@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2021",
abstract = "Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.",
publisher = "National Institute for Lasers, Plasma and Radiation Physics, Alexandru Ioan Cuza University, Romanian Physical Society",
journal = "19th International conference on plasma physics and applications, CPPA 2021",
title = "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4414"
}
