TY  - JOUR
AU  - Egerić, Marija
AU  - Smičiklas, Ivana
AU  - Dojčinović, Biljana
AU  - Sikirić, Biljana
AU  - Jović, Mihajlo
AU  - Šljivić-Ivanović, Marija
AU  - Čakmak, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0016706119306172?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3391
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3403
AB  - Liming is a common practice in the treatment of acidic and metal contaminated soils, aiming at pH regulation, enhancing of the nutrient availability and attenuation of trace metals mobility. Replacement of natural limestone with alternative soil alkalizers found among waste materials represents a step towards sustainable resource management and reduced waste storage. In this study, waste seashells (SW) and red mud (RM) were applied in different doses to the soil sampled in the vicinity of mining and smelting complex. The soil was characterized by acidic reaction (pH 4.93), increased Cu concentration (219.2 mg/kg) and a very low level of P-supply (3.61 mg P2O5/100 g). The study aimed to quantify and compare additive-induced effects onto soil physicochemical properties, the status of macronutrients and distribution of trace metals. Targeted effect on soil pH was achieved with SW dose of 0.3% and RM dose of 2%. RM was found to be a source of available P and gave rise to the available P concentrations in the soil. Medium level of P-supply (15.60 mg P2O5/100 g) was achieved with RM dose of 5%, however, the increase in soil salinity and total trace elements concentrations have become significant adverse effects at such dose. The decrease in the ion-exchangeable content of Cu and other trace metals was in correlation with the increase in soil pH after the treatments. Redistribution of metal cations was mainly directed to carbonate/acid soluble and Fe, Mn-oxide bonded fraction after SW addition. Even though trace metals concentration has increased in the soil after application of the RM, they were principally found in the residual fraction. The results emphasize low amounts of contained trace elements and lower doses for achieving targeted effects on pH and metal mobility as the main benefits of SW treatments. On the other hand, moderate and controlled use of RM may represent multiple benefits in terms of simultaneous pH regulation, P-supply, and reduced trace metals mobility.
T2  - Geoderma
T1  - Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives
DO  - 10.1016/J.GEODERMA.2019.06.015
ER  - 

@article{
author = "Egerić, Marija and Smičiklas, Ivana and Dojčinović, Biljana and Sikirić, Biljana and Jović, Mihajlo and Šljivić-Ivanović, Marija and Čakmak, Dragan",
year = "2019",
abstract = "Liming is a common practice in the treatment of acidic and metal contaminated soils, aiming at pH regulation, enhancing of the nutrient availability and attenuation of trace metals mobility. Replacement of natural limestone with alternative soil alkalizers found among waste materials represents a step towards sustainable resource management and reduced waste storage. In this study, waste seashells (SW) and red mud (RM) were applied in different doses to the soil sampled in the vicinity of mining and smelting complex. The soil was characterized by acidic reaction (pH 4.93), increased Cu concentration (219.2 mg/kg) and a very low level of P-supply (3.61 mg P2O5/100 g). The study aimed to quantify and compare additive-induced effects onto soil physicochemical properties, the status of macronutrients and distribution of trace metals. Targeted effect on soil pH was achieved with SW dose of 0.3% and RM dose of 2%. RM was found to be a source of available P and gave rise to the available P concentrations in the soil. Medium level of P-supply (15.60 mg P2O5/100 g) was achieved with RM dose of 5%, however, the increase in soil salinity and total trace elements concentrations have become significant adverse effects at such dose. The decrease in the ion-exchangeable content of Cu and other trace metals was in correlation with the increase in soil pH after the treatments. Redistribution of metal cations was mainly directed to carbonate/acid soluble and Fe, Mn-oxide bonded fraction after SW addition. Even though trace metals concentration has increased in the soil after application of the RM, they were principally found in the residual fraction. The results emphasize low amounts of contained trace elements and lower doses for achieving targeted effects on pH and metal mobility as the main benefits of SW treatments. On the other hand, moderate and controlled use of RM may represent multiple benefits in terms of simultaneous pH regulation, P-supply, and reduced trace metals mobility.",
journal = "Geoderma",
title = "Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives",
doi = "10.1016/J.GEODERMA.2019.06.015"
}

Egerić, M., Smičiklas, I., Dojčinović, B., Sikirić, B., Jović, M., Šljivić-Ivanović, M.,& Čakmak, D.. (2019). Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives. in Geoderma.
https://doi.org/10.1016/J.GEODERMA.2019.06.015

Egerić M, Smičiklas I, Dojčinović B, Sikirić B, Jović M, Šljivić-Ivanović M, Čakmak D. Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives. in Geoderma. 2019;.
doi:10.1016/J.GEODERMA.2019.06.015 .

