@conference{
author = "Nikolić, Biljana and Marinković, Jelena and Marković, Tatjana and Radulović, Milena and Mitić Ćulafić, Dragana and Đukanović, Stefana and Ćirić, Ana and Tasić, Gvozden and Bošković, Marko and Marković, Dejan",
year = "2021",
abstract = "The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
,
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30%
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for
different dental applications.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4376"
}
