@article{
author = "Koprivica, Ivan and Stanisavljević, Suzana and Mićanović, Dragica and Jevtić, Bojan and Stojanović, Ivana D. and Miljković, Đorđe",
year = "2023",
abstract = "Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous
tissues, where they contribute to the homeostatic immune response in a major
way. Also, they have been increasingly appreciated as important modulators of
chronic inflammatory and autoimmune responses, both locally and systemically.
The proper identification of ILC3 is of utmost importance for meaningful studies
on their role in immunity. Flow cytometry is the method of choice for the
detection and characterization of ILC3. However, the analysis of ILC3-related
papers shows inconsistency in ILC3 phenotypic definition, as different inclusion
and exclusion markers are used for their identification. Here, we present these
discrepancies in the phenotypic characterization of human and mouse ILC3s. We
discuss the pros and cons of using various markers for ILC3 identification.
Furthermore, we consider the possibilities for the efficient isolation and
propagation of ILC3 from different organs and tissues for in-vitro and in-vivo
studies. This paper calls upon uniformity in ILC3 definition, isolation, and
propagation for the increased possibility of confluent interpretation of ILC3’s
role in immunity.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Immunology",
title = "ILC3: a case of conflicted identity",
volume = "14",
doi = "10.3389/fimmu.2023.1271699",
pages = "1271699"
}
