TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Belić, Maja
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4327
AB  - Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4327
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Devrnja, Nina and Belić, Maja and Milojević, Jelena",
year = "2018",
abstract = "Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4327"
}

Zdravković-Korać, S., Ćalić, D., Devrnja, N., Belić, M.,& Milojević, J.. (2018). Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327

Zdravković-Korać S, Ćalić D, Devrnja N, Belić M, Milojević J. Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Devrnja, Nina, Belić, Maja, Milojević, Jelena, "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):38,
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .

TY  - CONF
AU  - Belić, Maja
AU  - Anačkov, Goran
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Igić, Ružica
AU  - Milojević, Jelena
AU  - Zdravković-Korać, Snežana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4325
AB  - A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4325
ER  - 

@conference{
author = "Belić, Maja and Anačkov, Goran and Ćalić, Dušica and Devrnja, Nina and Igić, Ružica and Milojević, Jelena and Zdravković-Korać, Snežana",
year = "2018",
abstract = "A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4325"
}

Belić, M., Anačkov, G., Ćalić, D., Devrnja, N., Igić, R., Milojević, J.,& Zdravković-Korać, S.. (2018). Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325

Belić M, Anačkov G, Ćalić D, Devrnja N, Igić R, Milojević J, Zdravković-Korać S. Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .

Belić, Maja, Anačkov, Goran, Ćalić, Dušica, Devrnja, Nina, Igić, Ružica, Milojević, Jelena, Zdravković-Korać, Snežana, "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit." in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):30,
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .

TY  - CONF
AU  - Belić, Maja
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4324
AB  - Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections
SP  - 29
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4324
ER  - 

@conference{
author = "Belić, Maja and Devrnja, Nina and Ćalić, Dušica and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2018",
abstract = "Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections",
pages = "29-30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4324"
}

Belić, M., Devrnja, N., Ćalić, D., Zdravković-Korać, S.,& Milojević, J.. (2018). Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 29-30.
https://hdl.handle.net/21.15107/rcub_ibiss_4324

Belić M, Devrnja N, Ćalić D, Zdravković-Korać S, Milojević J. Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:29-30.
https://hdl.handle.net/21.15107/rcub_ibiss_4324 .

Belić, Maja, Devrnja, Nina, Ćalić, Dušica, Zdravković-Korać, Snežana, Milojević, Jelena, "Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):29-30,
https://hdl.handle.net/21.15107/rcub_ibiss_4324 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4322
AB  - The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Potassium iodide promotes bud regeneration from the apical root sections of shallot plants
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4322
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Belić, Maja and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena",
year = "2018",
abstract = "The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Potassium iodide promotes bud regeneration from the apical root sections of shallot plants",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4322"
}

Zdravković-Korać, S., Belić, M., Devrnja, N., Ćalić, D.,& Milojević, J.. (2018). Potassium iodide promotes bud regeneration from the apical root sections of shallot plants. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_4322

Zdravković-Korać S, Belić M, Devrnja N, Ćalić D, Milojević J. Potassium iodide promotes bud regeneration from the apical root sections of shallot plants. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_4322 .

Zdravković-Korać, Snežana, Belić, Maja, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, "Potassium iodide promotes bud regeneration from the apical root sections of shallot plants" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):39,
https://hdl.handle.net/21.15107/rcub_ibiss_4322 .