TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6129
AB  - Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.
PB  - Olomouc: Europian Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration
SP  - 131
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6129
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2019",
abstract = "Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.",
publisher = "Olomouc: Europian Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6129"
}

Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2019). Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
Olomouc: Europian Federation of Biotechnology., 131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129

Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):131-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .