TY  - JOUR
AU  - Miljković, Danijela
AU  - Selaković, Sara
AU  - Vujić, Vukica
AU  - Stanisavljević, Nemanja
AU  - Radović, Svetlana
AU  - Cvetković, Dragana
PY  - 2018
UR  - http://link.springer.com/10.1007/s00035-018-0203-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3040
AB  - Light environments can influence variation in plant morphology, development and susceptibility to herbivores. Our research interest was to investigate the patterns of herbivore damage and developmental stability in dioecious understory forb Mercurialis perennis in contrasting light habitats, located at 1700 m a.s.l. on Mt. Kopaonik. Male and female plants from two light habitats, open (a sun-exposed field) and shaded (a spruce forest) were examined with respect to: herbivore damage (percentage of leaf area loss), fluctuating asymetry (FA) as a measurement of developmental stability, plant morphological and, specifically, leaf size traits, as well as biochemical traits relating to nutritional quality and defence, taking into account the possible presence of intersexual differences. Our results show that herbivore damage was significantly higher in open habitat, as well as one out of four univariate FA indices and the multivariate index. Morphological and biochemical traits, apart from defensive compounds, had higher values in the shade, pointing to sun-exposed habitat being more stressful for this species. Intersexual differences were observed for foliar damage, defensive compounds (phenolics and tannins), all leaf size traits, total leaf area, and protein content. Contrasting light habitats affected most of the analysed traits. Both foliar damage and FA were higher in a more stressful habitat; within habitats, no positive correlations were found. Herbivore damage was significantly male biased in open habitat. The analysis of intersexual differences in developmental stability measured by leaf asymmetry levels provided no evidence that female plants were more sensitive to environmental stress.
T2  - Alpine Botany
T1  - Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats
IS  - 2
VL  - 128
DO  - 10.1007/s00035-018-0203-8
SP  - 193
EP  - 206
ER  - 

@article{
author = "Miljković, Danijela and Selaković, Sara and Vujić, Vukica and Stanisavljević, Nemanja and Radović, Svetlana and Cvetković, Dragana",
year = "2018",
abstract = "Light environments can influence variation in plant morphology, development and susceptibility to herbivores. Our research interest was to investigate the patterns of herbivore damage and developmental stability in dioecious understory forb Mercurialis perennis in contrasting light habitats, located at 1700 m a.s.l. on Mt. Kopaonik. Male and female plants from two light habitats, open (a sun-exposed field) and shaded (a spruce forest) were examined with respect to: herbivore damage (percentage of leaf area loss), fluctuating asymetry (FA) as a measurement of developmental stability, plant morphological and, specifically, leaf size traits, as well as biochemical traits relating to nutritional quality and defence, taking into account the possible presence of intersexual differences. Our results show that herbivore damage was significantly higher in open habitat, as well as one out of four univariate FA indices and the multivariate index. Morphological and biochemical traits, apart from defensive compounds, had higher values in the shade, pointing to sun-exposed habitat being more stressful for this species. Intersexual differences were observed for foliar damage, defensive compounds (phenolics and tannins), all leaf size traits, total leaf area, and protein content. Contrasting light habitats affected most of the analysed traits. Both foliar damage and FA were higher in a more stressful habitat; within habitats, no positive correlations were found. Herbivore damage was significantly male biased in open habitat. The analysis of intersexual differences in developmental stability measured by leaf asymmetry levels provided no evidence that female plants were more sensitive to environmental stress.",
journal = "Alpine Botany",
title = "Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats",
number = "2",
volume = "128",
doi = "10.1007/s00035-018-0203-8",
pages = "193-206"
}

Miljković, D., Selaković, S., Vujić, V., Stanisavljević, N., Radović, S.,& Cvetković, D.. (2018). Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats. in Alpine Botany, 128(2), 193-206.
https://doi.org/10.1007/s00035-018-0203-8

Miljković D, Selaković S, Vujić V, Stanisavljević N, Radović S, Cvetković D. Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats. in Alpine Botany. 2018;128(2):193-206.
doi:10.1007/s00035-018-0203-8 .