Egerić, Marija, Smičiklas, Ivana, Dojčinović, Biljana, Sikirić, Biljana, Jović, Mihajlo, Šljivić-Ivanović, Marija, Čakmak, Dragan, "Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives" in Geoderma (2019),
https://doi.org/10.1016/J.GEODERMA.2019.06.015 . .

TY  - JOUR
AU  - Egerić, Marija
AU  - Smičiklas, Ivana
AU  - Dojčinović, Biljana
AU  - Sikirić, Biljana
AU  - Jović, Mihajlo
AU  - Šljivić-Ivanović, Marija
AU  - Čakmak, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0016706119306172?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3391
AB  - Liming is a common practice in the treatment of acidic and metal contaminated soils, aiming at pH regulation, enhancing of the nutrient availability and attenuation of trace metals mobility. Replacement of natural limestone with alternative soil alkalizers found among waste materials represents a step towards sustainable resource management and reduced waste storage. In this study, waste seashells (SW) and red mud (RM) were applied in different doses to the soil sampled in the vicinity of mining and smelting complex. The soil was characterized by acidic reaction (pH 4.93), increased Cu concentration (219.2 mg/kg) and a very low level of P-supply (3.61 mg P2O5/100 g). The study aimed to quantify and compare additive-induced effects onto soil physicochemical properties, the status of macronutrients and distribution of trace metals. Targeted effect on soil pH was achieved with SW dose of 0.3% and RM dose of 2%. RM was found to be a source of available P and gave rise to the available P concentrations in the soil. Medium level of P-supply (15.60 mg P2O5/100 g) was achieved with RM dose of 5%, however, the increase in soil salinity and total trace elements concentrations have become significant adverse effects at such dose. The decrease in the ion-exchangeable content of Cu and other trace metals was in correlation with the increase in soil pH after the treatments. Redistribution of metal cations was mainly directed to carbonate/acid soluble and Fe, Mn-oxide bonded fraction after SW addition. Even though trace metals concentration has increased in the soil after application of the RM, they were principally found in the residual fraction. The results emphasize low amounts of contained trace elements and lower doses for achieving targeted effects on pH and metal mobility as the main benefits of SW treatments. On the other hand, moderate and controlled use of RM may represent multiple benefits in terms of simultaneous pH regulation, P-supply, and reduced trace metals mobility.
T2  - Geoderma
T1  - Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives
VL  - 352
DO  - 10.1016/J.GEODERMA.2019.06.015
SP  - 241
EP  - 250
ER  - 

@article{
author = "Egerić, Marija and Smičiklas, Ivana and Dojčinović, Biljana and Sikirić, Biljana and Jović, Mihajlo and Šljivić-Ivanović, Marija and Čakmak, Dragan",
year = "2019",
abstract = "Liming is a common practice in the treatment of acidic and metal contaminated soils, aiming at pH regulation, enhancing of the nutrient availability and attenuation of trace metals mobility. Replacement of natural limestone with alternative soil alkalizers found among waste materials represents a step towards sustainable resource management and reduced waste storage. In this study, waste seashells (SW) and red mud (RM) were applied in different doses to the soil sampled in the vicinity of mining and smelting complex. The soil was characterized by acidic reaction (pH 4.93), increased Cu concentration (219.2 mg/kg) and a very low level of P-supply (3.61 mg P2O5/100 g). The study aimed to quantify and compare additive-induced effects onto soil physicochemical properties, the status of macronutrients and distribution of trace metals. Targeted effect on soil pH was achieved with SW dose of 0.3% and RM dose of 2%. RM was found to be a source of available P and gave rise to the available P concentrations in the soil. Medium level of P-supply (15.60 mg P2O5/100 g) was achieved with RM dose of 5%, however, the increase in soil salinity and total trace elements concentrations have become significant adverse effects at such dose. The decrease in the ion-exchangeable content of Cu and other trace metals was in correlation with the increase in soil pH after the treatments. Redistribution of metal cations was mainly directed to carbonate/acid soluble and Fe, Mn-oxide bonded fraction after SW addition. Even though trace metals concentration has increased in the soil after application of the RM, they were principally found in the residual fraction. The results emphasize low amounts of contained trace elements and lower doses for achieving targeted effects on pH and metal mobility as the main benefits of SW treatments. On the other hand, moderate and controlled use of RM may represent multiple benefits in terms of simultaneous pH regulation, P-supply, and reduced trace metals mobility.",
journal = "Geoderma",
title = "Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives",
volume = "352",
doi = "10.1016/J.GEODERMA.2019.06.015",
pages = "241-250"
}

