TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s42991-020-00019-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3660
AB  - The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.
PB  - Springer
T2  - Mammalian Biology
T1  - 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)
VL  - 100
DO  - 10.1007/s42991-020-00019-9
SP  - 315
EP  - 324
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2020",
abstract = "The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.",
publisher = "Springer",
journal = "Mammalian Biology",
title = "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)",
volume = "100",
doi = "10.1007/s42991-020-00019-9",
pages = "315-324"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2020). 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology
Springer., 100, 315-324.
https://doi.org/10.1007/s42991-020-00019-9

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology. 2020;100:315-324.
doi:10.1007/s42991-020-00019-9 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)" in Mammalian Biology, 100 (2020):315-324,
https://doi.org/10.1007/s42991-020-00019-9 . .

TY  - JOUR
AU  - Klun, Ivana
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Vasilev, Dragan
AU  - Teodorović, Vlado
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - https://www.akademiai.com/doi/10.1556/004.2019.004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3333
AB  - Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6.2%, 95% CI = 4.0-8.4) animals, including 14 red foxes (4.7%), 7 wild cats (35%), 5 beech martens (4.8%), 2 pine martens (16.7%), and 1 European badger (6.25%). No Trichinella larvae were detected in the examined specimens of European polecats, steppe polecats and European otters. Species identification of the Trichinella larvae performed for 18 positive samples revealed T. spiralis in 77.8% and T. britovi in 22.2% of the isolates. Both species were detected in red foxes and wild cats. The predominance of T. spiralis in wildlife in Serbia indicates the (past or present) spillover of this pathogen from domestic to wild animals.
T2  - Acta Veterinaria Hungarica
T2  - Acta Veterinaria Hungarica
T1  - Trichinella spp. in wild mesocarnivores in an endemic setting.
IS  - 1
VL  - 67
DO  - 10.1556/004.2019.004
SP  - 34
EP  - 39
ER  - 

@article{
author = "Klun, Ivana and Ćosić, Nada and Ćirović, Duško and Vasilev, Dragan and Teodorović, Vlado and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6.2%, 95% CI = 4.0-8.4) animals, including 14 red foxes (4.7%), 7 wild cats (35%), 5 beech martens (4.8%), 2 pine martens (16.7%), and 1 European badger (6.25%). No Trichinella larvae were detected in the examined specimens of European polecats, steppe polecats and European otters. Species identification of the Trichinella larvae performed for 18 positive samples revealed T. spiralis in 77.8% and T. britovi in 22.2% of the isolates. Both species were detected in red foxes and wild cats. The predominance of T. spiralis in wildlife in Serbia indicates the (past or present) spillover of this pathogen from domestic to wild animals.",
journal = "Acta Veterinaria Hungarica, Acta Veterinaria Hungarica",
title = "Trichinella spp. in wild mesocarnivores in an endemic setting.",
number = "1",
volume = "67",
doi = "10.1556/004.2019.004",
pages = "34-39"
}

Klun, I., Ćosić, N., Ćirović, D., Vasilev, D., Teodorović, V.,& Đurković-Đaković, O.. (2019). Trichinella spp. in wild mesocarnivores in an endemic setting.. in Acta Veterinaria Hungarica, 67(1), 34-39.
https://doi.org/10.1556/004.2019.004

Klun I, Ćosić N, Ćirović D, Vasilev D, Teodorović V, Đurković-Đaković O. Trichinella spp. in wild mesocarnivores in an endemic setting.. in Acta Veterinaria Hungarica. 2019;67(1):34-39.
doi:10.1556/004.2019.004 .

Klun, Ivana, Ćosić, Nada, Ćirović, Duško, Vasilev, Dragan, Teodorović, Vlado, Đurković-Đaković, Olgica, "Trichinella spp. in wild mesocarnivores in an endemic setting." in Acta Veterinaria Hungarica, 67, no. 1 (2019):34-39,
https://doi.org/10.1556/004.2019.004 . .

TY  - JOUR
AU  - Savić, Ivo
AU  - Ćirović, Duško
AU  - Bugarski-Stanojević, Vanja
PY  - 2017
UR  - http://www.mdpi.com/2073-4425/8/11/292
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2895
AB  - Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.
T2  - Genes
T1  - Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe
IS  - 11
VL  - 8
DO  - 10.3390/genes8110292
SP  - 292
EP  - 292
ER  - 

@article{
author = "Savić, Ivo and Ćirović, Duško and Bugarski-Stanojević, Vanja",
year = "2017",
abstract = "Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.",
journal = "Genes",
title = "Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe",
number = "11",
volume = "8",
doi = "10.3390/genes8110292",
pages = "292-292"
}

Savić, I., Ćirović, D.,& Bugarski-Stanojević, V.. (2017). Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe. in Genes, 8(11), 292-292.
https://doi.org/10.3390/genes8110292

Savić I, Ćirović D, Bugarski-Stanojević V. Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe. in Genes. 2017;8(11):292-292.
doi:10.3390/genes8110292 .

