiti opsežnu analizu utjecaja
klimatskih čimbenika (srednja godišnja temperatura, broj dana s temperaturama < 0, > 5, < 18, > 18°C,
Hargreavesov klimatski deficit vlage i De Martonneov indeks aridnosti) na obrazac morfoloških
obilježja iglica unutar svake populacije. Populacije su pokazale varijacije u analiziranim morfološkim
obilježjima iglica koje se nisu mogle jasno definirati niti jednim od analiziranih klimatskih čimbenika.
Najveći utjecaj na vrijednosti obilježja imao je klimatski deficit vlage po Hargreavesu, a zatim najniže
vrijednosti srednje godišnje količine oborina. Evolucijska ekološka istraživanja morfologije iglica jele
vrijedan su doprinos razumijevanju sadašnje genetske varijabilnosti kao preduvjeta za prilagodbu na
brze klimatske promjene i očuvanje vrste na području Balkanskog poluotoka.",
publisher = "Zagreb: Croatian Forestry Society",
journal = "Šumski list",
title = "Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors, Prostorna varijabilnost morfoloških obilježja iglica populacija jele (Abies alba Mill.) na Balkanskom poluotoku u odnosu na klimatske čimbenike",
volume = "7-8",
doi = "10.31298/SL.146.7-8.3",
pages = "309-318"
}
Popović, V., Šešlija Jovanović, D., Lučić, A., Rakonjac, L., Jovanović, S., Vasiljević, A.,& Miljković, D.. (2022). Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors. in Šumski list
Zagreb: Croatian Forestry Society., 7-8, 309-318.
https://doi.org/10.31298/SL.146.7-8.3
Popović V, Šešlija Jovanović D, Lučić A, Rakonjac L, Jovanović S, Vasiljević A, Miljković D. Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors. in Šumski list. 2022;7-8:309-318.
doi:10.31298/SL.146.7-8.3 .
Popović, Vladan, Šešlija Jovanović, Darka, Lučić, Aleksandar, Rakonjac, Ljubinko, Jovanović, Sanja, Vasiljević, Aleksandar, Miljković, Danijela, "Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors" in Šumski list, 7-8 (2022):309-318,
https://doi.org/10.31298/SL.146.7-8.3 . .
1

Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient

Popović, Vladan; Nikolić, Biljana; Lučić, Aleksandar; Rakonjac, Ljubinko; Šešlija Jovanović, Darka; Miljković, Danijela

(Berlin : Springer International, 2022)

