TY  - CONF
AU  - Jakubek, Patrycja
AU  - Baranowska, Monika
AU  - Rajić, Jovana
AU  - Vidaković, Melita
AU  - Bartoszek, Agnieszka
AU  - Namiesnik, Jacek
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5806
AB  - Flavonoids, a group of abundant food polyphenols, support the endogenous
antioxidant system and maintenance of redox homeostasis by scavenging reactive
oxygen species. This group of flavonoids includes catechins - antioxidants present in
green tea, known for providing numerous health benefits. Recently green tea catechins
have been reported to affect DNA methylation. Regarding the reversible nature of
epigenetic modifications, deeper insight into relationship between cellular redox state
and DNA methylation may contribute to a better comprehension of the role of
antioxidants in chemoprevention and treatment of chronic diseases
Our previous results have showed that the low (1 μM) and the high (10 μM) doses
of catechins applied to human colon adenocarcinoma cell line (HT29) induce different
changes in the expression of some redox-related genes [1]. Based on this, we aimed at
examining the methylation profiles in the promoter areas of the up-regulated HSPA1A
and the down-regulated SRXN1 using Methylation-Specific PCR and Methylation-
Sensitive High Resolution Melting. The objective of the research was to find out whether
the observed dose dependence was caused by epigenetic modulation of gene expression.
In the case of HSPA1A, methylation level within examined CpG island was not
significantly affected by catechins, regardless of concentration. Conversely, the
methylation level within SRXN1 CpG island was significantly increased when cells were
exposed to the high concentration of catechins.
In conclusion, the methylation levels of CpGs located in the promoter area of
SRXN1 and HSPA1A can be modulated by catechins exhibiting strong antioxidant activity,
therefore the observed modulation seems to be redox-sensitive. Moreover, catechins so
far have been considered as inhibitors of methylation, whereas they can also act as
methylation inducers. This dual function may derive from their ability to influence cellular
redox homeostasis.
PB  - COST Action CA16112
C3  - Personalized Nutrition in aging society: redox control of major age-related diseases: COST Action 16112 NutRedOx; 2018 sep 19-21; Gdańsk, Poland
T1  - Catechins as epigenetic modulators
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5806
ER  - 

@conference{
author = "Jakubek, Patrycja and Baranowska, Monika and Rajić, Jovana and Vidaković, Melita and Bartoszek, Agnieszka and Namiesnik, Jacek",
year = "2018",
abstract = "Flavonoids, a group of abundant food polyphenols, support the endogenous
antioxidant system and maintenance of redox homeostasis by scavenging reactive
oxygen species. This group of flavonoids includes catechins - antioxidants present in
green tea, known for providing numerous health benefits. Recently green tea catechins
have been reported to affect DNA methylation. Regarding the reversible nature of
epigenetic modifications, deeper insight into relationship between cellular redox state
and DNA methylation may contribute to a better comprehension of the role of
antioxidants in chemoprevention and treatment of chronic diseases
Our previous results have showed that the low (1 μM) and the high (10 μM) doses
of catechins applied to human colon adenocarcinoma cell line (HT29) induce different
changes in the expression of some redox-related genes [1]. Based on this, we aimed at
examining the methylation profiles in the promoter areas of the up-regulated HSPA1A
and the down-regulated SRXN1 using Methylation-Specific PCR and Methylation-
Sensitive High Resolution Melting. The objective of the research was to find out whether
the observed dose dependence was caused by epigenetic modulation of gene expression.
In the case of HSPA1A, methylation level within examined CpG island was not
significantly affected by catechins, regardless of concentration. Conversely, the
methylation level within SRXN1 CpG island was significantly increased when cells were
exposed to the high concentration of catechins.
In conclusion, the methylation levels of CpGs located in the promoter area of
SRXN1 and HSPA1A can be modulated by catechins exhibiting strong antioxidant activity,
therefore the observed modulation seems to be redox-sensitive. Moreover, catechins so
far have been considered as inhibitors of methylation, whereas they can also act as
methylation inducers. This dual function may derive from their ability to influence cellular
redox homeostasis.",
publisher = "COST Action CA16112",
journal = "Personalized Nutrition in aging society: redox control of major age-related diseases: COST Action 16112 NutRedOx; 2018 sep 19-21; Gdańsk, Poland",
title = "Catechins as epigenetic modulators",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5806"
}

Jakubek, P., Baranowska, M., Rajić, J., Vidaković, M., Bartoszek, A.,& Namiesnik, J.. (2018). Catechins as epigenetic modulators. in Personalized Nutrition in aging society: redox control of major age-related diseases: COST Action 16112 NutRedOx; 2018 sep 19-21; Gdańsk, Poland
COST Action CA16112., 35.
https://hdl.handle.net/21.15107/rcub_ibiss_5806

Jakubek P, Baranowska M, Rajić J, Vidaković M, Bartoszek A, Namiesnik J. Catechins as epigenetic modulators. in Personalized Nutrition in aging society: redox control of major age-related diseases: COST Action 16112 NutRedOx; 2018 sep 19-21; Gdańsk, Poland. 2018;:35.
https://hdl.handle.net/21.15107/rcub_ibiss_5806 .

Jakubek, Patrycja, Baranowska, Monika, Rajić, Jovana, Vidaković, Melita, Bartoszek, Agnieszka, Namiesnik, Jacek, "Catechins as epigenetic modulators" in Personalized Nutrition in aging society: redox control of major age-related diseases: COST Action 16112 NutRedOx; 2018 sep 19-21; Gdańsk, Poland (2018):35,
https://hdl.handle.net/21.15107/rcub_ibiss_5806 .