Savić, Ivo, Ćirović, Duško, Bugarski-Stanojević, Vanja, "Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe" in Genes, 8, no. 11 (2017):292-292,
https://doi.org/10.3390/genes8110292 . .

TY  - JOUR
AU  - Jojić, Vida
AU  - Porobić, Jelena
AU  - Ćirović, Duško
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S004452311730013X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2561
AB  - We used geometric morphometric techniques to examine variability in Serbian red fox (Vulpes vulpes) crania. Male crania are about 5% larger than female ones and sexual size dimorphism (SSD) was significant. Also, significant cranial shape differences were found between the sexes. Males are characterized by reduced basicranial, but broader rostral and zygomatic regions. Although a slight and significant allometric effect was detected and larger specimens are smaller in the region of basicranium, cranial sexual shape dimorphism (SShD) pattern is not influenced by SSD. As small to moderate SSD in canid species is a general characteristics of the family, additional studies are needed to determine whether basicranial reduction in males, i.e. its enlargement in females, could be an important feature in characterizing cranial SShD patterns in other Canidae members. While uniform considering size, the shape of Serbian red fox crania varies geographically, as well as depending on proportion of agricultural habitats. In comparison to those from central and eastern Serbia, specimens from the northern Serbian region Vojvodina (with higher proportions of agricultural areas) have more robust crania with shorter snouts and maxillae, larger palatine bones accompanied with anteriorly moved posterior edges of the canine alveolus and laterally expanded zygomatic arches. Encompassing mostly facial and temporal cranial regions these shape changes are probably related to diet differences, although genetic diversification cannot be excluded as a possible contributing factor.
T2  - Zoologischer Anzeiger - A Journal of Comparative Zoology
T1  - Cranial variability of the Serbian red fox
VL  - 267
DO  - 10.1016/j.jcz.2017.02.001
SP  - 41
EP  - 48
ER  - 

@article{
author = "Jojić, Vida and Porobić, Jelena and Ćirović, Duško",
year = "2017",
abstract = "We used geometric morphometric techniques to examine variability in Serbian red fox (Vulpes vulpes) crania. Male crania are about 5% larger than female ones and sexual size dimorphism (SSD) was significant. Also, significant cranial shape differences were found between the sexes. Males are characterized by reduced basicranial, but broader rostral and zygomatic regions. Although a slight and significant allometric effect was detected and larger specimens are smaller in the region of basicranium, cranial sexual shape dimorphism (SShD) pattern is not influenced by SSD. As small to moderate SSD in canid species is a general characteristics of the family, additional studies are needed to determine whether basicranial reduction in males, i.e. its enlargement in females, could be an important feature in characterizing cranial SShD patterns in other Canidae members. While uniform considering size, the shape of Serbian red fox crania varies geographically, as well as depending on proportion of agricultural habitats. In comparison to those from central and eastern Serbia, specimens from the northern Serbian region Vojvodina (with higher proportions of agricultural areas) have more robust crania with shorter snouts and maxillae, larger palatine bones accompanied with anteriorly moved posterior edges of the canine alveolus and laterally expanded zygomatic arches. Encompassing mostly facial and temporal cranial regions these shape changes are probably related to diet differences, although genetic diversification cannot be excluded as a possible contributing factor.",
journal = "Zoologischer Anzeiger - A Journal of Comparative Zoology",
title = "Cranial variability of the Serbian red fox",
volume = "267",
doi = "10.1016/j.jcz.2017.02.001",
pages = "41-48"
}

Jojić, V., Porobić, J.,& Ćirović, D.. (2017). Cranial variability of the Serbian red fox. in Zoologischer Anzeiger - A Journal of Comparative Zoology, 267, 41-48.
https://doi.org/10.1016/j.jcz.2017.02.001

Jojić V, Porobić J, Ćirović D. Cranial variability of the Serbian red fox. in Zoologischer Anzeiger - A Journal of Comparative Zoology. 2017;267:41-48.
doi:10.1016/j.jcz.2017.02.001 .

Jojić, Vida, Porobić, Jelena, Ćirović, Duško, "Cranial variability of the Serbian red fox" in Zoologischer Anzeiger - A Journal of Comparative Zoology, 267 (2017):41-48,
https://doi.org/10.1016/j.jcz.2017.02.001 . .