TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6463
AB  - Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.
PB  - Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
T1  - Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6463
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Petrović, Jovana and Stojković, Dejan and Gašić, Uroš and Milinčić, Danijel and Pešić, Mirjana",
year = "2023",
abstract = "Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.",
publisher = "Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia",
title = "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6463"
}

Ćirić, A., Ivanov, M., Petrović, J., Stojković, D., Gašić, U., Milinčić, D.,& Pešić, M.. (2023). Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463

Ćirić A, Ivanov M, Petrović J, Stojković D, Gašić U, Milinčić D, Pešić M. Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia. 2023;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

Ćirić, Ana, Ivanov, Marija, Petrović, Jovana, Stojković, Dejan, Gašić, Uroš, Milinčić, Danijel, Pešić, Mirjana, "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications" in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia (2023):41,
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .