TY  - CONF
AU  - Đorđević, Marija
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Đorđević, Miloš
AU  - Tolić, Anja
AU  - Rajić, Jovana
AU  - Hunyadi, Attila
AU  - Vidaković, Melita
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5802
AB  - Objective: 20-hydroxyecdysone (20HE), a steroid hormone that modulates molting response in insects exerts many pharma­cological effects in mammals, most of which appear beneficial. The aim of this study was to investigate whether 20HE is able to reduce the destruction of beta-cells of the islets of Langerhans and ame­liorate hyperglycemia induced changes in liver tissue in strepto­zotocin (STZ)-induced rat model of diabetes. 
Methods: An experimental model of diabetes was induced in rats by the administration of 35 mg/kg STZ intraperitoneally for 4 consecutive days. 20HE was administered orally at a dose of20 mg/ kg body weight for four weeks, starting from the last day of STZ administration. Pancreas tissue sections were analyzed by hema­toxylin and eosin staining and immunohistochemical staining with insulin. Estimation of oxidative damage of DNA and lipids in the liver were detected by comet assay and thiobarbituric acid-re­active substance assay, respectively. Liver sections were analyzed by hematoxylin/eosin and Masson's trichrome staining. 
Results: Diabetic rats treated with the 20HE displayed several improved biochemical parameters in the circulation: reduced hy­perglycemia, lower triglyceride concentration and reduced glycat­ed hemoglobin. The administration of 20HE to diabetic rats also led to positive histological changes of pancreatic islets and increase in the number of insulin-positive cells in the islets which was ac­companied by increased serum insulin level. These results show that 20HE administration to diabetic rats restrained islet destruc­tion and partially restored the number of insulin-positive cells. In addition, treatment of 20HE attenuated diabetes-induced liver damage in rats according to lower level of DNA damage and reduc­tion of oxidative damage oflipids. This result is in accordance with observed improvement of liver architecture in 20HE treated dia­betic rats. Staining of collagen and increase in E-cadherin and de­creases in a-smooth muscle actin revealed that administration of 20HE to diabetic rats attenuated fibrotic process in the liver. 
Conclusions: 20HE administration seems to be beneficial for improving the hyperglycemia by increasing beta-cell mass and preventing diabetic complications in liver by attenuating fibrotic process.
PB  - Krager Publishers
C3  - Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy
T1  - 20-Hydroxyecdysone Protects Pancreatic Islets and Liver in Streptozotocin-lnduced Diabetic Rats
DO  - 10.1159/000490753
SP  - 14
ER  - 

@conference{
author = "Đorđević, Marija and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Uskoković, Aleksandra and Grdović, Nevena and Dinić, Svetlana and Đorđević, Miloš and Tolić, Anja and Rajić, Jovana and Hunyadi, Attila and Vidaković, Melita",
year = "2018",
abstract = "Objective: 20-hydroxyecdysone (20HE), a steroid hormone that modulates molting response in insects exerts many pharma­cological effects in mammals, most of which appear beneficial. The aim of this study was to investigate whether 20HE is able to reduce the destruction of beta-cells of the islets of Langerhans and ame­liorate hyperglycemia induced changes in liver tissue in strepto­zotocin (STZ)-induced rat model of diabetes. 
Methods: An experimental model of diabetes was induced in rats by the administration of 35 mg/kg STZ intraperitoneally for 4 consecutive days. 20HE was administered orally at a dose of20 mg/ kg body weight for four weeks, starting from the last day of STZ administration. Pancreas tissue sections were analyzed by hema­toxylin and eosin staining and immunohistochemical staining with insulin. Estimation of oxidative damage of DNA and lipids in the liver were detected by comet assay and thiobarbituric acid-re­active substance assay, respectively. Liver sections were analyzed by hematoxylin/eosin and Masson's trichrome staining. 
Results: Diabetic rats treated with the 20HE displayed several improved biochemical parameters in the circulation: reduced hy­perglycemia, lower triglyceride concentration and reduced glycat­ed hemoglobin. The administration of 20HE to diabetic rats also led to positive histological changes of pancreatic islets and increase in the number of insulin-positive cells in the islets which was ac­companied by increased serum insulin level. These results show that 20HE administration to diabetic rats restrained islet destruc­tion and partially restored the number of insulin-positive cells. In addition, treatment of 20HE attenuated diabetes-induced liver damage in rats according to lower level of DNA damage and reduc­tion of oxidative damage oflipids. This result is in accordance with observed improvement of liver architecture in 20HE treated dia­betic rats. Staining of collagen and increase in E-cadherin and de­creases in a-smooth muscle actin revealed that administration of 20HE to diabetic rats attenuated fibrotic process in the liver. 
Conclusions: 20HE administration seems to be beneficial for improving the hyperglycemia by increasing beta-cell mass and preventing diabetic complications in liver by attenuating fibrotic process.",
publisher = "Krager Publishers",
journal = "Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy",
title = "20-Hydroxyecdysone Protects Pancreatic Islets and Liver in Streptozotocin-lnduced Diabetic Rats",
doi = "10.1159/000490753",
pages = "14"
}

Đorđević, M., Arambašić Jovanović, J., Mihailović, M., Uskoković, A., Grdović, N., Dinić, S., Đorđević, M., Tolić, A., Rajić, J., Hunyadi, A.,& Vidaković, M.. (2018). 20-Hydroxyecdysone Protects Pancreatic Islets and Liver in Streptozotocin-lnduced Diabetic Rats. in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy
Krager Publishers., 14.
https://doi.org/10.1159/000490753

Đorđević M, Arambašić Jovanović J, Mihailović M, Uskoković A, Grdović N, Dinić S, Đorđević M, Tolić A, Rajić J, Hunyadi A, Vidaković M. 20-Hydroxyecdysone Protects Pancreatic Islets and Liver in Streptozotocin-lnduced Diabetic Rats. in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy. 2018;:14.
doi:10.1159/000490753 .

Đorđević, Marija, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Uskoković, Aleksandra, Grdović, Nevena, Dinić, Svetlana, Đorđević, Miloš, Tolić, Anja, Rajić, Jovana, Hunyadi, Attila, Vidaković, Melita, "20-Hydroxyecdysone Protects Pancreatic Islets and Liver in Streptozotocin-lnduced Diabetic Rats" in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy (2018):14,
https://doi.org/10.1159/000490753 . .