Miljković, Danijela, Selaković, Sara, Vujić, Vukica, Stanisavljević, Nemanja, Radović, Svetlana, Cvetković, Dragana, "Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats" in Alpine Botany, 128, no. 2 (2018):193-206,
https://doi.org/10.1007/s00035-018-0203-8 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Marin, Marija
AU  - Radović, Svetlana
AU  - Ninković, Slavica
PY  - 2018
UR  - http://link.springer.com/10.1007/s00709-018-1250-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3064
AB  - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
T2  - Protoplasma
T1  - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.
DO  - 10.1007/s00709-018-1250-0
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Savić, Jelena and Ćosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica",
year = "2018",
abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.",
journal = "Protoplasma",
title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.",
doi = "10.1007/s00709-018-1250-0"
}

Banjac, N., Stanišić, M., Savić, J., Ćosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma.
https://doi.org/10.1007/s00709-018-1250-0

Banjac N, Stanišić M, Savić J, Ćosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma. 2018;.
doi:10.1007/s00709-018-1250-0 .

Banjac, Nevena, Stanišić, Mariana, Savić, Jelena, Ćosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate." in Protoplasma (2018),
https://doi.org/10.1007/s00709-018-1250-0 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Senćanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - http://link.springer.com/10.1007/s11738-018-2677-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3057
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
SP  - 101
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Senćanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9",
pages = "101"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Senćanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum, 40(6), 101.
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Senćanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6):101.
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Senćanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018):101,
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617304486
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2832
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
DO  - 10.1016/j.jff.2017.07.060
SP  - 249
EP  - 262
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Gašić, Uroš and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
doi = "10.1016/j.jff.2017.07.060",
pages = "249-262"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Gašić, U., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods, 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtić B, Nikolovski N, Stanisavljević S, Gašić U, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Gašić, Uroš, Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Stanisavljević, Suzana
AU  - Despotović, Jovana
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6050
AB  - Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4(+) T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, NFκB, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.
PB  - American Chemical Society
T2  - Journal of Agricultural and Food Chemistry
T1  - Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells
IS  - 24
VL  - 64
DO  - 10.1021/acs.jafc.6b00951
SP  - 4900
EP  - 4907
ER  - 

@article{
author = "Jevtić, Bojan and Nikolovski, Neda and Stanisavljević, Suzana and Despotović, Jovana and Miljković, Đorđe and Timotijević, Gordana",
year = "2016",
abstract = "Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4(+) T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, NFκB, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.",
publisher = "American Chemical Society",
journal = "Journal of Agricultural and Food Chemistry",
title = "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells",
number = "24",
volume = "64",
doi = "10.1021/acs.jafc.6b00951",
pages = "4900-4907"
}

Jevtić, B., Nikolovski, N., Stanisavljević, S., Despotović, J., Miljković, Đ.,& Timotijević, G.. (2016). Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry
American Chemical Society., 64(24), 4900-4907.
https://doi.org/10.1021/acs.jafc.6b00951

Jevtić B, Nikolovski N, Stanisavljević S, Despotović J, Miljković Đ, Timotijević G. Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry. 2016;64(24):4900-4907.
doi:10.1021/acs.jafc.6b00951 .

Jevtić, Bojan, Nikolovski, Neda, Stanisavljević, Suzana, Despotović, Jovana, Miljković, Đorđe, Timotijević, Gordana, "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells" in Journal of Agricultural and Food Chemistry, 64, no. 24 (2016):4900-4907,
https://doi.org/10.1021/acs.jafc.6b00951 . .

TY  - GEN
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, Jovanka D.
AU  - Jovanović, Z.
AU  - Orbović, V.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1949
AB  - Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation
   with the blue light of plantlets at dawn resulted in moderate PT
   response regarding both attained curvature and long lag phase. The PT
   response was the strongest between 8:00 and 12:00 h. Throughout the
   afternoon and in the evening, bending rate and maximal PT curvature
   declined significantly until 23:00 h. The GT response was fastest and
   strongest for plantlets stimulated early in the morning and late in the
   evening. During the rest of the day, GT competence did not change much
   apart from a minimum at 15:00. In conditions comprising either prolonged
   day or prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in a short-term. Introduction of dark
   period prior to the tropic stimulation at 11:00 h when both PT and GT
   responses were strong resulted in the opposite effect: PT was depressed
   and GT was enhanced. There was a time threshold of 60 min for the
   duration of dark period so the plants can sense interruption in the
   daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.
T2  - Journal of Plant Growth Regulation
T1  - Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)
IS  - 2
VL  - 34
DO  - 10.1007/s00344-015-9507-8
SP  - 440
EP  - 450
ER  - 

