TY  - JOUR
AU  - Trikka, Fotini A.
AU  - Nikolaidis, Alexandros
AU  - Ignea, Codruta
AU  - Tsaballa, Aphrodite
AU  - Tziveleka, Leto-Aikaterini
AU  - Ioannou, Efstathia
AU  - Roussis, Vassilios
AU  - Stea, Eleni A.
AU  - Božić, Dragana
AU  - Argiriou, Anagnostis
AU  - Kanellis, Angelos K.
AU  - Kampranis, Sotirios C.
AU  - Makris, Antonios M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2335
AB  - Background: Salvia diterpenes have been found to have health promoting
   properties. Among them, carnosic acid and carnosol, tanshinones and
   sclareol are well known for their cardiovascular, antitumor,
   antiinflammatory and antioxidant activities. However, many of these
   compounds are not available at a constant supply and developing
   biotechnological methods for their production could provide a
   sustainable alternative. The transcriptome of S. pomifera glandular
   trichomes was analysed aiming to identify genes that could be used in
   the engineering of synthetic microbial systems.
   Results: In the present study, a thorough metabolite analysis of S.
   pomifera leaves led to the isolation and structure elucidation of
   carnosic acid-family metabolites including one new natural product.
   These labdane diterpenes seem to be synthesized through miltiradiene and
   ferruginol. Transcriptomic analysis of the glandular trichomes from the
   S. pomifera leaves revealed two genes likely involved in miltiradiene
   synthesis. Their products were identified and the corresponding enzymes
   were characterized as copalyl diphosphate synthase (SpCDS) and
   miltiradiene synthase (SpMilS). In addition, several CYP-encoding
   transcripts were identified providing a valuable resource for the
   identification of the biosynthetic mechanism responsible for the
   production of carnosic acid-family metabolites in S. pomifera.
   Conclusions: Our work has uncovered the key enzymes involved in
   miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The
   transcriptomic dataset obtained provides a valuable tool for the
   identification of the CYPs involved in the synthesis of carnosic
   acid-family metabolites.
T2  - Bmc Genomics
T1  - Combined metabolome and transcriptome profiling provides new insights
 into diterpene biosynthesis in S. pomifera glandular trichomes
IS  - 935
VL  - 16
DO  - 10.1186/s12864-015-2147-3
ER  - 

@article{
author = "Trikka, Fotini A. and Nikolaidis, Alexandros and Ignea, Codruta and Tsaballa, Aphrodite and Tziveleka, Leto-Aikaterini and Ioannou, Efstathia and Roussis, Vassilios and Stea, Eleni A. and Božić, Dragana and Argiriou, Anagnostis and Kanellis, Angelos K. and Kampranis, Sotirios C. and Makris, Antonios M.",
year = "2015",
abstract = "Background: Salvia diterpenes have been found to have health promoting
   properties. Among them, carnosic acid and carnosol, tanshinones and
   sclareol are well known for their cardiovascular, antitumor,
   antiinflammatory and antioxidant activities. However, many of these
   compounds are not available at a constant supply and developing
   biotechnological methods for their production could provide a
   sustainable alternative. The transcriptome of S. pomifera glandular
   trichomes was analysed aiming to identify genes that could be used in
   the engineering of synthetic microbial systems.
   Results: In the present study, a thorough metabolite analysis of S.
   pomifera leaves led to the isolation and structure elucidation of
   carnosic acid-family metabolites including one new natural product.
   These labdane diterpenes seem to be synthesized through miltiradiene and
   ferruginol. Transcriptomic analysis of the glandular trichomes from the
   S. pomifera leaves revealed two genes likely involved in miltiradiene
   synthesis. Their products were identified and the corresponding enzymes
   were characterized as copalyl diphosphate synthase (SpCDS) and
   miltiradiene synthase (SpMilS). In addition, several CYP-encoding
   transcripts were identified providing a valuable resource for the
   identification of the biosynthetic mechanism responsible for the
   production of carnosic acid-family metabolites in S. pomifera.
   Conclusions: Our work has uncovered the key enzymes involved in
   miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The
   transcriptomic dataset obtained provides a valuable tool for the
   identification of the CYPs involved in the synthesis of carnosic
   acid-family metabolites.",
journal = "Bmc Genomics",
title = "Combined metabolome and transcriptome profiling provides new insights
 into diterpene biosynthesis in S. pomifera glandular trichomes",
number = "935",
volume = "16",
doi = "10.1186/s12864-015-2147-3"
}

Trikka, F. A., Nikolaidis, A., Ignea, C., Tsaballa, A., Tziveleka, L., Ioannou, E., Roussis, V., Stea, E. A., Božić, D., Argiriou, A., Kanellis, A. K., Kampranis, S. C.,& Makris, A. M.. (2015). Combined metabolome and transcriptome profiling provides new insights
 into diterpene biosynthesis in S. pomifera glandular trichomes. in Bmc Genomics, 16(935).
https://doi.org/10.1186/s12864-015-2147-3

Trikka FA, Nikolaidis A, Ignea C, Tsaballa A, Tziveleka L, Ioannou E, Roussis V, Stea EA, Božić D, Argiriou A, Kanellis AK, Kampranis SC, Makris AM. Combined metabolome and transcriptome profiling provides new insights
 into diterpene biosynthesis in S. pomifera glandular trichomes. in Bmc Genomics. 2015;16(935).
doi:10.1186/s12864-015-2147-3 .

Trikka, Fotini A., Nikolaidis, Alexandros, Ignea, Codruta, Tsaballa, Aphrodite, Tziveleka, Leto-Aikaterini, Ioannou, Efstathia, Roussis, Vassilios, Stea, Eleni A., Božić, Dragana, Argiriou, Anagnostis, Kanellis, Angelos K., Kampranis, Sotirios C., Makris, Antonios M., "Combined metabolome and transcriptome profiling provides new insights
 into diterpene biosynthesis in S. pomifera glandular trichomes" in Bmc Genomics, 16, no. 935 (2015),
https://doi.org/10.1186/s12864-015-2147-3 . .