TY  - JOUR
AU  - Popović, Vladan
AU  - Nikolić, Biljana
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Šešlija Jovanović, Darka
AU  - Miljković, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4792
AB  - Geographical and climatic characteristics (elevation, mean annual temperature, summer heat: moisture index, evaporation,
climatic moisture deficit, growing degree-days, heating degree-days, de Martonne’s aridity index) drive variations in
the morphological and anatomical traits of the Norway spruce (Picea abies L. Karst) needle (needle length, needle thickness,
needle width, epidermis thickness with cuticle, hypodermis height, central bundle diameter, resin duct diameter, and the
number of resin ducts). We studied the pattern of variation in these needle characteristics in 15 Balkan Mountains (BM) and
Dinaric Alps (DA) natural populations (altitudes ranging from 1100 to 1860 and from 980 to 1530 m a.s.l., respectively).
The needle traits showed significant differentiation between mountain regions, between populations and within populations.
The largest contribution of genetic component in phenotypic variation had the needle length, both components had the same
share in the needle width, whereas the environmental component of variability had the largest share in the other needle traits.
The principal component analysis revealed that anatomical needle traits and the climatic conditions contributed to differences
between populations from different mountain regions. An agglomerative hierarchical clustering analysis revealed three
dendogram clusters: three BM populations made one cluster, DA populations made the second, whereas seven populations
close to the BM populations and five close to the DA populations made the third cluster. Patterns of morpho-anatomical
phenotypic variability point to selective mechanisms and adjustments, enable to define the boundaries of climate niches,
and may provide a starting point for conservation program according to climate change projections in the Balkan region.
PB  - Berlin : Springer International
T2  - Trees
T1  - Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient
DO  - 10.1007/s00468-022-02277-1
ER  - 
@article{
author = "Popović, Vladan and Nikolić, Biljana and Lučić, Aleksandar and Rakonjac, Ljubinko and Šešlija Jovanović, Darka and Miljković, Danijela",
year = "2022",
abstract = "Geographical and climatic characteristics (elevation, mean annual temperature, summer heat: moisture index, evaporation,
climatic moisture deficit, growing degree-days, heating degree-days, de Martonne’s aridity index) drive variations in
the morphological and anatomical traits of the Norway spruce (Picea abies L. Karst) needle (needle length, needle thickness,
needle width, epidermis thickness with cuticle, hypodermis height, central bundle diameter, resin duct diameter, and the
number of resin ducts). We studied the pattern of variation in these needle characteristics in 15 Balkan Mountains (BM) and
Dinaric Alps (DA) natural populations (altitudes ranging from 1100 to 1860 and from 980 to 1530 m a.s.l., respectively).
The needle traits showed significant differentiation between mountain regions, between populations and within populations.
The largest contribution of genetic component in phenotypic variation had the needle length, both components had the same
share in the needle width, whereas the environmental component of variability had the largest share in the other needle traits.
The principal component analysis revealed that anatomical needle traits and the climatic conditions contributed to differences
between populations from different mountain regions. An agglomerative hierarchical clustering analysis revealed three
dendogram clusters: three BM populations made one cluster, DA populations made the second, whereas seven populations
close to the BM populations and five close to the DA populations made the third cluster. Patterns of morpho-anatomical
phenotypic variability point to selective mechanisms and adjustments, enable to define the boundaries of climate niches,
and may provide a starting point for conservation program according to climate change projections in the Balkan region.",
publisher = "Berlin : Springer International",
journal = "Trees",
title = "Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient",
doi = "10.1007/s00468-022-02277-1"
}
Popović, V., Nikolić, B., Lučić, A., Rakonjac, L., Šešlija Jovanović, D.,& Miljković, D.. (2022). Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient. in Trees
Berlin : Springer International..
https://doi.org/10.1007/s00468-022-02277-1
Popović V, Nikolić B, Lučić A, Rakonjac L, Šešlija Jovanović D, Miljković D. Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient. in Trees. 2022;.
doi:10.1007/s00468-022-02277-1 .
Popović, Vladan, Nikolić, Biljana, Lučić, Aleksandar, Rakonjac, Ljubinko, Šešlija Jovanović, Darka, Miljković, Danijela, "Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient" in Trees (2022),
https://doi.org/10.1007/s00468-022-02277-1 . .
9
9

Headspace volatiles isolated from twigs of Picea omorika from Serbia

Nikolić, Biljana; Ljujić, Jovana; Bojović, Srđan; Mitić, Zorica; Rajčević, Nemanja; Tešević, Vele; Marin, Petar

(Serbian Biological Society, 2020)

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Ljujić, Jovana
AU  - Bojović, Srđan
AU  - Mitić, Zorica
AU  - Rajčević, Nemanja
AU  - Tešević, Vele
AU  - Marin, Petar
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000038N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4040
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5348
AB  - The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Headspace volatiles isolated from twigs of Picea omorika from Serbia
IS  - 3
VL  - 72
DO  - 10.2298/ABS200511038N
SP  - 445
EP  - 452
ER  - 
@article{
author = "Nikolić, Biljana and Ljujić, Jovana and Bojović, Srđan and Mitić, Zorica and Rajčević, Nemanja and Tešević, Vele and Marin, Petar",
year = "2020",
abstract = "The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Headspace volatiles isolated from twigs of Picea omorika from Serbia",
number = "3",
volume = "72",
doi = "10.2298/ABS200511038N",
pages = "445-452"
}
Nikolić, B., Ljujić, J., Bojović, S., Mitić, Z., Rajčević, N., Tešević, V.,& Marin, P.. (2020). Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences
Serbian Biological Society., 72(3), 445-452.
https://doi.org/10.2298/ABS200511038N
Nikolić B, Ljujić J, Bojović S, Mitić Z, Rajčević N, Tešević V, Marin P. Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences. 2020;72(3):445-452.
doi:10.2298/ABS200511038N .
Nikolić, Biljana, Ljujić, Jovana, Bojović, Srđan, Mitić, Zorica, Rajčević, Nemanja, Tešević, Vele, Marin, Petar, "Headspace volatiles isolated from twigs of Picea omorika from Serbia" in Archives of Biological Sciences, 72, no. 3 (2020):445-452,
https://doi.org/10.2298/ABS200511038N . .
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