Egerić, M., Smičiklas, I., Dojčinović, B., Sikirić, B., Jović, M., Šljivić-Ivanović, M.,& Čakmak, D.. (2019). Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives. in Geoderma, 352, 241-250.
https://doi.org/10.1016/J.GEODERMA.2019.06.015

Egerić M, Smičiklas I, Dojčinović B, Sikirić B, Jović M, Šljivić-Ivanović M, Čakmak D. Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives. in Geoderma. 2019;352:241-250.
doi:10.1016/J.GEODERMA.2019.06.015 .

Egerić, Marija, Smičiklas, Ivana, Dojčinović, Biljana, Sikirić, Biljana, Jović, Mihajlo, Šljivić-Ivanović, Marija, Čakmak, Dragan, "Interactions of acidic soil near copper mining and smelting complex and waste-derived alkaline additives" in Geoderma, 352 (2019):241-250,
https://doi.org/10.1016/J.GEODERMA.2019.06.015 . .

TY  - THES
AU  - Milinković, Vedrana
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=1087
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:7792/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024573874
UR  - http://nardus.mpn.gov.rs/123456789/2160
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2396
AB  - Glioblastomi (GBM) predstavljaju najčešći i najmaligniji tip tumora mozga. Iako imaju istu
histopatološku sliku, primarni i sekundarni glioblastomi se razlikuju po mehanizmu nastanka
i setu karakterističnih genetičkih promena. Iako je tokom poslednjih godina došlo do
određenog poboljšanja u sagledavanju njihove kompleksne prirode, glioblastomi i dalje
predstavljaju neizlečivu bolest. Osnovni cilj ove doktorske disertacije bio je detekcija i
kvantifikacija genomske nestabilnosti, kao i identifikacija specifičnih genetičkih promena
odgovornih za promociju i progresiju malignih glioma, a sa dugoročnim ciljem da se definišu
potencijalni molekularni markeri za dijagnozu i prognozu bolesti. Sa tim u vezi, izvršena je i
analiza najčešćih poznatih genetičkih promena u gliomima: detekcija inaktivacije ključnih
tumor supresor gena (p53, PTEN i p16) i amplifikacije EGFR onkogena. Promene dva ili više
navedenih gena su prisutne u većini analiziranih uzoraka, što potvrđuje značaj višestrukih
genskih alteracija u patogenezi glioma.
Upoređivanjem AP-PCR DNK profila tumorskog i zdravog tkiva pacijenata uočena su dva
tipa razlika: kvalitativne razlike koje nastaju usled promena u sekvenci DNK molekula i
predstavljaju manifestaciju mikrosatelitske nestabilnosti i nestabilnosti pojedinačnih
nukleotida (MIN-PIN), kao i kvantitativne razlike nastale usled amplifikacija ili delecija
većih hromozomskih regiona, pokazatelj hromozomske nestabilnosti (CIN). Oba tipa
promena su prisutna u svim analiziranim uzorcima, doprinoseći podjednako visokom stepenu
ukupne genomske nestabilnosti u oba histološka podtipa GBM. Detaljnija analiza DNK
profila je omogućila identifikaciju specifičnih promena 11 novih gena, koji do sada nisu
povezani sa progresijom glioma: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2,
KCNG2, PDE4D, KIR3DL3 i INPP5A. Većina identifikovanih gena ima značajnu ulogu u
procesima signalne transdukcije i ćelijske adhezije, koji su veoma značajni za nastanak i
progresiju kancera. Prisustvo promena u identifikovanim genima je, dalje, korelisano sa
kliničko-patološkim parametrima, stepenom genomske nestabilnosti i preživljavanjem
pacijenata, kao i sa statusom p53, PTEN, p16 i EGFR gena. Pokazane su statistički značajne
ko-alteracije nekih od identifikovanih gena sa p53, p16 i EGFR genima, ali nije pokazana
statistički značajna povezanost između inaktiviranog PTEN-a i bilo kojeg novog