@misc{
author = "Vinterhalter, Dragan and Vinterhalter, Branka and Miljuš-Đukić, Jovanka D. and Jovanović, Z. and Orbović, V.",
year = "2015",
abstract = "Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation
   with the blue light of plantlets at dawn resulted in moderate PT
   response regarding both attained curvature and long lag phase. The PT
   response was the strongest between 8:00 and 12:00 h. Throughout the
   afternoon and in the evening, bending rate and maximal PT curvature
   declined significantly until 23:00 h. The GT response was fastest and
   strongest for plantlets stimulated early in the morning and late in the
   evening. During the rest of the day, GT competence did not change much
   apart from a minimum at 15:00. In conditions comprising either prolonged
   day or prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in a short-term. Introduction of dark
   period prior to the tropic stimulation at 11:00 h when both PT and GT
   responses were strong resulted in the opposite effect: PT was depressed
   and GT was enhanced. There was a time threshold of 60 min for the
   duration of dark period so the plants can sense interruption in the
   daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.",
journal = "Journal of Plant Growth Regulation",
title = "Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)",
number = "2",
volume = "34",
doi = "10.1007/s00344-015-9507-8",
pages = "440-450"
}

Vinterhalter, D., Vinterhalter, B., Miljuš-Đukić, J. D., Jovanović, Z.,& Orbović, V.. (2015). Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014). in Journal of Plant Growth Regulation, 34(2), 440-450.
https://doi.org/10.1007/s00344-015-9507-8

Vinterhalter D, Vinterhalter B, Miljuš-Đukić JD, Jovanović Z, Orbović V. Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014). in Journal of Plant Growth Regulation. 2015;34(2):440-450.
doi:10.1007/s00344-015-9507-8 .

Vinterhalter, Dragan, Vinterhalter, Branka, Miljuš-Đukić, Jovanka D., Jovanović, Z., Orbović, V., "Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)" in Journal of Plant Growth Regulation, 34, no. 2 (2015):440-450,
https://doi.org/10.1007/s00344-015-9507-8 . .

TY  - JOUR
AU  - Đorđević, Valentina
AU  - Timotijević, Gordana
AU  - Pruner, Iva
AU  - Radojković, Dragica
AU  - Milovanović, Boško
AU  - Miljković, Đorđe
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/503
AB  - Background: The mutant CCR5Δ32 allele confers resistance to HIV infection. Several hypotheses regarding its origin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was introduced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was determined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 distribution within the continent. Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild­type CCR5 sequence and a product of 231 bp was obtained from the truncated CCR5Δ32 sequence. Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homozygous and 8.52% are heterozygous for CCR5Δ32. Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, considering ethnically related populations.
AB  - Uvod: Nosioci alela CCR5Δ32 su relativno rezistentni na infekciju HIV-om. Postoji nekoliko hipoteza o poreklu i održanju ovog alela u ljudskoj populaciji. Pretpostavlja se da je mutacija Δ32 nastala u populaciji severne ili istočne Evrope i da se potom proširila ka jugu. Iako je učestalost CCR5Δ32 određena u mnogim evropskim populacijama, dodatni podaci su neophodni za formiranje sveobuhvatne slike o distribuciji CCR5Δ32 u Evropi. Zbog toga smo u našoj studiji odredili učestalost CCR5Δ32 u srpskoj populaciji, za koju do ovog rada nisu postojali takvi podaci. Metode: DNK je izolovana iz periferne krvi 352 osobe. U reakciji lančanog umnožavanja korišćeni su prajmeri specifični za gen CCR5. Dobijen je proizvod od 263 bp na osnovu matrice 'wild type', sekvence CCR5 gena, a proizvod od 231 bp na osnovu okrnjene sekvence gena CCR5 (CCR5Δ32). Ukupna učestalost alela CCR5Δ32 u srpskoj populaciji iznosi 4,55%. Rezultati: Od ukupnog broja analiziranih osoba, identifikovano je 8,52% heterozigotnih i 0,57% homozigotnih nosilaca za ovaj alel. Zaključak: Utvrđena učestalost alela CCR5Δ32 u srpskoj populaciji je neočekivano niska, u poređenju sa učestalošću u ostalim slovenskim populacijama.
T2  - Journal of Medical Biochemistry
T1  - Učestalost alela CCR5Δ32 u srpskoj populaciji
T1  - The frequency of allele CCR5Δ32 in a Serbian population
IS  - 4
VL  - 32
DO  - 10.2478/jomb-2013-0030
SP  - 368
EP  - 374
ER  - 

