TY  - JOUR
AU  - Krajnović, Tamara
AU  - Kaluđerović, Goran N
AU  - Wessjohann, Ludger A
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1043661816000189
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3306
AB  - Isoxanthohumol (IXN), a prenylated flavonoid from hops, exhibits diverse biological activities, e.g. antitumor, antiinflammatory, antioxidant and antiangiogenic. In this study, the effect of IXN is evaluated on two melanoma cell lines with dissimilar molecular background, B16 and A375. The treatment of both cell lines with IXN resulted in dose-dependent decrease of cell viability. Abolished viability was in correlation with changed morphology and loss of dividing potential indicating phenotypical alteration of both tested cell lines. While modified B16 cells underwent the process of non-classic differentiation followed by tyrosinase activity without enhancement of melanin content, inhibition of Notch 1, β-catenin and Oct-3/4 was observed in A375 cells indicating loss of their pluripotent characteristics. In parallel with this, distinct subpopulations in both cell cultures entered the process of programmed cell death-apoptosis in a caspase independent manner. The described changes in cultures upon exposure to IXN could be connected with the suppression of reactive oxygen (ROS) and nitrogen species (RNS) induced by the drug. Despite the differences in which IXN promoted modifications in the upper part of the PI3K/Akt and MEK-ERK signaling pathways between B16 and A375 cells, p70S6K and its target S6 protein in both types of melanoma cells, after transient activation, became inhibited. In addition to direct input of IXN on cell viability, this study for the first time shows that IXN strongly sensitizes melanoma cells to the treatment with paclitaxel in vivo, in concordance with data obtained in vitro on B16 cells as well as their highly invasive F10 subclone.
PB  - Academic Press
T2  - Pharmacological Research
T1  - Versatile antitumor potential of isoxanthohumol: Enhancement of paclitaxel activity in vivo
VL  - 105
DO  - 10.1016/j.phrs.2016.01.011
SP  - 62
EP  - 73
ER  - 

@article{
author = "Krajnović, Tamara and Kaluđerović, Goran N and Wessjohann, Ludger A and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2016",
abstract = "Isoxanthohumol (IXN), a prenylated flavonoid from hops, exhibits diverse biological activities, e.g. antitumor, antiinflammatory, antioxidant and antiangiogenic. In this study, the effect of IXN is evaluated on two melanoma cell lines with dissimilar molecular background, B16 and A375. The treatment of both cell lines with IXN resulted in dose-dependent decrease of cell viability. Abolished viability was in correlation with changed morphology and loss of dividing potential indicating phenotypical alteration of both tested cell lines. While modified B16 cells underwent the process of non-classic differentiation followed by tyrosinase activity without enhancement of melanin content, inhibition of Notch 1, β-catenin and Oct-3/4 was observed in A375 cells indicating loss of their pluripotent characteristics. In parallel with this, distinct subpopulations in both cell cultures entered the process of programmed cell death-apoptosis in a caspase independent manner. The described changes in cultures upon exposure to IXN could be connected with the suppression of reactive oxygen (ROS) and nitrogen species (RNS) induced by the drug. Despite the differences in which IXN promoted modifications in the upper part of the PI3K/Akt and MEK-ERK signaling pathways between B16 and A375 cells, p70S6K and its target S6 protein in both types of melanoma cells, after transient activation, became inhibited. In addition to direct input of IXN on cell viability, this study for the first time shows that IXN strongly sensitizes melanoma cells to the treatment with paclitaxel in vivo, in concordance with data obtained in vitro on B16 cells as well as their highly invasive F10 subclone.",
publisher = "Academic Press",
journal = "Pharmacological Research",
title = "Versatile antitumor potential of isoxanthohumol: Enhancement of paclitaxel activity in vivo",
volume = "105",
doi = "10.1016/j.phrs.2016.01.011",
pages = "62-73"
}

Krajnović, T., Kaluđerović, G. N., Wessjohann, L. A., Mijatović, S.,& Maksimović-Ivanić, D.. (2016). Versatile antitumor potential of isoxanthohumol: Enhancement of paclitaxel activity in vivo. in Pharmacological Research
Academic Press., 105, 62-73.
https://doi.org/10.1016/j.phrs.2016.01.011

Krajnović T, Kaluđerović GN, Wessjohann LA, Mijatović S, Maksimović-Ivanić D. Versatile antitumor potential of isoxanthohumol: Enhancement of paclitaxel activity in vivo. in Pharmacological Research. 2016;105:62-73.
doi:10.1016/j.phrs.2016.01.011 .

Krajnović, Tamara, Kaluđerović, Goran N, Wessjohann, Ludger A, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Versatile antitumor potential of isoxanthohumol: Enhancement of paclitaxel activity in vivo" in Pharmacological Research, 105 (2016):62-73,
https://doi.org/10.1016/j.phrs.2016.01.011 . .