identifikovanog gena. Svi rezultati ove studije potvrđuju da se u osnovi glioma nalaze
izuzetno složeni genetički mehanizmi, pri čemu je naročito značajan visok stepen genomske
nestabilnosti koji potencijalno predstavlja jednu od osnovnih karakteristika ovog tipa
tumora. Osim toga, ova studiija je prva koja je ukazala na potencijalni značaj
identifikovanih gena u patogenezi glioma, kao i na njihovu primenu kao potencijalnih
biomarkera za dijagnostiku i prognozu pacijenata sa primarnim ili sekundarnim
glioblastomima.
AB  - Glioblastoma is the most frequent and the most malignant human brain tumor. Despite a similar
histological appearance, primary and secondary glioblastomas are distinct tumor entities with
different genetic alterations but none being specific enough to distinguish them. Despite better
insight in its complex genetic nature, glioblastoma is still incurable disease, with extremely
short median survival. The purpose of this study was to detect specific genetic changes, as well
as to quantify overall level of genomic instability in samples of malignant glioma patients.
Besides, we analyzed genetic alterations of key tumor suppressors (p53, PTEN and p16) and
EGFR oncogene, commonly aberrant in glioma samples. Alterations of two or more genes were
present in majority of analyzed samples indicating importance of multiple changes for
gliomagenesis.
AP-PCR DNA profiling revealed two types of genetic differences between tumor and normal
tissue: qualitative changes which represent accumulation of changes in DNA sequence and are
the manifestation of microsatellite and point mutation instability (MIN-PIN) and quantitative
changes which represent amplifications or deletions of existing chromosomal material and are
the manifestation of chromosomal instability (CIN). Both types of alterations were present in
all analyzed samples contributing almost equally to the total level of genomic instability, and
showing no differences between histological subtypes. Further investigation of alterations in
DNA profiles revealed specific changes in the following 11 genes that were not previously
associated with glioma pathogenesis: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2,
KCNG2, PDE4D, KIR3DL3, and INPP5A. Most of identified genes have significant role in
signal transduction or cell adhesion, which are important processes for cancer development and
progression.
The frequencies of observed alterations were correlated with clinicopathologic parameters, the
level of genomic instability and patient survival, as well as with presence of alterations in p53,
PTEN, p16 and EGFR genes. Some of the identified genes showed significant association with
p53 and p16 tumor suppressors, as well as with EGFR, but there was no significant correlation
between loss of PTEN and any of identified genes.
In conclusion, our results confirmed complexity of glioma genetic nature, emphasizing high
level of genomic instability as hallmark of this tumor type. Identified novel genes could be used
as potential biomarkers for diagnosis of primary and secondary glioblastoma, as well as
predictors of patients’ outcome.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Detekcija i karakterizacija genomske nestabilnosti tumora mozga glijalnog porekla
T1  - Detection and characterization of genomic instability in patients with malignant glioma
SP  - 1
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2160
ER  - 