@article{
author = "Đorđević, Valentina and Timotijević, Gordana and Pruner, Iva and Radojković, Dragica and Milovanović, Boško and Miljković, Đorđe",
year = "2013, 2013",
abstract = "Background: The mutant CCR5Δ32 allele confers resistance to HIV infection. Several hypotheses regarding its origin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was introduced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was determined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 distribution within the continent. Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild­type CCR5 sequence and a product of 231 bp was obtained from the truncated CCR5Δ32 sequence. Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homozygous and 8.52% are heterozygous for CCR5Δ32. Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, considering ethnically related populations., Uvod: Nosioci alela CCR5Δ32 su relativno rezistentni na infekciju HIV-om. Postoji nekoliko hipoteza o poreklu i održanju ovog alela u ljudskoj populaciji. Pretpostavlja se da je mutacija Δ32 nastala u populaciji severne ili istočne Evrope i da se potom proširila ka jugu. Iako je učestalost CCR5Δ32 određena u mnogim evropskim populacijama, dodatni podaci su neophodni za formiranje sveobuhvatne slike o distribuciji CCR5Δ32 u Evropi. Zbog toga smo u našoj studiji odredili učestalost CCR5Δ32 u srpskoj populaciji, za koju do ovog rada nisu postojali takvi podaci. Metode: DNK je izolovana iz periferne krvi 352 osobe. U reakciji lančanog umnožavanja korišćeni su prajmeri specifični za gen CCR5. Dobijen je proizvod od 263 bp na osnovu matrice 'wild type', sekvence CCR5 gena, a proizvod od 231 bp na osnovu okrnjene sekvence gena CCR5 (CCR5Δ32). Ukupna učestalost alela CCR5Δ32 u srpskoj populaciji iznosi 4,55%. Rezultati: Od ukupnog broja analiziranih osoba, identifikovano je 8,52% heterozigotnih i 0,57% homozigotnih nosilaca za ovaj alel. Zaključak: Utvrđena učestalost alela CCR5Δ32 u srpskoj populaciji je neočekivano niska, u poređenju sa učestalošću u ostalim slovenskim populacijama.",
journal = "Journal of Medical Biochemistry",
title = "Učestalost alela CCR5Δ32 u srpskoj populaciji, The frequency of allele CCR5Δ32 in a Serbian population",
number = "4",
volume = "32",
doi = "10.2478/jomb-2013-0030",
pages = "368-374"
}

Đorđević, V., Timotijević, G., Pruner, I., Radojković, D., Milovanović, B.,& Miljković, Đ.. (2013). Učestalost alela CCR5Δ32 u srpskoj populaciji. in Journal of Medical Biochemistry, 32(4), 368-374.
https://doi.org/10.2478/jomb-2013-0030

Đorđević V, Timotijević G, Pruner I, Radojković D, Milovanović B, Miljković Đ. Učestalost alela CCR5Δ32 u srpskoj populaciji. in Journal of Medical Biochemistry. 2013;32(4):368-374.
doi:10.2478/jomb-2013-0030 .

Đorđević, Valentina, Timotijević, Gordana, Pruner, Iva, Radojković, Dragica, Milovanović, Boško, Miljković, Đorđe, "Učestalost alela CCR5Δ32 u srpskoj populaciji" in Journal of Medical Biochemistry, 32, no. 4 (2013):368-374,
https://doi.org/10.2478/jomb-2013-0030 . .