@phdthesis{
author = "Milinković, Vedrana",
year = "2013",
abstract = "Glioblastomi (GBM) predstavljaju najčešći i najmaligniji tip tumora mozga. Iako imaju istu
histopatološku sliku, primarni i sekundarni glioblastomi se razlikuju po mehanizmu nastanka
i setu karakterističnih genetičkih promena. Iako je tokom poslednjih godina došlo do
određenog poboljšanja u sagledavanju njihove kompleksne prirode, glioblastomi i dalje
predstavljaju neizlečivu bolest. Osnovni cilj ove doktorske disertacije bio je detekcija i
kvantifikacija genomske nestabilnosti, kao i identifikacija specifičnih genetičkih promena
odgovornih za promociju i progresiju malignih glioma, a sa dugoročnim ciljem da se definišu
potencijalni molekularni markeri za dijagnozu i prognozu bolesti. Sa tim u vezi, izvršena je i
analiza najčešćih poznatih genetičkih promena u gliomima: detekcija inaktivacije ključnih
tumor supresor gena (p53, PTEN i p16) i amplifikacije EGFR onkogena. Promene dva ili više
navedenih gena su prisutne u većini analiziranih uzoraka, što potvrđuje značaj višestrukih
genskih alteracija u patogenezi glioma.
Upoređivanjem AP-PCR DNK profila tumorskog i zdravog tkiva pacijenata uočena su dva
tipa razlika: kvalitativne razlike koje nastaju usled promena u sekvenci DNK molekula i
predstavljaju manifestaciju mikrosatelitske nestabilnosti i nestabilnosti pojedinačnih
nukleotida (MIN-PIN), kao i kvantitativne razlike nastale usled amplifikacija ili delecija
većih hromozomskih regiona, pokazatelj hromozomske nestabilnosti (CIN). Oba tipa
promena su prisutna u svim analiziranim uzorcima, doprinoseći podjednako visokom stepenu
ukupne genomske nestabilnosti u oba histološka podtipa GBM. Detaljnija analiza DNK
profila je omogućila identifikaciju specifičnih promena 11 novih gena, koji do sada nisu
povezani sa progresijom glioma: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2,
KCNG2, PDE4D, KIR3DL3 i INPP5A. Većina identifikovanih gena ima značajnu ulogu u
procesima signalne transdukcije i ćelijske adhezije, koji su veoma značajni za nastanak i
progresiju kancera. Prisustvo promena u identifikovanim genima je, dalje, korelisano sa
kliničko-patološkim parametrima, stepenom genomske nestabilnosti i preživljavanjem
pacijenata, kao i sa statusom p53, PTEN, p16 i EGFR gena. Pokazane su statistički značajne
ko-alteracije nekih od identifikovanih gena sa p53, p16 i EGFR genima, ali nije pokazana
statistički značajna povezanost između inaktiviranog PTEN-a i bilo kojeg novog
identifikovanog gena. Svi rezultati ove studije potvrđuju da se u osnovi glioma nalaze
izuzetno složeni genetički mehanizmi, pri čemu je naročito značajan visok stepen genomske
nestabilnosti koji potencijalno predstavlja jednu od osnovnih karakteristika ovog tipa
tumora. Osim toga, ova studiija je prva koja je ukazala na potencijalni značaj
identifikovanih gena u patogenezi glioma, kao i na njihovu primenu kao potencijalnih
biomarkera za dijagnostiku i prognozu pacijenata sa primarnim ili sekundarnim
glioblastomima., Glioblastoma is the most frequent and the most malignant human brain tumor. Despite a similar
histological appearance, primary and secondary glioblastomas are distinct tumor entities with
different genetic alterations but none being specific enough to distinguish them. Despite better
insight in its complex genetic nature, glioblastoma is still incurable disease, with extremely
short median survival. The purpose of this study was to detect specific genetic changes, as well
as to quantify overall level of genomic instability in samples of malignant glioma patients.
Besides, we analyzed genetic alterations of key tumor suppressors (p53, PTEN and p16) and
EGFR oncogene, commonly aberrant in glioma samples. Alterations of two or more genes were
present in majority of analyzed samples indicating importance of multiple changes for
gliomagenesis.
AP-PCR DNA profiling revealed two types of genetic differences between tumor and normal
tissue: qualitative changes which represent accumulation of changes in DNA sequence and are
the manifestation of microsatellite and point mutation instability (MIN-PIN) and quantitative
changes which represent amplifications or deletions of existing chromosomal material and are
the manifestation of chromosomal instability (CIN). Both types of alterations were present in
all analyzed samples contributing almost equally to the total level of genomic instability, and
showing no differences between histological subtypes. Further investigation of alterations in
DNA profiles revealed specific changes in the following 11 genes that were not previously
associated with glioma pathogenesis: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2,
KCNG2, PDE4D, KIR3DL3, and INPP5A. Most of identified genes have significant role in
signal transduction or cell adhesion, which are important processes for cancer development and
progression.
The frequencies of observed alterations were correlated with clinicopathologic parameters, the
level of genomic instability and patient survival, as well as with presence of alterations in p53,
PTEN, p16 and EGFR genes. Some of the identified genes showed significant association with
p53 and p16 tumor suppressors, as well as with EGFR, but there was no significant correlation
between loss of PTEN and any of identified genes.
In conclusion, our results confirmed complexity of glioma genetic nature, emphasizing high
level of genomic instability as hallmark of this tumor type. Identified novel genes could be used
as potential biomarkers for diagnosis of primary and secondary glioblastoma, as well as
predictors of patients’ outcome.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Detekcija i karakterizacija genomske nestabilnosti tumora mozga glijalnog porekla, Detection and characterization of genomic instability in patients with malignant glioma",
pages = "1-140",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2160"
}

Milinković, V.. (2013). Detekcija i karakterizacija genomske nestabilnosti tumora mozga glijalnog porekla. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-140.
https://hdl.handle.net/21.15107/rcub_nardus_2160

Milinković V. Detekcija i karakterizacija genomske nestabilnosti tumora mozga glijalnog porekla. in University of Belgrade, Faculty of Biology. 2013;:1-140.
https://hdl.handle.net/21.15107/rcub_nardus_2160 .

Milinković, Vedrana, "Detekcija i karakterizacija genomske nestabilnosti tumora mozga glijalnog porekla" in University of Belgrade, Faculty of Biology (2013):1-140,
https://hdl.handle.net/21.15107/rcub_nardus_2160 .